RchiOBHm_Chr6g0297821

AUX/IAA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003240
Physical Location & Seq
Forward (+)
59466922 .. 59468587
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26733

Sequence Viewer

Length: 705 bp
ATGGAGCTTCAACTGGGTTTGGCTCTGTCGCTTCCGGTTCATCAGAACGCTCCTGTCAAAGGGTTTGATGTAACCAGTCATGGGGTGATGGGTTTGGATTTATGGAGCTATGGATGCTGCTTGGAGAGCAAAAAGAAGCGTAGTCACGACACGGCTTTCGAGAAGATTGGAAATGACGATGAGTCGAAGATGTTGCCTTTGTTGTTGTGGCATGGCCAGCCGGATGAGGAAGACGATGATGGCAAGAGGCAAAAGAAAAGAGACTCTTGCAGTCTCATCGATACCAATGATGATGGAGAAGGAAACCAAGTTGTTGGGTGGCCACCAATTAAATCATGGAGGAAGAAGATGTTTTTTCCTGATCATCAACACCATGGTCGTGGCCGCCACGATCTCCAGAATCATCAGAATATAGTGGCAAAGGAAAATGATGGGGCAGAAAATTCCATGTACGTAAAGGTGAAGATGGAAGGAGTGGGAATTCTGAGGAAGATAGATATAAAGCTGCATCACTCTTATCAGACACTTAGAGACTCCTTGATAGCCATGTTTGCCAAATGCAAAGGATGTGAGAATGAGGATACTGCAGATTTTACTTTGACTTATCAGGACGTACATGGAGATTGGTCGGTTGCTGGAGATGTTCCCTGGCAAACTTTCGTCAACTCCGTGCAGCGCCTGGAGATTGTAAAGAATGGTGGTTGA

Protein Analysis

234

Amino Acids

26.5

Weight (kDa)

