Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Reverse (-)

19172972 .. 19182377

Gene Structure 9406 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG127000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGCTAGAACCACCTTTCCAACCATCGACAAATGCTCATCGAACGGGCGAGAAAACCACACGGTGGTTGCGGACATGGACGGGACCTTGCTAAGAGGTCGCATCTCTTTTCCTTACTTTGCTCTCATTGCTTTTGAGGTCGGGGGGATTTTAAGGCTCCTATTCTTGCTCTTGGTCTCCCCATTAGCTGGACTCCTCTATTACTTCGTTTCTGAATCGGCTGGGATCCAAGTTCAGATCTTCGCAACATTTGCCGGTGTGAGGGTGTCTGAGATCGAGCTGGTGGCGCGTGCCGTGCTGCCTAGGTTTTACTCGAGTGACATTCACCCGAAGTCGTGGCGCGTGCTTTCTGCCTGCGAGAAGCGGTGTATTCTTACTGCCAACCCTAGGATTATGGTGGAAGCGTTTTGTAAGGATTTCCTTGGATTTGATACGGTTTTGGGAACTGAAATTGCAACCTACAAAGGTAAAGCCACTGGCTTTGTTTCTCCACCTGGTGTGCTTGTAGCAGAGAAGAAGGCTGATGCTCTTAAGAAGGCTTTTGGAGATGTACAGCCAGAGATTGGGCTTGGCGATAGGCACACTGATATTCCTTTCATGGCACTTTGCAAGGAGGGTTATATGGTTCCGCCCAACCCTAAAGTAGAAGCAGTGACCGGAGATAAGCTCCCTAAGCCCATAGTCTTCCACGACGGCCGCCTAGTCCAAAAGCCAACACCTCTAATGGCCCTCCTCACCATCCTCTGGCTCCCCATCGGCTTCTTCCTTGCCTGTCTTCGAATCGCCGTTGGCTTCCTCCTCCCCATGCCAGTCGTCTACTACGCCTTCTGGGCCCTCGGTGTTCGTGTCACCGTTAAGGGCACCCCACCCCCTCCAGCCAAAAAATCCACAGGCCAGTCTGGCGTCCTCTTCATCTGCTCCCACCGCACCCTCCTCGACCCCGTTTTCCTCTCCATCGCCCTCGGTCGCCCCATCCCCACCGTCACCTACTCCGTCTCTCGCCTCACAGAGTTCCTCTCCCCCATCAAGACCGTCCGCCTCAACCGCGACCGCGCCAAAGACGCTGCCATGATCAAGAAGCTACTAGAAGAAGGTGACCTTGCAATCTGCCCCGAGGGGACCACCTGCAGGGAGCCATTTCTGCTGAGGTTCTCAGCGCTTTTTGCTGAGCTAACCGATGAGCTCGTTCCGGTGGCGATGGTGAACAAGATGAGCATGTTCCACTGCACGACGGCGCTTGGGTGGAAGGGGAAGGGCCCTTTTTACTTCTTCATGAACCCTCGCCCAGAATACGAGGTTACATTTTTAAACAAGCTGCCGGCGGAGTTGACTTGCGGGGCGGGCAAGTCGAGCCATGAGGTGGCGAATTATATACAGAAGGCTATAGCGGCCAGCCTTTCGTATGATATCACCAGTTTTACGAGGAAAGACAAGTATAGGGCTTTGGCTGGGAACGATGGCTCTGTGGTTGAGAAACCTGTGCTGCAACCCAACAAAGTCATGGGGTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

503

Amino Acids

55.29

Weight (kDa)

9.16

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	21 - 209	1.3e-58	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	22 - 200	5.4e-23	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	299 - 399	2.8e-07	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1133
Acc36I	ACCTGC	1 cut(s)	1133
