Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Forward (+)

5721443 .. 5723681

Gene Structure 2239 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1081700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGACTACGACGACCTTCCCGACGGTTGATCAATGCACATCGGATGGACGGGAAAAGCACACAGTGGTCGCCGATATGGACGGGACTTTGCTTAGAGGCCGGAGCTCTTTCCCCTACTTTGCTCTCATTGCTTTTGAGGTTGGTGGGATACTAAGGCTCCTGTTCTTGATCTTGGTTACACCACTAGCCGGACTCCTCTACTACTTTGTCTCGGAATCAGCCGGAATCCAAGTTCTCATCTTTGCAACGTTTGCCGGGGTTAGGGTTTCGGAGATCGAGTCCGTGGCCAGAGCCGTGCTGCCTAAGTTTTACTCGAGTGATTTGCACCCGGAGTCTTGGCGCGTGATCTCTGCATGCGAAAAGAGATGTATTCTTACGGCAAATCCGAGGATCATGGTGGAGCCGTTTTTGAAGGATTTTCTTGGATTCGACACCGTTTTGGGGACGGAAGTGGCCACTTATAAGGGTAAGGCTACAGGGTTTGTTTCCCCACCTGGTGTGCTTGTGGGAAAGAACAAGGCAGATGCTCTTAAGAAGGCTTTTGGAGATGTCCTGCCAGAGATTGGGCTTGGTGATAGACACACTGACATTCCTTTCTTGAAACTGTGCAAGGAGGGTTACATGGTGCCAGCCAAACCAAAAGTGGAAGCAGTAACAAACGACAAGCTACCAAAGCCCATAATCTTCCACGACGGCCGCCTTGTTCAAAAGCCAACACCTCTCATGGCGCTCCTCACCCTCCTCTGGCTCCCCATCGGCTTCTTCCTCGCCTGCCTCCGTATTGCCGCCGGGGCGCTCCTCCCCATGCCGCTCGTCTATTACGCCTTCTGGGCCCTCGGCGTCCGTGTCACCGTCAAGGGCACCCCACCACCTGCCCTCAAAAAATCCACCAGCCACACCGGCGTCCTCTTCATCTGCTCCCACCGCACCCTCCTCGACCCCATCTTCCTCTCCACAGCCCTCGGCCGCCCCATCCCCGCCGTCACCTACTCCGTCTCTCGCCTCTCCGAGTTCATCTCCCCAATCAAGACCGTCCGCCTCAACCGCGATCGGGCCCTCGACGCCGCCATGATCAAGAAGCTTCTAGAAGAGGGTGACCTGGCAATCTGCCCGGAAGGCACCACGTGTCGAGAGCCCTTCCTCTTGAGATTCTCGGCGCTTTTCGCCGAGCTGACGGACGAACTGGTCCCGGTGGCGATGGTGAACAAGATGAGCATGTTTCACGGGACCACCGCACGTGGGTGGAAAGGGATGGACCCCTTCTACTTCTTCATGAACCCGAGCCCCGCGTACGAGGTGACGTTTTTGAACAAGCTGCCAGCTAAGCTGACGTATGGGGGCGGAAAGTCGAGCCATGAGGTGGCGAATTATATACAGAGGGTTATAGCGGCCAGCCTTTCGTATGAATGCACCGGGTTCACCAGGAAGGATAAGTACCGGGCTCTTGCCGGAAATGATGGGACGGTGACGACTGAGAAGCCGTTGCTCAAGCCCAATAAAATCATGGGATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

505

Amino Acids

55.35

Weight (kDa)

9.21

Isoelectric Point (pI)

37.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	21 - 209	7.8e-60	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	22 - 201	5.2e-26	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	298 - 399	1.4e-06	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	462
AarI	CACCTGC	1 cut(s)	880