5.93

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 30 - 232 3e-35 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 385
AcoI YGGCCR 3 cut(s) 214, 320, 382
AcsI RAATTY 2 cut(s) 442, 480
AfaI GTAC 2 cut(s) 452, 615
AfiI CCNNNNNNNGG 2 cut(s) 59, 81
AgsI TTSAA 1 cut(s) 11
AhdI GACNNNNNGTC 1 cut(s) 181
AjnI CCWGG 2 cut(s) 647, 678
AluBI AGCT 3 cut(s) 7, 108, 505
AluI AGCT 3 cut(s) 7, 108, 505
Alw26I GTCTC 3 cut(s) 255, 278, 525
AlwNI CAGNNNCTG 1 cut(s) 679
AoxI GGCC 3 cut(s) 214, 320, 382
ApeKI GCWGC 3 cut(s) 117, 505, 673
ApoI RAATTY 2 cut(s) 442, 480
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
AspLEI GCGC 1 cut(s) 678
AsuHPI GGTGA 2 cut(s) 97, 472
BalI TGGCCA 2 cut(s) 216, 322
BbsI GAAGAC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 104, 492, 685
BccI CCATC 5 cut(s) 82, 233, 287, 425, 460
BceAI ACGGC 1 cut(s) 168
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 259, 293
BciT130I CCWGG 2 cut(s) 649, 680
BciVI GTATCC 1 cut(s) 574
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 255, 278, 525
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 1 cut(s) 679
BfuI GTATCC 1 cut(s) 574
BisI GCNGC 4 cut(s) 118, 385, 506, 674
BlsI GCNGC 4 cut(s) 119, 386, 507, 675
Bme1390I CCNGG 2 cut(s) 649, 680
BmeRI GACNNNNNGTC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 649, 680
BmrI ACTGGG 1 cut(s) 23
BmsI GCATC 2 cut(s) 104, 517
BmuI ACTGGG 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 237
BpmI CTGGAG 3 cut(s) 380, 657, 701
Bsa29I ATCGAT 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 454
BsaJI CCNNGG 2 cut(s) 373, 647
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 81
Bse1I ACTGG 2 cut(s) 18, 75
BseBI CCWGG 2 cut(s) 649, 680
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 373, 647
BseGI GGATG 3 cut(s) 119, 229, 572
BseLI CCNNNNNNNGG 2 cut(s) 59, 81
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 18, 75
BseXI GCAGC 3 cut(s) 104, 492, 685
BsgI GTGCAG 1 cut(s) 692
BshFI GGCC 3 cut(s) 216, 322, 384
BshVI ATCGAT 1 cut(s) 279
BsiSI CCGG 2 cut(s) 35, 221
BslI CCNNNNNNNGG 2 cut(s) 59, 81
BsmAI GTCTC 3 cut(s) 255, 278, 525
BsnI GGCC 3 cut(s) 216, 322, 384
Bsp143I GATC 2 cut(s) 361, 391
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 1 cut(s) 385
BspANI GGCC 3 cut(s) 216, 322, 384
BspCNI CTCAG 1 cut(s) 477
BspDI ATCGAT 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 589
BsrI ACTGG 2 cut(s) 18, 75
BssECI CCNNGG 2 cut(s) 373, 647
BssMI GATC 2 cut(s) 361, 391
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 2 cut(s) 649, 680
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 2 cut(s) 485, 527
BstDSI CCRYGG 1 cut(s) 373
BstENI CCTNNNNNAGG 1 cut(s) 57
BstF5I GGATG 3 cut(s) 119, 229, 572
BstH2I RGCGCY 1 cut(s) 679
BstHHI GCGC 1 cut(s) 678
BstKTI GATC 2 cut(s) 364, 394
BstMAI GTCTC 3 cut(s) 255, 278, 525
BstMBI GATC 2 cut(s) 361, 391
BstMWI GCNNNNNNNGC 4 cut(s) 114, 126, 217, 551
BstNI CCWGG 2 cut(s) 649, 680
BstSCI CCNGG 2 cut(s) 647, 678
BstSFI CTRYAG 1 cut(s) 585
BstSNI TACGTA 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 104, 492, 685
BstV2I GAAGAC 1 cut(s) 237
BstXI CCANNNNNNTGG 2 cut(s) 314, 380
Bsu15I ATCGAT 1 cut(s) 279
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 3 cut(s) 216, 322, 384
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 3 cut(s) 119, 229, 572
Cac8I GCNNGC 1 cut(s) 218
CaiI CAGNNNCTG 1 cut(s) 679
CfoI GCGC 1 cut(s) 678
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 2 cut(s) 451, 614
CviAII CATG 7 cut(s) 80, 212, 336, 374, 448, 547, 617
CviQI GTAC 2 cut(s) 451, 614
DdeI CTNAG 2 cut(s) 485, 527
DpnI GATC 2 cut(s) 363, 393
DpnII GATC 2 cut(s) 361, 391