AccB1I	GGYRCC	1 cut(s)	860
AccB7I	CCANNNNNTGG	5 cut(s)	64, 188, 563, 744, 1360
AccI	GTMKAC	1 cut(s)	816
AccII	CGCG	4 cut(s)	289, 342, 1047, 1053
AclWI	GGATC	2 cut(s)	220, 233
AcoI	YGGCCR	2 cut(s)	694, 1389
AcyI	GRCGYC	1 cut(s)	903
AdeI	CACNNNGTG	2 cut(s)	64, 497
AfaI	GTAC	1 cut(s)	552
AfeI	AGCGCT	1 cut(s)	1158
AfiI	CCNNNNNNNGG	8 cut(s)	64, 188, 261, 387, 563, 744, 1128, 1360
AflII	CTTAAG	1 cut(s)	530
AjnI	CCWGG	1 cut(s)	493
AloI	GAACNNNNNNTCC	1 cut(s)	33
AluBI	AGCT	7 cut(s)	188, 280, 667, 1081, 1171, 1183, 1315
AluI	AGCT	7 cut(s)	188, 280, 667, 1081, 1171, 1183, 1315
Alw21I	GWGCWC	1 cut(s)	1185
Alw26I	GTCTC	2 cut(s)	181, 1000
AlwI	GGATC	2 cut(s)	220, 233
Ama87I	CYCGRG	2 cut(s)	313, 1112
Aor51HI	AGCGCT	1 cut(s)	1158
AoxI	GGCC	6 cut(s)	694, 726, 831, 892, 1255, 1389
ApaI	GGGCCC	2 cut(s)	835, 1259
ApeKI	GCWGC	4 cut(s)	298, 1064, 1315, 1483
AspA2I	CCTAGG	2 cut(s)	302, 386
AspLEI	GCGC	5 cut(s)	289, 342, 1055, 1159, 1237
AspS9I	GGNCC	7 cut(s)	84, 727, 831, 832, 1119, 1255, 1256
AsuHPI	GGTGA	7 cut(s)	317, 727, 841, 976, 1106, 1213, 1402
AsuII	TTCGAA	1 cut(s)	778
AvaI	CYCGRG	2 cut(s)	313, 1112
AvaII	GGWCC	2 cut(s)	84, 1119
AvrII	CCTAGG	2 cut(s)	302, 386
BaeGI	GKGCMC	3 cut(s)	835, 863, 1259
BamHI	GGATCC	1 cut(s)	225
BanI	GGYRCC	1 cut(s)	860
BanII	GRGCYC	3 cut(s)	835, 1185, 1259
BbsI	GAAGAC	2 cut(s)	676, 767
Bbv12I	GWGCWC	1 cut(s)	1185
BbvCI	CCTCAGC	1 cut(s)	1145
BbvI	GCAGC	4 cut(s)	285, 1051, 1302, 1470
BccI	CCATC	8 cut(s)	32, 746, 761, 962, 980, 1031, 1192, 1451
BceAI	ACGGC	4 cut(s)	278, 709, 770, 1248
BcgI	CGANNNNNNTGC	2 cut(s)	916, 950
BciT130I	CCWGG	1 cut(s)	495
BclI	TGATCA	1 cut(s)	1071
BcoDI	GTCTC	2 cut(s)	181, 1000
BfaI	CTAG	5 cut(s)	6, 303, 387, 701, 1085
BfmI	CTRYAG	2 cut(s)	1126, 1383
BfoI	RGCGCY	2 cut(s)	1160, 1238
BfrI	CTTAAG	1 cut(s)	530
BfuAI	ACCTGC	1 cut(s)	1133
BglI	GCCNNNNNGGC	2 cut(s)	830, 900
BglII	AGATCT	1 cut(s)	237
BisI	GCNGC	6 cut(s)	299, 697, 1065, 1316, 1389, 1484
BlnI	CCTAGG	2 cut(s)	302, 386
BlpI	GCTNAGC	1 cut(s)	1167
BlsI	GCNGC	6 cut(s)	300, 698, 1066, 1317, 1390, 1485
Bme1390I	CCNGG	1 cut(s)	495
Bme18I	GGWCC	2 cut(s)	84, 1119
BmeT110I	CYCGRG	2 cut(s)	313, 1112
