Rh2DG032700

Glycosyltransferase family 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003240
Physical Location & Seq
Forward (+)
2096537 .. 2098336
1800 bp
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UTR
Exon/CDS
Intron
Rh2DG032700.1

Sequence Viewer

Length: 654 bp
ATGGAGCTTCAGCTGGGTCTCGCTCTTCCAACCAATAACAATTTTCCCGTCAAAGGCGGCCTCGACTTGAACATTAACGACTTTCTCTGCCACCCTACTATTAGTAGCGGCAGCAGCAACAAGAACAAGAAGCGTAGTTTCACCCAAGCTTTTGAGCCCAGCAATGCCTCAGCAGCCGTGCCTCGAACGCTGCCTTGGTTGCTTTGGAACAACCAACCAAACGACGACGACGACGAAGATGACCCCAAACATAGTTCTCGTTATCACACCATTATGATCAAAAATGAGGAAGATCGACTGGTGGGTTGGCCGCCCATTAAGTCATGGAAGAAGGAGACTTGCTTTCACATCCACGGCGACGGCGGTGGCCGTCGTCATCGGACAGTGGGGATTAATGGCGGAAACTCTACATTTGTGAAGGTGAAAATGGAGGGAGTTGGAATAGCAAGAAAGATTGACTTGAGCCAACATCAAAATTTCCAAACACTCCTAAGAACCTTGATGCACATGTTTGACAAATGTGAGATAGATTCAGACAATTATAAACTCACTTATCAAGACAGGGAAGGAGACTGGCTTCTTGCTCATGATCAAGATGTACCTTGGAGGTATAAAAAAAAATGCCCTCTAATACTATTATTAATTATAAGATGA

Protein Analysis

217

Amino Acids

24.92

Weight (kDa)