AasI	GACNNNNNNGTC	1 cut(s)	1184
Acc36I	ACCTGC	1 cut(s)	880
AccB1I	GGYRCC	3 cut(s)	625, 860, 1118
AccB7I	CCANNNNNTGG	2 cut(s)	563, 1360
AccBSI	CCGCTC	1 cut(s)	811
AccII	CGCG	3 cut(s)	342, 1047, 1289
AclI	AACGTT	1 cut(s)	248
AclWI	GGATC	1 cut(s)	398
AcoI	YGGCCR	5 cut(s)	285, 453, 694, 964, 1389
AcvI	CACGTG	2 cut(s)	1125, 1238
AcyI	GRCGYC	3 cut(s)	840, 903, 1062
AdeI	CACNNNGTG	3 cut(s)	64, 497, 1125
AfaI	GTAC	2 cut(s)	1292, 1436
AfiI	CCNNNNNNNGG	8 cut(s)	188, 261, 441, 563, 744, 1051, 1239, 1360
AflII	CTTAAG	1 cut(s)	530
AflIII	ACRYGT	1 cut(s)	1124
AgsI	TTSAA	4 cut(s)	412, 601, 707, 1309
AjnI	CCWGG	3 cut(s)	493, 1098, 1421
AleI	CACNNNNGTG	1 cut(s)	1240
AloI	GAACNNNNNNTCC	2 cut(s)	216, 248
AluBI	AGCT	7 cut(s)	105, 667, 1081, 1171, 1315, 1322, 1327
AluI	AGCT	7 cut(s)	105, 667, 1081, 1171, 1315, 1322, 1327
Alw21I	GWGCWC	1 cut(s)	107
Alw26I	GTCTC	2 cut(s)	214, 1000
AlwI	GGATC	1 cut(s)	398
Ama87I	CYCGRG	2 cut(s)	313, 1279
AoxI	GGCC	8 cut(s)	97, 285, 453, 694, 831, 964, 1053, 1389
ApaI	GGGCCC	2 cut(s)	835, 1057
ApeKI	GCWGC	2 cut(s)	298, 1315
AspLEI	GCGC	4 cut(s)	342, 730, 796, 1159
AspS9I	GGNCC	7 cut(s)	831, 832, 1053, 1054, 1186, 1227, 1255
AsuC2I	CCSGG	7 cut(s)	256, 329, 790, 1112, 1190, 1414, 1439
AsuHPI	GGTGA	9 cut(s)	584, 727, 841, 976, 1106, 1213, 1309, 1411, 1477
AvaI	CYCGRG	2 cut(s)	313, 1279
AvaII	GGWCC	3 cut(s)	1186, 1227, 1255
BaeGI	GKGCMC	3 cut(s)	835, 863, 1057
BalI	TGGCCA	2 cut(s)	287, 455
BanI	GGYRCC	3 cut(s)	625, 860, 1118
BanII	GRGCYC	6 cut(s)	107, 835, 1057, 1137, 1286, 1444
BarI	GAAGNNNNNNTAC	2 cut(s)	1418, 1450
BbrPI	CACGTG	2 cut(s)	1125, 1238
Bbv12I	GWGCWC	1 cut(s)	107
BbvI	GCAGC	2 cut(s)	285, 1302
BccI	CCATC	7 cut(s)	38, 761, 950, 980, 1192, 1246, 1451
BceAI	ACGGC	6 cut(s)	278, 388, 393, 709, 965, 1465
BcgI	CGANNNNNNTGC	4 cut(s)	304, 338, 916, 950
BciT130I	CCWGG	3 cut(s)	495, 1100, 1423
BciVI	GTATCC	1 cut(s)	141
BclI	TGATCA	2 cut(s)	28, 1071
BcnI	CCSGG	7 cut(s)	256, 329, 790, 1112, 1190, 1414, 1439
BcoDI	GTCTC	2 cut(s)	214, 1000
BfaI	CTAG	3 cut(s)	185, 1085, 1513
BfmI	CTRYAG	1 cut(s)	474
BfoI	RGCGCY	3 cut(s)	731, 797, 1160
BfrI	CTTAAG	1 cut(s)	530
BfuAI	ACCTGC	1 cut(s)	880