BmgT120I	GGNCC	7 cut(s)	84, 727, 831, 832, 1119, 1255, 1256
BmrFI	CCNGG	1 cut(s)	495
BmsI	GCATC	2 cut(s)	111, 514
BpiI	GAAGAC	2 cut(s)	676, 767
BplI	GAGNNNNNCTC	4 cut(s)	651, 683, 1001, 1033
BpmI	CTGGAG	1 cut(s)	858
Bpu10I	CCTNAGC	2 cut(s)	672, 1145
Bpu1102I	GCTNAGC	1 cut(s)	1167
Bpu14I	TTCGAA	1 cut(s)	778
BsaHI	GRCGYC	1 cut(s)	903
BsaI	GGTCTC	1 cut(s)	181
BsaJI	CCNNGG	6 cut(s)	302, 386, 421, 835, 961, 1113
BsaWI	WCCGGW	2 cut(s)	656, 1189
Bsc4I	CCNNNNNNNGG	8 cut(s)	64, 188, 261, 387, 563, 744, 1128, 1360
Bse118I	RCCGGY	2 cut(s)	254, 1318
Bse1I	ACTGG	4 cut(s)	481, 809, 895, 1413
Bse3DI	GCAATG	1 cut(s)	126
BseBI	CCWGG	1 cut(s)	495
BseDI	CCNNGG	6 cut(s)	302, 386, 421, 835, 961, 1113
BseGI	GGATG	2 cut(s)	738, 972
BseLI	CCNNNNNNNGG	8 cut(s)	64, 188, 261, 387, 563, 744, 1128, 1360
BseMI	GCAATG	1 cut(s)	126
BseMII	CTCAG	4 cut(s)	261, 1136, 1158, 1167
BseNI	ACTGG	4 cut(s)	481, 809, 895, 1413
BseRI	GAGGAG	4 cut(s)	185, 722, 788, 923
BseSI	GKGCMC	3 cut(s)	835, 863, 1259
BseX3I	CGGCCG	1 cut(s)	694
BseXI	GCAGC	4 cut(s)	285, 1051, 1302, 1470
BseYI	CCCAGC	2 cut(s)	221, 1448
BsgI	GTGCAG	1 cut(s)	1210
Bsh1236I	CGCG	4 cut(s)	289, 342, 1047, 1053
Bsh1285I	CGRYCG	3 cut(s)	697, 967, 1051
BshFI	GGCC	6 cut(s)	696, 728, 833, 894, 1257, 1391
BshNI	GGYRCC	1 cut(s)	860
BsiEI	CGRYCG	3 cut(s)	697, 967, 1051
BsiHKAI	GWGCWC	1 cut(s)	1185
BsiHKCI	CYCGRG	2 cut(s)	313, 1112
BsiSI	CCGG	4 cut(s)	255, 657, 1190, 1319
BslFI	GGGAC	2 cut(s)	97, 1132
BslI	CCNNNNNNNGG	8 cut(s)	64, 188, 261, 387, 563, 744, 1128, 1360
BsmAI	GTCTC	2 cut(s)	181, 1000
BsmBI	CGTCTC	1 cut(s)	1000
BsmFI	GGGAC	2 cut(s)	97, 1132
BsnI	GGCC	6 cut(s)	696, 728, 833, 894, 1257, 1391
Bso31I	GGTCTC	1 cut(s)	181
BsoBI	CYCGRG	2 cut(s)	313, 1112
Bsp119I	TTCGAA	1 cut(s)	778
Bsp120I	GGGCCC	2 cut(s)	831, 1255
Bsp1286I	GDGCHC	4 cut(s)	835, 863, 1185, 1259
Bsp1407I	TGTACA	1 cut(s)	550
Bsp143I	GATC	4 cut(s)	225, 237, 273, 1071
Bsp1720I	GCTNAGC	1 cut(s)	1167
BspANI	GGCC	6 cut(s)	696, 728, 833, 894, 1257, 1391
BspCNI	CTCAG	4 cut(s)	262, 1137, 1159, 1166
BspFNI	CGCG	4 cut(s)	289, 342, 1047, 1053
BspHI	TCATGA	1 cut(s)	1272
BspMAI	CTGCAG	1 cut(s)	1130
BspMI	ACCTGC	1 cut(s)	1133
BspPI	GGATC	2 cut(s)	220, 233
