Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Reverse (-)

2772643 .. 2774190

Gene Structure 1548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1043100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGCCACCGGTATGAAGGAAGGTTCTCTTCCTACCTTGCCGGCAATCGACCGCTGCCCTTCAATCGGTCGCGAAAAACACACTGTCGTGGCGGACATGGACGGCACTTTGCTAAGAGGACGGAGCTCCTTCCCTTACTTTGCCCTCGTCGCCTTTGAGGCCGGCGGCATACTAAGGCTTCTCTTTTTACTACTGGCGTCTCCACTCATTGGTCTGCTGTACTACTTTGTCTCGGAGTCAGCCGGCATTCGTGTCATGGTTTTCGCCACTTTCGCCGGTATGAGAGTCTCCGACATAGAATCTGTGGCGCGTGCGATCCTCCCCAAGTTCTATTCCGCCGACTTGCACCCCGAGACGTGGAGAGTCTTTTCTTCGTGTGGGAAACGCTGCGTTCTGACCGCAAACCCGAGGATAATGGTGGAGCCGTTTTTGAAGGAGTTTTTGGGCGCTGACATGGTTCTCGGGACTGAGATCGCCACCTACAAAAGCAGAGCCACAGGTTGGGTCCTCAGTCCTGGGGTTCTGGTCGACGGAGGCAAGGCCAACGCGCTTAACAAAGCGTTCGGCACTGACCCCTCATCAGCTCCTGATGTCGGGCTTGGCGACCGAAAAACGGACTACCCATTCATGAAGTTATGCAAGGAAAGCTATGTGGTTCCTGCTACGCCGGAAGTTGCGGCGGTGAGCCTCGACAAGTTGCCAAAACCGATAGTATTTCACGACGGAAGGCTGGTTCAGAAGCCATCGCCGCTCTTGGCGTTGCTTATAATTCTTTGGATCCCGGTCGGTTTTACTTTAGCTTGCTTGCGAATGGCAGCGGGCGCACTGCTTCCTATGCCCGTCGTATACTACGCTTTATGGGCGTTGGGCGTACGGGTGTACATCAAAGGAACCCCACCGCCTCCCGCCAAGAAATCAACAGGCCAGACCGGAGTCCTTTTCATTTGCTCACACCGCACTCTCCTCGACCCGATTTTTCTCTCAATCGCTCTGGGCAGGCCCATCCCGGCCGTGACCTACTCTCTCTCCCGTCTCTCCGAACTCATCTCGCCAATTAAAACCGTCCGACTCACACGGGACCGGGTCAAGGACGCGAACCAGATTAAAGACCTCTTAGAACAAGGCGATCTCGTCATATGCCCCGAGGGAACAACGTGCCGAGAACCATTTTTGCTACGATTCTCCGCCCTGTTTGCAGAGCTGACCAACGAGCTCGTCCCTGTAGCCATGTCAAACAGCATGAGCATGTTCCACGGGACGACGGCCCGAGGGTGGAAGGGGATGGACCCGTTCTACTTCTTCATGAACCCTAGCCCGGCGTACGAAGTAACGTTTTTAAACAAGATGCCGTACGAGCTGACTTGTGGAGCCGGAAAGTCGAGCTACGACGTGGCGAATTGCATGCAGAGGACCATCGCTGCGAGCCTGTCGTATGAGTGCACCACCTTCACGCGGAAGGACAAGTACCGGGCATTGGCGGGGAATGACGGCATCGTCGAGGAAAGGTCTAAGCGTACGGCTGCAGCCAATAAAATTATGGGCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

516

Amino Acids

56.25

Weight (kDa)

9.12

Isoelectric Point (pI)

43.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	28 - 219	6e-64	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	29 - 211	9.1e-27	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	309 - 410	8e-07	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	767
