MD09G1033400.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005928
Physical Location & Seq
Reverse (-)
2051431 .. 2051673
243 bp
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UTR
Exon/CDS
Intron
MD09G1033400.v1.1.491

Sequence Viewer

Length: 243 bp
ATGGTGAAGGCAGGCGGGAAGGTACTGACAATTGTACGTCCAGTAGCGCCACCGGCATTCCTATTTATGCTGACTTCAACCGGAACTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAGGCCGGTGCTTGATCCCACCGGGCCGTATCCATTTTCGAAGAATGTTGAAGCATTTGCCTATCTTGAAACCTCCAGAGCTACCGGAAAGGTGGTTGTGTATCCCATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

81

Amino Acids

8.65

Weight (kDa)

9.75

Isoelectric Point (pI)

22.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N_2 PF13602 20 - 76 1.2e-11 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 15
AclWI GGATC 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 122
AfaI GTAC 2 cut(s) 24, 36
AgsI TTSAA 3 cut(s) 78, 179, 197
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
AlwI GGATC 1 cut(s) 137
AoxI GGCC 2 cut(s) 131, 152
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 2 cut(s) 16, 139
AsuII TTCGAA 1 cut(s) 167
BceAI ACGGC 1 cut(s) 139
BciVI GTATCC 2 cut(s) 168, 240
BcnI CCSGG 1 cut(s) 151
BfoI RGCGCY 1 cut(s) 50
BfuI GTATCC 2 cut(s) 168, 240
Bme1390I CCNGG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 152
BmrFI CCNGG 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 187
Bpu14I TTCGAA 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 151
BsaWI WCCGGW 2 cut(s) 80, 212
Bse118I RCCGGY 2 cut(s) 52, 133
Bse1I ACTGG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 41
BshFI GGCC 2 cut(s) 133, 154
BsiSI CCGG 5 cut(s) 53, 81, 134, 150, 213
BsmI GAATGC 1 cut(s) 56
BsnI GGCC 2 cut(s) 133, 154
Bsp119I TTCGAA 1 cut(s) 167
Bsp143I GATC 1 cut(s) 142
BspACI CCGC 1 cut(s) 15
BspANI GGCC 2 cut(s) 133, 154
BspPI GGATC 1 cut(s) 137
BspT104I TTCGAA 1 cut(s) 167
BsrFI RCCGGY 2 cut(s) 52, 133
BsrI ACTGG 1 cut(s) 41
BssAI RCCGGY 2 cut(s) 52, 133
BssMI GATC 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 149
BsuI GTATCC 2 cut(s) 168, 240
BsuRI GGCC 2 cut(s) 133, 154
Cac8I GCNNGC 1 cut(s) 13
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 2 cut(s) 52, 133
Cfr13I GGNCC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 23, 35
CviAII CATG 1 cut(s) 240
CviJI RGCY 4 cut(s) 106, 133, 154, 209
CviKI_1 RGCY 4 cut(s) 106, 133, 154, 209
CviQI GTAC 2 cut(s) 23, 35
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 122
FaeI CATG 1 cut(s) 243
FaiI YATR 2 cut(s) 68, 241
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 1 cut(s) 239
FauI CCCGC 1 cut(s) 8
GlaI GCGC 1 cut(s) 48
GsuI CTGGAG 1 cut(s) 187
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 2 cut(s) 133, 154
HapII CCGG 5 cut(s) 53, 81, 134, 150, 213
HhaI GCGC 1 cut(s) 49
Hin1II CATG 1 cut(s) 243
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HpaII CCGG 5 cut(s) 53, 81, 134, 150, 213
HphI GGTGA 2 cut(s) 16, 139
Hpy188III TCNNGA 2 cut(s) 194, 204
HpyAV CCTTC 2 cut(s) 13, 118
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 1 cut(s) 243
HspAI GCGC 1 cut(s) 47
Kzo9I GATC 1 cut(s) 142
LpnPI CCDG 7 cut(s) 54, 66, 94, 147, 163, 217, 226
MaeII ACGT 1 cut(s) 37
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 1 cut(s) 181
MfeI CAATTG 1 cut(s) 30
MluCI AATT 1 cut(s) 30
MnlI CCTC 2 cut(s) 123, 211
MspI CCGG 5 cut(s) 53, 81, 134, 150, 213
MspR9I CCNGG 1 cut(s) 151
MunI CAATTG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 53
NciI CCSGG 1 cut(s) 151
NdeII GATC 1 cut(s) 142
NlaIII CATG 1 cut(s) 243
NspV TTCGAA 1 cut(s) 167
PctI GAATGC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 152
RsaI GTAC 2 cut(s) 24, 36
RsaNI GTAC 2 cut(s) 23, 35
Sau3AI GATC 1 cut(s) 142
Sau96I GGNCC 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 151
SetI ASST 6 cut(s) 24, 40, 129, 203, 211, 222
SfuI TTCGAA 1 cut(s) 167
Sse9I AATT 1 cut(s) 30
SsiI CCGC 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 40
TaqI TCGA 1 cut(s) 167
TasI AATT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.