Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Forward (+)

63318385 .. 63322458

Gene Structure 4074 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG465000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGCGCGTTGCGTCCCACGCGCCACCCTGATATCCACCGCGTCATTTCCGAAACTGGTCGTCCCTGGCAGAACGAATCGCGTCGAGGCCTCGAAATCTGCGCTATTCGGCCGGAAATTCCACGCGCTGTGCCGTTACGATCGGAGAGACGCTGCGAGGCCATTGGTGAGGACGCGAGTCTTCGCTGAGCAGAAGGAGAAGGAGCTCCAGGTCAATGTGAGCGTTAGCATCGAACAAGGTTTGCCTGAGGATCACGAAATATCGAGGATTGCCGAATTGCTTAGGCTAAATGTTCGAACTGCATTGAACCTAGCGTTTGGTGGATTGAAAGATTCCGGTGGTGGTTTTGAAAGCGCGGAGCTTTCGGTGATTCTGTGCAATGATGAGTTTATGAGAAAACGTAACAAGGAATGGAGAGACGAGGACCGTGCTACTGGTGTTGTTTCCAAGTCCAAGCATGTGCCTGGTCTTAAGATTCCAAATGTTGTGTTGGGTGACATTGTGATTTCTGTTGAGATGGCGGCGAGGCAAGCAGAGCAAAGAGGCCACACACTTCTTGATGAGATGCGCCTTCTCATGGTGCAGGGATTGTTACATTTATTGAGATCTGATCATGAGATCAGTCAAGAGGCTACAGGGGAAATGGAGAAGGAGGAGGAACGTCTTTTAGACAGTCTTGGGTGGAAGGGGAAAAGAATGGTTGCATCTGAAGCTGAAACCCATCCAAAAGCCAACGAGGGGAAAAGATGGAAGAAAGAAGGCAGCCTCCGACTCTATAAACCAAAGTTCAAATATGTGTTCTGTGACATGGATGGAACCCTCCTCAACAGCAAAAGTCAATTGAGTTCAACCACTGCCAGGGCTCTCAAAGAAGCCTCATCAAAGGGTGTGAAAATAGTGATTGCCACGGGTAAAGCTCGTCCAGCTGTGATGCGTGTCTTTAAGATGGTAGATTTAGCTGGAAAAGATGGCATTGTTTCAGAATTTTCTCCCGGGGTTTACTTGCAGGGATTGCTGGTTTATGGTGTTCAAGGTCAGGAAATTTGTAAAAGGAATTTAGATCCAAATGTCTGCAGAGAGGCATGTCTTTACTCATTGGAGAATAAGGTTCCTCTCATTGCATTCAGCAAGGACCGGTGCTCGAGTCTGTTTGATCACCCATATGTTGATGCTATGCATACTGTATATCATGAGCCAAAGGCAGAGATCATGCCTTCAGTTGAGCAGCTCTTGGCTGCTGCTGACATACAGAAACTGGTCTTCATGGACACTGCTGAGGGAGTATCTACTGCTCTGAGGCCGTATTGGTCAGAAGCAACTGGAGATCGTGCCACAGTTGTTCAAACTGTGCCGGACATGCTTGAACTTGTTCCCCCAGGAACCTCAAAAGGTAGTGGAGTAAAGATGTTGCTTGATCATTTGGGAATCACTGCAAAGGAGATCATGGCTATTGGCGATGGGGAGAATGATGTTGAGATGCTTGAGCTGGCTGGTTTGGGCATTTGTCTCAGTAATGGATCAGAGAAAACAAAAGCTGTGGCGAATGTAATCGGTGCCAGCAACGACGAAGATGGTGCTGCTGATGCAATCTACCGCTATGCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

535

Amino Acids

58.9

Weight (kDa)

7.16

Isoelectric Point (pI)

35.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	117 - 225	4e-23	Endoribonuclease YbeY
Hydrolase_3	PF08282	266 - 530	1.9e-50	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	436 - 498	6.8e-06	haloacid dehalogenase-like hydrolase
S6PP	PF05116	441 - 509	5.7e-08	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1553
AccII	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 356
AciI	CCGC	4 cut(s)	39, 356, 521, 1594
