Rh2DG656500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005928
Physical Location & Seq
Reverse (-)
88133951 .. 88134277
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG656500.1

Sequence Viewer

Length: 327 bp
ATGAAAGTGAACTTTCATGTGATCTGGGATGGTCCGAGAAGAGGTGCCGGAGTGGCGGCCATTGTCCGGAACCACTGGGGAGAGATGATGGATGGCGTCTCTGCGTTCGTTCAGACAAATTCGAAGCTCGAGGCAGAGGCCAAGGCTGCAGCCTTGGTCTCCAGGCTTGCCCAGACTCAAGGTTCCTACAAGATCATCTTTCAGTGTTCGAGTCCATTTCTGGTGAGATCTATTTCCACGGTTTCTTCCCTCCTTGACAGCTGGGTGTTCAAGGCAGATTTTCTTGAAGCGATCGACAATTGCAAAAATCTGGAGGGATTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RVT_1 zf-RVT

Protein Analysis

108

Amino Acids

11.91

Weight (kDa)

8.69

Isoelectric Point (pI)

16.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 6 - 82 1.5e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AccIII TCCGGA 1 cut(s) 66
AciI CCGC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 96
AfiI CCNNNNNNNGG 2 cut(s) 41, 66
AgsI TTSAA 2 cut(s) 271, 287
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 2 cut(s) 127, 261
AluI AGCT 2 cut(s) 127, 261
Alw26I GTCTC 2 cut(s) 103, 163
Ama87I CYCGRG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 66
AoxI GGCC 2 cut(s) 57, 138
ApeKI GCWGC 2 cut(s) 146, 149
ApoI RAATTY 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 235
AsuII TTCGAA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 32
BanI GGYRCC 1 cut(s) 44
BbvI GCAGC 2 cut(s) 133, 161
BccI CCATC 3 cut(s) 23, 82, 86
BciT130I CCWGG 1 cut(s) 163
BcoDI GTCTC 2 cut(s) 103, 163
BfmI CTRYAG 1 cut(s) 147
BglI GCCNNNNNGGC 1 cut(s) 53
BglII AGATCT 1 cut(s) 227
BisI GCNGC 3 cut(s) 57, 147, 150
BlsI GCNGC 3 cut(s) 58, 148, 151
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 32
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 3 cut(s) 46, 71, 184
BmrFI CCNGG 1 cut(s) 163
BmrI ACTGGG 1 cut(s) 85
BmuI ACTGGG 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 3 cut(s) 141, 153, 237
BsaWI WCCGGW 1 cut(s) 66
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 66
Bse1I ACTGG 1 cut(s) 80
BseAI TCCGGA 1 cut(s) 66
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 3 cut(s) 141, 153, 237
BseGI GGATG 2 cut(s) 34, 97
BseLI CCNNNNNNNGG 2 cut(s) 41, 66
BseNI ACTGG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 133, 161
BseYI CCCAGC 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 2 cut(s) 59, 140
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 48, 67
BslI CCNNNNNNNGG 2 cut(s) 41, 66
BsmAI GTCTC 2 cut(s) 103, 163
BsmBI CGTCTC 1 cut(s) 103
BsnI GGCC 2 cut(s) 59, 140
Bso31I GGTCTC 1 cut(s) 163
BsoBI CYCGRG 1 cut(s) 128
Bsp119I TTCGAA 1 cut(s) 122
Bsp13I TCCGGA 1 cut(s) 66
Bsp143I GATC 4 cut(s) 21, 192, 227, 291
BspACI CCGC 1 cut(s) 56
BspANI GGCC 2 cut(s) 59, 140
BspEI TCCGGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 46, 71, 184
BspMAI CTGCAG 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 163
BsrI ACTGG 1 cut(s) 80
BssECI CCNNGG 3 cut(s) 141, 153, 237
BssMI GATC 4 cut(s) 21, 192, 227, 291
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 141, 153
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 168
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 34, 97
BstKTI GATC 4 cut(s) 24, 195, 230, 294
BstMAI GTCTC 2 cut(s) 103, 163
BstMBI GATC 4 cut(s) 21, 192, 227, 291
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 2 cut(s) 53, 146
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 133, 161
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuRI GGCC 2 cut(s) 59, 140
