Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Forward (+)

83958278 .. 83959756

Gene Structure 1479 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG614100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGGACGGGACTCTTCTCCGCGGACGGAGCTCATTTCCGTACTTCGCCCTCGTGGCTTTCGAAGTTGGCGGCATTTTGAGGCTTCTTTTTCTGCTCTTGGCCTCACCGCTTGCCGGCGTTCTCTACTACTTCGTCTCTGAGTCCGCCGGCATACGTGTACTCGTGTTCGGGACTTTCGCGGGCATGAAAGTCGCGGATATCGAGTCCGTGGCACGCGCCGTGCTGCCGAAATTCTACTCCACTGACTTGCACTCCGAAACATGGAGAGTCTTCTCGTCGTGCGGGAAACGCTGTGTTTTGACGGCCAACCCGAGAATCATGGTGGAGGGGTTTTTGAAGGAGTTTTTGGGCGCTGACATGGTTCTCGGGACAGAGATTGCCACGTACAGAGGTAGAGCTACCGGCTTTGTCCTAAACCCTGGGATTCTTGTCGGAAACAACAAAGCCGACGCGCTTAAAAAAGCGTTTGGTTCCGACGCTCCGGATATCGGACTCGGTGACCGAAAAACTGACTTTCCGTTCATGACGCTGTGCAAAGAGAGCTACGTTGTTCCGGCTAAGCCGGAAGTTGAGCCGGTGAGCCACGAAAAATTACCGAAGCCTATAGTGTTCCACGACGGCCGGCTCGCTTCGAAACTAACTCCGTTGATGGCTTTGCTCACCATTCTTTGGATTCCGGTCGGTTTCGTTTTGGCTTGCTTACGTATTGCTGCCGGTTCATTGCTTCCTATGCCGGTCGTGTACTATGCATTTTGGGCTTTGGGTGTTAGAGTTTATGTCAAGGGCACCCCACCACCTCCAGCTAAGAAGTCAACGGGTCAAACTGGAGTTCTCTTCATTTGCTCACACCGAACCCTACTCGATCCCATTTTCCTCTCCACCGCTCTCGGGCGTCCGATCCCGGCAGTCACATACTCTCTCTCGCGTCTCTCGGAGATAATCTCGCCCATCAAAACCGTCCGGCTAACCCGGGACCGGGTTGCCGACGCCAACATGATCAAGAAGCTTCTAGAACAAGGTGACCTAGTCATATGCCCCGAGGGCACAACATGCCGAGAACCCTTTCTGCTGCGGTTCTCTGCGTTGTTTGCTGAGCTCACGGACGAGCTGGTCCCCGTGGCGATGGCAAATCGGATGAGCATGTTCCATGGAACCACCGCGAGGGGGTGGAAGGGGATGGACCCGTTTTACTTCTTCATGAACCCTAGCCCTGCTTATGAAGTAACGTTTTTGAACAAGTTGCCTAGCGAGTTAACTTGCGGAGCAGGCAAGTCGAGCCATGATGTGGCGAACTATATACAAAGGACTATCGCGGCGAGCTTGTCGTACGAGTGCACAACGTTCACGAGGAAAGACAAGTACAGAGCTCTCGCCGGGAATGATGGCACGGTGGCGGAGAAACCAGCTGCTAATGCCAAAAAAATTATGGGGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

478

Amino Acids

52.21

Weight (kDa)

9.26

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	1 - 184	4e-60	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	1 - 175	9.6e-22	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	274 - 373	1.8e-06	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1109
AccB1I	GGYRCC	1 cut(s)	785
AccB7I	CCANNNNNTGG	2 cut(s)	669, 1285
AccBSI	CCGCTC	1 cut(s)	884