AasI	GACNNNNNNGTC	1 cut(s)	1493
AccB7I	CCANNNNNTGG	2 cut(s)	209, 501
AccBSI	CCGCTC	1 cut(s)	751
AccI	GTMKAC	2 cut(s)	528, 846
AccII	CGCG	5 cut(s)	72, 310, 548, 1094, 1453
AclI	AACGTT	1 cut(s)	1331
AclWI	GGATC	3 cut(s)	310, 772, 785
AcoI	YGGCCR	2 cut(s)	3, 1008
AcyI	GRCGYC	1 cut(s)	197
AfaI	GTAC	7 cut(s)	221, 873, 881, 1322, 1352, 1466, 1516
AgeI	ACCGGT	1 cut(s)	8
AgsI	TTSAA	2 cut(s)	63, 433
AjiI	CACGTC	2 cut(s)	357, 1390
AjnI	CCWGG	1 cut(s)	514
AjuI	GAANNNNNNNTTGG	2 cut(s)	425, 457
AleI	CACNNNNGTG	1 cut(s)	86
AloI	GAACNNNNNNTCC	2 cut(s)	717, 749
AluBI	AGCT	8 cut(s)	126, 584, 648, 800, 1201, 1213, 1357, 1383
AluI	AGCT	8 cut(s)	126, 584, 648, 800, 1201, 1213, 1357, 1383
Alw21I	GWGCWC	3 cut(s)	128, 1215, 1442
Alw26I	GTCTC	5 cut(s)	204, 235, 292, 347, 1037
Alw44I	GTGCAC	1 cut(s)	1438
AlwI	GGATC	3 cut(s)	310, 772, 785
AlwNI	CAGNNNCTG	1 cut(s)	587
Ama87I	CYCGRG	5 cut(s)	350, 406, 461, 1142, 1266
AoxI	GGCC	7 cut(s)	3, 159, 540, 922, 998, 1008, 1263
ApaLI	GTGCAC	1 cut(s)	1438
ApeKI	GCWGC	7 cut(s)	54, 387, 815, 1418, 1520, 1523, 1542
AsiGI	ACCGGT	1 cut(s)	8
AspLEI	GCGC	4 cut(s)	310, 449, 550, 824
AspS9I	GGNCC	6 cut(s)	505, 999, 1078, 1264, 1285, 1410
AsuC2I	CCSGG	5 cut(s)	782, 1007, 1082, 1316, 1469
AsuHPI	GGTGA	1 cut(s)	694
AvaI	CYCGRG	5 cut(s)	350, 406, 461, 1142, 1266
AvaII	GGWCC	4 cut(s)	505, 1078, 1285, 1410
BaeGI	GKGCMC	1 cut(s)	1442
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	777
BanII	GRGCYC	2 cut(s)	128, 1215
BarI	GAAGNNNNNNTAC	2 cut(s)	1448, 1480
Bbv12I	GWGCWC	3 cut(s)	128, 1215, 1442
BbvI	GCAGC	7 cut(s)	41, 374, 827, 1405, 1507, 1529, 1535
BccI	CCATC	4 cut(s)	751, 1010, 1276, 1421
BceAI	ACGGC	7 cut(s)	118, 409, 995, 1278, 1333, 1504, 1533
BcgI	CGANNNNNNTGC	4 cut(s)	946, 980, 1384, 1418
BciT130I	CCWGG	1 cut(s)	516
BcnI	CCSGG	5 cut(s)	782, 1007, 1082, 1316, 1469
BcoDI	GTCTC	5 cut(s)	204, 235, 292, 347, 1037
BfaI	CTAG	2 cut(s)	1311, 1546
BfmI	CTRYAG	2 cut(s)	1221, 1521
BfoI	RGCGCY	1 cut(s)	450
BglI	GCCNNNNNGGC	1 cut(s)	158
Bme1390I	CCNGG	6 cut(s)	516, 782, 1007, 1082, 1316, 1469
Bme18I	GGWCC	4 cut(s)	505, 1078, 1285, 1410
BmeT110I	CYCGRG	5 cut(s)	350, 406, 461, 1142, 1266
