Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Reverse (-)

3236177 .. 3237724

Gene Structure 1548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1044600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGCCACCACTGTAGAGGAACGTTCTCTTCCTACCTTCTCGACAATCGACCGCTGCCCGTCAATTGGTCGCGAAAAACATACCGTGATGGCGGACATGGATGGCACTTTGCTTAGAGGACGGAGCTCATTCCCTTACTTTGCCCTCGTCGCGTTCGAGGTCGGCGGCATACTAAGGCTTCTCTTTTTACTACTGGCGTCTCCACTCATTGGCCTGCTGTACTACTTTGTATCGGAGTCAGCCGGCATTCGTGTCATGGTTTTTGCCACCTTCGCCGGCATGAGAGTTTCCGACATCGAATCCGTGGCGCGTGCGGTACTCCCCAAGTTCTATTCAGCGGACTTGCACTCCGAGACGTGGAGAGTCTTCTCTTCATGTGGGAAACGCTGCGTTTTGACCGCGAACCCCAGAATCATGGTGGAGCCGTTTTTGAAGGAGTTTTTGGGTGCTGACCTGGTTCTGGGGACTGAGATCGACACCTACAGAGGCAGAGCCACTGGGCGGGTTCTCAATCCCGGAGTTTTGGTCGGTGAAGCCAAGGCCAACGCGCTTAACAAAGCGTTTGGCAACGAGCCCTCGTCCGCACCAGATATCGGGCTCGGTGATCGGAAAACGGATTACCCATTCATGAACCGATGCAAAGAGAGCTACGTGGTTCCTGCTACGCCGGAAGTCGAGGCGGTGAGCCACGACAAGTTGCCAAAACCGATAGTGTTTCACGACGGAAGGCTGGCTCAGAAGCCATCGCCGCTCATGGCTTTGCTTATAATTCTTTGGATCCCGGTCGGTTTTCTTTTAGCTTGCTTGCGAGTAGCAGCCGGCTCACTGCTTCCTATGCACGTCGTATACTACGCTTTTTGGGCGTTGGGCGTACGGGTGTATATCAAAGGGACCCCACCACCTCCCGCCAAGAAATCCACAGGCCAAACCGGAGTTCTTTTCATTTGCTCCCACCGCACTCTCCTCGACCCGATTTTTCTCTCGACCGCTCTAGGCCGGCCCATTCCGGCCGTGACCTACTCTCTCTCGCGCCTGTCGGAGCTCATCTCGCCAATTAAGACCGTCCGGCTCACCCGGGACCGGGTCAAGGACGCGAACCAGATTAAAGAGCTCTTGGAACAAGGCGACCTCGTCATATGCCCCGAGGGAACAACGTGCCGGGAACCATTTTTGCTCCGGTTTTCAGCCCTGTTCGCTGAGCTGACAGACGAGCTCGTCCCCGTAGCCATGTCAAACCGGATGAGCATGTTCCACGGGACGACGGCTCGAGGATGGAAGGGGATGGACCCGTTCTACTTCTTCATGAACCCTAGCCCGGCGTACGAAGTGACGTTTTTGAACAAGCTGCCTTACGAGATGACTTGTGGTGCCGGGAAGTCGAGTCATGACGTGGCGAATTACATACAGAGGAACATCGCGGCGAGCTTGTCGTACGAGTGCACCACCTTCACGCGGAAGGACAAGTACCGGGCTTTGGCGGGGAACGACGGCATCGTGGCGGAAAAGTCTAAGCGCGCGGCTACAGCTAAGAAAATTATGGGCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

516

Amino Acids

56.74

Weight (kDa)

9.16

Isoelectric Point (pI)

39.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	28 - 219	3.1e-63	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	29 - 210	5.5e-25	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	309 - 410	4.1e-07	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	767