AccII	CGCG	8 cut(s)	21, 179, 194, 216, 452, 925, 1160, 1313
AccIII	TCCGGA	1 cut(s)	481
AclI	AACGTT	2 cut(s)	1226, 1340
AclWI	GGATC	2 cut(s)	857, 892
AcoI	YGGCCR	2 cut(s)	303, 619
AcsI	RAATTY	1 cut(s)	230
AcyI	GRCGYC	2 cut(s)	892, 987
AfaI	GTAC	6 cut(s)	41, 159, 386, 743, 1328, 1361
AflIII	ACRYGT	1 cut(s)	154
AgsI	TTSAA	2 cut(s)	337, 1234
AjnI	CCWGG	1 cut(s)	418
AjuI	GAANNNNNNNTTGG	2 cut(s)	329, 361
Alw21I	GWGCWC	4 cut(s)	32, 1098, 1337, 1369
Alw26I	GTCTC	2 cut(s)	139, 932
Alw44I	GTGCAC	1 cut(s)	1333
AlwI	GGATC	2 cut(s)	857, 892
Ama87I	CYCGRG	5 cut(s)	310, 365, 887, 969, 1037
Aor13HI	TCCGGA	1 cut(s)	481
AoxI	GGCC	3 cut(s)	99, 303, 619
ApaLI	GTGCAC	1 cut(s)	1333
ApeKI	GCWGC	4 cut(s)	223, 710, 1069, 1406
ApoI	RAATTY	1 cut(s)	230
Asp700I	GAANNNNTTC	1 cut(s)	1062
AspLEI	GCGC	3 cut(s)	218, 353, 454
AspS9I	GGNCC	3 cut(s)	973, 1111, 1180
AsuC2I	CCSGG	5 cut(s)	902, 970, 971, 977, 1375
AsuHPI	GGTGA	5 cut(s)	96, 509, 589, 652, 1031
AsuII	TTCGAA	2 cut(s)	60, 632
AvaI	CYCGRG	5 cut(s)	310, 365, 887, 969, 1037
AvaII	GGWCC	3 cut(s)	973, 1111, 1180
BaeGI	GKGCMC	3 cut(s)	788, 1046, 1337
BanI	GGYRCC	1 cut(s)	785
BanII	GRGCYC	3 cut(s)	32, 1098, 1369
BauI	CACGAG	3 cut(s)	50, 161, 1345
BbsI	GAAGAC	1 cut(s)	262
Bbv12I	GWGCWC	4 cut(s)	32, 1098, 1337, 1369
BbvI	GCAGC	4 cut(s)	210, 697, 1056, 1393
BccI	CCATC	5 cut(s)	643, 956, 1117, 1171, 1376
BceAI	ACGGC	3 cut(s)	203, 318, 634
BcgI	CGANNNNNNTGC	4 cut(s)	172, 206, 1254, 1288
BciT130I	CCWGG	1 cut(s)	420
BclI	TGATCA	1 cut(s)	996
BcnI	CCSGG	5 cut(s)	902, 970, 971, 977, 1375
BcoDI	GTCTC	2 cut(s)	139, 932
BfaI	CTAG	5 cut(s)	1010, 1025, 1206, 1245, 1435
BfmI	CTRYAG	1 cut(s)	603
BfoI	RGCGCY	1 cut(s)	354
BglI	GCCNNNNNGGC	2 cut(s)	53, 1041
BisI	GCNGC	6 cut(s)	70, 224, 711, 1070, 1314, 1407
BlpI	GCTNAGC	2 cut(s)	558, 1092
BlsI	GCNGC	6 cut(s)	71, 225, 712, 1071, 1315, 1408
Bme1390I	CCNGG	6 cut(s)	420, 902, 970, 971, 977, 1375
Bme18I	GGWCC	3 cut(s)	973, 1111, 1180
BmeT110I	CYCGRG	5 cut(s)	310, 365, 887, 969, 1037
BmgT120I	GGNCC	3 cut(s)	973, 1111, 1180
BmiI	GGNNCC	6 cut(s)	472, 787, 974, 1113, 1153, 1182
BmrFI	CCNGG	6 cut(s)	420, 902, 970, 971, 977, 1375
BpiI	GAAGAC	1 cut(s)	262