DriI GACNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 3 cut(s) 214, 320, 382
Eam1105I GACNNNNNGTC 1 cut(s) 181
Eco105I TACGTA 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 57
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 2 cut(s) 647, 678
EcoT14I CCWWGG 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 7 cut(s) 83, 215, 339, 377, 451, 550, 620
FalI AAGNNNNNCTT 2 cut(s) 250, 282
FatI CATG 7 cut(s) 79, 211, 335, 373, 447, 546, 616
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 4 cut(s) 118, 385, 506, 674
FokI GGATG 3 cut(s) 126, 236, 579
Fsp4HI GCNGC 4 cut(s) 118, 385, 506, 674
GlaI GCGC 1 cut(s) 677
GluI GCNGC 4 cut(s) 118, 385, 506, 674
GsuI CTGGAG 3 cut(s) 380, 657, 701
HaeII RGCGCY 1 cut(s) 679
HaeIII GGCC 3 cut(s) 216, 322, 384
HapII CCGG 2 cut(s) 35, 221
HhaI GCGC 1 cut(s) 678
Hin1II CATG 7 cut(s) 83, 215, 339, 377, 451, 550, 620
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 4 cut(s) 182, 263, 400, 533
HpaII CCGG 2 cut(s) 35, 221
HphI GGTGA 2 cut(s) 97, 472
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 4 cut(s) 45, 408, 486, 522
Hpy188III TCNNGA 5 cut(s) 146, 160, 359, 397, 608
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 2 cut(s) 293, 464
HpyCH4IV ACGT 2 cut(s) 453, 612
HpyCH4V TGCA 5 cut(s) 270, 508, 561, 587, 673
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 126, 217, 551
HpyF3I CTNAG 2 cut(s) 485, 527
HpySE526I ACGT 2 cut(s) 453, 612
Hsp92II CATG 7 cut(s) 83, 215, 339, 377, 451, 550, 620
HspAI GCGC 1 cut(s) 676
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 2 cut(s) 361, 391
LmnI GCTCC 3 cut(s) 4, 55, 105
Lsp1109I GCAGC 3 cut(s) 104, 492, 685
LweI GCATC 2 cut(s) 104, 517
MaeII ACGT 2 cut(s) 453, 612
MaeIII GTNAC 2 cut(s) 70, 143
MalI GATC 2 cut(s) 363, 393
MboI GATC 2 cut(s) 361, 391
MboII GAAGA 7 cut(s) 175, 199, 242, 355, 358, 475, 502
MlsI TGGCCA 2 cut(s) 216, 322
MluCI AATT 3 cut(s) 327, 442, 480
MluNI TGGCCA 2 cut(s) 216, 322
MlyI GAGTC 3 cut(s) 191, 257, 527
MnlI CCTC 5 cut(s) 220, 240, 333, 480, 571
Mox20I TGGCCA 2 cut(s) 216, 322
MscI TGGCCA 2 cut(s) 216, 322
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 2 cut(s) 216, 322
MspI CCGG 2 cut(s) 35, 221
MspR9I CCNGG 2 cut(s) 649, 680
MvaI CCWGG 2 cut(s) 649, 680
MwoI GCNNNNNNNGC 4 cut(s) 114, 126, 217, 551
NcoI CCATGG 1 cut(s) 373
NdeII GATC 2 cut(s) 361, 391
NlaIII CATG 7 cut(s) 83, 215, 339, 377, 451, 550, 620
NmuCI GTSAC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 666
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 4 cut(s) 119, 386, 507, 675
PleI GAGTC 3 cut(s) 190, 257, 527
PpsI GAGTC 3 cut(s) 190, 257, 527
Ppu21I YACGTR 1 cut(s) 454
Psp6I CCWGG 2 cut(s) 647, 678
PspGI CCWGG 2 cut(s) 647, 678
PstI CTGCAG 1 cut(s) 589
PstNI CAGNNNCTG 1 cut(s) 679
RsaI GTAC 2 cut(s) 452, 615
RsaNI GTAC 2 cut(s) 451, 614
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 4 cut(s) 118, 385, 506, 674
Sau3AI GATC 2 cut(s) 361, 391
SchI GAGTC 3 cut(s) 191, 257, 527
ScrFI CCNGG 2 cut(s) 649, 680
SetI ASST 6 cut(s) 9, 110, 456, 462, 507, 615
SfaNI GCATC 2 cut(s) 104, 517
SfcI CTRYAG 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 454
Sse9I AATT 3 cut(s) 327, 442, 480
SsiI CCGC 1 cut(s) 385
StyD4I CCNGG 2 cut(s) 647, 678
StyI CCWWGG 1 cut(s) 373
TaiI ACGT 2 cut(s) 456, 615
TaqI TCGA 3 cut(s) 159, 185, 279
TasI AATT 3 cut(s) 327, 442, 480
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 3 cut(s) 117, 505, 673
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 1 cut(s) 29
TspGWI ACGGA 1 cut(s) 658
XagI CCTNNNNNAGG 1 cut(s) 57
XapI RAATTY 2 cut(s) 442, 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.