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 34, 97
BtsIMutI CAGTG 2 cut(s) 73, 209
Cac8I GCNNGC 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 1 cut(s) 85
CviAII CATG 1 cut(s) 17
CviJI RGCY 7 cut(s) 59, 127, 140, 146, 152, 166, 261
CviKI_1 RGCY 7 cut(s) 59, 127, 140, 146, 152, 166, 261
DpnI GATC 4 cut(s) 23, 194, 229, 293
DpnII GATC 4 cut(s) 21, 192, 227, 291
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 141, 153
Eco31I GGTCTC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 141, 153
ErhI CCWWGG 2 cut(s) 141, 153
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 1 cut(s) 20
FaiI YATR 1 cut(s) 18
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 1 cut(s) 16
Fnu4HI GCNGC 3 cut(s) 57, 147, 150
FokI GGATG 2 cut(s) 41, 104
Fsp4HI GCNGC 3 cut(s) 57, 147, 150
GluI GCNGC 3 cut(s) 57, 147, 150
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 2 cut(s) 59, 140
HapII CCGG 2 cut(s) 48, 67
HgaI GACGC 1 cut(s) 85
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 1 cut(s) 20
HinfI GANTC 2 cut(s) 175, 211
HpaII CCGG 2 cut(s) 48, 67
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 2 cut(s) 36, 114
Hpy188III TCNNGA 3 cut(s) 67, 284, 311
Hpy8I GTNNAC 1 cut(s) 10
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 2 cut(s) 149, 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 146
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 20
Kpn2I TCCGGA 1 cut(s) 66
Kzo9I GATC 4 cut(s) 21, 192, 227, 291
Lsp1109I GCAGC 2 cut(s) 133, 161
MalI GATC 4 cut(s) 23, 194, 229, 293
MboI GATC 4 cut(s) 21, 192, 227, 291
MboII GAAGA 2 cut(s) 51, 237
MfeI CAATTG 1 cut(s) 298
MflI RGATCY 1 cut(s) 227
MluCI AATT 2 cut(s) 118, 298
MlyI GAGTC 2 cut(s) 169, 220
MnlI CCTC 5 cut(s) 35, 124, 130, 260, 307
MroI TCCGGA 1 cut(s) 66
MseI TTAA 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 261
MspI CCGG 2 cut(s) 48, 67
MspR9I CCNGG 1 cut(s) 163
MunI CAATTG 1 cut(s) 298
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 2 cut(s) 53, 146
NdeII GATC 4 cut(s) 21, 192, 227, 291
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 3 cut(s) 46, 71, 184
NspV TTCGAA 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 128
PkrI GCNGC 3 cut(s) 58, 148, 151
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 2 cut(s) 169, 219
PpsI GAGTC 2 cut(s) 169, 219
Psp6I CCWGG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 3 cut(s) 46, 71, 184
PspPI GGNCC 1 cut(s) 32
PspXI VCTCGAGB 1 cut(s) 128
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 227
PvuI CGATCG 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 261
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 57, 147, 150
Sau3AI GATC 4 cut(s) 21, 192, 227, 291
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 2 cut(s) 169, 220
ScrFI CCNGG 1 cut(s) 163
SetI ASST 4 cut(s) 46, 129, 184, 263
SfcI CTRYAG 1 cut(s) 147
Sfr274I CTCGAG 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 32
SlaI CTCGAG 1 cut(s) 128
SmlI CTYRAG 2 cut(s) 128, 177
SmoI CTYRAG 2 cut(s) 128, 177
Sse9I AATT 2 cut(s) 118, 298
SsiI CCGC 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 2 cut(s) 141, 153
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 4 cut(s) 122, 129, 209, 294
TasI AATT 2 cut(s) 118, 298
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 80, 209
TseI GCWGC 2 cut(s) 146, 149
TspDTI ATGAA 2 cut(s) 5, 17
TspRI CASTG 2 cut(s) 80, 209
VpaK11BI GGWCC 1 cut(s) 32
XapI RAATTY 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.