BfuI	GTATCC	1 cut(s)	141
BglI	GCCNNNNNGGC	4 cut(s)	791, 830, 900, 1116
BisI	GCNGC	8 cut(s)	299, 697, 786, 809, 967, 1065, 1316, 1389
BlpI	GCTNAGC	1 cut(s)	1323
BlsI	GCNGC	8 cut(s)	300, 698, 787, 810, 968, 1066, 1317, 1390
Bme18I	GGWCC	3 cut(s)	1186, 1227, 1255
BmeT110I	CYCGRG	2 cut(s)	313, 1279
BmgT120I	GGNCC	7 cut(s)	831, 832, 1053, 1054, 1186, 1227, 1255
BmsI	GCATC	2 cut(s)	514, 1499
BplI	GAGNNNNNCTC	2 cut(s)	1001, 1033
Bpu1102I	GCTNAGC	1 cut(s)	1323
BpuEI	CTTGAG	2 cut(s)	1165, 1472
BpuMI	CCSGG	7 cut(s)	256, 329, 790, 1112, 1190, 1414, 1439
BsaAI	YACGTR	2 cut(s)	1125, 1238
BsaHI	GRCGYC	3 cut(s)	840, 903, 1062
BsaJI	CCNNGG	6 cut(s)	255, 282, 386, 789, 835, 961
BsaXI	ACNNNNNCTCC	4 cut(s)	141, 171, 263, 293
Bsc4I	CCNNNNNNNGG	8 cut(s)	188, 261, 441, 563, 744, 1051, 1239, 1360
Bse118I	RCCGGY	1 cut(s)	899
Bse1I	ACTGG	1 cut(s)	1188
Bse3DI	GCAATG	1 cut(s)	126
BseBI	CCWGG	3 cut(s)	495, 1100, 1423
BseDI	CCNNGG	6 cut(s)	255, 282, 386, 789, 835, 961
BseGI	GGATG	4 cut(s)	49, 972, 1257, 1514
BseLI	CCNNNNNNNGG	8 cut(s)	188, 261, 441, 563, 744, 1051, 1239, 1360
BseMI	GCAATG	1 cut(s)	126
BseMII	CTCAG	1 cut(s)	1464
BseNI	ACTGG	1 cut(s)	1188
BseRI	GAGGAG	5 cut(s)	185, 722, 731, 788, 923
BseSI	GKGCMC	3 cut(s)	835, 863, 1057
BseX3I	CGGCCG	2 cut(s)	694, 964
BseXI	GCAGC	2 cut(s)	285, 1302
Bsh1236I	CGCG	3 cut(s)	342, 1047, 1289
Bsh1285I	CGRYCG	3 cut(s)	697, 967, 1051
BshFI	GGCC	8 cut(s)	99, 287, 455, 696, 833, 966, 1055, 1391
BshNI	GGYRCC	3 cut(s)	625, 860, 1118
BsiEI	CGRYCG	3 cut(s)	697, 967, 1051
BsiHKAI	GWGCWC	1 cut(s)	107
BsiHKCI	CYCGRG	2 cut(s)	313, 1279
BsiWI	CGTACG	1 cut(s)	1290
BslFI	GGGAC	5 cut(s)	97, 457, 1172, 1240, 1474
BslI	CCNNNNNNNGG	8 cut(s)	188, 261, 441, 563, 744, 1051, 1239, 1360
BsmAI	GTCTC	2 cut(s)	214, 1000
BsmBI	CGTCTC	1 cut(s)	1000
BsmFI	GGGAC	5 cut(s)	97, 457, 1172, 1240, 1474
BsmI	GAATGC	1 cut(s)	1412
BsnI	GGCC	8 cut(s)	99, 287, 455, 696, 833, 966, 1055, 1391
BsoBI	CYCGRG	2 cut(s)	313, 1279
Bsp120I	GGGCCC	2 cut(s)	831, 1053
Bsp1286I	GDGCHC	7 cut(s)	107, 835, 863, 1057, 1137, 1286, 1444
Bsp143I	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
Bsp1720I	GCTNAGC	1 cut(s)	1323
BspANI	GGCC	8 cut(s)	99, 287, 455, 696, 833, 966, 1055, 1391