AasI	GACNNNNNNGTC	1 cut(s)	1214
AccB1I	GGYRCC	1 cut(s)	1367
AccB7I	CCANNNNNTGG	1 cut(s)	209
AccBSI	CCGCTC	2 cut(s)	751, 989
AccI	GTMKAC	1 cut(s)	846
AclI	AACGTT	1 cut(s)	22
AclWI	GGATC	2 cut(s)	772, 785
AcoI	YGGCCR	2 cut(s)	3, 1008
AcyI	GRCGYC	1 cut(s)	197
AfaI	GTAC	6 cut(s)	221, 318, 873, 1322, 1433, 1466
AgsI	TTSAA	2 cut(s)	433, 1339
AjiI	CACGTC	3 cut(s)	357, 841, 1390
AjnI	CCWGG	1 cut(s)	453
AjuI	GAANNNNNNNTTGG	4 cut(s)	425, 457, 918, 950
Alw21I	GWGCWC	5 cut(s)	128, 1044, 1113, 1215, 1442
Alw26I	GTCTC	2 cut(s)	204, 347
Alw44I	GTGCAC	1 cut(s)	1438
AlwI	GGATC	2 cut(s)	772, 785
Ama87I	CYCGRG	3 cut(s)	1074, 1142, 1266
AoxI	GGCC	7 cut(s)	3, 211, 540, 922, 994, 998, 1008
ApaLI	GTGCAC	1 cut(s)	1438
ApeKI	GCWGC	5 cut(s)	54, 387, 815, 1345, 1542
AspLEI	GCGC	5 cut(s)	310, 550, 1032, 1515, 1517
AspS9I	GGNCC	4 cut(s)	891, 999, 1078, 1285
AsuC2I	CCSGG	9 cut(s)	516, 782, 1075, 1076, 1082, 1160, 1316, 1372, 1469
AsuHPI	GGTGA	4 cut(s)	542, 614, 694, 1063
AvaI	CYCGRG	3 cut(s)	1074, 1142, 1266
AvaII	GGWCC	3 cut(s)	891, 1078, 1285
BaeGI	GKGCMC	1 cut(s)	1442
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	777
BanI	GGYRCC	1 cut(s)	1367
BanII	GRGCYC	6 cut(s)	128, 576, 600, 1044, 1113, 1215
BarI	GAAGNNNNNNTAC	2 cut(s)	1448, 1480
BbsI	GAAGAC	1 cut(s)	358
Bbv12I	GWGCWC	5 cut(s)	128, 1044, 1113, 1215, 1442
BbvI	GCAGC	5 cut(s)	41, 374, 827, 1332, 1529
BccI	CCATC	5 cut(s)	82, 95, 751, 1266, 1276
BceAI	ACGGC	4 cut(s)	409, 995, 1278, 1504
BcgI	CGANNNNNNTGC	2 cut(s)	946, 980
BciT130I	CCWGG	1 cut(s)	455
BcnI	CCSGG	9 cut(s)	516, 782, 1075, 1076, 1082, 1160, 1316, 1372, 1469
BcoDI	GTCTC	2 cut(s)	204, 347
BfaI	CTAG	3 cut(s)	992, 1311, 1546
BfmI	CTRYAG	3 cut(s)	12, 481, 1521
BisI	GCNGC	9 cut(s)	55, 166, 388, 749, 816, 1346, 1419, 1518, 1543
BlpI	GCTNAGC	1 cut(s)	1197
BlsI	GCNGC	9 cut(s)	56, 167, 389, 750, 817, 1347, 1420, 1519, 1544
Bme18I	GGWCC	3 cut(s)	891, 1078, 1285
BmeT110I	CYCGRG	3 cut(s)	1074, 1142, 1266
BmgBI	CACGTC	3 cut(s)	357, 841, 1390
BmgT120I	GGNCC	4 cut(s)	891, 999, 1078, 1285
BmiI	GGNNCC	9 cut(s)	423, 657, 779, 892, 893, 1079, 1164, 1287, 1369
BmrI	ACTGGG	1 cut(s)	507