BmgBI	CACGTC	2 cut(s)	357, 1390
BmgT120I	GGNCC	6 cut(s)	505, 999, 1078, 1264, 1285, 1410
BmiI	GGNNCC	8 cut(s)	423, 506, 657, 779, 892, 1079, 1287, 1369
BmrFI	CCNGG	6 cut(s)	516, 782, 1007, 1082, 1316, 1469
BmsI	GCATC	2 cut(s)	1335, 1500
BoxI	GACNNNNGTC	1 cut(s)	932
BpuMI	CCSGG	5 cut(s)	782, 1007, 1082, 1316, 1469
BsaHI	GRCGYC	1 cut(s)	197
BsaJI	CCNNGG	5 cut(s)	407, 515, 1143, 1252, 1267
BsaWI	WCCGGW	2 cut(s)	8, 929
BsaXI	ACNNNNNCTCC	2 cut(s)	1010, 1040
Bse118I	RCCGGY	5 cut(s)	8, 40, 161, 242, 275
Bse1I	ACTGG	1 cut(s)	198
BseBI	CCWGG	1 cut(s)	516
BseDI	CCNNGG	5 cut(s)	407, 515, 1143, 1252, 1267
BseGI	GGATG	2 cut(s)	1002, 1287
BseMII	CTCAG	2 cut(s)	459, 523
BseNI	ACTGG	1 cut(s)	198
BseRI	GAGGAG	1 cut(s)	953
BseSI	GKGCMC	1 cut(s)	1442
BseX3I	CGGCCG	1 cut(s)	1008
BseXI	GCAGC	7 cut(s)	41, 374, 827, 1405, 1507, 1529, 1535
Bsh1236I	CGCG	5 cut(s)	72, 310, 548, 1094, 1453
Bsh1285I	CGRYCG	5 cut(s)	52, 70, 607, 786, 1011
BshFI	GGCC	7 cut(s)	5, 161, 542, 924, 1000, 1010, 1265
BshTI	ACCGGT	1 cut(s)	8
BsiEI	CGRYCG	5 cut(s)	52, 70, 607, 786, 1011
BsiHKAI	GWGCWC	3 cut(s)	128, 1215, 1442
BsiHKCI	CYCGRG	5 cut(s)	350, 406, 461, 1142, 1266
BsiWI	CGTACG	4 cut(s)	871, 1320, 1350, 1514
BslFI	GGGAC	4 cut(s)	478, 1091, 1202, 1270
BsmAI	GTCTC	5 cut(s)	204, 235, 292, 347, 1037
BsmBI	CGTCTC	3 cut(s)	204, 347, 1037
BsmFI	GGGAC	4 cut(s)	478, 1091, 1202, 1270
BsmI	GAATGC	1 cut(s)	246
BsnI	GGCC	7 cut(s)	5, 161, 542, 924, 1000, 1010, 1265
BsoBI	CYCGRG	5 cut(s)	350, 406, 461, 1142, 1266
Bsp1286I	GDGCHC	3 cut(s)	128, 1215, 1442
Bsp1407I	TGTACA	1 cut(s)	879
Bsp143I	GATC	4 cut(s)	315, 471, 777, 1126
Bsp68I	TCGCGA	1 cut(s)	72
BspANI	GGCC	7 cut(s)	5, 161, 542, 924, 1000, 1010, 1265
BspCNI	CTCAG	2 cut(s)	460, 522
BspFNI	CGCG	5 cut(s)	72, 310, 548, 1094, 1453
BspHI	TCATGA	2 cut(s)	627, 1302
BspLI	GGNNCC	8 cut(s)	423, 506, 657, 779, 892, 1079, 1287, 1369
BspMAI	CTGCAG	1 cut(s)	1525
BspPI	GGATC	3 cut(s)	310, 772, 785
BsrBI	CCGCTC	1 cut(s)	751
BsrFI	RCCGGY	5 cut(s)	8, 40, 161, 242, 275
BsrGI	TGTACA	1 cut(s)	879
BsrI	ACTGG	1 cut(s)	198
BssAI	RCCGGY	5 cut(s)	8, 40, 161, 242, 275
BssECI	CCNNGG	5 cut(s)	407, 515, 1143, 1252, 1267