BplI	GAGNNNNNCTC	2 cut(s)	926, 958
BpmI	CTGGAG	2 cut(s)	783, 846
Bpu1102I	GCTNAGC	2 cut(s)	558, 1092
Bpu14I	TTCGAA	2 cut(s)	60, 632
BpuMI	CCSGG	5 cut(s)	902, 970, 971, 977, 1375
BsaAI	YACGTR	3 cut(s)	155, 384, 704
BsaHI	GRCGYC	2 cut(s)	892, 987
BsaJI	CCNNGG	8 cut(s)	19, 207, 418, 419, 969, 1038, 1116, 1147
BsaWI	WCCGGW	2 cut(s)	481, 676
BsaXI	ACNNNNNCTCC	2 cut(s)	236, 266
Bse118I	RCCGGY	7 cut(s)	113, 146, 401, 574, 621, 713, 733
Bse1I	ACTGG	1 cut(s)	829
Bse3DI	GCAATG	1 cut(s)	719
BseAI	TCCGGA	1 cut(s)	481
BseBI	CCWGG	1 cut(s)	420
BseDI	CCNNGG	8 cut(s)	19, 207, 418, 419, 969, 1038, 1116, 1147
BseGI	GGATG	2 cut(s)	1140, 1182
BseMI	GCAATG	1 cut(s)	719
BseMII	CTCAG	2 cut(s)	129, 1083
BseNI	ACTGG	1 cut(s)	829
BseSI	GKGCMC	3 cut(s)	788, 1046, 1337
BseX3I	CGGCCG	1 cut(s)	619
BseXI	GCAGC	4 cut(s)	210, 697, 1056, 1393
Bsh1236I	CGCG	8 cut(s)	21, 179, 194, 216, 452, 925, 1160, 1313
Bsh1285I	CGRYCG	3 cut(s)	622, 681, 738
BshFI	GGCC	3 cut(s)	101, 305, 621
BshNI	GGYRCC	1 cut(s)	785
BsiEI	CGRYCG	3 cut(s)	622, 681, 738
BsiHKAI	GWGCWC	4 cut(s)	32, 1098, 1337, 1369
BsiHKCI	CYCGRG	5 cut(s)	310, 365, 887, 969, 1037
BsiWI	CGTACG	1 cut(s)	1326
BslFI	GGGAC	5 cut(s)	22, 184, 382, 986, 1097
BsmAI	GTCTC	2 cut(s)	139, 932
BsmBI	CGTCTC	2 cut(s)	139, 932
BsmFI	GGGAC	5 cut(s)	22, 184, 382, 986, 1097
BsnI	GGCC	3 cut(s)	101, 305, 621
BsoBI	CYCGRG	5 cut(s)	310, 365, 887, 969, 1037
Bsp119I	TTCGAA	2 cut(s)	60, 632
Bsp1286I	GDGCHC	6 cut(s)	32, 788, 1046, 1098, 1337, 1369
Bsp13I	TCCGGA	1 cut(s)	481
Bsp143I	GATC	3 cut(s)	862, 897, 996
Bsp1720I	GCTNAGC	2 cut(s)	558, 1092
Bsp19I	CCATGG	1 cut(s)	1147
BspANI	GGCC	3 cut(s)	101, 305, 621
BspCNI	CTCAG	2 cut(s)	130, 1084
BspEI	TCCGGA	1 cut(s)	481
BspFNI	CGCG	8 cut(s)	21, 179, 194, 216, 452, 925, 1160, 1313
BspHI	TCATGA	2 cut(s)	522, 1197
BspLI	GGNNCC	6 cut(s)	472, 787, 974, 1113, 1153, 1182
BspPI	GGATC	2 cut(s)	857, 892
BspT104I	TTCGAA	2 cut(s)	60, 632
BspT107I	GGYRCC	1 cut(s)	785
BsrBI	CCGCTC	1 cut(s)	884
BsrDI	GCAATG	1 cut(s)	719
BsrFI	RCCGGY	7 cut(s)	113, 146, 401, 574, 621, 713, 733
BsrI	ACTGG	1 cut(s)	829
BssAI	RCCGGY	7 cut(s)	113, 146, 401, 574, 621, 713, 733
