Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Forward (+)

78611740 .. 78613218

Gene Structure 1479 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G050890.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGCCCGTCCATCGGACGAGAAAAGACACTGTTGTCGCGGACATGGACGGGACTCTTCTCCGCGGACGGAGCTCATTTCCGTACTTCGCCCTCGTGGCTTTCGAAGTTGGCGGCATTTTGAGGCTTCTTTTTCTGCTCTTGGCCTCACCGCTTGCCGGCGTTCTCTACTACTTCGTCTCTGAGTCCGCCGGCATACGTGTACTCGTGTTCGGGACTTTCGCGGGCATGAAAGTCTCGGATATCGAGTCCGTGGCACGGGCCGTGCTGCCGAAATTCTACTCCACTGACTTACACTCCGAAACATGGAGAGTCTTCTCGTCGTGCGGGAAACGCTGTGTTTTGACGGCCAACCCGAGAATCATGGTGGAGGGGTTTTTGAAGGAGTTTTTGGGCGCTGACATGGTTCTCGGGACAGAGATTGCCACGTACAGAGGTAGAGCTACCGGCTTTGTCCTAAACCCTGGGATTCTTGTCGGAAACAACAAAGCCGACGCGCTTAAAAAAGAGTTTGGTTCCGACGCTCCGGATATCGGACTCGGTGACCGAAAAACTGACTTTCCGTTCATGAAGCTGTGCAAAGAGAGCTACGTTGTTCCGGCTAAGCCGGAAGTTGAGCCGGTGAGCCACGAAAAATTACCGAAGCCTATAGTGTTCCACGACGGCCGGCTCGCTTCGAAACTAACTCCGTTGATGGCTTTGCTCACCATTCTTTGGATTCCGGTCGGTTTCGTTTTGGCTTGCTTACGTATTGCTGCCGGTTCATTGCTTCCTATGCCGGTCGTGTACTATGCCTTTTGGGCTTTGGGTGTTAGGGTTTATATCAAGGGCACCCCACCACCTCCAGCTAAGAAGTCAACTGGTCAAACCGGAGTTCTCTTCATTTGCTCACACCGAACCCTACTCGATCCCATTTTCCTCTCCACCGCTCTCGGGCGTCCGATCCCGGCAGTCACATACTCTCTCTCGCGTCTCTCGGAGATAATCTCACCCATCAAAACCGTCCGGCTAACCCGGGACCGGGTTGCCGACGCTAACATGATCAAGAAGCTTCTAGAACAAGGTGACCTAGTCATATGCCCCGAGGGCACAACATGCCGAGAACCCTTTCTGCTGCGGTTCTCTGCGTTGTTTGCCGAGCTCACGGACGAGCTGGTCCCCGTGGCGATGGCAAATCGGATGAGCATGTTCCATGGAACCACCGCGAGGGGGTGGAAGGGGATGGACCCGTTTTACTTCTTCATGAACCCTAGCCCTGCTTATGAAGTAACGTTTTTGAACAAGTTGCCTAGCGAGTTAACTTGCGGAGCAGGCAAGTCGAGCCATGATGTGGCGAACTATATACAAAGGACTATCGCGGCGAGCTTATCGTACGAGTGCACAACGTTCACGAGGAAAGACAAGTACAGAGCTCTGGCCGGGAATGATGGCACGGTGGCGGAGAAACCAGCTGCTAATGCCAAAAAAATTATGGGGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

492

Amino Acids

53.94

Weight (kDa)

9.3

Isoelectric Point (pI)

35.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	10 - 198	1.1e-62	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	11 - 190	5.6e-24	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	288 - 387	2.1e-06	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	32, 1151
AccB1I	GGYRCC	1 cut(s)	827
AccB7I	CCANNNNNTGG	2 cut(s)	711, 1327