AclWI	GGATC	3 cut(s)	258, 1055, 1525
AcoI	YGGCCR	1 cut(s)	109
AcsI	RAATTY	4 cut(s)	116, 983, 1041, 1054
AcuI	CTGAAG	2 cut(s)	729, 1200
AfiI	CCNNNNNNNGG	3 cut(s)	577, 738, 858
AflII	CTTAAG	1 cut(s)	470
AgeI	ACCGGT	1 cut(s)	1134
AgsI	TTSAA	8 cut(s)	307, 328, 350, 790, 849, 1031, 1343, 1364
AjnI	CCWGG	5 cut(s)	64, 207, 463, 857, 1375
AluBI	AGCT	9 cut(s)	205, 361, 713, 917, 926, 959, 1228, 1486, 1535
AluI	AGCT	9 cut(s)	205, 361, 713, 917, 926, 959, 1228, 1486, 1535
Alw21I	GWGCWC	2 cut(s)	207, 1142
Alw26I	GTCTC	3 cut(s)	141, 411, 1511
AlwI	GGATC	3 cut(s)	258, 1055, 1525
AlwNI	CAGNNNCTG	1 cut(s)	1255
Ama87I	CYCGRG	2 cut(s)	992, 1141
AoxI	GGCC	5 cut(s)	87, 109, 158, 544, 1298
ApeKI	GCWGC	6 cut(s)	152, 762, 1225, 1235, 1238, 1577
ApoI	RAATTY	4 cut(s)	116, 983, 1041, 1054
AsiGI	ACCGGT	1 cut(s)	1134
Asp700I	GAANNNNTTC	1 cut(s)	1368
AspLEI	GCGC	6 cut(s)	7, 23, 103, 127, 356, 570
AspS9I	GGNCC	2 cut(s)	424, 1132
AsuC2I	CCSGG	2 cut(s)	993, 994
AsuHPI	GGTGA	4 cut(s)	178, 379, 506, 1148
AsuII	TTCGAA	1 cut(s)	295
AvaI	CYCGRG	2 cut(s)	992, 1141
AvaII	GGWCC	2 cut(s)	424, 1132
AxyI	CCTNAGG	1 cut(s)	246
BanI	GGYRCC	1 cut(s)	1553
BanII	GRGCYC	2 cut(s)	207, 865
BbsI	GAAGAC	2 cut(s)	172, 1252
Bbv12I	GWGCWC	2 cut(s)	207, 1142
BbvCI	CCTCAGC	1 cut(s)	1275
BbvI	GCAGC	6 cut(s)	139, 774, 1222, 1225, 1237, 1564
BccI	CCATC	8 cut(s)	511, 729, 741, 806, 940, 962, 1451, 1565
BceAI	ACGGC	2 cut(s)	117, 1285
BcgI	CGANNNNNNTGC	4 cut(s)	410, 444, 1556, 1590
BciT130I	CCWGG	5 cut(s)	66, 209, 465, 859, 1377
BclI	TGATCA	3 cut(s)	610, 1153, 1414
BcnI	CCSGG	2 cut(s)	993, 994
BcoDI	GTCTC	3 cut(s)	141, 411, 1511
BfaI	CTAG	1 cut(s)	311
BfmI	CTRYAG	2 cut(s)	633, 1072
BfrI	CTTAAG	1 cut(s)	470
BglII	AGATCT	1 cut(s)	605
BisI	GCNGC	7 cut(s)	153, 522, 763, 1226, 1236, 1239, 1578
BlpI	GCTNAGC	1 cut(s)	186
BlsI	GCNGC	7 cut(s)	154, 523, 764, 1227, 1237, 1240, 1579
Bme1390I	CCNGG	7 cut(s)	66, 209, 465, 859, 993, 994, 1377
Bme18I	GGWCC	2 cut(s)	424, 1132
BmeT110I	CYCGRG	2 cut(s)	992, 1141
BmgT120I	GGNCC	2 cut(s)	424, 1132
BmiI	GGNNCC	4 cut(s)	817, 1110, 1381, 1555
BmrFI	CCNGG	7 cut(s)	66, 209, 465, 859, 993, 994, 1377
BmsI	GCATC	7 cut(s)	237, 555, 713, 921, 1159, 1467, 1573
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	2 cut(s)	172, 1252
BpmI	CTGGAG	2 cut(s)	191, 1341
Bpu10I	CCTNAGC	2 cut(s)	281, 1275
Bpu1102I	GCTNAGC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	295