BssECI	CCNNGG	8 cut(s)	19, 207, 418, 419, 969, 1038, 1116, 1147
BssMI	GATC	3 cut(s)	862, 897, 996
BssNI	GRCGYC	2 cut(s)	892, 987
BssSI	CACGAG	3 cut(s)	50, 161, 1345
BssT1I	CCWWGG	1 cut(s)	1147
Bst2BI	CACGAG	3 cut(s)	50, 161, 1345
Bst2UI	CCWGG	1 cut(s)	420
Bst4CI	ACNGT	2 cut(s)	958, 1390
Bst6I	CTCTTC	2 cut(s)	18, 839
BstACI	GRCGYC	2 cut(s)	892, 987
BstAPI	GCANNNNNTGC	1 cut(s)	1050
BstBAI	YACGTR	3 cut(s)	155, 384, 704
BstBI	TTCGAA	2 cut(s)	60, 632
BstDEI	CTNAG	4 cut(s)	138, 558, 804, 1092
BstDSI	CCRYGG	4 cut(s)	19, 207, 1116, 1147
BstEII	GGTNACC	2 cut(s)	497, 1019
BstF5I	GGATG	2 cut(s)	1140, 1182
BstFNI	CGCG	8 cut(s)	21, 179, 194, 216, 452, 925, 1160, 1313
BstH2I	RGCGCY	1 cut(s)	354
BstHHI	GCGC	3 cut(s)	218, 353, 454
BstKTI	GATC	3 cut(s)	865, 900, 999
BstMAI	GTCTC	2 cut(s)	139, 932
BstMBI	GATC	3 cut(s)	862, 897, 996
BstMCI	CGRYCG	3 cut(s)	622, 681, 738
BstNI	CCWGG	1 cut(s)	420
BstNSI	RCATGY	2 cut(s)	1053, 1144
BstPI	GGTNACC	2 cut(s)	497, 1019
BstSCI	CCNGG	6 cut(s)	418, 900, 968, 969, 975, 1373
BstSFI	CTRYAG	1 cut(s)	603
BstSLI	GKGCMC	3 cut(s)	788, 1046, 1337
BstSNI	TACGTA	1 cut(s)	704
BstUI	CGCG	8 cut(s)	21, 179, 194, 216, 452, 925, 1160, 1313
BstV1I	GCAGC	4 cut(s)	210, 697, 1056, 1393
BstV2I	GAAGAC	1 cut(s)	262
BstZI	CGGCCG	1 cut(s)	619
BsuRI	GGCC	3 cut(s)	101, 305, 621
BtgI	CCRYGG	4 cut(s)	19, 207, 1116, 1147
BtgZI	GCGATG	1 cut(s)	1136
BtsCI	GGATG	2 cut(s)	1140, 1182
BtsIMutI	CAGTG	1 cut(s)	240
CciI	TCATGA	2 cut(s)	522, 1197
CfoI	GCGC	3 cut(s)	218, 353, 454
Cfr10I	RCCGGY	7 cut(s)	113, 146, 401, 574, 621, 713, 733
Cfr13I	GGNCC	3 cut(s)	973, 1111, 1180
Cfr42I	CCGCGG	1 cut(s)	22
Cfr9I	CCCGGG	1 cut(s)	969
CseI	GACGC	6 cut(s)	458, 485, 535, 881, 914, 995
Csp6I	GTAC	6 cut(s)	40, 158, 385, 742, 1327, 1360
CviQI	GTAC	6 cut(s)	40, 158, 385, 742, 1327, 1360
DdeI	CTNAG	4 cut(s)	138, 558, 804, 1092
DpnI	GATC	3 cut(s)	864, 899, 998
DpnII	GATC	3 cut(s)	862, 897, 996
DrdI	GACNNNNNNGTC	1 cut(s)	1109
DseDI	GACNNNNNNGTC	1 cut(s)	1109
EaeI	YGGCCR	2 cut(s)	303, 619
EagI	CGGCCG	1 cut(s)	619
Eam1104I	CTCTTC	2 cut(s)	18, 839
EarI	CTCTTC	2 cut(s)	18, 839
EciI	GGCGGA	2 cut(s)	133, 1409
Ecl136II	GAGCTC	3 cut(s)	30, 1096, 1367