AccBSI	CCGCTC	1 cut(s)	926
AccII	CGCG	7 cut(s)	38, 63, 221, 494, 967, 1202, 1355
AccIII	TCCGGA	1 cut(s)	523
AclI	AACGTT	2 cut(s)	1268, 1382
AclWI	GGATC	2 cut(s)	899, 934
AcoI	YGGCCR	3 cut(s)	345, 661, 1413
AcsI	RAATTY	1 cut(s)	272
AcyI	GRCGYC	1 cut(s)	934
AfaI	GTAC	6 cut(s)	83, 201, 428, 785, 1370, 1403
AflIII	ACRYGT	1 cut(s)	196
AgsI	TTSAA	2 cut(s)	379, 1276
AjnI	CCWGG	1 cut(s)	460
AjuI	GAANNNNNNNTTGG	2 cut(s)	371, 403
Alw21I	GWGCWC	4 cut(s)	74, 1140, 1379, 1411
Alw26I	GTCTC	3 cut(s)	181, 238, 974
Alw44I	GTGCAC	1 cut(s)	1375
AlwI	GGATC	2 cut(s)	899, 934
Ama87I	CYCGRG	5 cut(s)	352, 407, 929, 1011, 1079
Aor13HI	TCCGGA	1 cut(s)	523
AoxI	GGCC	5 cut(s)	141, 258, 345, 661, 1413
ApaLI	GTGCAC	1 cut(s)	1375
ApeKI	GCWGC	4 cut(s)	265, 752, 1111, 1448
ApoI	RAATTY	1 cut(s)	272
Asp700I	GAANNNNTTC	1 cut(s)	1104
AspLEI	GCGC	2 cut(s)	395, 496
AspS9I	GGNCC	4 cut(s)	258, 1015, 1153, 1222
AsuC2I	CCSGG	5 cut(s)	944, 1012, 1013, 1019, 1417
AsuHPI	GGTGA	6 cut(s)	138, 551, 631, 694, 978, 1073
AsuII	TTCGAA	2 cut(s)	102, 674
AvaI	CYCGRG	5 cut(s)	352, 407, 929, 1011, 1079
AvaII	GGWCC	3 cut(s)	1015, 1153, 1222
BaeGI	GKGCMC	3 cut(s)	830, 1088, 1379
BanI	GGYRCC	1 cut(s)	827
BanII	GRGCYC	3 cut(s)	74, 1140, 1411
BauI	CACGAG	3 cut(s)	92, 203, 1387
BbsI	GAAGAC	1 cut(s)	304
Bbv12I	GWGCWC	4 cut(s)	74, 1140, 1379, 1411
BbvI	GCAGC	4 cut(s)	252, 739, 1098, 1435
BccI	CCATC	6 cut(s)	18, 685, 998, 1159, 1213, 1418
BceAI	ACGGC	3 cut(s)	245, 360, 676
BcgI	CGANNNNNNTGC	2 cut(s)	1296, 1330
BciT130I	CCWGG	1 cut(s)	462
BclI	TGATCA	1 cut(s)	1038
BcnI	CCSGG	5 cut(s)	944, 1012, 1013, 1019, 1417
BcoDI	GTCTC	3 cut(s)	181, 238, 974
BfaI	CTAG	5 cut(s)	1052, 1067, 1248, 1287, 1477
BfmI	CTRYAG	1 cut(s)	645
BfoI	RGCGCY	1 cut(s)	396
BglI	GCCNNNNNGGC	3 cut(s)	95, 797, 1083
BisI	GCNGC	6 cut(s)	112, 266, 753, 1112, 1356, 1449
BlpI	GCTNAGC	1 cut(s)	600
BlsI	GCNGC	6 cut(s)	113, 267, 754, 1113, 1357, 1450
Bme1390I	CCNGG	6 cut(s)	462, 944, 1012, 1013, 1019, 1417
Bme18I	GGWCC	3 cut(s)	1015, 1153, 1222
BmeT110I	CYCGRG	5 cut(s)	352, 407, 929, 1011, 1079
BmgT120I	GGNCC	4 cut(s)	258, 1015, 1153, 1222
BmiI	GGNNCC	6 cut(s)	514, 829, 1016, 1155, 1195, 1224