BspCNI	CTCAG	1 cut(s)	1465
BspFNI	CGCG	3 cut(s)	342, 1047, 1289
BspHI	TCATGA	1 cut(s)	1272
BspMI	ACCTGC	1 cut(s)	880
BspPI	GGATC	1 cut(s)	398
BspT107I	GGYRCC	3 cut(s)	625, 860, 1118
BspTI	CTTAAG	1 cut(s)	530
BsrBI	CCGCTC	1 cut(s)	811
BsrDI	GCAATG	1 cut(s)	126
BsrFI	RCCGGY	1 cut(s)	899
BsrI	ACTGG	1 cut(s)	1188
BssAI	RCCGGY	1 cut(s)	899
BssECI	CCNNGG	6 cut(s)	255, 282, 386, 789, 835, 961
BssMI	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
BssNI	GRCGYC	3 cut(s)	840, 903, 1062
Bst2UI	CCWGG	3 cut(s)	495, 1100, 1423
Bst4CI	ACNGT	7 cut(s)	25, 64, 436, 606, 853, 1033, 1465
Bst6I	CTCTTC	2 cut(s)	914, 1083
BstACI	GRCGYC	3 cut(s)	840, 903, 1062
BstAFI	CTTAAG	1 cut(s)	530
BstAPI	GCANNNNNTGC	1 cut(s)	251
BstBAI	YACGTR	2 cut(s)	1125, 1238
BstC8I	GCNNGC	5 cut(s)	355, 630, 772, 1320, 1393
BstDEI	CTNAG	5 cut(s)	92, 152, 303, 1323, 1473
BstDSI	CCRYGG	1 cut(s)	282
BstEII	GGTNACC	1 cut(s)	1094
BstF5I	GGATG	4 cut(s)	49, 972, 1257, 1514
BstFNI	CGCG	3 cut(s)	342, 1047, 1289
BstH2I	RGCGCY	3 cut(s)	731, 797, 1160
BstHHI	GCGC	4 cut(s)	342, 730, 796, 1159
BstKTI	GATC	7 cut(s)	31, 171, 276, 348, 393, 1051, 1074
BstMAI	GTCTC	2 cut(s)	214, 1000
BstMBI	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
BstMCI	CGRYCG	3 cut(s)	697, 967, 1051
BstNI	CCWGG	3 cut(s)	495, 1100, 1423
BstNSI	RCATGY	2 cut(s)	357, 1219
BstPI	GGTNACC	1 cut(s)	1094
BstSFI	CTRYAG	1 cut(s)	474
BstSLI	GKGCMC	3 cut(s)	835, 863, 1057
BstUI	CGCG	3 cut(s)	342, 1047, 1289
BstV1I	GCAGC	2 cut(s)	285, 1302
BstZI	CGGCCG	2 cut(s)	694, 964
BsuI	GTATCC	1 cut(s)	141
BsuRI	GGCC	8 cut(s)	99, 287, 455, 696, 833, 966, 1055, 1391
BtgI	CCRYGG	1 cut(s)	282
BtgZI	GCGATG	1 cut(s)	1211
BtsCI	GGATG	4 cut(s)	49, 972, 1257, 1514
BtsIMutI	CAGTG	2 cut(s)	69, 582
BveI	ACCTGC	1 cut(s)	880
Cac8I	GCNNGC	5 cut(s)	355, 630, 772, 1320, 1393
CciI	TCATGA	1 cut(s)	1272
CfoI	GCGC	4 cut(s)	342, 730, 796, 1159
Cfr10I	RCCGGY	1 cut(s)	899
Cfr13I	GGNCC	7 cut(s)	831, 832, 1053, 1054, 1186, 1227, 1255
CseI	GACGC	3 cut(s)	829, 892, 1070
CsiI	ACCWGGT	1 cut(s)	493
Csp6I	GTAC	2 cut(s)	1291, 1435
CviQI	GTAC	2 cut(s)	1291, 1435
DdeI	CTNAG	5 cut(s)	92, 152, 303, 1323, 1473