BspT104I	TTCGAA	1 cut(s)	778
BspT107I	GGYRCC	1 cut(s)	860
BspTI	CTTAAG	1 cut(s)	530
BspTNI	GGTCTC	1 cut(s)	181
BsrDI	GCAATG	1 cut(s)	126
BsrFI	RCCGGY	2 cut(s)	254, 1318
BsrGI	TGTACA	1 cut(s)	550
BsrI	ACTGG	4 cut(s)	481, 809, 895, 1413
BssAI	RCCGGY	2 cut(s)	254, 1318
BssECI	CCNNGG	6 cut(s)	302, 386, 421, 835, 961, 1113
BssMI	GATC	4 cut(s)	225, 237, 273, 1071
BssNI	GRCGYC	1 cut(s)	903
BssT1I	CCWWGG	3 cut(s)	302, 386, 421
Bst2UI	CCWGG	1 cut(s)	495
Bst4CI	ACNGT	5 cut(s)	64, 436, 853, 982, 1033
Bst6I	CTCTTC	1 cut(s)	914
BstACI	GRCGYC	1 cut(s)	903
BstAFI	CTTAAG	1 cut(s)	530
BstAPI	GCANNNNNTGC	1 cut(s)	251
BstAUI	TGTACA	1 cut(s)	550
BstBI	TTCGAA	1 cut(s)	778
BstC8I	GCNNGC	6 cut(s)	291, 344, 355, 1320, 1342, 1393
BstDEI	CTNAG	6 cut(s)	92, 270, 672, 1145, 1153, 1167
BstEII	GGTNACC	1 cut(s)	1094
BstF5I	GGATG	2 cut(s)	738, 972
BstFNI	CGCG	4 cut(s)	289, 342, 1047, 1053
BstH2I	RGCGCY	2 cut(s)	1160, 1238
BstHHI	GCGC	5 cut(s)	289, 342, 1055, 1159, 1237
BstKTI	GATC	4 cut(s)	228, 240, 276, 1074
BstMAI	GTCTC	2 cut(s)	181, 1000
BstMBI	GATC	4 cut(s)	225, 237, 273, 1071
BstMCI	CGRYCG	3 cut(s)	697, 967, 1051
BstNI	CCWGG	1 cut(s)	495
BstNSI	RCATGY	1 cut(s)	1219
BstPI	GGTNACC	1 cut(s)	1094
BstSCI	CCNGG	1 cut(s)	493
BstSFI	CTRYAG	2 cut(s)	1126, 1383
BstSLI	GKGCMC	3 cut(s)	835, 863, 1259
BstUI	CGCG	4 cut(s)	289, 342, 1047, 1053
BstV1I	GCAGC	4 cut(s)	285, 1051, 1302, 1470
BstV2I	GAAGAC	2 cut(s)	676, 767
BstX2I	RGATCY	2 cut(s)	225, 237
BstYI	RGATCY	2 cut(s)	225, 237
BstZI	CGGCCG	1 cut(s)	694
BsuRI	GGCC	6 cut(s)	696, 728, 833, 894, 1257, 1391
BtgZI	GCGATG	2 cut(s)	940, 1211
BtsCI	GGATG	2 cut(s)	738, 972
BtsI	GCAGTG	2 cut(s)	657, 1222
BtsIMutI	CAGTG	4 cut(s)	474, 582, 657, 1222
BveI	ACCTGC	1 cut(s)	1133
Cac8I	GCNNGC	6 cut(s)	291, 344, 355, 1320, 1342, 1393
CciI	TCATGA	1 cut(s)	1272
CfoI	GCGC	5 cut(s)	289, 342, 1055, 1159, 1237
Cfr10I	RCCGGY	2 cut(s)	254, 1318
Cfr13I	GGNCC	7 cut(s)	84, 727, 831, 832, 1119, 1255, 1256
CseI	GACGC	2 cut(s)	892, 1070
CsiI	ACCWGGT	1 cut(s)	493
Csp6I	GTAC	1 cut(s)	551
CspCI	CAANNNNNGTGG	2 cut(s)	463, 498
CviAII	CATG	8 cut(s)	76, 598, 805, 1069, 1216, 1273, 1355, 1501
CviQI	GTAC	1 cut(s)	551