BmsI	GCATC	2 cut(s)	626, 1500
BmuI	ACTGGG	1 cut(s)	507
BpiI	GAAGAC	1 cut(s)	358
BplI	GAGNNNNNCTC	2 cut(s)	1031, 1063
Bpu1102I	GCTNAGC	1 cut(s)	1197
BpuMI	CCSGG	9 cut(s)	516, 782, 1075, 1076, 1082, 1160, 1316, 1372, 1469
BsaAI	YACGTR	1 cut(s)	652
BsaHI	GRCGYC	1 cut(s)	197
BsaJI	CCNNGG	5 cut(s)	303, 537, 1074, 1143, 1252
BsaWI	WCCGGW	3 cut(s)	929, 1176, 1236
BsaXI	ACNNNNNCTCC	4 cut(s)	332, 362, 510, 540
Bse118I	RCCGGY	4 cut(s)	242, 275, 818, 996
Bse1I	ACTGG	2 cut(s)	198, 502
BseBI	CCWGG	1 cut(s)	455
BseDI	CCNNGG	5 cut(s)	303, 537, 1074, 1143, 1252
BseGI	GGATG	4 cut(s)	106, 1245, 1277, 1287
BseMII	CTCAG	3 cut(s)	459, 749, 1188
BseNI	ACTGG	2 cut(s)	198, 502
BsePI	GCGCGC	1 cut(s)	1513
BseRI	GAGGAG	1 cut(s)	953
BseSI	GKGCMC	1 cut(s)	1442
BseX3I	CGGCCG	1 cut(s)	1008
BseXI	GCAGC	5 cut(s)	41, 374, 827, 1332, 1529
Bsh1285I	CGRYCG	4 cut(s)	52, 786, 987, 1011
BshFI	GGCC	7 cut(s)	5, 213, 542, 924, 996, 1000, 1010
BshNI	GGYRCC	1 cut(s)	1367
BsiEI	CGRYCG	4 cut(s)	52, 786, 987, 1011
BsiHKAI	GWGCWC	5 cut(s)	128, 1044, 1113, 1215, 1442
BsiHKCI	CYCGRG	3 cut(s)	1074, 1142, 1266
BsiWI	CGTACG	3 cut(s)	871, 1320, 1431
BslFI	GGGAC	5 cut(s)	478, 904, 1091, 1202, 1270
BsmAI	GTCTC	2 cut(s)	204, 347
BsmBI	CGTCTC	2 cut(s)	204, 347
BsmFI	GGGAC	5 cut(s)	478, 904, 1091, 1202, 1270
BsmI	GAATGC	1 cut(s)	246
BsnI	GGCC	7 cut(s)	5, 213, 542, 924, 996, 1000, 1010
BsoBI	CYCGRG	3 cut(s)	1074, 1142, 1266
Bsp1286I	GDGCHC	7 cut(s)	128, 576, 600, 1044, 1113, 1215, 1442
Bsp143I	GATC	3 cut(s)	471, 604, 777
Bsp1720I	GCTNAGC	1 cut(s)	1197
Bsp68I	TCGCGA	1 cut(s)	72
BspANI	GGCC	7 cut(s)	5, 213, 542, 924, 996, 1000, 1010
BspCNI	CTCAG	3 cut(s)	460, 748, 1189
BspHI	TCATGA	3 cut(s)	627, 1302, 1384
BspLI	GGNNCC	9 cut(s)	423, 657, 779, 892, 893, 1079, 1164, 1287, 1369
BspPI	GGATC	2 cut(s)	772, 785
BspT107I	GGYRCC	1 cut(s)	1367
BsrBI	CCGCTC	2 cut(s)	751, 989
BsrFI	RCCGGY	4 cut(s)	242, 275, 818, 996
BsrI	ACTGG	2 cut(s)	198, 502
BssAI	RCCGGY	4 cut(s)	242, 275, 818, 996
BssECI	CCNNGG	5 cut(s)	303, 537, 1074, 1143, 1252
BssHII	GCGCGC	1 cut(s)	1513
BssMI	GATC	3 cut(s)	471, 604, 777
BssNAI	GTATAC	1 cut(s)	847
BssNI	GRCGYC	1 cut(s)	197
BssT1I	CCWWGG	1 cut(s)	537