DpnI	GATC	7 cut(s)	30, 170, 275, 347, 392, 1050, 1073
DpnII	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
DraIII	CACNNNGTG	3 cut(s)	64, 497, 1125
DrdI	GACNNNNNNGTC	1 cut(s)	1184
DseDI	GACNNNNNNGTC	1 cut(s)	1184
EaeI	YGGCCR	5 cut(s)	285, 453, 694, 964, 1389
EagI	CGGCCG	2 cut(s)	694, 964
Eam1104I	CTCTTC	2 cut(s)	914, 1083
EarI	CTCTTC	2 cut(s)	914, 1083
EciI	GGCGGA	2 cut(s)	1025, 1356
Ecl136II	GAGCTC	1 cut(s)	105
EclXI	CGGCCG	2 cut(s)	694, 964
Eco24I	GRGCYC	6 cut(s)	107, 835, 1057, 1137, 1286, 1444
Eco47I	GGWCC	3 cut(s)	1186, 1227, 1255
Eco52I	CGGCCG	2 cut(s)	694, 964
Eco53kI	GAGCTC	1 cut(s)	105
Eco72I	CACGTG	2 cut(s)	1125, 1238
Eco88I	CYCGRG	2 cut(s)	313, 1279
Eco91I	GGTNACC	1 cut(s)	1094
EcoICRI	GAGCTC	1 cut(s)	105
EcoO109I	RGGNCCY	2 cut(s)	832, 1054
EcoO65I	GGTNACC	1 cut(s)	1094
EcoRII	CCWGG	3 cut(s)	493, 1098, 1421
EcoT38I	GRGCYC	6 cut(s)	107, 835, 1057, 1137, 1286, 1444
Esp3I	CGTCTC	1 cut(s)	1000
FaqI	GGGAC	5 cut(s)	97, 457, 1172, 1240, 1474
FauI	CCCGC	2 cut(s)	985, 1294
FbaI	TGATCA	2 cut(s)	28, 1071
Fnu4HI	GCNGC	8 cut(s)	299, 697, 786, 809, 967, 1065, 1316, 1389
FokI	GGATG	3 cut(s)	56, 959, 1264
FriOI	GRGCYC	6 cut(s)	107, 835, 1057, 1137, 1286, 1444
Fsp4HI	GCNGC	8 cut(s)	299, 697, 786, 809, 967, 1065, 1316, 1389
FspBI	CTAG	3 cut(s)	185, 1085, 1513
GlaI	GCGC	4 cut(s)	341, 729, 795, 1158
GluI	GCNGC	8 cut(s)	299, 697, 786, 809, 967, 1065, 1316, 1389
HaeII	RGCGCY	3 cut(s)	731, 797, 1160
HaeIII	GGCC	8 cut(s)	99, 287, 455, 696, 833, 966, 1055, 1391
HgaI	GACGC	3 cut(s)	829, 892, 1070
HhaI	GCGC	4 cut(s)	342, 730, 796, 1159
Hin1I	GRCGYC	3 cut(s)	840, 903, 1062
Hin6I	GCGC	4 cut(s)	340, 728, 794, 1157
HinP1I	GCGC	4 cut(s)	340, 728, 794, 1157
HindIII	AAGCTT	1 cut(s)	1079
HinfI	GANTC	7 cut(s)	192, 215, 225, 278, 332, 426, 1149
HphI	GGTGA	9 cut(s)	584, 727, 841, 976, 1106, 1213, 1309, 1411, 1477
Hpy166II	GTNNAC	2 cut(s)	1204, 1419
Hpy188I	TCNGA	5 cut(s)	43, 214, 271, 387, 1009
Hpy188III	TCNNGA	9 cut(s)	19, 166, 598, 1027, 1075, 1085, 1130, 1144, 1273
Hpy8I	GTNNAC	2 cut(s)	1204, 1419
Hpy99I	CGWCG	4 cut(s)	13, 25, 695, 1064
HpyAV	CCTTC	8 cut(s)	25, 406, 529, 835, 1109, 1147, 1270, 1420
HpyCH4III	ACNGT	7 cut(s)	25, 64, 436, 606, 853, 1033, 1465
