MD08G1151300.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003240
Physical Location & Seq
Reverse (-)
15483655 .. 15485406
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1151300.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGAGCTTCAGTTAGGTCTGGCTCTAGCTCTTCCTGACCATCACAGTCCTGTCAAAGGTTTTGACATGAATGGCGTTAATCGCAGCGTCGAGAAAAAAGAGATGGTGGGTTTGGAGAGCAAAACCCAAGTTGGGAATTACAAGCGTAGTTTTGATGTGGCTTTTGAGAAGAGTAGTAGTACTGGTGCTGGAAATGAATCGAAGATGTTGGCTTTGTTGTTGTGGAATGATCAGCCAAATGAGGAAGATGATGATGATAAGGGGCGAAAGAGAAGAAATTCTTGCAGGTCCATAATCAAGAATGATGATGGAGGAAATCATGTAGTTGGTTGGCCACCAATTAAATCTTGGAGGAAAAAGATGCATCATGATCATGATCAACACCATCAGTATTACCCTCTCCAGAATAATCAGATGGCTAATAATTATAAGGAAAACGAAAACGATGGCTCAGCAGCAGCAGCGAATAATTCCATGTACGTGAAGGTGAAGATGGAAGGAGAGGGAATTGTGAGGAAGATCGACATAAATCTGCATCATTCTTTTCAGTCGCTCAGAGACACCTTGATTACCATGTTTTCCAAATGCAAAAGCAAAGAGGGTGGTGCTGCAGCTGATTATATACTCATTTATCAGGACAAACAAGGAGATTGGCTGCTCGCTGCAGATGTTCCCTGGCAAACCTTCATCGAGTCCGTGCAGCGCCTGCAGATAGTTAGGAATGGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

244

Amino Acids

27.54

Weight (kDa)