EclXI	CGGCCG	1 cut(s)	619
Eco105I	TACGTA	1 cut(s)	704
Eco130I	CCWWGG	1 cut(s)	1147
Eco24I	GRGCYC	3 cut(s)	32, 1098, 1369
Eco32I	GATATC	2 cut(s)	199, 487
Eco47I	GGWCC	3 cut(s)	973, 1111, 1180
Eco52I	CGGCCG	1 cut(s)	619
Eco53kI	GAGCTC	3 cut(s)	30, 1096, 1367
Eco88I	CYCGRG	5 cut(s)	310, 365, 887, 969, 1037
Eco91I	GGTNACC	2 cut(s)	497, 1019
EcoICRI	GAGCTC	3 cut(s)	30, 1096, 1367
EcoO65I	GGTNACC	2 cut(s)	497, 1019
EcoRII	CCWGG	1 cut(s)	418
EcoRV	GATATC	2 cut(s)	199, 487
EcoT14I	CCWWGG	1 cut(s)	1147
EcoT22I	ATGCAT	1 cut(s)	751
EcoT38I	GRGCYC	3 cut(s)	32, 1098, 1369
ErhI	CCWWGG	1 cut(s)	1147
Esp3I	CGTCTC	2 cut(s)	139, 932
FaqI	GGGAC	5 cut(s)	22, 184, 382, 986, 1097
FauI	CCCGC	2 cut(s)	172, 275
FauNDI	CATATG	1 cut(s)	1031
FbaI	TGATCA	1 cut(s)	996
Fnu4HI	GCNGC	6 cut(s)	70, 224, 711, 1070, 1314, 1407
FokI	GGATG	2 cut(s)	1147, 1189
FriOI	GRGCYC	3 cut(s)	32, 1098, 1369
Fsp4HI	GCNGC	6 cut(s)	70, 224, 711, 1070, 1314, 1407
FspBI	CTAG	5 cut(s)	1010, 1025, 1206, 1245, 1435
GlaI	GCGC	3 cut(s)	217, 352, 453
GluI	GCNGC	6 cut(s)	70, 224, 711, 1070, 1314, 1407
GsuI	CTGGAG	2 cut(s)	783, 846
HaeII	RGCGCY	1 cut(s)	354
HaeIII	GGCC	3 cut(s)	101, 305, 621
HgaI	GACGC	6 cut(s)	458, 485, 535, 881, 914, 995
HhaI	GCGC	3 cut(s)	218, 353, 454
Hin1I	GRCGYC	2 cut(s)	892, 987
Hin6I	GCGC	3 cut(s)	216, 351, 452
HinP1I	GCGC	3 cut(s)	216, 351, 452
HincII	GTYRAC	2 cut(s)	813, 1254
HindII	GTYRAC	2 cut(s)	813, 1254
HindIII	AAGCTT	1 cut(s)	1004
HinfI	GANTC	8 cut(s)	10, 140, 203, 267, 315, 424, 492, 673
HpaI	GTTAAC	1 cut(s)	1254
HphI	GGTGA	5 cut(s)	96, 509, 589, 652, 1031
Hpy166II	GTNNAC	6 cut(s)	158, 742, 813, 1254, 1335, 1344
Hpy188I	TCNGA	8 cut(s)	139, 256, 434, 475, 491, 897, 934, 1134
Hpy188III	TCNNGA	8 cut(s)	169, 367, 482, 523, 1000, 1010, 1198, 1345
Hpy8I	GTNNAC	6 cut(s)	158, 742, 813, 1254, 1335, 1344
Hpy99I	CGWCG	5 cut(s)	280, 452, 479, 620, 989
HpyAV	CCTTC	2 cut(s)	331, 1165
HpyCH4III	ACNGT	2 cut(s)	958, 1390
HpyCH4IV	ACGT	6 cut(s)	154, 383, 546, 703, 1226, 1340
HpyCH4V	TGCA	4 cut(s)	250, 534, 749, 1335
HpyF3I	CTNAG	4 cut(s)	138, 558, 804, 1092
HpySE526I	ACGT	6 cut(s)	154, 383, 546, 703, 1226, 1340
Hsp92I	GRCGYC	2 cut(s)	892, 987