8.31

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 2 - 175 1.4e-18 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 543, 647
AciI CCGC 5 cut(s) 57, 108, 311, 363, 399
AcoI YGGCCR 2 cut(s) 308, 367
AcsI RAATTY 1 cut(s) 475
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 1 cut(s) 70
AjuI GAANNNNNNNTTGG 2 cut(s) 178, 210
AluBI AGCT 3 cut(s) 7, 13, 149
AluI AGCT 3 cut(s) 7, 13, 149
Alw26I GTCTC 3 cut(s) 23, 329, 564
AoxI GGCC 3 cut(s) 58, 308, 367
ApeKI GCWGC 4 cut(s) 111, 114, 173, 190
ApoI RAATTY 1 cut(s) 475
AseI ATTAAT 2 cut(s) 393, 641
AsuHPI GGTGA 2 cut(s) 133, 433
BanII GRGCYC 1 cut(s) 159
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 4 cut(s) 123, 126, 177, 185
BceAI ACGGC 4 cut(s) 161, 354, 370, 376
BclI TGATCA 2 cut(s) 276, 589
BcoDI GTCTC 3 cut(s) 23, 329, 564
BisI GCNGC 7 cut(s) 58, 109, 112, 115, 174, 191, 311
BlsI GCNGC 7 cut(s) 59, 110, 113, 116, 175, 192, 312
BmsI GCATC 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 481
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 194, 352, 602
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 303, 578
Bse3DI GCAATG 1 cut(s) 169
BseDI CCNNGG 3 cut(s) 194, 352, 602
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 303, 578
BseXI GCAGC 4 cut(s) 123, 126, 177, 185
BseYI CCCAGC 2 cut(s) 13, 158
BshFI GGCC 3 cut(s) 60, 310, 369
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 3 cut(s) 23, 329, 564
BsnI GGCC 3 cut(s) 60, 310, 369
Bso31I GGTCTC 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 3 cut(s) 276, 292, 589
BspACI CCGC 5 cut(s) 57, 108, 311, 363, 399
BspANI GGCC 3 cut(s) 60, 310, 369
BspCNI CTCAG 1 cut(s) 182
BspHI TCATGA 1 cut(s) 586
BspQI GCTCTTC 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 2 cut(s) 303, 578
BssECI CCNNGG 3 cut(s) 194, 352, 602
BssMI GATC 3 cut(s) 276, 292, 589
BssT1I CCWWGG 2 cut(s) 194, 602
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 30
BstDEI CTNAG 2 cut(s) 169, 491
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 3 cut(s) 279, 295, 592
BstMAI GTCTC 3 cut(s) 23, 329, 564
BstMBI GATC 3 cut(s) 276, 292, 589
BstMWI GCNNNNNNNGC 4 cut(s) 114, 173, 187, 199
BstNSI RCATGY 1 cut(s) 511
BstV1I GCAGC 4 cut(s) 123, 126, 177, 185
BsuRI GGCC 3 cut(s) 60, 310, 369
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 390
CciI TCATGA 1 cut(s) 586
Csp6I GTAC 1 cut(s) 599
CviAII CATG 3 cut(s) 324, 508, 587
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 2 cut(s) 169, 491
DpnI GATC 3 cut(s) 278, 294, 591
DpnII GATC 3 cut(s) 276, 292, 589
EaeI YGGCCR 2 cut(s) 308, 367
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 194, 602
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 23
EcoT14I CCWWGG 2 cut(s) 194, 602
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 194, 602
FaeI CATG 3 cut(s) 327, 511, 590
FaiI YATR 8 cut(s) 252, 275, 325, 509, 543, 588, 612, 647
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 3 cut(s) 323, 507, 586
FbaI TGATCA 2 cut(s) 276, 589
Fnu4HI GCNGC 7 cut(s) 58, 109, 112, 115, 174, 191, 311
FokI GGATG 1 cut(s) 335
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 7 cut(s) 58, 109, 112, 115, 174, 191, 311
GluI GCNGC 7 cut(s) 58, 109, 112, 115, 174, 191, 311
GsaI CCCAGC 2 cut(s) 17, 162
HaeIII GGCC 3 cut(s) 60, 310, 369
Hin1II CATG 3 cut(s) 327, 511, 590
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 1 cut(s) 530
HphI GGTGA 2 cut(s) 133, 433
Hpy188I TCNGA 2 cut(s) 381, 535
Hpy188III TCNNGA 3 cut(s) 557, 587, 593
Hpy99I CGWCG 6 cut(s) 227, 230, 233, 236, 362, 375
HpyAV CCTTC 3 cut(s) 325, 412, 560
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4V TGCA 1 cut(s) 505
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 173, 187, 199
HpyF3I CTNAG 2 cut(s) 169, 491
Hsp92II CATG 3 cut(s) 327, 511, 590
Ksp22I TGATCA 2 cut(s) 276, 589
Kzo9I GATC 3 cut(s) 276, 292, 589
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 172, 284, 547, 559
Lsp1109I GCAGC 4 cut(s) 123, 126, 177, 185
LweI GCATC 1 cut(s) 492
MalI GATC 3 cut(s) 278, 294, 591
MboI GATC 3 cut(s) 276, 292, 589
MboII GAAGA 4 cut(s) 17, 248, 302, 340
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 4 cut(s) 40, 475, 538, 642
MmeI TCCRAC 2 cut(s) 53, 418
MnlI CCTC 7 cut(s) 71, 178, 192, 280, 424, 600, 636
MseI TTAA 4 cut(s) 75, 318, 393, 641
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 13
MwoI GCNNNNNNNGC 4 cut(s) 114, 173, 187, 199
NdeII GATC 3 cut(s) 276, 292, 589
NlaIII CATG 3 cut(s) 327, 511, 590
NspI RCATGY 1 cut(s) 511
PagI TCATGA 1 cut(s) 586
PciI ACATGT 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 30
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 231
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 7 cut(s) 59, 110, 113, 116, 175, 192, 312
PscI ACATGT 1 cut(s) 507
PshBI ATTAAT 2 cut(s) 393, 641
PsiI TTATAA 2 cut(s) 543, 647
PspFI CCCAGC 2 cut(s) 13, 158
PvuII CAGCTG 1 cut(s) 13
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 4 cut(s) 75, 318, 393, 641
SatI GCNGC 7 cut(s) 58, 109, 112, 115, 174, 191, 311
Sau3AI GATC 3 cut(s) 276, 292, 589
SduI GDGCHC 1 cut(s) 159
SetI ASST 7 cut(s) 9, 15, 151, 423, 500, 604, 611
SfaNI GCATC 1 cut(s) 492
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 460
SmoI CTYRAG 1 cut(s) 460
Sse9I AATT 4 cut(s) 40, 475, 538, 642
SsiI CCGC 5 cut(s) 57, 108, 311, 363, 399
StyI CCWWGG 2 cut(s) 194, 602
TaaI ACNGT 1 cut(s) 385
TaqI TCGA 3 cut(s) 63, 184, 295
TasI AATT 4 cut(s) 40, 475, 538, 642
TauI GCSGC 3 cut(s) 60, 111, 313
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 4 cut(s) 75, 318, 393, 641
Tru9I TTAA 4 cut(s) 75, 318, 393, 641
TscAI CASTG 1 cut(s) 390
TseI GCWGC 4 cut(s) 111, 114, 173, 190
TspRI CASTG 1 cut(s) 390
VspI ATTAAT 2 cut(s) 393, 641
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.