Bst1107I	GTATAC	1 cut(s)	847
Bst2UI	CCWGG	1 cut(s)	455
Bst4CI	ACNGT	3 cut(s)	13, 85, 1063
Bst6I	CTCTTC	2 cut(s)	33, 376
BstACI	GRCGYC	1 cut(s)	197
BstBAI	YACGTR	1 cut(s)	652
BstDEI	CTNAG	7 cut(s)	113, 173, 468, 735, 1197, 1509, 1527
BstDSI	CCRYGG	2 cut(s)	303, 1252
BstF5I	GGATG	4 cut(s)	106, 1245, 1277, 1287
BstHHI	GCGC	5 cut(s)	310, 550, 1032, 1515, 1517
BstKTI	GATC	3 cut(s)	474, 607, 780
BstMAI	GTCTC	2 cut(s)	204, 347
BstMBI	GATC	3 cut(s)	471, 604, 777
BstMCI	CGRYCG	4 cut(s)	52, 786, 987, 1011
BstNI	CCWGG	1 cut(s)	455
BstNSI	RCATGY	1 cut(s)	1249
BstSFI	CTRYAG	3 cut(s)	12, 481, 1521
BstSLI	GKGCMC	1 cut(s)	1442
BstV1I	GCAGC	5 cut(s)	41, 374, 827, 1332, 1529
BstV2I	GAAGAC	1 cut(s)	358
BstX2I	RGATCY	1 cut(s)	777
BstXI	CCANNNNNNTGG	1 cut(s)	415
BstYI	RGATCY	1 cut(s)	777
BstZ17I	GTATAC	1 cut(s)	847
BstZI	CGGCCG	1 cut(s)	1008
BsuRI	GGCC	7 cut(s)	5, 213, 542, 924, 996, 1000, 1010
BtgI	CCRYGG	2 cut(s)	303, 1252
BtgZI	GCGATG	2 cut(s)	729, 1399
BtrI	CACGTC	3 cut(s)	357, 841, 1390
BtsCI	GGATG	4 cut(s)	106, 1245, 1277, 1287
BtsI	GCAGTG	1 cut(s)	824
BtsIMutI	CAGTG	3 cut(s)	9, 495, 824
BtuMI	TCGCGA	1 cut(s)	72
CciI	TCATGA	3 cut(s)	627, 1302, 1384
CfoI	GCGC	5 cut(s)	310, 550, 1032, 1515, 1517
Cfr10I	RCCGGY	4 cut(s)	242, 275, 818, 996
Cfr13I	GGNCC	4 cut(s)	891, 999, 1078, 1285
Cfr9I	CCCGGG	1 cut(s)	1074
CseI	GACGC	2 cut(s)	186, 1100
CsiI	ACCWGGT	1 cut(s)	453
Csp6I	GTAC	6 cut(s)	220, 317, 872, 1321, 1432, 1465
CviQI	GTAC	6 cut(s)	220, 317, 872, 1321, 1432, 1465
DdeI	CTNAG	7 cut(s)	113, 173, 468, 735, 1197, 1509, 1527
DpnI	GATC	3 cut(s)	473, 606, 779
DpnII	GATC	3 cut(s)	471, 604, 777
DrdI	GACNNNNNNGTC	1 cut(s)	1214
DseDI	GACNNNNNNGTC	1 cut(s)	1214
EaeI	YGGCCR	2 cut(s)	3, 1008
EagI	CGGCCG	1 cut(s)	1008
Eam1104I	CTCTTC	2 cut(s)	33, 376
EarI	CTCTTC	2 cut(s)	33, 376
EciI	GGCGGA	2 cut(s)	107, 1514
Ecl136II	GAGCTC	4 cut(s)	126, 1042, 1111, 1213
EclXI	CGGCCG	1 cut(s)	1008
Eco130I	CCWWGG	1 cut(s)	537
Eco24I	GRGCYC	6 cut(s)	128, 576, 600, 1044, 1113, 1215
Eco32I	GATATC	1 cut(s)	592
Eco47I	GGWCC	3 cut(s)	891, 1078, 1285
Eco52I	CGGCCG	1 cut(s)	1008
Eco53kI	GAGCTC	4 cut(s)	126, 1042, 1111, 1213
Eco88I	CYCGRG	3 cut(s)	1074, 1142, 1266