BssMI	GATC	4 cut(s)	315, 471, 777, 1126
BssNAI	GTATAC	1 cut(s)	847
BssNI	GRCGYC	1 cut(s)	197
Bst1107I	GTATAC	1 cut(s)	847
Bst2UI	CCWGG	1 cut(s)	516
Bst4CI	ACNGT	2 cut(s)	85, 1063
Bst6I	CTCTTC	1 cut(s)	33
BstACI	GRCGYC	1 cut(s)	197
BstAUI	TGTACA	1 cut(s)	879
BstDEI	CTNAG	6 cut(s)	113, 173, 468, 509, 1114, 1509
BstDSI	CCRYGG	1 cut(s)	1252
BstF5I	GGATG	2 cut(s)	1002, 1287
BstFNI	CGCG	5 cut(s)	72, 310, 548, 1094, 1453
BstH2I	RGCGCY	1 cut(s)	450
BstHHI	GCGC	4 cut(s)	310, 449, 550, 824
BstKTI	GATC	4 cut(s)	318, 474, 780, 1129
BstMAI	GTCTC	5 cut(s)	204, 235, 292, 347, 1037
BstMBI	GATC	4 cut(s)	315, 471, 777, 1126
BstMCI	CGRYCG	5 cut(s)	52, 70, 607, 786, 1011
BstNI	CCWGG	1 cut(s)	516
BstNSI	RCATGY	2 cut(s)	1249, 1405
BstPAI	GACNNNNGTC	1 cut(s)	932
BstSCI	CCNGG	6 cut(s)	514, 780, 1005, 1080, 1314, 1467
BstSFI	CTRYAG	2 cut(s)	1221, 1521
BstSLI	GKGCMC	1 cut(s)	1442
BstUI	CGCG	5 cut(s)	72, 310, 548, 1094, 1453
BstV1I	GCAGC	7 cut(s)	41, 374, 827, 1405, 1507, 1529, 1535
BstX2I	RGATCY	1 cut(s)	777
BstYI	RGATCY	1 cut(s)	777
BstZ17I	GTATAC	1 cut(s)	847
BstZI	CGGCCG	1 cut(s)	1008
BsuRI	GGCC	7 cut(s)	5, 161, 542, 924, 1000, 1010, 1265
BtgI	CCRYGG	1 cut(s)	1252
BtgZI	GCGATG	2 cut(s)	729, 1399
BtrI	CACGTC	2 cut(s)	357, 1390
BtsCI	GGATG	2 cut(s)	1002, 1287
BtsI	GCAGTG	1 cut(s)	824
BtsIMutI	CAGTG	3 cut(s)	81, 567, 824
BtuMI	TCGCGA	1 cut(s)	72
CaiI	CAGNNNCTG	1 cut(s)	587
CciI	TCATGA	2 cut(s)	627, 1302
CfoI	GCGC	4 cut(s)	310, 449, 550, 824
Cfr10I	RCCGGY	5 cut(s)	8, 40, 161, 242, 275
Cfr13I	GGNCC	6 cut(s)	505, 999, 1078, 1264, 1285, 1410
CseI	GACGC	2 cut(s)	186, 1100
Csp6I	GTAC	7 cut(s)	220, 872, 880, 1321, 1351, 1465, 1515
CspAI	ACCGGT	1 cut(s)	8
CviAII	CATG	9 cut(s)	97, 256, 454, 628, 1228, 1240, 1246, 1303, 1402
CviQI	GTAC	7 cut(s)	220, 872, 880, 1321, 1351, 1465, 1515
DdeI	CTNAG	6 cut(s)	113, 173, 468, 509, 1114, 1509
DpnI	GATC	4 cut(s)	317, 473, 779, 1128
DpnII	GATC	4 cut(s)	315, 471, 777, 1126
DraI	TTTAAA	1 cut(s)	1338
DrdI	GACNNNNNNGTC	1 cut(s)	1493
DseDI	GACNNNNNNGTC	1 cut(s)	1493
EaeI	YGGCCR	2 cut(s)	3, 1008
EagI	CGGCCG	1 cut(s)	1008
Eam1104I	CTCTTC	1 cut(s)	33
EarI	CTCTTC	1 cut(s)	33
EciI	GGCGGA	3 cut(s)	107, 325, 1174
