Rh2AG629400

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005928
Physical Location & Seq
Reverse (-)
85439655 .. 85441537
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG629400.1

Sequence Viewer

Length: 423 bp
ATGGCTCTGCAGAAGGTGAACCAATCTTATGTTATTGGAACTTCAACCAAGGTTGACAGCTCTGGTGTTAACATGGAGAAGTTTGATGACAAGTACTTTGCCAAGGAGGGTCAAAAGAAGAAAAAGAAGGGAGAAGGCAAGTTCTTTGAAGTGGAGAAGGAGACTGACAAGACGGTGAAGGTGGTGAGGGAAGGTAGAAAGGTGGTCACAATAGTAGGTCCAGTAATGCCTCCGGCGTTCAGATTTGTGCTCACCCCTACAGGTTCTATCTTGGAGAAACTGAAGCCGTACTCGGGGAGCAGGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCACTTTTATAAAGTTGTTGAAGCACTTGGTTACCTTGAGACTTCAAGAGCTACAGGAAAGGTTGTTGTGTACCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

140

Amino Acids

15.62

Weight (kDa)

9.74

Isoelectric Point (pI)

18.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e PF01159 3 - 63 1.4e-14 Ribosomal protein L6e
ADH_zinc_N_2 PF13602 56 - 136 5.1e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AclWI GGATC 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 3 cut(s) 95, 290, 410
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 4 cut(s) 45, 149, 359, 384
AluBI AGCT 2 cut(s) 60, 389
AluI AGCT 2 cut(s) 60, 389
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 155, 371
AlwI GGATC 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AspS9I GGNCC 2 cut(s) 218, 332
AsuHPI GGTGA 5 cut(s) 28, 187, 196, 244, 319
AvaI CYCGRG 1 cut(s) 292
AvaII GGWCC 2 cut(s) 218, 332
Bbv12I GWGCWC 1 cut(s) 252
BccI CCATC 1 cut(s) 422
BceAI ACGGC 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 155, 371
BfmI CTRYAG 3 cut(s) 8, 258, 390
BmcAI AGTACT 1 cut(s) 95
Bme18I GGWCC 2 cut(s) 218, 332
BmeT110I CYCGRG 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 218, 332
BpuEI CTTGAG 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 48, 102
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 221, 314
BseDI CCNNGG 2 cut(s) 48, 102
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 293
BseNI ACTGG 2 cut(s) 221, 314
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 292
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 2 cut(s) 155, 371
BsoBI CYCGRG 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 322
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 1 cut(s) 317
BsrI ACTGG 2 cut(s) 221, 314
BssECI CCNNGG 2 cut(s) 48, 102
BssMI GATC 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 48, 102
Bst4CI ACNGT 1 cut(s) 175
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 1 cut(s) 414
BstKTI GATC 1 cut(s) 325
BstMAI GTCTC 2 cut(s) 155, 371
BstMBI GATC 1 cut(s) 322
BstPI GGTNACC 1 cut(s) 368
BstSFI CTRYAG 3 cut(s) 8, 258, 390
BtsCI GGATG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 321
Cfr13I GGNCC 2 cut(s) 218, 332
Csp6I GTAC 3 cut(s) 94, 289, 409
CviAII CATG 2 cut(s) 73, 420
CviJI RGCY 5 cut(s) 5, 60, 286, 313, 389
CviKI_1 RGCY 5 cut(s) 5, 60, 286, 313, 389
CviQI GTAC 3 cut(s) 94, 289, 409
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 48, 102
Eco47I GGWCC 2 cut(s) 218, 332
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 292
Eco91I GGTNACC 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 368
EcoT14I CCWWGG 2 cut(s) 48, 102
ErhI CCWWGG 2 cut(s) 48, 102
FaeI CATG 2 cut(s) 76, 423
FaiI YATR 5 cut(s) 30, 74, 337, 348, 421
FatI CATG 2 cut(s) 72, 419
FokI GGATG 1 cut(s) 401
HapII CCGG 1 cut(s) 233
Hin1II CATG 2 cut(s) 76, 423
HincII GTYRAC 2 cut(s) 55, 70
HindII GTYRAC 2 cut(s) 55, 70
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 1 cut(s) 233
HphI GGTGA 5 cut(s) 28, 187, 196, 244, 319
Hpy166II GTNNAC 4 cut(s) 19, 55, 70, 409
Hpy188I TCNGA 1 cut(s) 242
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 4 cut(s) 19, 55, 70, 409
HpyAV CCTTC 7 cut(s) 7, 121, 128, 151, 172, 185, 298
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4V TGCA 1 cut(s) 10
Hsp92II CATG 2 cut(s) 76, 423
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 8 cut(s) 48, 234, 246, 246, 286, 315, 327, 378
MaeIII GTNAC 2 cut(s) 205, 368
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 252
MnlI CCTC 3 cut(s) 100, 180, 240
MseI TTAA 1 cut(s) 69
MspI CCGG 1 cut(s) 233
NdeII GATC 1 cut(s) 322
NlaIII CATG 2 cut(s) 76, 423
NmuCI GTSAC 1 cut(s) 205
PsiI TTATAA 1 cut(s) 348
PspEI GGTNACC 1 cut(s) 368
PspPI GGNCC 2 cut(s) 218, 332
PstI CTGCAG 1 cut(s) 12
RsaI GTAC 3 cut(s) 95, 290, 410
RsaNI GTAC 3 cut(s) 94, 289, 409
SaqAI TTAA 1 cut(s) 69
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 2 cut(s) 218, 332
ScaI AGTACT 1 cut(s) 95
SduI GDGCHC 1 cut(s) 252
SfcI CTRYAG 3 cut(s) 8, 258, 390
SinI GGWCC 2 cut(s) 218, 332
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
StyI CCWWGG 2 cut(s) 48, 102
TaaI ACNGT 1 cut(s) 175
TatI WGTACW 1 cut(s) 93
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 321
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspRI CASTG 1 cut(s) 321
VpaK11BI GGWCC 2 cut(s) 218, 332
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.