HpyCH4IV	ACGT	5 cut(s)	248, 1124, 1237, 1301, 1331
HpyCH4V	TGCA	6 cut(s)	36, 245, 325, 353, 609, 1410
HpyF3I	CTNAG	5 cut(s)	92, 152, 303, 1323, 1473
HpySE526I	ACGT	5 cut(s)	248, 1124, 1237, 1301, 1331
Hsp92I	GRCGYC	3 cut(s)	840, 903, 1062
HspAI	GCGC	4 cut(s)	340, 728, 794, 1157
Ksp22I	TGATCA	2 cut(s)	28, 1071
Kzo9I	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
LmnI	GCTCC	7 cut(s)	102, 162, 400, 735, 753, 801, 923
Lsp1109I	GCAGC	2 cut(s)	285, 1302
LweI	GCATC	2 cut(s)	514, 1499
MabI	ACCWGGT	1 cut(s)	493
MaeI	CTAG	3 cut(s)	185, 1085, 1513
MaeII	ACGT	5 cut(s)	248, 1124, 1237, 1301, 1331
MaeIII	GTNAC	8 cut(s)	175, 617, 652, 847, 982, 1094, 1297, 1465
MalI	GATC	7 cut(s)	30, 170, 275, 347, 392, 1050, 1073
MbiI	CCGCTC	1 cut(s)	811
MboI	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
MboII	GAAGA	6 cut(s)	676, 754, 901, 937, 1100, 1261
MhlI	GDGCHC	7 cut(s)	107, 835, 863, 1057, 1137, 1286, 1444
MlsI	TGGCCA	2 cut(s)	287, 455
MluCI	AATT	1 cut(s)	1366
MluNI	TGGCCA	2 cut(s)	287, 455
MlyI	GAGTC	3 cut(s)	186, 287, 341
Mox20I	TGGCCA	2 cut(s)	287, 455
MscI	TGGCCA	2 cut(s)	287, 455
MseI	TTAA	1 cut(s)	531
MslI	CAYNNNNRTG	1 cut(s)	1240
Msp20I	TGGCCA	2 cut(s)	287, 455
MspCI	CTTAAG	1 cut(s)	530
Mva1269I	GAATGC	1 cut(s)	1412
MvaI	CCWGG	3 cut(s)	495, 1100, 1423
MvnI	CGCG	3 cut(s)	342, 1047, 1289
NciI	CCSGG	7 cut(s)	256, 329, 790, 1112, 1190, 1414, 1439
NdeII	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
NmeAIII	GCCGAG	4 cut(s)	816, 942, 1133, 1192
NmuCI	GTSAC	5 cut(s)	847, 982, 1094, 1297, 1465
NspI	RCATGY	2 cut(s)	357, 1219
OliI	CACNNNNGTG	1 cut(s)	1240
PaeI	GCATGC	1 cut(s)	357
PaeR7I	CTCGAG	1 cut(s)	313
PagI	TCATGA	1 cut(s)	1272
PaqCI	CACCTGC	1 cut(s)	880
PcsI	WCGNNNNNNNCGW	2 cut(s)	17, 383
PctI	GAATGC	1 cut(s)	1412
PfeI	GAWTC	4 cut(s)	215, 225, 426, 1149
Pfl23II	CGTACG	1 cut(s)	1290
PflMI	CCANNNNNTGG	2 cut(s)	563, 1360
PkrI	GCNGC	8 cut(s)	300, 698, 787, 810, 968, 1066, 1317, 1390
Ple19I	CGATCG	1 cut(s)	1051
PleI	GAGTC	3 cut(s)	186, 286, 340
PmaCI	CACGTG	2 cut(s)	1125, 1238
PmlI	CACGTG	2 cut(s)	1125, 1238
PpsI	GAGTC	3 cut(s)	186, 286, 340
Ppu21I	YACGTR	2 cut(s)	1125, 1238