6.8

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 2 - 241 7.2e-35 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 429
Acc36I ACCTGC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 332
AcsI RAATTY 1 cut(s) 277
AfaI GTAC 2 cut(s) 181, 479
AfiI CCNNNNNNNGG 2 cut(s) 56, 132
AjnI CCWGG 1 cut(s) 674
AluBI AGCT 3 cut(s) 7, 29, 614
AluI AGCT 3 cut(s) 7, 29, 614
Alw26I GTCTC 1 cut(s) 552
AlwNI CAGNNNCTG 1 cut(s) 706
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 9 cut(s) 84, 455, 458, 461, 608, 611, 655, 662, 700
ApoI RAATTY 1 cut(s) 277
Asp700I GAANNNNTTC 1 cut(s) 277
AspLEI GCGC 1 cut(s) 705
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 288
BalI TGGCCA 1 cut(s) 334
BbvI GCAGC 9 cut(s) 96, 467, 470, 473, 595, 623, 642, 649, 712
BccI CCATC 7 cut(s) 48, 97, 302, 393, 409, 440, 487
BciT130I CCWGG 1 cut(s) 676
BclI TGATCA 3 cut(s) 229, 370, 376
BcoDI GTCTC 1 cut(s) 552
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 3 cut(s) 609, 663, 707
BfoI RGCGCY 1 cut(s) 706
BfuAI ACCTGC 1 cut(s) 276
BisI GCNGC 9 cut(s) 85, 456, 459, 462, 609, 612, 656, 663, 701
BlpI GCTNAGC 1 cut(s) 451
BlsI GCNGC 9 cut(s) 86, 457, 460, 463, 610, 613, 657, 664, 702
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 676
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 676
BmsI GCATC 3 cut(s) 351, 373, 544
BpmI CTGGAG 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 451
BsaAI YACGTR 1 cut(s) 481
BsaBI GATNNNNATC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 674
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 132
Bse1I ACTGG 1 cut(s) 187
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 1 cut(s) 676
BseDI CCNNGG 1 cut(s) 674
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 56, 132
BseMII CTCAG 2 cut(s) 465, 568
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 9 cut(s) 96, 467, 470, 473, 595, 623, 642, 649, 712
BsgI GTGCAG 1 cut(s) 719
BshFI GGCC 1 cut(s) 334
BslI CCNNNNNNNGG 2 cut(s) 56, 132
BsmAI GTCTC 1 cut(s) 552
BsnI GGCC 1 cut(s) 334
Bsp143I GATC 4 cut(s) 229, 370, 376, 519
Bsp1720I GCTNAGC 1 cut(s) 451
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 2 cut(s) 464, 567
BspHI TCATGA 2 cut(s) 367, 373
BspMAI CTGCAG 3 cut(s) 613, 667, 711
BspMI ACCTGC 1 cut(s) 276
BspQI GCTCTTC 1 cut(s) 36
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 674
BssMI GATC 4 cut(s) 229, 370, 376, 519
Bst2UI CCWGG 1 cut(s) 676
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 2 cut(s) 36, 164
BstAPI GCANNNNNTGC 1 cut(s) 706
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 660, 707
BstDEI CTNAG 2 cut(s) 451, 554
BstENI CCTNNNNNAGG 1 cut(s) 54
BstH2I RGCGCY 1 cut(s) 706
BstHHI GCGC 1 cut(s) 705
BstKTI GATC 4 cut(s) 232, 373, 379, 522
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 4 cut(s) 229, 370, 376, 519
BstMWI GCNNNNNNNGC 3 cut(s) 81, 461, 706
BstNI CCWGG 1 cut(s) 676
BstSCI CCNGG 1 cut(s) 674
BstSFI CTRYAG 3 cut(s) 609, 663, 707
BstV1I GCAGC 9 cut(s) 96, 467, 470, 473, 595, 623, 642, 649, 712
BsuRI GGCC 1 cut(s) 334
BveI ACCTGC 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 660, 707
CaiI CAGNNNCTG 1 cut(s) 706
CciI TCATGA 2 cut(s) 367, 373
CfoI GCGC 1 cut(s) 705
Cfr13I GGNCC 1 cut(s) 288
CseI GACGC 1 cut(s) 76
Csp6I GTAC 2 cut(s) 180, 478
CspCI CAANNNNNGTGG 2 cut(s) 583, 618
CviAII CATG 6 cut(s) 67, 320, 368, 374, 475, 574
CviQI GTAC 2 cut(s) 180, 478
DdeI CTNAG 2 cut(s) 451, 554
DpnI GATC 4 cut(s) 231, 372, 378, 521
DpnII GATC 4 cut(s) 229, 370, 376, 519
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 36, 164
EarI CTCTTC 2 cut(s) 36, 164
Eco47I GGWCC 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 674
EcoT22I ATGCAT 1 cut(s) 366
FaeI CATG 6 cut(s) 70, 323, 371, 377, 478, 577
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FatI CATG 6 cut(s) 66, 319, 367, 373, 474, 573