DdeI	CTNAG	6 cut(s)	92, 270, 672, 1145, 1153, 1167
DpnI	GATC	4 cut(s)	227, 239, 275, 1073
DpnII	GATC	4 cut(s)	225, 237, 273, 1071
DraI	TTTAAA	1 cut(s)	1308
DraIII	CACNNNGTG	2 cut(s)	64, 497
EaeI	YGGCCR	2 cut(s)	694, 1389
EagI	CGGCCG	1 cut(s)	694
Eam1104I	CTCTTC	1 cut(s)	914
EarI	CTCTTC	1 cut(s)	914
EciI	GGCGGA	3 cut(s)	618, 1025, 1337
Ecl136II	GAGCTC	1 cut(s)	1183
EclXI	CGGCCG	1 cut(s)	694
Eco130I	CCWWGG	3 cut(s)	302, 386, 421
Eco24I	GRGCYC	3 cut(s)	835, 1185, 1259
Eco31I	GGTCTC	1 cut(s)	181
Eco32I	GATATC	1 cut(s)	1408
Eco47I	GGWCC	2 cut(s)	84, 1119
Eco47III	AGCGCT	1 cut(s)	1158
Eco52I	CGGCCG	1 cut(s)	694
Eco53kI	GAGCTC	1 cut(s)	1183
Eco88I	CYCGRG	2 cut(s)	313, 1112
Eco91I	GGTNACC	1 cut(s)	1094
EcoICRI	GAGCTC	1 cut(s)	1183
EcoO109I	RGGNCCY	4 cut(s)	84, 832, 1255, 1256
EcoO65I	GGTNACC	1 cut(s)	1094
EcoRII	CCWGG	1 cut(s)	493
EcoRV	GATATC	1 cut(s)	1408
EcoT14I	CCWWGG	3 cut(s)	302, 386, 421
EcoT38I	GRGCYC	3 cut(s)	835, 1185, 1259
ErhI	CCWWGG	3 cut(s)	302, 386, 421
Esp3I	CGTCTC	1 cut(s)	1000
FaeI	CATG	8 cut(s)	79, 601, 808, 1072, 1219, 1276, 1358, 1504
FalI	AAGNNNNNCTT	2 cut(s)	1083, 1115
FaqI	GGGAC	2 cut(s)	97, 1132
FatI	CATG	8 cut(s)	75, 597, 804, 1068, 1215, 1272, 1354, 1500
FauI	CCCGC	2 cut(s)	1328, 1333
FbaI	TGATCA	1 cut(s)	1071
FblI	GTMKAC	1 cut(s)	816
Fnu4HI	GCNGC	6 cut(s)	299, 697, 1065, 1316, 1389, 1484
FokI	GGATG	2 cut(s)	725, 959
FriOI	GRGCYC	3 cut(s)	835, 1185, 1259
Fsp4HI	GCNGC	6 cut(s)	299, 697, 1065, 1316, 1389, 1484
FspBI	CTAG	5 cut(s)	6, 303, 387, 701, 1085
GlaI	GCGC	5 cut(s)	288, 341, 1054, 1158, 1236
GluI	GCNGC	6 cut(s)	299, 697, 1065, 1316, 1389, 1484
GsaI	CCCAGC	2 cut(s)	225, 1452
GsuI	CTGGAG	1 cut(s)	858
HaeII	RGCGCY	2 cut(s)	1160, 1238
HaeIII	GGCC	6 cut(s)	696, 728, 833, 894, 1257, 1391
HapII	CCGG	4 cut(s)	255, 657, 1190, 1319
HgaI	GACGC	2 cut(s)	892, 1070
HhaI	GCGC	5 cut(s)	289, 342, 1055, 1159, 1237
Hin1I	GRCGYC	1 cut(s)	903
Hin1II	CATG	8 cut(s)	79, 601, 808, 1072, 1219, 1276, 1358, 1504
Hin6I	GCGC	5 cut(s)	287, 340, 1053, 1157, 1235
HinP1I	GCGC	5 cut(s)	287, 340, 1053, 1157, 1235
HincII	GTYRAC	1 cut(s)	1329
HindII	GTYRAC	1 cut(s)	1329
HinfI	GANTC	3 cut(s)	192, 215, 780
HpaII	CCGG	4 cut(s)	255, 657, 1190, 1319