BpuEI	CTTGAG	1 cut(s)	1502
BpuMI	CCSGG	2 cut(s)	993, 994
BsaJI	CCNNGG	6 cut(s)	64, 858, 906, 992, 993, 1375
BsaWI	WCCGGW	2 cut(s)	335, 1134
Bsc4I	CCNNNNNNNGG	3 cut(s)	577, 738, 858
Bse118I	RCCGGY	1 cut(s)	1134
Bse1I	ACTGG	4 cut(s)	60, 439, 1260, 1324
Bse21I	CCTNAGG	1 cut(s)	246
Bse3DI	GCAATG	2 cut(s)	385, 1116
BseBI	CCWGG	5 cut(s)	66, 209, 465, 859, 1377
BseDI	CCNNGG	6 cut(s)	64, 858, 906, 992, 993, 1375
BseGI	GGATG	2 cut(s)	721, 817
BseLI	CCNNNNNNNGG	3 cut(s)	577, 738, 858
BseMI	GCAATG	2 cut(s)	385, 1116
BseMII	CTCAG	5 cut(s)	177, 237, 1266, 1286, 1522
BseNI	ACTGG	4 cut(s)	60, 439, 1260, 1324
BseRI	GAGGAG	2 cut(s)	668, 812
BseX3I	CGGCCG	1 cut(s)	109
BseXI	GCAGC	6 cut(s)	139, 774, 1222, 1225, 1237, 1564
BsgI	GTGCAG	1 cut(s)	602
Bsh1236I	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 356
Bsh1285I	CGRYCG	2 cut(s)	112, 142
BshFI	GGCC	5 cut(s)	89, 111, 160, 546, 1300
BshNI	GGYRCC	1 cut(s)	1553
BshTI	ACCGGT	1 cut(s)	1134
BsiEI	CGRYCG	2 cut(s)	112, 142
BsiHKAI	GWGCWC	2 cut(s)	207, 1142
BsiHKCI	CYCGRG	2 cut(s)	992, 1141
BsiSI	CCGG	5 cut(s)	112, 336, 993, 1135, 1352
BslFI	GGGAC	1 cut(s)	47
BslI	CCNNNNNNNGG	3 cut(s)	577, 738, 858
BsmAI	GTCTC	3 cut(s)	141, 411, 1511
BsmBI	CGTCTC	2 cut(s)	141, 411
BsmFI	GGGAC	1 cut(s)	47
BsmI	GAATGC	1 cut(s)	1121
BsnI	GGCC	5 cut(s)	89, 111, 160, 546, 1300
BsoBI	CYCGRG	2 cut(s)	992, 1141
Bsp119I	TTCGAA	1 cut(s)	295
Bsp1286I	GDGCHC	3 cut(s)	207, 865, 1142
Bsp1720I	GCTNAGC	1 cut(s)	186
BspACI	CCGC	4 cut(s)	39, 356, 521, 1594
BspANI	GGCC	5 cut(s)	89, 111, 160, 546, 1300
BspCNI	CTCAG	5 cut(s)	178, 238, 1267, 1287, 1521
BspFNI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 356
BspHI	TCATGA	2 cut(s)	613, 1189
BspLI	GGNNCC	4 cut(s)	817, 1110, 1381, 1555
BspMAI	CTGCAG	1 cut(s)	1076
BspPI	GGATC	3 cut(s)	258, 1055, 1525
BspT104I	TTCGAA	1 cut(s)	295
BspT107I	GGYRCC	1 cut(s)	1553
BspTI	CTTAAG	1 cut(s)	470
BsrDI	GCAATG	2 cut(s)	385, 1116
BsrFI	RCCGGY	1 cut(s)	1134
BsrI	ACTGG	4 cut(s)	60, 439, 1260, 1324
BssAI	RCCGGY	1 cut(s)	1134
BssECI	CCNNGG	6 cut(s)	64, 858, 906, 992, 993, 1375
Bst2UI	CCWGG	5 cut(s)	66, 209, 465, 859, 1377
Bst4CI	ACNGT	5 cut(s)	428, 674, 1183, 1336, 1348
BstAFI	CTTAAG	1 cut(s)	470
BstAPI	GCANNNNNTGC	1 cut(s)	1012
BstBI	TTCGAA	1 cut(s)	295
BstC8I	GCNNGC	3 cut(s)	531, 1488, 1558
BstDEI	CTNAG	6 cut(s)	186, 246, 281, 1275, 1295, 1508
BstDSI	CCRYGG	1 cut(s)	906