PsiI	TTATAA	1 cut(s)	462
Psp124BI	GAGCTC	1 cut(s)	107
Psp1406I	AACGTT	1 cut(s)	248
Psp6I	CCWGG	3 cut(s)	493, 1098, 1421
PspCI	CACGTG	2 cut(s)	1125, 1238
PspEI	GGTNACC	1 cut(s)	1094
PspGI	CCWGG	3 cut(s)	493, 1098, 1421
PspLI	CGTACG	1 cut(s)	1290
PspOMI	GGGCCC	2 cut(s)	831, 1053
PspPI	GGNCC	7 cut(s)	831, 832, 1053, 1054, 1186, 1227, 1255
PspXI	VCTCGAGB	1 cut(s)	313
PvuI	CGATCG	1 cut(s)	1051
RsaI	GTAC	2 cut(s)	1292, 1436
RsaNI	GTAC	2 cut(s)	1291, 1435
RseI	CAYNNNNRTG	1 cut(s)	1240
SacI	GAGCTC	1 cut(s)	107
SaqAI	TTAA	1 cut(s)	531
SatI	GCNGC	8 cut(s)	299, 697, 786, 809, 967, 1065, 1316, 1389
Sau3AI	GATC	7 cut(s)	28, 168, 273, 345, 390, 1048, 1071
Sau96I	GGNCC	7 cut(s)	831, 832, 1053, 1054, 1186, 1227, 1255
SchI	GAGTC	3 cut(s)	186, 287, 341
SduI	GDGCHC	7 cut(s)	107, 835, 863, 1057, 1137, 1286, 1444
SexAI	ACCWGGT	1 cut(s)	493
SfaNI	GCATC	2 cut(s)	514, 1499
SfcI	CTRYAG	1 cut(s)	474
Sfr274I	CTCGAG	1 cut(s)	313
SgrAI	CRCCGGYG	1 cut(s)	899
SinI	GGWCC	3 cut(s)	1186, 1227, 1255
SlaI	CTCGAG	1 cut(s)	313
SmiMI	CAYNNNNRTG	1 cut(s)	1240
SmlI	CTYRAG	4 cut(s)	313, 530, 1144, 1487
SmoI	CTYRAG	4 cut(s)	313, 530, 1144, 1487
SphI	GCATGC	1 cut(s)	357
Sse9I	AATT	1 cut(s)	1366
SspMI	CTAG	3 cut(s)	185, 1085, 1513
SstI	GAGCTC	1 cut(s)	107
TaaI	ACNGT	7 cut(s)	25, 64, 436, 606, 853, 1033, 1465
TaiI	ACGT	5 cut(s)	251, 1127, 1240, 1304, 1334
TaqI	TCGA	7 cut(s)	276, 314, 429, 936, 1059, 1129, 1349
TasI	AATT	1 cut(s)	1366
TauI	GCSGC	6 cut(s)	699, 788, 811, 969, 1067, 1391
TfiI	GAWTC	4 cut(s)	215, 225, 426, 1149
Tru1I	TTAA	1 cut(s)	531
Tru9I	TTAA	1 cut(s)	531
TscAI	CASTG	2 cut(s)	69, 589
TseFI	GTSAC	5 cut(s)	847, 982, 1094, 1297, 1465
TseI	GCWGC	2 cut(s)	298, 1315
Tsp45I	GTSAC	5 cut(s)	847, 982, 1094, 1297, 1465
TspDTI	ATGAA	5 cut(s)	901, 1003, 1261, 1289, 1419
TspGWI	ACGGA	6 cut(s)	271, 461, 767, 833, 982, 1190
TspRI	CASTG	2 cut(s)	69, 589
Van91I	CCANNNNNTGG	2 cut(s)	563, 1360
Vha464I	CTTAAG	1 cut(s)	530
VpaK11BI	GGWCC	3 cut(s)	1186, 1227, 1255
XbaI	TCTAGA	1 cut(s)	1084
XceI	RCATGY	2 cut(s)	357, 1219
XcmI	CCANNNNNNNNNTGG	2 cut(s)	640, 1501
XhoI	CTCGAG	1 cut(s)	313
XspI	CTAG	3 cut(s)	185, 1085, 1513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.