Ecl136II	GAGCTC	2 cut(s)	126, 1213
EclXI	CGGCCG	1 cut(s)	1008
Eco24I	GRGCYC	2 cut(s)	128, 1215
Eco47I	GGWCC	4 cut(s)	505, 1078, 1285, 1410
Eco52I	CGGCCG	1 cut(s)	1008
Eco53kI	GAGCTC	2 cut(s)	126, 1213
Eco88I	CYCGRG	5 cut(s)	350, 406, 461, 1142, 1266
EcoICRI	GAGCTC	2 cut(s)	126, 1213
EcoO109I	RGGNCCY	1 cut(s)	505
EcoRII	CCWGG	1 cut(s)	514
EcoT38I	GRGCYC	2 cut(s)	128, 1215
Esp3I	CGTCTC	3 cut(s)	204, 347, 1037
FaeI	CATG	9 cut(s)	100, 259, 457, 631, 1231, 1243, 1249, 1306, 1405
FalI	AAGNNNNNCTT	2 cut(s)	12, 44
FaqI	GGGAC	4 cut(s)	478, 1091, 1202, 1270
FatI	CATG	9 cut(s)	96, 255, 453, 627, 1227, 1239, 1245, 1302, 1401
FauI	CCCGC	3 cut(s)	811, 913, 1471
FauNDI	CATATG	1 cut(s)	1136
FblI	GTMKAC	2 cut(s)	528, 846
FokI	GGATG	2 cut(s)	989, 1294
FriOI	GRGCYC	2 cut(s)	128, 1215
FspBI	CTAG	2 cut(s)	1311, 1546
GlaI	GCGC	4 cut(s)	309, 448, 549, 823
HaeII	RGCGCY	1 cut(s)	450
HaeIII	GGCC	7 cut(s)	5, 161, 542, 924, 1000, 1010, 1265
HgaI	GACGC	2 cut(s)	186, 1100
HhaI	GCGC	4 cut(s)	310, 449, 550, 824
Hin1I	GRCGYC	1 cut(s)	197
Hin1II	CATG	9 cut(s)	100, 259, 457, 631, 1231, 1243, 1249, 1306, 1405
Hin6I	GCGC	4 cut(s)	308, 447, 548, 822
HinP1I	GCGC	4 cut(s)	308, 447, 548, 822
HincII	GTYRAC	1 cut(s)	529
HindII	GTYRAC	1 cut(s)	529
HinfI	GANTC	7 cut(s)	236, 285, 299, 363, 933, 1068, 1179
HphI	GGTGA	1 cut(s)	694
Hpy166II	GTNNAC	4 cut(s)	529, 847, 880, 1440
Hpy188I	TCNGA	6 cut(s)	235, 292, 396, 738, 1039, 1067
Hpy188III	TCNNGA	6 cut(s)	71, 463, 587, 628, 719, 1303
Hpy8I	GTNNAC	4 cut(s)	529, 847, 880, 1440
Hpy99I	CGWCG	7 cut(s)	152, 533, 725, 845, 1264, 1391, 1499
HpyAV	CCTTC	9 cut(s)	10, 14, 69, 139, 427, 720, 1270, 1450, 1456
HpyCH4III	ACNGT	2 cut(s)	85, 1063
HpyCH4IV	ACGT	4 cut(s)	356, 1154, 1331, 1389
HpyCH4V	TGCA	7 cut(s)	346, 639, 1196, 1401, 1405, 1440, 1523
HpyF3I	CTNAG	6 cut(s)	113, 173, 468, 509, 1114, 1509
HpySE526I	ACGT	4 cut(s)	356, 1154, 1331, 1389
Hsp92I	GRCGYC	1 cut(s)	197
Hsp92II	CATG	9 cut(s)	100, 259, 457, 631, 1231, 1243, 1249, 1306, 1405
HspAI	GCGC	4 cut(s)	308, 447, 548, 822
KroI	GCCGGC	3 cut(s)	40, 161, 242
KroNI	GCCGGC	3 cut(s)	42, 163, 244
Kzo9I	GATC	4 cut(s)	315, 471, 777, 1126