BstF5I	GGATG	2 cut(s)	721, 817
BstFNI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 356
BstHHI	GCGC	6 cut(s)	7, 23, 103, 127, 356, 570
BstMAI	GTCTC	3 cut(s)	141, 411, 1511
BstMCI	CGRYCG	2 cut(s)	112, 142
BstMWI	GCNNNNNNNGC	8 cut(s)	18, 530, 535, 710, 923, 1012, 1357, 1583
BstNI	CCWGG	5 cut(s)	66, 209, 465, 859, 1377
BstNSI	RCATGY	3 cut(s)	461, 1086, 1360
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSCI	CCNGG	7 cut(s)	64, 207, 463, 857, 991, 992, 1375
BstSFI	CTRYAG	2 cut(s)	633, 1072
BstUI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 356
BstV1I	GCAGC	6 cut(s)	139, 774, 1222, 1225, 1237, 1564
BstV2I	GAAGAC	2 cut(s)	172, 1252
BstX2I	RGATCY	2 cut(s)	605, 1060
BstYI	RGATCY	2 cut(s)	605, 1060
BstZI	CGGCCG	1 cut(s)	109
Bsu36I	CCTNAGG	1 cut(s)	246
BsuRI	GGCC	5 cut(s)	89, 111, 160, 546, 1300
BtgI	CCRYGG	1 cut(s)	906
BtgZI	GCGATG	1 cut(s)	1470
BtsCI	GGATG	2 cut(s)	721, 817
BtsI	GCAGTG	3 cut(s)	852, 1269, 1428
BtsIMutI	CAGTG	3 cut(s)	852, 1269, 1428
Cac8I	GCNNGC	3 cut(s)	531, 1488, 1558
CaiI	CAGNNNCTG	1 cut(s)	1255
CciI	TCATGA	2 cut(s)	613, 1189
CfoI	GCGC	6 cut(s)	7, 23, 103, 127, 356, 570
Cfr10I	RCCGGY	1 cut(s)	1134
Cfr13I	GGNCC	2 cut(s)	424, 1132
Cfr9I	CCCGGG	1 cut(s)	992
CseI	GACGC	4 cut(s)	30, 70, 158, 181
CspAI	ACCGGT	1 cut(s)	1134
CspCI	CAANNNNNGTGG	2 cut(s)	1518, 1553
DdeI	CTNAG	6 cut(s)	186, 246, 281, 1275, 1295, 1508
EaeI	YGGCCR	1 cut(s)	109
EagI	CGGCCG	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	205
EclXI	CGGCCG	1 cut(s)	109
Eco147I	AGGCCT	1 cut(s)	89
Eco24I	GRGCYC	2 cut(s)	207, 865
Eco32I	GATATC	1 cut(s)	33
Eco47I	GGWCC	2 cut(s)	424, 1132
Eco52I	CGGCCG	1 cut(s)	109
Eco53kI	GAGCTC	1 cut(s)	205
Eco57I	CTGAAG	2 cut(s)	729, 1200
Eco81I	CCTNAGG	1 cut(s)	246
Eco88I	CYCGRG	2 cut(s)	992, 1141
EcoICRI	GAGCTC	1 cut(s)	205
EcoRII	CCWGG	5 cut(s)	64, 207, 463, 857, 1375
EcoRV	GATATC	1 cut(s)	33
EcoT22I	ATGCAT	2 cut(s)	1179, 1603
EcoT38I	GRGCYC	2 cut(s)	207, 865
Esp3I	CGTCTC	2 cut(s)	141, 411
FaqI	GGGAC	1 cut(s)	47
FauNDI	CATATG	1 cut(s)	1162
FbaI	TGATCA	3 cut(s)	610, 1153, 1414
Fnu4HI	GCNGC	7 cut(s)	153, 522, 763, 1226, 1236, 1239, 1578
FokI	GGATG	2 cut(s)	708, 824
FriOI	GRGCYC	2 cut(s)	207, 865
Fsp4HI	GCNGC	7 cut(s)	153, 522, 763, 1226, 1236, 1239, 1578
FspBI	CTAG	1 cut(s)	311
GlaI	GCGC	6 cut(s)	6, 22, 102, 126, 355, 569
GluI	GCNGC	7 cut(s)	153, 522, 763, 1226, 1236, 1239, 1578
GsuI	CTGGAG	2 cut(s)	191, 1341