BmrFI	CCNGG	6 cut(s)	462, 944, 1012, 1013, 1019, 1417
BpiI	GAAGAC	1 cut(s)	304
BplI	GAGNNNNNCTC	2 cut(s)	968, 1000
BpmI	CTGGAG	1 cut(s)	825
Bpu1102I	GCTNAGC	1 cut(s)	600
Bpu14I	TTCGAA	2 cut(s)	102, 674
BpuMI	CCSGG	5 cut(s)	944, 1012, 1013, 1019, 1417
BsaAI	YACGTR	3 cut(s)	197, 426, 746
BsaHI	GRCGYC	1 cut(s)	934
BsaJI	CCNNGG	8 cut(s)	61, 249, 460, 461, 1011, 1080, 1158, 1189
BsaWI	WCCGGW	3 cut(s)	523, 718, 866
BsaXI	ACNNNNNCTCC	2 cut(s)	278, 308
Bse118I	RCCGGY	7 cut(s)	155, 188, 443, 616, 663, 755, 775
Bse1I	ACTGG	1 cut(s)	862
Bse3DI	GCAATG	1 cut(s)	761
BseAI	TCCGGA	1 cut(s)	523
BseBI	CCWGG	1 cut(s)	462
BseDI	CCNNGG	8 cut(s)	61, 249, 460, 461, 1011, 1080, 1158, 1189
BseGI	GGATG	2 cut(s)	1182, 1224
BseMI	GCAATG	1 cut(s)	761
BseMII	CTCAG	1 cut(s)	171
BseNI	ACTGG	1 cut(s)	862
BseSI	GKGCMC	3 cut(s)	830, 1088, 1379
BseX3I	CGGCCG	1 cut(s)	661
BseXI	GCAGC	4 cut(s)	252, 739, 1098, 1435
Bsh1236I	CGCG	7 cut(s)	38, 63, 221, 494, 967, 1202, 1355
Bsh1285I	CGRYCG	3 cut(s)	664, 723, 780
BshFI	GGCC	5 cut(s)	143, 260, 347, 663, 1415
BshNI	GGYRCC	1 cut(s)	827
BsiEI	CGRYCG	3 cut(s)	664, 723, 780
BsiHKAI	GWGCWC	4 cut(s)	74, 1140, 1379, 1411
BsiHKCI	CYCGRG	5 cut(s)	352, 407, 929, 1011, 1079
BsiWI	CGTACG	1 cut(s)	1368
BslFI	GGGAC	5 cut(s)	64, 226, 424, 1028, 1139
BsmAI	GTCTC	3 cut(s)	181, 238, 974
BsmBI	CGTCTC	2 cut(s)	181, 974
BsmFI	GGGAC	5 cut(s)	64, 226, 424, 1028, 1139
BsnI	GGCC	5 cut(s)	143, 260, 347, 663, 1415
BsoBI	CYCGRG	5 cut(s)	352, 407, 929, 1011, 1079
Bsp119I	TTCGAA	2 cut(s)	102, 674
Bsp1286I	GDGCHC	6 cut(s)	74, 830, 1088, 1140, 1379, 1411
Bsp13I	TCCGGA	1 cut(s)	523
Bsp143I	GATC	3 cut(s)	904, 939, 1038
Bsp1720I	GCTNAGC	1 cut(s)	600
Bsp19I	CCATGG	1 cut(s)	1189
BspANI	GGCC	5 cut(s)	143, 260, 347, 663, 1415
BspCNI	CTCAG	1 cut(s)	172
BspEI	TCCGGA	1 cut(s)	523
BspFNI	CGCG	7 cut(s)	38, 63, 221, 494, 967, 1202, 1355
BspHI	TCATGA	2 cut(s)	564, 1239
BspLI	GGNNCC	6 cut(s)	514, 829, 1016, 1155, 1195, 1224
BspPI	GGATC	2 cut(s)	899, 934
BspT104I	TTCGAA	2 cut(s)	102, 674
BspT107I	GGYRCC	1 cut(s)	827
BsrBI	CCGCTC	1 cut(s)	926
BsrDI	GCAATG	1 cut(s)	761
BsrFI	RCCGGY	7 cut(s)	155, 188, 443, 616, 663, 755, 775
BsrI	ACTGG	1 cut(s)	862