EcoICRI	GAGCTC	4 cut(s)	126, 1042, 1111, 1213
EcoO109I	RGGNCCY	1 cut(s)	891
EcoRII	CCWGG	1 cut(s)	453
EcoRV	GATATC	1 cut(s)	592
EcoT14I	CCWWGG	1 cut(s)	537
EcoT38I	GRGCYC	6 cut(s)	128, 576, 600, 1044, 1113, 1215
ErhI	CCWWGG	1 cut(s)	537
Esp3I	CGTCTC	2 cut(s)	204, 347
FaqI	GGGAC	5 cut(s)	478, 904, 1091, 1202, 1270
FauI	CCCGC	3 cut(s)	495, 913, 1471
FauNDI	CATATG	1 cut(s)	1136
FblI	GTMKAC	1 cut(s)	846
Fnu4HI	GCNGC	9 cut(s)	55, 166, 388, 749, 816, 1346, 1419, 1518, 1543
FokI	GGATG	4 cut(s)	113, 1252, 1284, 1294
FriOI	GRGCYC	6 cut(s)	128, 576, 600, 1044, 1113, 1215
FseI	GGCCGGCC	1 cut(s)	1000
Fsp4HI	GCNGC	9 cut(s)	55, 166, 388, 749, 816, 1346, 1419, 1518, 1543
FspBI	CTAG	3 cut(s)	992, 1311, 1546
GlaI	GCGC	5 cut(s)	309, 549, 1031, 1514, 1516
GluI	GCNGC	9 cut(s)	55, 166, 388, 749, 816, 1346, 1419, 1518, 1543
HaeIII	GGCC	7 cut(s)	5, 213, 542, 924, 996, 1000, 1010
HgaI	GACGC	2 cut(s)	186, 1100
HhaI	GCGC	5 cut(s)	310, 550, 1032, 1515, 1517
Hin1I	GRCGYC	1 cut(s)	197
Hin6I	GCGC	5 cut(s)	308, 548, 1030, 1513, 1515
HinP1I	GCGC	5 cut(s)	308, 548, 1030, 1513, 1515
HinfI	GANTC	5 cut(s)	236, 299, 363, 411, 1381
HphI	GGTGA	4 cut(s)	542, 614, 694, 1063
Hpy166II	GTNNAC	2 cut(s)	847, 1440
Hpy188I	TCNGA	6 cut(s)	235, 292, 352, 609, 738, 1039
Hpy188III	TCNNGA	7 cut(s)	40, 71, 628, 719, 982, 1303, 1385
Hpy8I	GTNNAC	2 cut(s)	847, 1440
Hpy99I	CGWCG	5 cut(s)	152, 725, 845, 1264, 1490
HpyAV	CCTTC	7 cut(s)	46, 280, 427, 720, 1270, 1450, 1456
HpyCH4III	ACNGT	3 cut(s)	13, 85, 1063
HpyCH4IV	ACGT	7 cut(s)	22, 356, 651, 840, 1154, 1331, 1389
HpyCH4V	TGCA	4 cut(s)	346, 639, 838, 1440
HpyF3I	CTNAG	7 cut(s)	113, 173, 468, 735, 1197, 1509, 1527
HpySE526I	ACGT	7 cut(s)	22, 356, 651, 840, 1154, 1331, 1389
Hsp92I	GRCGYC	1 cut(s)	197
HspAI	GCGC	5 cut(s)	308, 548, 1030, 1513, 1515
KflI	GGGWCCC	1 cut(s)	891
KroI	GCCGGC	4 cut(s)	242, 275, 818, 996
KroNI	GCCGGC	4 cut(s)	244, 277, 820, 998
Kzo9I	GATC	3 cut(s)	471, 604, 777
LmnI	GCTCC	5 cut(s)	123, 421, 953, 1039, 1179
Lsp1109I	GCAGC	5 cut(s)	41, 374, 827, 1332, 1529
LweI	GCATC	2 cut(s)	626, 1500
MabI	ACCWGGT	1 cut(s)	453
MaeI	CTAG	3 cut(s)	992, 1311, 1546
MaeII	ACGT	7 cut(s)	22, 356, 651, 840, 1154, 1331, 1389