HspAI	GCGC	3 cut(s)	216, 351, 452
Kpn2I	TCCGGA	1 cut(s)	481
KroI	GCCGGC	3 cut(s)	113, 146, 621
KroNI	GCCGGC	3 cut(s)	115, 148, 623
Ksp22I	TGATCA	1 cut(s)	996
KspAI	GTTAAC	1 cut(s)	1254
KspI	CCGCGG	1 cut(s)	22
Kzo9I	GATC	3 cut(s)	862, 897, 996
LmnI	GCTCC	3 cut(s)	27, 484, 1262
Lsp1109I	GCAGC	4 cut(s)	210, 697, 1056, 1393
MaeI	CTAG	5 cut(s)	1010, 1025, 1206, 1245, 1435
MaeII	ACGT	6 cut(s)	154, 383, 546, 703, 1226, 1340
MaeIII	GTNAC	4 cut(s)	497, 907, 1019, 1222
MalI	GATC	3 cut(s)	864, 899, 998
MbiI	CCGCTC	1 cut(s)	884
MboI	GATC	3 cut(s)	862, 897, 996
MboII	GAAGA	4 cut(s)	5, 262, 826, 1186
MhlI	GDGCHC	6 cut(s)	32, 788, 1046, 1098, 1337, 1369
MluCI	AATT	3 cut(s)	230, 590, 1422
MlyI	GAGTC	5 cut(s)	4, 149, 212, 276, 486
MmeI	TCCRAC	2 cut(s)	412, 498
Mph1103I	ATGCAT	1 cut(s)	751
MroI	TCCGGA	1 cut(s)	481
MroNI	GCCGGC	3 cut(s)	113, 146, 621
MroXI	GAANNNNTTC	1 cut(s)	1062
MseI	TTAA	2 cut(s)	456, 1253
MspA1I	CMGCKG	2 cut(s)	21, 1406
MspR9I	CCNGG	6 cut(s)	420, 902, 970, 971, 977, 1375
MvaI	CCWGG	1 cut(s)	420
MvnI	CGCG	8 cut(s)	21, 179, 194, 216, 452, 925, 1160, 1313
NaeI	GCCGGC	3 cut(s)	115, 148, 623
NciI	CCSGG	5 cut(s)	902, 970, 971, 977, 1375
NcoI	CCATGG	1 cut(s)	1147
NdeI	CATATG	1 cut(s)	1031
NdeII	GATC	3 cut(s)	862, 897, 996
NgoMIV	GCCGGC	3 cut(s)	113, 146, 621
NlaIV	GGNNCC	6 cut(s)	472, 787, 974, 1113, 1153, 1182
NmeAIII	GCCGAG	1 cut(s)	1079
NmuCI	GTSAC	3 cut(s)	497, 907, 1019
NsiI	ATGCAT	1 cut(s)	751
NspI	RCATGY	2 cut(s)	1053, 1144
NspV	TTCGAA	2 cut(s)	60, 632
PagI	TCATGA	2 cut(s)	522, 1197
PasI	CCCWGGG	1 cut(s)	419
PcsI	WCGNNNNNNNCGW	3 cut(s)	57, 198, 308
PdiI	GCCGGC	3 cut(s)	115, 148, 623
PdmI	GAANNNNTTC	1 cut(s)	1062
PfeI	GAWTC	3 cut(s)	315, 424, 673
Pfl23II	CGTACG	1 cut(s)	1326
PflFI	GACNNNGTC	1 cut(s)	1025
PflMI	CCANNNNNTGG	2 cut(s)	669, 1285
PkrI	GCNGC	6 cut(s)	71, 225, 712, 1071, 1315, 1408
PleI	GAGTC	5 cut(s)	4, 148, 211, 275, 486
PpsI	GAGTC	5 cut(s)	4, 148, 211, 275, 486
Ppu21I	YACGTR	3 cut(s)	155, 384, 704
Psp124BI	GAGCTC	3 cut(s)	32, 1098, 1369
Psp1406I	AACGTT	2 cut(s)	1226, 1340
Psp6I	CCWGG	1 cut(s)	418
PspEI	GGTNACC	2 cut(s)	497, 1019
PspGI	CCWGG	1 cut(s)	418