BssAI	RCCGGY	7 cut(s)	155, 188, 443, 616, 663, 755, 775
BssECI	CCNNGG	8 cut(s)	61, 249, 460, 461, 1011, 1080, 1158, 1189
BssMI	GATC	3 cut(s)	904, 939, 1038
BssNI	GRCGYC	1 cut(s)	934
BssSI	CACGAG	3 cut(s)	92, 203, 1387
BssT1I	CCWWGG	1 cut(s)	1189
Bst2BI	CACGAG	3 cut(s)	92, 203, 1387
Bst2UI	CCWGG	1 cut(s)	462
Bst4CI	ACNGT	3 cut(s)	31, 1000, 1432
Bst6I	CTCTTC	2 cut(s)	60, 881
BstACI	GRCGYC	1 cut(s)	934
BstAPI	GCANNNNNTGC	1 cut(s)	1092
BstBAI	YACGTR	3 cut(s)	197, 426, 746
BstBI	TTCGAA	2 cut(s)	102, 674
BstC8I	GCNNGC	9 cut(s)	153, 157, 190, 223, 665, 669, 739, 1309, 1360
BstDEI	CTNAG	3 cut(s)	180, 600, 846
BstDSI	CCRYGG	4 cut(s)	61, 249, 1158, 1189
BstEII	GGTNACC	2 cut(s)	539, 1061
BstF5I	GGATG	2 cut(s)	1182, 1224
BstFNI	CGCG	7 cut(s)	38, 63, 221, 494, 967, 1202, 1355
BstH2I	RGCGCY	1 cut(s)	396
BstHHI	GCGC	2 cut(s)	395, 496
BstKTI	GATC	3 cut(s)	907, 942, 1041
BstMAI	GTCTC	3 cut(s)	181, 238, 974
BstMBI	GATC	3 cut(s)	904, 939, 1038
BstMCI	CGRYCG	3 cut(s)	664, 723, 780
BstNI	CCWGG	1 cut(s)	462
BstNSI	RCATGY	2 cut(s)	1095, 1186
BstPI	GGTNACC	2 cut(s)	539, 1061
BstSCI	CCNGG	6 cut(s)	460, 942, 1010, 1011, 1017, 1415
BstSFI	CTRYAG	1 cut(s)	645
BstSLI	GKGCMC	3 cut(s)	830, 1088, 1379
BstSNI	TACGTA	1 cut(s)	746
BstUI	CGCG	7 cut(s)	38, 63, 221, 494, 967, 1202, 1355
BstV1I	GCAGC	4 cut(s)	252, 739, 1098, 1435
BstV2I	GAAGAC	1 cut(s)	304
BstZI	CGGCCG	1 cut(s)	661
BsuRI	GGCC	5 cut(s)	143, 260, 347, 663, 1415
BtgI	CCRYGG	4 cut(s)	61, 249, 1158, 1189
BtgZI	GCGATG	1 cut(s)	1178
BtsCI	GGATG	2 cut(s)	1182, 1224
BtsIMutI	CAGTG	2 cut(s)	27, 282
Cac8I	GCNNGC	9 cut(s)	153, 157, 190, 223, 665, 669, 739, 1309, 1360
CciI	TCATGA	2 cut(s)	564, 1239
CfoI	GCGC	2 cut(s)	395, 496
Cfr10I	RCCGGY	7 cut(s)	155, 188, 443, 616, 663, 755, 775
Cfr13I	GGNCC	4 cut(s)	258, 1015, 1153, 1222
Cfr42I	CCGCGG	1 cut(s)	64
Cfr9I	CCCGGG	1 cut(s)	1011
CseI	GACGC	5 cut(s)	500, 527, 923, 956, 1037
Csp6I	GTAC	6 cut(s)	82, 200, 427, 784, 1369, 1402
CviQI	GTAC	6 cut(s)	82, 200, 427, 784, 1369, 1402
DdeI	CTNAG	3 cut(s)	180, 600, 846
DpnI	GATC	3 cut(s)	906, 941, 1040
DpnII	GATC	3 cut(s)	904, 939, 1038
DrdI	GACNNNNNNGTC	2 cut(s)	32, 1151
DseDI	GACNNNNNNGTC	2 cut(s)	32, 1151