FbaI TGATCA 3 cut(s) 229, 370, 376
Fnu4HI GCNGC 9 cut(s) 85, 456, 459, 462, 609, 612, 656, 663, 701
Fsp4HI GCNGC 9 cut(s) 85, 456, 459, 462, 609, 612, 656, 663, 701
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 704
GluI GCNGC 9 cut(s) 85, 456, 459, 462, 609, 612, 656, 663, 701
GsuI CTGGAG 1 cut(s) 386
HaeII RGCGCY 1 cut(s) 706
HaeIII GGCC 1 cut(s) 334
HgaI GACGC 1 cut(s) 76
HhaI GCGC 1 cut(s) 705
Hin1II CATG 6 cut(s) 70, 323, 371, 377, 478, 577
Hin6I GCGC 1 cut(s) 703
HinP1I GCGC 1 cut(s) 703
HinfI GANTC 2 cut(s) 197, 692
HphI GGTGA 1 cut(s) 499
Hpy188I TCNGA 2 cut(s) 414, 557
Hpy188III TCNNGA 7 cut(s) 35, 91, 298, 368, 374, 403, 635
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 478, 491, 694
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 8 cut(s) 285, 364, 535, 588, 611, 665, 700, 709
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 461, 706
HpyF3I CTNAG 2 cut(s) 451, 554
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 6 cut(s) 70, 323, 371, 377, 478, 577
HspAI GCGC 1 cut(s) 703
Ksp22I TGATCA 3 cut(s) 229, 370, 376
Kzo9I GATC 4 cut(s) 229, 370, 376, 519
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 9 cut(s) 96, 467, 470, 473, 595, 623, 642, 649, 712
LweI GCATC 3 cut(s) 351, 373, 544
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 480
MalI GATC 4 cut(s) 231, 372, 378, 521
MboI GATC 4 cut(s) 229, 370, 376, 519
MboII GAAGA 7 cut(s) 23, 181, 214, 257, 285, 502, 529
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 6 cut(s) 136, 277, 339, 424, 469, 507
MluNI TGGCCA 1 cut(s) 334
MlyI GAGTC 1 cut(s) 701
MnlI CCTC 7 cut(s) 235, 305, 345, 408, 496, 507, 592
Mox20I TGGCCA 1 cut(s) 334
Mph1103I ATGCAT 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 277
MscI TGGCCA 1 cut(s) 334
MseI TTAA 2 cut(s) 78, 342
MslI CAYNNNNRTG 2 cut(s) 372, 479
Msp20I TGGCCA 1 cut(s) 334
MspA1I CMGCKG 1 cut(s) 614
MspR9I CCNGG 1 cut(s) 676
MvaI CCWGG 1 cut(s) 676
MwoI GCNNNNNNNGC 3 cut(s) 81, 461, 706
NdeII GATC 4 cut(s) 229, 370, 376, 519
NlaIII CATG 6 cut(s) 70, 323, 371, 377, 478, 577
NsiI ATGCAT 1 cut(s) 366
PagI TCATGA 2 cut(s) 367, 373
PciSI GCTCTTC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 277
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 9 cut(s) 86, 457, 460, 463, 610, 613, 657, 664, 702
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Ppu21I YACGTR 1 cut(s) 481
PsiI TTATAA 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 674
PspGI CCWGG 1 cut(s) 674
PspPI GGNCC 1 cut(s) 288
PstI CTGCAG 3 cut(s) 613, 667, 711
PstNI CAGNNNCTG 1 cut(s) 706
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 181, 479
RsaNI GTAC 2 cut(s) 180, 478
RseI CAYNNNNRTG 2 cut(s) 372, 479
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 2 cut(s) 78, 342
SatI GCNGC 9 cut(s) 85, 456, 459, 462, 609, 612, 656, 663, 701
Sau3AI GATC 4 cut(s) 229, 370, 376, 519
Sau96I GGNCC 1 cut(s) 288
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 1 cut(s) 676
SfaNI GCATC 3 cut(s) 351, 373, 544
SfcI CTRYAG 3 cut(s) 609, 663, 707
SinI GGWCC 1 cut(s) 288
SmiMI CAYNNNNRTG 2 cut(s) 372, 479
Sse9I AATT 6 cut(s) 136, 277, 339, 424, 469, 507
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 674
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 1 cut(s) 483
TaqI TCGA 4 cut(s) 90, 200, 522, 690
TasI AATT 6 cut(s) 136, 277, 339, 424, 469, 507
TatI WGTACW 1 cut(s) 179
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 2 cut(s) 78, 342
Tru9I TTAA 2 cut(s) 78, 342
TseI GCWGC 9 cut(s) 84, 455, 458, 461, 608, 611, 655, 662, 700
TspDTI ATGAA 3 cut(s) 83, 210, 676
TspGWI ACGGA 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 288
XagI CCTNNNNNAGG 1 cut(s) 54
XapI RAATTY 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 277
XspI CTAG 1 cut(s) 26
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.