LmnI	GCTCC	5 cut(s)	123, 131, 421, 589, 1367
Lsp1109I	GCAGC	7 cut(s)	41, 374, 827, 1405, 1507, 1529, 1535
LweI	GCATC	2 cut(s)	1335, 1500
MaeI	CTAG	2 cut(s)	1311, 1546
MaeII	ACGT	4 cut(s)	356, 1154, 1331, 1389
MaeIII	GTNAC	2 cut(s)	1012, 1327
MalI	GATC	4 cut(s)	317, 473, 779, 1128
MbiI	CCGCTC	1 cut(s)	751
MboI	GATC	4 cut(s)	315, 471, 777, 1126
MboII	GAAGA	3 cut(s)	20, 363, 1291
MflI	RGATCY	1 cut(s)	777
MhlI	GDGCHC	3 cut(s)	128, 1215, 1442
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	4 cut(s)	768, 1053, 1396, 1533
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	245, 294, 372, 942, 1062
MmeI	TCCRAC	2 cut(s)	315, 1090
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	3 cut(s)	40, 161, 242
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	4 cut(s)	552, 1056, 1104, 1337
MslI	CAYNNNNRTG	3 cut(s)	11, 86, 1244
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	54, 818
MspR9I	CCNGG	6 cut(s)	516, 782, 1007, 1082, 1316, 1469
Mva1269I	GAATGC	1 cut(s)	246
MvaI	CCWGG	1 cut(s)	516
MvnI	CGCG	5 cut(s)	72, 310, 548, 1094, 1453
NaeI	GCCGGC	3 cut(s)	42, 163, 244
NciI	CCSGG	5 cut(s)	782, 1007, 1082, 1316, 1469
NdeI	CATATG	1 cut(s)	1136
NdeII	GATC	4 cut(s)	315, 471, 777, 1126
NgoMIV	GCCGGC	3 cut(s)	40, 161, 242
NlaIII	CATG	9 cut(s)	100, 259, 457, 631, 1231, 1243, 1249, 1306, 1405
NlaIV	GGNNCC	8 cut(s)	423, 506, 657, 779, 892, 1079, 1287, 1369
NmeAIII	GCCGAG	1 cut(s)	1184
NmuCI	GTSAC	1 cut(s)	1012
NruI	TCGCGA	1 cut(s)	72
NspI	RCATGY	2 cut(s)	1249, 1405
OliI	CACNNNNGTG	1 cut(s)	86
PaeI	GCATGC	1 cut(s)	1405
PagI	TCATGA	2 cut(s)	627, 1302
PcsI	WCGNNNNNNNCGW	2 cut(s)	600, 1494
PctI	GAATGC	1 cut(s)	246
PdiI	GCCGGC	3 cut(s)	42, 163, 244
PfeI	GAWTC	2 cut(s)	299, 1179
Pfl23II	CGTACG	4 cut(s)	871, 1320, 1350, 1514
PflFI	GACNNNGTC	1 cut(s)	1082
PflMI	CCANNNNNTGG	2 cut(s)	209, 501
PinAI	ACCGGT	1 cut(s)	8
PleI	GAGTC	5 cut(s)	244, 293, 371, 941, 1062
PpsI	GAGTC	5 cut(s)	244, 293, 371, 941, 1062
PpuMI	RGGWCCY	1 cut(s)	505
PshAI	GACNNNNGTC	1 cut(s)	932
PsiI	TTATAA	1 cut(s)	767
Psp124BI	GAGCTC	2 cut(s)	128, 1215
Psp1406I	AACGTT	1 cut(s)	1331
Psp5II	RGGWCCY	1 cut(s)	505
Psp6I	CCWGG	1 cut(s)	514
PspGI	CCWGG	1 cut(s)	514