EaeI	YGGCCR	3 cut(s)	345, 661, 1413
EagI	CGGCCG	1 cut(s)	661
Eam1104I	CTCTTC	2 cut(s)	60, 881
EarI	CTCTTC	2 cut(s)	60, 881
EciI	GGCGGA	2 cut(s)	175, 1451
Ecl136II	GAGCTC	3 cut(s)	72, 1138, 1409
EclXI	CGGCCG	1 cut(s)	661
Eco105I	TACGTA	1 cut(s)	746
Eco130I	CCWWGG	1 cut(s)	1189
Eco24I	GRGCYC	3 cut(s)	74, 1140, 1411
Eco32I	GATATC	2 cut(s)	241, 529
Eco47I	GGWCC	3 cut(s)	1015, 1153, 1222
Eco52I	CGGCCG	1 cut(s)	661
Eco53kI	GAGCTC	3 cut(s)	72, 1138, 1409
Eco88I	CYCGRG	5 cut(s)	352, 407, 929, 1011, 1079
Eco91I	GGTNACC	2 cut(s)	539, 1061
EcoICRI	GAGCTC	3 cut(s)	72, 1138, 1409
EcoO65I	GGTNACC	2 cut(s)	539, 1061
EcoRII	CCWGG	1 cut(s)	460
EcoRV	GATATC	2 cut(s)	241, 529
EcoT14I	CCWWGG	1 cut(s)	1189
EcoT38I	GRGCYC	3 cut(s)	74, 1140, 1411
ErhI	CCWWGG	1 cut(s)	1189
Esp3I	CGTCTC	2 cut(s)	181, 974
FaqI	GGGAC	5 cut(s)	64, 226, 424, 1028, 1139
FauI	CCCGC	2 cut(s)	214, 317
FauNDI	CATATG	1 cut(s)	1073
FbaI	TGATCA	1 cut(s)	1038
Fnu4HI	GCNGC	6 cut(s)	112, 266, 753, 1112, 1356, 1449
FokI	GGATG	2 cut(s)	1189, 1231
FriOI	GRGCYC	3 cut(s)	74, 1140, 1411
Fsp4HI	GCNGC	6 cut(s)	112, 266, 753, 1112, 1356, 1449
FspBI	CTAG	5 cut(s)	1052, 1067, 1248, 1287, 1477
GlaI	GCGC	2 cut(s)	394, 495
GluI	GCNGC	6 cut(s)	112, 266, 753, 1112, 1356, 1449
GsuI	CTGGAG	1 cut(s)	825
HaeII	RGCGCY	1 cut(s)	396
HaeIII	GGCC	5 cut(s)	143, 260, 347, 663, 1415
HgaI	GACGC	5 cut(s)	500, 527, 923, 956, 1037
HhaI	GCGC	2 cut(s)	395, 496
Hin1I	GRCGYC	1 cut(s)	934
Hin6I	GCGC	2 cut(s)	393, 494
HinP1I	GCGC	2 cut(s)	393, 494
HincII	GTYRAC	2 cut(s)	855, 1296
HindII	GTYRAC	2 cut(s)	855, 1296
HindIII	AAGCTT	1 cut(s)	1046
HinfI	GANTC	8 cut(s)	52, 182, 245, 309, 357, 466, 534, 715
HpaI	GTTAAC	1 cut(s)	1296
HphI	GGTGA	6 cut(s)	138, 551, 631, 694, 978, 1073
Hpy166II	GTNNAC	6 cut(s)	200, 784, 855, 1296, 1377, 1386
Hpy188III	TCNNGA	8 cut(s)	211, 409, 524, 565, 1042, 1052, 1240, 1387
Hpy8I	GTNNAC	6 cut(s)	200, 784, 855, 1296, 1377, 1386
Hpy99I	CGWCG	5 cut(s)	322, 494, 521, 662, 1031
HpyAV	CCTTC	2 cut(s)	373, 1207
HpyCH4III	ACNGT	3 cut(s)	31, 1000, 1432
HpyCH4IV	ACGT	6 cut(s)	196, 425, 588, 745, 1268, 1382
HpyCH4V	TGCA	2 cut(s)	576, 1377
HpyF3I	CTNAG	3 cut(s)	180, 600, 846
