pycom111g02660

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0005928
Physical Location & Seq
Reverse (-)
1605048 .. 1605761
714 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02660.1

Sequence Viewer

Length: 561 bp
ATGAAGGGCTTGAACGAGTTGAATTCTCTGCCGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGGAGTCGGAACACATGTTATTCAGGCAGCTAGCAAAGCATGTGTTTGGTGCTTCAAAAGTAGCAGCTACAGCGAGCACCAAAAAACTCGATCTTTTGAGAAGCTTGGGTGCTGATTTGGCTATTGATTACACCAAGGAGAACTTCGAAGACCTGCCCGAGAAATTCGATGTGGTTTATGATGCAGTTGGTATAATTCTTGAATCTTCATCGTTATTTTTTTCTTATACAAATCAAAGTGACAGGGCATTGAAGGCGGTGAAGGAAGGCGGGAAGGTTGTGACAATCGTAGGTCCAGTAACGCCACCGGCAATCATATTTATACTGACTTCAACCGGAACTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAGGCTGGTGCTTGATCCCACCAGCCCGTATCCATTTTCGAAGACTGTTGAAGCATTTGCCTATCTTGAAACCTCCAGAGCTACCGGAAAGGTGATTGTGTATCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

187

Amino Acids

20.36

Weight (kDa)

6.15

Isoelectric Point (pI)