HphI	GGTGA	7 cut(s)	317, 727, 841, 976, 1106, 1213, 1402
Hpy166II	GTNNAC	3 cut(s)	817, 1204, 1329
Hpy188I	TCNGA	3 cut(s)	214, 237, 271
Hpy188III	TCNNGA	3 cut(s)	1027, 1075, 1273
Hpy8I	GTNNAC	3 cut(s)	817, 1204, 1329
Hpy99I	CGWCG	2 cut(s)	695, 1234
HpyAV	CCTTC	7 cut(s)	511, 529, 835, 1085, 1240, 1246, 1372
HpyCH4III	ACNGT	5 cut(s)	64, 436, 853, 982, 1033
HpyCH4V	TGCA	6 cut(s)	455, 609, 1103, 1128, 1227, 1486
HpyF3I	CTNAG	6 cut(s)	92, 270, 672, 1145, 1153, 1167
Hsp92I	GRCGYC	1 cut(s)	903
Hsp92II	CATG	8 cut(s)	79, 601, 808, 1072, 1219, 1276, 1358, 1504
HspAI	GCGC	5 cut(s)	287, 340, 1053, 1157, 1235
KroI	GCCGGC	1 cut(s)	1318
KroNI	GCCGGC	1 cut(s)	1320
Ksp22I	TGATCA	1 cut(s)	1071
Kzo9I	GATC	4 cut(s)	225, 237, 273, 1071
LmnI	GCTCC	5 cut(s)	162, 672, 753, 923, 1132
Lsp1109I	GCAGC	4 cut(s)	285, 1051, 1302, 1470
LweI	GCATC	2 cut(s)	111, 514
MabI	ACCWGGT	1 cut(s)	493
MaeI	CTAG	5 cut(s)	6, 303, 387, 701, 1085
MaeIII	GTNAC	6 cut(s)	317, 652, 847, 982, 1094, 1297
MalI	GATC	4 cut(s)	227, 239, 275, 1073
MboI	GATC	4 cut(s)	225, 237, 273, 1071
MboII	GAAGA	8 cut(s)	232, 526, 676, 754, 767, 901, 1100, 1261
MflI	RGATCY	2 cut(s)	225, 237
MhlI	GDGCHC	4 cut(s)	835, 863, 1185, 1259
MluCI	AATT	2 cut(s)	450, 1366
MlyI	GAGTC	1 cut(s)	186
MmeI	TCCRAC	1 cut(s)	44
MroNI	GCCGGC	1 cut(s)	1318
MseI	TTAA	4 cut(s)	152, 531, 855, 1307
MspCI	CTTAAG	1 cut(s)	530
MspI	CCGG	4 cut(s)	255, 657, 1190, 1319
MspR9I	CCNGG	1 cut(s)	495
MvaI	CCWGG	1 cut(s)	495
MvnI	CGCG	4 cut(s)	289, 342, 1047, 1053
NaeI	GCCGGC	1 cut(s)	1320
NdeII	GATC	4 cut(s)	225, 237, 273, 1071
NgoMIV	GCCGGC	1 cut(s)	1318
NlaIII	CATG	8 cut(s)	79, 601, 808, 1072, 1219, 1276, 1358, 1504
NmuCI	GTSAC	5 cut(s)	317, 652, 847, 982, 1094
NspI	RCATGY	1 cut(s)	1219
NspV	TTCGAA	1 cut(s)	778
PaeR7I	CTCGAG	1 cut(s)	313
PagI	TCATGA	1 cut(s)	1272
PaqCI	CACCTGC	1 cut(s)	1133
PdiI	GCCGGC	1 cut(s)	1320
PfeI	GAWTC	2 cut(s)	215, 780
PflMI	CCANNNNNTGG	5 cut(s)	64, 188, 563, 744, 1360
PkrI	GCNGC	6 cut(s)	300, 698, 1066, 1317, 1390, 1485
PleI	GAGTC	1 cut(s)	186
PpsI	GAGTC	1 cut(s)	186
PpuMI	RGGWCCY	1 cut(s)	84
Psp124BI	GAGCTC	1 cut(s)	1185
Psp5II	RGGWCCY	1 cut(s)	84