HpySE526I	ACGT	6 cut(s)	196, 425, 588, 745, 1268, 1382
Hsp92I	GRCGYC	1 cut(s)	934
HspAI	GCGC	2 cut(s)	393, 494
Kpn2I	TCCGGA	1 cut(s)	523
KroI	GCCGGC	3 cut(s)	155, 188, 663
KroNI	GCCGGC	3 cut(s)	157, 190, 665
Ksp22I	TGATCA	1 cut(s)	1038
KspAI	GTTAAC	1 cut(s)	1296
KspI	CCGCGG	1 cut(s)	64
Kzo9I	GATC	3 cut(s)	904, 939, 1038
LmnI	GCTCC	3 cut(s)	69, 526, 1304
Lsp1109I	GCAGC	4 cut(s)	252, 739, 1098, 1435
MaeI	CTAG	5 cut(s)	1052, 1067, 1248, 1287, 1477
MaeII	ACGT	6 cut(s)	196, 425, 588, 745, 1268, 1382
MaeIII	GTNAC	4 cut(s)	539, 949, 1061, 1264
MalI	GATC	3 cut(s)	906, 941, 1040
MbiI	CCGCTC	1 cut(s)	926
MboI	GATC	3 cut(s)	904, 939, 1038
MboII	GAAGA	4 cut(s)	47, 304, 868, 1228
MhlI	GDGCHC	6 cut(s)	74, 830, 1088, 1140, 1379, 1411
MluCI	AATT	3 cut(s)	272, 632, 1464
MlyI	GAGTC	5 cut(s)	46, 191, 254, 318, 528
MmeI	TCCRAC	2 cut(s)	454, 540
MroI	TCCGGA	1 cut(s)	523
MroNI	GCCGGC	3 cut(s)	155, 188, 663
MroXI	GAANNNNTTC	1 cut(s)	1104
MseI	TTAA	2 cut(s)	498, 1295
MspA1I	CMGCKG	2 cut(s)	63, 1448
MspR9I	CCNGG	6 cut(s)	462, 944, 1012, 1013, 1019, 1417
MvaI	CCWGG	1 cut(s)	462
MvnI	CGCG	7 cut(s)	38, 63, 221, 494, 967, 1202, 1355
NaeI	GCCGGC	3 cut(s)	157, 190, 665
NciI	CCSGG	5 cut(s)	944, 1012, 1013, 1019, 1417
NcoI	CCATGG	1 cut(s)	1189
NdeI	CATATG	1 cut(s)	1073
NdeII	GATC	3 cut(s)	904, 939, 1038
NgoMIV	GCCGGC	3 cut(s)	155, 188, 663
NlaIV	GGNNCC	6 cut(s)	514, 829, 1016, 1155, 1195, 1224
NmeAIII	GCCGAG	2 cut(s)	1121, 1159
NmuCI	GTSAC	3 cut(s)	539, 949, 1061
NspI	RCATGY	2 cut(s)	1095, 1186
NspV	TTCGAA	2 cut(s)	102, 674
PagI	TCATGA	2 cut(s)	564, 1239
PasI	CCCWGGG	1 cut(s)	461
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 350
PdiI	GCCGGC	3 cut(s)	157, 190, 665
PdmI	GAANNNNTTC	1 cut(s)	1104
PfeI	GAWTC	3 cut(s)	357, 466, 715
Pfl23II	CGTACG	1 cut(s)	1368
PflFI	GACNNNGTC	1 cut(s)	1067
PflMI	CCANNNNNTGG	2 cut(s)	711, 1327
PkrI	GCNGC	6 cut(s)	113, 267, 754, 1113, 1357, 1450
PleI	GAGTC	5 cut(s)	46, 190, 253, 317, 528
PpsI	GAGTC	5 cut(s)	46, 190, 253, 317, 528
Ppu21I	YACGTR	3 cut(s)	197, 426, 746
Psp124BI	GAGCTC	3 cut(s)	74, 1140, 1411
Psp1406I	AACGTT	2 cut(s)	1268, 1382
Psp6I	CCWGG	1 cut(s)	460
PspEI	GGTNACC	2 cut(s)	539, 1061
PspGI	CCWGG	1 cut(s)	460