HaeIII	GGCC	5 cut(s)	89, 111, 160, 546, 1300
HapII	CCGG	5 cut(s)	112, 336, 993, 1135, 1352
HgaI	GACGC	4 cut(s)	30, 70, 158, 181
HhaI	GCGC	6 cut(s)	7, 23, 103, 127, 356, 570
Hin6I	GCGC	6 cut(s)	5, 21, 101, 125, 354, 568
HinP1I	GCGC	6 cut(s)	5, 21, 101, 125, 354, 568
HinfI	GANTC	8 cut(s)	76, 177, 332, 370, 475, 771, 1144, 1425
HpaII	CCGG	5 cut(s)	112, 336, 993, 1135, 1352
HphI	GGTGA	4 cut(s)	178, 379, 506, 1148
Hpy166II	GTNNAC	1 cut(s)	1000
Hpy188I	TCNGA	9 cut(s)	51, 144, 610, 709, 770, 982, 1296, 1312, 1522
Hpy188III	TCNNGA	6 cut(s)	254, 557, 614, 626, 1037, 1190
Hpy8I	GTNNAC	1 cut(s)	1000
Hpy99I	CGWCG	2 cut(s)	86, 1568
HpyAV	CCTTC	7 cut(s)	187, 193, 581, 643, 679, 752, 1224
HpyCH4III	ACNGT	5 cut(s)	428, 674, 1183, 1336, 1348
HpyCH4IV	ACGT	2 cut(s)	400, 661
HpyF10VI	GCNNNNNNNGC	8 cut(s)	18, 530, 535, 710, 923, 1012, 1357, 1583
HpyF3I	CTNAG	6 cut(s)	186, 246, 281, 1275, 1295, 1508
HpySE526I	ACGT	2 cut(s)	400, 661
HspAI	GCGC	6 cut(s)	5, 21, 101, 125, 354, 568
Ksp22I	TGATCA	3 cut(s)	610, 1153, 1414
LmnI	GCTCC	3 cut(s)	202, 210, 358
Lsp1109I	GCAGC	6 cut(s)	139, 774, 1222, 1225, 1237, 1564
LweI	GCATC	7 cut(s)	237, 555, 713, 921, 1159, 1467, 1573
MaeI	CTAG	1 cut(s)	311
MaeII	ACGT	2 cut(s)	400, 661
MaeIII	GTNAC	5 cut(s)	134, 401, 494, 591, 803
MboII	GAAGA	4 cut(s)	172, 763, 1252, 1580
MfeI	CAATTG	1 cut(s)	839
MflI	RGATCY	2 cut(s)	605, 1060
MhlI	GDGCHC	3 cut(s)	207, 865, 1142
MluCI	AATT	6 cut(s)	116, 275, 839, 983, 1041, 1054
MlyI	GAGTC	3 cut(s)	186, 765, 1153
MmeI	TCCRAC	1 cut(s)	793
Mph1103I	ATGCAT	2 cut(s)	1179, 1603
MroXI	GAANNNNTTC	1 cut(s)	1368
MseI	TTAA	2 cut(s)	471, 942
MslI	CAYNNNNRTG	1 cut(s)	1161
MspA1I	CMGCKG	1 cut(s)	926
MspCI	CTTAAG	1 cut(s)	470
MspI	CCGG	5 cut(s)	112, 336, 993, 1135, 1352
MspR9I	CCNGG	7 cut(s)	66, 209, 465, 859, 993, 994, 1377
MunI	CAATTG	1 cut(s)	839
Mva1269I	GAATGC	1 cut(s)	1121
MvaI	CCWGG	5 cut(s)	66, 209, 465, 859, 1377
MvnI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 356
MwoI	GCNNNNNNNGC	8 cut(s)	18, 530, 535, 710, 923, 1012, 1357, 1583
NciI	CCSGG	2 cut(s)	993, 994
NdeI	CATATG	1 cut(s)	1162
NlaIV	GGNNCC	4 cut(s)	817, 1110, 1381, 1555
NmuCI	GTSAC	2 cut(s)	494, 803
NsiI	ATGCAT	2 cut(s)	1179, 1603
NspI	RCATGY	3 cut(s)	461, 1086, 1360
NspV	TTCGAA	1 cut(s)	295
PaeR7I	CTCGAG	1 cut(s)	1141
PagI	TCATGA	2 cut(s)	613, 1189
PceI	AGGCCT	1 cut(s)	89
PctI	GAATGC	1 cut(s)	1121
PdmI	GAANNNNTTC	1 cut(s)	1368