Psp6I	CCWGG	1 cut(s)	493
PspEI	GGTNACC	1 cut(s)	1094
PspFI	CCCAGC	2 cut(s)	221, 1448
PspGI	CCWGG	1 cut(s)	493
PspOMI	GGGCCC	2 cut(s)	831, 1255
PspPI	GGNCC	7 cut(s)	84, 727, 831, 832, 1119, 1255, 1256
PspPPI	RGGWCCY	1 cut(s)	84
PspXI	VCTCGAGB	1 cut(s)	313
PstI	CTGCAG	1 cut(s)	1130
PsuI	RGATCY	2 cut(s)	225, 237
RsaI	GTAC	1 cut(s)	552
RsaNI	GTAC	1 cut(s)	551
SacI	GAGCTC	1 cut(s)	1185
SaqAI	TTAA	4 cut(s)	152, 531, 855, 1307
SatI	GCNGC	6 cut(s)	299, 697, 1065, 1316, 1389, 1484
Sau3AI	GATC	4 cut(s)	225, 237, 273, 1071
Sau96I	GGNCC	7 cut(s)	84, 727, 831, 832, 1119, 1255, 1256
SbfI	CCTGCAGG	1 cut(s)	1130
SchI	GAGTC	1 cut(s)	186
ScrFI	CCNGG	1 cut(s)	495
SdaI	CCTGCAGG	1 cut(s)	1130
SduI	GDGCHC	4 cut(s)	835, 863, 1185, 1259
SexAI	ACCWGGT	1 cut(s)	493
SfaNI	GCATC	2 cut(s)	111, 514
SfcI	CTRYAG	2 cut(s)	1126, 1383
Sfr274I	CTCGAG	1 cut(s)	313
SfuI	TTCGAA	1 cut(s)	778
SinI	GGWCC	2 cut(s)	84, 1119
SlaI	CTCGAG	1 cut(s)	313
SmlI	CTYRAG	2 cut(s)	313, 530
SmoI	CTYRAG	2 cut(s)	313, 530
Sse8387I	CCTGCAGG	1 cut(s)	1130
Sse9I	AATT	2 cut(s)	450, 1366
SspMI	CTAG	5 cut(s)	6, 303, 387, 701, 1085
SstI	GAGCTC	1 cut(s)	1185
StyD4I	CCNGG	1 cut(s)	493
StyI	CCWWGG	3 cut(s)	302, 386, 421
TaaI	ACNGT	5 cut(s)	64, 436, 853, 982, 1033
TaqI	TCGA	7 cut(s)	27, 41, 276, 314, 778, 936, 1349
TaqII	GACCGA	1 cut(s)	953
TasI	AATT	2 cut(s)	450, 1366
TatI	WGTACW	1 cut(s)	550
TauI	GCSGC	2 cut(s)	699, 1391
TfiI	GAWTC	2 cut(s)	215, 780
Tru1I	TTAA	4 cut(s)	152, 531, 855, 1307
Tru9I	TTAA	4 cut(s)	152, 531, 855, 1307
TscAI	CASTG	4 cut(s)	481, 589, 657, 1229
TseFI	GTSAC	5 cut(s)	317, 652, 847, 982, 1094
TseI	GCWGC	4 cut(s)	298, 1064, 1315, 1483
Tsp45I	GTSAC	5 cut(s)	317, 652, 847, 982, 1094
TspDTI	ATGAA	4 cut(s)	586, 901, 1261, 1289
TspGWI	ACGGA	1 cut(s)	982
TspRI	CASTG	4 cut(s)	481, 589, 657, 1229
Van91I	CCANNNNNTGG	5 cut(s)	64, 188, 563, 744, 1360
Vha464I	CTTAAG	1 cut(s)	530
VpaK11BI	GGWCC	2 cut(s)	84, 1119
XceI	RCATGY	1 cut(s)	1219
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1498
XhoI	CTCGAG	1 cut(s)	313
XmaJI	CCTAGG	2 cut(s)	302, 386
XmiI	GTMKAC	1 cut(s)	816
XspI	CTAG	5 cut(s)	6, 303, 387, 701, 1085

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.