PspLI	CGTACG	1 cut(s)	1368
PspN4I	GGNNCC	6 cut(s)	514, 829, 1016, 1155, 1195, 1224
PspPI	GGNCC	4 cut(s)	258, 1015, 1153, 1222
PsyI	GACNNNGTC	1 cut(s)	1067
PvuII	CAGCTG	1 cut(s)	1448
RsaI	GTAC	6 cut(s)	83, 201, 428, 785, 1370, 1403
RsaNI	GTAC	6 cut(s)	82, 200, 427, 784, 1369, 1402
SacI	GAGCTC	3 cut(s)	74, 1140, 1411
SacII	CCGCGG	1 cut(s)	64
SaqAI	TTAA	2 cut(s)	498, 1295
SatI	GCNGC	6 cut(s)	112, 266, 753, 1112, 1356, 1449
Sau3AI	GATC	3 cut(s)	904, 939, 1038
Sau96I	GGNCC	4 cut(s)	258, 1015, 1153, 1222
SchI	GAGTC	5 cut(s)	46, 191, 254, 318, 528
ScrFI	CCNGG	6 cut(s)	462, 944, 1012, 1013, 1019, 1417
SduI	GDGCHC	6 cut(s)	74, 830, 1088, 1140, 1379, 1411
SfcI	CTRYAG	1 cut(s)	645
Sfr303I	CCGCGG	1 cut(s)	64
SfuI	TTCGAA	2 cut(s)	102, 674
SgrBI	CCGCGG	1 cut(s)	64
SinI	GGWCC	3 cut(s)	1015, 1153, 1222
SmaI	CCCGGG	1 cut(s)	1013
SnaBI	TACGTA	1 cut(s)	746
Sse9I	AATT	3 cut(s)	272, 632, 1464
SspMI	CTAG	5 cut(s)	1052, 1067, 1248, 1287, 1477
SstI	GAGCTC	3 cut(s)	74, 1140, 1411
StyD4I	CCNGG	6 cut(s)	460, 942, 1010, 1011, 1017, 1415
StyI	CCWWGG	1 cut(s)	1189
TaaI	ACNGT	3 cut(s)	31, 1000, 1432
TaiI	ACGT	6 cut(s)	199, 428, 591, 748, 1271, 1385
TaqI	TCGA	5 cut(s)	102, 243, 674, 903, 1316
TaqII	GACCGA	1 cut(s)	558
TasI	AATT	3 cut(s)	272, 632, 1464
TatI	WGTACW	3 cut(s)	199, 783, 1401
TauI	GCSGC	2 cut(s)	114, 1358
TfiI	GAWTC	3 cut(s)	357, 466, 715
Tru1I	TTAA	2 cut(s)	498, 1295
Tru9I	TTAA	2 cut(s)	498, 1295
TscAI	CASTG	2 cut(s)	34, 289
TseFI	GTSAC	3 cut(s)	539, 949, 1061
TseI	GCWGC	4 cut(s)	265, 752, 1111, 1448
Tsp45I	GTSAC	3 cut(s)	539, 949, 1061
TspDTI	ATGAA	8 cut(s)	242, 553, 581, 750, 868, 1228, 1256, 1275
TspGWI	ACGGA	6 cut(s)	69, 82, 238, 549, 675, 1157
TspMI	CCCGGG	1 cut(s)	1011
TspRI	CASTG	2 cut(s)	34, 289
Tth111I	GACNNNGTC	1 cut(s)	1067
Van91I	CCANNNNNTGG	2 cut(s)	711, 1327
VneI	GTGCAC	1 cut(s)	1375
VpaK11BI	GGWCC	3 cut(s)	1015, 1153, 1222
XapI	RAATTY	1 cut(s)	272
XbaI	TCTAGA	1 cut(s)	1051
XceI	RCATGY	2 cut(s)	1095, 1186
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1465
XmaI	CCCGGG	1 cut(s)	1011
XmnI	GAANNNNTTC	1 cut(s)	1104
XspI	CTAG	5 cut(s)	1052, 1067, 1248, 1287, 1477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.