PspLI	CGTACG	1 cut(s)	1326
PspN4I	GGNNCC	6 cut(s)	472, 787, 974, 1113, 1153, 1182
PspPI	GGNCC	3 cut(s)	973, 1111, 1180
PsyI	GACNNNGTC	1 cut(s)	1025
PvuII	CAGCTG	1 cut(s)	1406
RsaI	GTAC	6 cut(s)	41, 159, 386, 743, 1328, 1361
RsaNI	GTAC	6 cut(s)	40, 158, 385, 742, 1327, 1360
SacI	GAGCTC	3 cut(s)	32, 1098, 1369
SacII	CCGCGG	1 cut(s)	22
SaqAI	TTAA	2 cut(s)	456, 1253
SatI	GCNGC	6 cut(s)	70, 224, 711, 1070, 1314, 1407
Sau3AI	GATC	3 cut(s)	862, 897, 996
Sau96I	GGNCC	3 cut(s)	973, 1111, 1180
SchI	GAGTC	5 cut(s)	4, 149, 212, 276, 486
ScrFI	CCNGG	6 cut(s)	420, 902, 970, 971, 977, 1375
SduI	GDGCHC	6 cut(s)	32, 788, 1046, 1098, 1337, 1369
SfcI	CTRYAG	1 cut(s)	603
Sfr303I	CCGCGG	1 cut(s)	22
SfuI	TTCGAA	2 cut(s)	60, 632
SgrBI	CCGCGG	1 cut(s)	22
SinI	GGWCC	3 cut(s)	973, 1111, 1180
SmaI	CCCGGG	1 cut(s)	971
SnaBI	TACGTA	1 cut(s)	704
Sse9I	AATT	3 cut(s)	230, 590, 1422
SspMI	CTAG	5 cut(s)	1010, 1025, 1206, 1245, 1435
SstI	GAGCTC	3 cut(s)	32, 1098, 1369
StyD4I	CCNGG	6 cut(s)	418, 900, 968, 969, 975, 1373
StyI	CCWWGG	1 cut(s)	1147
TaaI	ACNGT	2 cut(s)	958, 1390
TaiI	ACGT	6 cut(s)	157, 386, 549, 706, 1229, 1343
TaqI	TCGA	5 cut(s)	60, 201, 632, 861, 1274
TaqII	GACCGA	1 cut(s)	516
TasI	AATT	3 cut(s)	230, 590, 1422
TatI	WGTACW	3 cut(s)	157, 741, 1359
TauI	GCSGC	2 cut(s)	72, 1316
TfiI	GAWTC	3 cut(s)	315, 424, 673
Tru1I	TTAA	2 cut(s)	456, 1253
Tru9I	TTAA	2 cut(s)	456, 1253
TscAI	CASTG	1 cut(s)	247
TseFI	GTSAC	3 cut(s)	497, 907, 1019
TseI	GCWGC	4 cut(s)	223, 710, 1069, 1406
Tsp45I	GTSAC	3 cut(s)	497, 907, 1019
TspDTI	ATGAA	7 cut(s)	200, 511, 708, 826, 1186, 1214, 1233
TspGWI	ACGGA	6 cut(s)	27, 40, 196, 507, 633, 1115
TspMI	CCCGGG	1 cut(s)	969
TspRI	CASTG	1 cut(s)	247
Tth111I	GACNNNGTC	1 cut(s)	1025
Van91I	CCANNNNNTGG	2 cut(s)	669, 1285
VneI	GTGCAC	1 cut(s)	1333
VpaK11BI	GGWCC	3 cut(s)	973, 1111, 1180
XapI	RAATTY	1 cut(s)	230
XbaI	TCTAGA	1 cut(s)	1009
XceI	RCATGY	2 cut(s)	1053, 1144
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1423
XmaI	CCCGGG	1 cut(s)	969
XmnI	GAANNNNTTC	1 cut(s)	1062
XspI	CTAG	5 cut(s)	1010, 1025, 1206, 1245, 1435
Zsp2I	ATGCAT	1 cut(s)	751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.