PspLI	CGTACG	4 cut(s)	871, 1320, 1350, 1514
PspN4I	GGNNCC	8 cut(s)	423, 506, 657, 779, 892, 1079, 1287, 1369
PspPI	GGNCC	6 cut(s)	505, 999, 1078, 1264, 1285, 1410
PspPPI	RGGWCCY	1 cut(s)	505
PstI	CTGCAG	1 cut(s)	1525
PstNI	CAGNNNCTG	1 cut(s)	587
PsuI	RGATCY	1 cut(s)	777
PsyI	GACNNNGTC	1 cut(s)	1082
RruI	TCGCGA	1 cut(s)	72
RsaI	GTAC	7 cut(s)	221, 873, 881, 1322, 1352, 1466, 1516
RsaNI	GTAC	7 cut(s)	220, 872, 880, 1321, 1351, 1465, 1515
RseI	CAYNNNNRTG	3 cut(s)	11, 86, 1244
SacI	GAGCTC	2 cut(s)	128, 1215
SalI	GTCGAC	1 cut(s)	527
SaqAI	TTAA	4 cut(s)	552, 1056, 1104, 1337
Sau3AI	GATC	4 cut(s)	315, 471, 777, 1126
Sau96I	GGNCC	6 cut(s)	505, 999, 1078, 1264, 1285, 1410
SchI	GAGTC	5 cut(s)	245, 294, 372, 942, 1062
ScrFI	CCNGG	6 cut(s)	516, 782, 1007, 1082, 1316, 1469
SduI	GDGCHC	3 cut(s)	128, 1215, 1442
SfaNI	GCATC	2 cut(s)	1335, 1500
SfcI	CTRYAG	2 cut(s)	1221, 1521
SinI	GGWCC	4 cut(s)	505, 1078, 1285, 1410
SmiMI	CAYNNNNRTG	3 cut(s)	11, 86, 1244
SphI	GCATGC	1 cut(s)	1405
Sse9I	AATT	4 cut(s)	768, 1053, 1396, 1533
SspMI	CTAG	2 cut(s)	1311, 1546
SstI	GAGCTC	2 cut(s)	128, 1215
StyD4I	CCNGG	6 cut(s)	514, 780, 1005, 1080, 1314, 1467
TaaI	ACNGT	2 cut(s)	85, 1063
TaiI	ACGT	4 cut(s)	359, 1157, 1334, 1392
TaqI	TCGA	6 cut(s)	48, 528, 690, 966, 1379, 1497
TaqII	GACCGA	2 cut(s)	56, 621
TasI	AATT	4 cut(s)	768, 1053, 1396, 1533
TatI	WGTACW	2 cut(s)	219, 879
TauI	GCSGC	3 cut(s)	168, 680, 751
TfiI	GAWTC	2 cut(s)	299, 1179
Tru1I	TTAA	4 cut(s)	552, 1056, 1104, 1337
Tru9I	TTAA	4 cut(s)	552, 1056, 1104, 1337
TscAI	CASTG	3 cut(s)	88, 574, 831
TseFI	GTSAC	1 cut(s)	1012
TseI	GCWGC	7 cut(s)	54, 387, 815, 1418, 1520, 1523, 1542
Tsp45I	GTSAC	1 cut(s)	1012
TspDTI	ATGAA	6 cut(s)	29, 616, 644, 931, 1291, 1319
TspGWI	ACGGA	4 cut(s)	136, 546, 629, 738
TspRI	CASTG	3 cut(s)	88, 574, 831
Tth111I	GACNNNGTC	1 cut(s)	1082
Van91I	CCANNNNNTGG	2 cut(s)	209, 501
VneI	GTGCAC	1 cut(s)	1438
VpaK11BI	GGWCC	4 cut(s)	505, 1078, 1285, 1410
XceI	RCATGY	2 cut(s)	1249, 1405
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1534
XmiI	GTMKAC	2 cut(s)	528, 846
XspI	CTAG	2 cut(s)	1311, 1546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.