25.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 31 - 117 9.5e-09 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 57 - 182 9e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 225
AciI CCGC 2 cut(s) 320, 333
AclWI GGATC 1 cut(s) 455
AcsI RAATTY 2 cut(s) 22, 227
AcuI CTGAAG 1 cut(s) 440
AfiI CCNNNNNNNGG 1 cut(s) 51
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 8 cut(s) 13, 22, 120, 266, 316, 396, 497, 515
AjuI GAANNNNNNNTTGG 4 cut(s) 191, 223, 413, 445
AluBI AGCT 4 cut(s) 94, 131, 168, 527
AluI AGCT 4 cut(s) 94, 131, 168, 527
Alw21I GWGCWC 1 cut(s) 143
AlwI GGATC 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 221
ApeKI GCWGC 2 cut(s) 91, 128
ApoI RAATTY 2 cut(s) 22, 227
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 3 cut(s) 334, 457, 550
AsuII TTCGAA 2 cut(s) 210, 485
AsuNHI GCTAGC 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 356
BbsI GAAGAC 2 cut(s) 219, 494
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 103, 140
BccI CCATC 2 cut(s) 49, 560
BciVI GTATCC 2 cut(s) 486, 558
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 225
BfuI GTATCC 2 cut(s) 486, 558
BisI GCNGC 2 cut(s) 92, 129
BlsI GCNGC 2 cut(s) 93, 130
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 356
BmsI GCATC 1 cut(s) 235
BmtI GCTAGC 1 cut(s) 98
BpiI GAAGAC 2 cut(s) 219, 494
BpmI CTGGAG 1 cut(s) 505
Bpu14I TTCGAA 2 cut(s) 210, 485
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 2 cut(s) 398, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse118I RCCGGY 2 cut(s) 31, 370
Bse1I ACTGG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 2 cut(s) 41, 552
BseLI CCNNNNNNNGG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 359
BseXI GCAGC 2 cut(s) 103, 140
BseYI CCCAGC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 4 cut(s) 32, 371, 399, 531
BslI CCNNNNNNNGG 1 cut(s) 51
BsoBI CYCGRG 1 cut(s) 221
Bsp119I TTCGAA 2 cut(s) 210, 485
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 154, 460
BspACI CCGC 2 cut(s) 320, 333
BspMI ACCTGC 1 cut(s) 225
BspOI GCTAGC 1 cut(s) 98
BspPI GGATC 1 cut(s) 455
BspT104I TTCGAA 2 cut(s) 210, 485
BsrFI RCCGGY 2 cut(s) 31, 370
BsrI ACTGG 1 cut(s) 359
BssAI RCCGGY 2 cut(s) 31, 370
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 2 cut(s) 154, 460
BssT1I CCWWGG 1 cut(s) 198
Bst4CI ACNGT 2 cut(s) 406, 493
BstBI TTCGAA 2 cut(s) 210, 485
BstC8I GCNNGC 2 cut(s) 96, 139
BstF5I GGATG 2 cut(s) 41, 552
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 2 cut(s) 157, 463
BstMBI GATC 2 cut(s) 154, 460
BstMWI GCNNNNNNNGC 4 cut(s) 100, 134, 182, 317
BstNSI RCATGY 2 cut(s) 82, 107
BstSFI CTRYAG 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 103, 140
BstV2I GAAGAC 2 cut(s) 219, 494
BsuI GTATCC 2 cut(s) 486, 558
BtsCI GGATG 2 cut(s) 41, 552
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 2 cut(s) 96, 139
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 2 cut(s) 31, 370
Cfr13I GGNCC 1 cut(s) 356
CviAII CATG 3 cut(s) 79, 104, 558
CviJI RGCY 9 cut(s) 9, 94, 131, 168, 185, 424, 451, 471, 527
CviKI_1 RGCY 9 cut(s) 9, 94, 131, 168, 185, 424, 451, 471, 527
DpnI GATC 2 cut(s) 156, 462
DpnII GATC 2 cut(s) 154, 460
Eco130I CCWWGG 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 3 cut(s) 82, 107, 561
FaiI YATR 8 cut(s) 80, 105, 243, 257, 291, 380, 386, 559
FalI AAGNNNNNCTT 4 cut(s) 191, 223, 413, 445
FatI CATG 3 cut(s) 78, 103, 557
FauI CCCGC 1 cut(s) 326
Fnu4HI GCNGC 2 cut(s) 92, 129
FokI GGATG 2 cut(s) 28, 539
Fsp4HI GCNGC 2 cut(s) 92, 129
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 60
GluI GCNGC 2 cut(s) 92, 129
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 505
HaeII RGCGCY 1 cut(s) 62
HapII CCGG 4 cut(s) 32, 371, 399, 531
HhaI GCGC 1 cut(s) 61
Hin1II CATG 3 cut(s) 82, 107, 561
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 2 cut(s) 68, 266
HpaII CCGG 4 cut(s) 32, 371, 399, 531
HphI GGTGA 3 cut(s) 334, 457, 550
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 3 cut(s) 263, 512, 522
HpyAV CCTTC 5 cut(s) 310, 319, 323, 331, 436
HpyCH4III ACNGT 2 cut(s) 406, 493
HpyCH4V TGCA 1 cut(s) 248
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 134, 182, 317
Hsp92II CATG 3 cut(s) 82, 107, 561
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 2 cut(s) 154, 460
Lsp1109I GCAGC 2 cut(s) 103, 140
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 3 cut(s) 302, 343, 361
MalI GATC 2 cut(s) 156, 462
MboI GATC 2 cut(s) 154, 460
MboII GAAGA 3 cut(s) 224, 261, 499
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 3 cut(s) 22, 227, 258
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 3 cut(s) 49, 441, 529
MspI CCGG 4 cut(s) 32, 371, 399, 531
MwoI GCNNNNNNNGC 4 cut(s) 100, 134, 182, 317
NdeII GATC 2 cut(s) 154, 460
NheI GCTAGC 1 cut(s) 94
NlaIII CATG 3 cut(s) 82, 107, 561
NmuCI GTSAC 2 cut(s) 302, 343
NspI RCATGY 2 cut(s) 82, 107
NspV TTCGAA 2 cut(s) 210, 485
PciI ACATGT 1 cut(s) 78
PfeI GAWTC 1 cut(s) 266
PkrI GCNGC 2 cut(s) 93, 130
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
PscI ACATGT 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 61
PspPI GGNCC 1 cut(s) 356
SatI GCNGC 2 cut(s) 92, 129
Sau3AI GATC 2 cut(s) 154, 460
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 132
SfuI TTCGAA 2 cut(s) 210, 485
SinI GGWCC 1 cut(s) 356
Sse9I AATT 3 cut(s) 22, 227, 258
SsiI CCGC 2 cut(s) 320, 333
SspMI CTAG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 2 cut(s) 406, 493
TaqI TCGA 4 cut(s) 153, 210, 231, 485
TasI AATT 3 cut(s) 22, 227, 258
TfiI GAWTC 1 cut(s) 266
TseFI GTSAC 2 cut(s) 302, 343
TseI GCWGC 2 cut(s) 91, 128
Tsp45I GTSAC 2 cut(s) 302, 343
TspDTI ATGAA 2 cut(s) 17, 261
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 2 cut(s) 22, 227
XceI RCATGY 2 cut(s) 82, 107
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.