PfeI	GAWTC	5 cut(s)	76, 332, 370, 475, 1425
PinAI	ACCGGT	1 cut(s)	1134
PkrI	GCNGC	7 cut(s)	154, 523, 764, 1227, 1237, 1240, 1579
Ple19I	CGATCG	1 cut(s)	142
PleI	GAGTC	3 cut(s)	185, 765, 1152
PpsI	GAGTC	3 cut(s)	185, 765, 1152
PshAI	GACNNNNGTC	1 cut(s)	176
Psp124BI	GAGCTC	1 cut(s)	207
Psp6I	CCWGG	5 cut(s)	64, 207, 463, 857, 1375
PspGI	CCWGG	5 cut(s)	64, 207, 463, 857, 1375
PspN4I	GGNNCC	4 cut(s)	817, 1110, 1381, 1555
PspPI	GGNCC	2 cut(s)	424, 1132
PspXI	VCTCGAGB	1 cut(s)	1141
PstI	CTGCAG	1 cut(s)	1076
PstNI	CAGNNNCTG	1 cut(s)	1255
PsuI	RGATCY	2 cut(s)	605, 1060
PvuI	CGATCG	1 cut(s)	142
PvuII	CAGCTG	1 cut(s)	926
RseI	CAYNNNNRTG	1 cut(s)	1161
SacI	GAGCTC	1 cut(s)	207
SaqAI	TTAA	2 cut(s)	471, 942
SatI	GCNGC	7 cut(s)	153, 522, 763, 1226, 1236, 1239, 1578
Sau96I	GGNCC	2 cut(s)	424, 1132
SchI	GAGTC	3 cut(s)	186, 765, 1153
ScrFI	CCNGG	7 cut(s)	66, 209, 465, 859, 993, 994, 1377
SduI	GDGCHC	3 cut(s)	207, 865, 1142
SfaNI	GCATC	7 cut(s)	237, 555, 713, 921, 1159, 1467, 1573
SfcI	CTRYAG	2 cut(s)	633, 1072
Sfr274I	CTCGAG	1 cut(s)	1141
SfuI	TTCGAA	1 cut(s)	295
SinI	GGWCC	2 cut(s)	424, 1132
SlaI	CTCGAG	1 cut(s)	1141
SmaI	CCCGGG	1 cut(s)	994
SmiMI	CAYNNNNRTG	1 cut(s)	1161
SmlI	CTYRAG	3 cut(s)	470, 1141, 1481
SmoI	CTYRAG	3 cut(s)	470, 1141, 1481
Sse9I	AATT	6 cut(s)	116, 275, 839, 983, 1041, 1054
SseBI	AGGCCT	1 cut(s)	89
SsiI	CCGC	4 cut(s)	39, 356, 521, 1594
SspMI	CTAG	1 cut(s)	311
SstI	GAGCTC	1 cut(s)	207
StuI	AGGCCT	1 cut(s)	89
StyD4I	CCNGG	7 cut(s)	64, 207, 463, 857, 991, 992, 1375
TaaI	ACNGT	5 cut(s)	428, 674, 1183, 1336, 1348
TaiI	ACGT	2 cut(s)	403, 664
TaqI	TCGA	6 cut(s)	84, 92, 231, 263, 295, 1142
TasI	AATT	6 cut(s)	116, 275, 839, 983, 1041, 1054
TauI	GCSGC	1 cut(s)	524
TfiI	GAWTC	5 cut(s)	76, 332, 370, 475, 1425
Tru1I	TTAA	2 cut(s)	471, 942
Tru9I	TTAA	2 cut(s)	471, 942
TscAI	CASTG	3 cut(s)	859, 1276, 1435
TseFI	GTSAC	2 cut(s)	494, 803
TseI	GCWGC	6 cut(s)	152, 762, 1225, 1235, 1238, 1577
Tsp45I	GTSAC	2 cut(s)	494, 803
TspDTI	ATGAA	1 cut(s)	1252
TspMI	CCCGGG	1 cut(s)	992
TspRI	CASTG	3 cut(s)	859, 1276, 1435
Vha464I	CTTAAG	1 cut(s)	470
VpaK11BI	GGWCC	2 cut(s)	424, 1132
XapI	RAATTY	4 cut(s)	116, 983, 1041, 1054
XceI	RCATGY	3 cut(s)	461, 1086, 1360
XhoI	CTCGAG	1 cut(s)	1141
XmaI	CCCGGG	1 cut(s)	992
XmnI	GAANNNNTTC	1 cut(s)	1368
XspI	CTAG	1 cut(s)	311
Zsp2I	ATGCAT	2 cut(s)	1179, 1603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.