MaeIII	GTNAC	2 cut(s)	1012, 1327
MalI	GATC	3 cut(s)	473, 606, 779
MbiI	CCGCTC	2 cut(s)	751, 989
MboI	GATC	3 cut(s)	471, 604, 777
MboII	GAAGA	4 cut(s)	20, 358, 363, 1291
MfeI	CAATTG	1 cut(s)	63
MflI	RGATCY	1 cut(s)	777
MhlI	GDGCHC	7 cut(s)	128, 576, 600, 1044, 1113, 1215, 1442
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	63, 768, 1053, 1396, 1533
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	245, 372, 1390
MmeI	TCCRAC	2 cut(s)	315, 1017
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	4 cut(s)	242, 275, 818, 996
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	552, 1056, 1104
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	54, 338
MunI	CAATTG	1 cut(s)	63
Mva1269I	GAATGC	1 cut(s)	246
MvaI	CCWGG	1 cut(s)	455
NaeI	GCCGGC	4 cut(s)	244, 277, 820, 998
NciI	CCSGG	9 cut(s)	516, 782, 1075, 1076, 1082, 1160, 1316, 1372, 1469
NdeI	CATATG	1 cut(s)	1136
NdeII	GATC	3 cut(s)	471, 604, 777
NgoMIV	GCCGGC	4 cut(s)	242, 275, 818, 996
NlaIV	GGNNCC	9 cut(s)	423, 657, 779, 892, 893, 1079, 1164, 1287, 1369
NmuCI	GTSAC	2 cut(s)	1012, 1327
NruI	TCGCGA	1 cut(s)	72
NspI	RCATGY	1 cut(s)	1249
PaeR7I	CTCGAG	1 cut(s)	1266
PagI	TCATGA	3 cut(s)	627, 1302, 1384
PauI	GCGCGC	1 cut(s)	1513
PcsI	WCGNNNNNNNCGW	2 cut(s)	153, 1491
PctI	GAATGC	1 cut(s)	246
PdiI	GCCGGC	4 cut(s)	244, 277, 820, 998
PfeI	GAWTC	2 cut(s)	299, 411
Pfl23II	CGTACG	3 cut(s)	871, 1320, 1431
PflFI	GACNNNGTC	2 cut(s)	1082, 1130
PflMI	CCANNNNNTGG	1 cut(s)	209
PfoI	TCCNGGA	1 cut(s)	514
PkrI	GCNGC	9 cut(s)	56, 167, 389, 750, 817, 1347, 1420, 1519, 1544
PleI	GAGTC	3 cut(s)	244, 371, 1389
PpsI	GAGTC	3 cut(s)	244, 371, 1389
Ppu21I	YACGTR	1 cut(s)	652
PpuMI	RGGWCCY	1 cut(s)	891
PsiI	TTATAA	1 cut(s)	767
Psp124BI	GAGCTC	4 cut(s)	128, 1044, 1113, 1215
Psp1406I	AACGTT	1 cut(s)	22
Psp5II	RGGWCCY	1 cut(s)	891
Psp6I	CCWGG	1 cut(s)	453
PspGI	CCWGG	1 cut(s)	453
PspLI	CGTACG	3 cut(s)	871, 1320, 1431
PspN4I	GGNNCC	9 cut(s)	423, 657, 779, 892, 893, 1079, 1164, 1287, 1369
PspPI	GGNCC	4 cut(s)	891, 999, 1078, 1285
PspPPI	RGGWCCY	1 cut(s)	891
PspXI	VCTCGAGB	1 cut(s)	1266
PsuI	RGATCY	1 cut(s)	777
PsyI	GACNNNGTC	2 cut(s)	1082, 1130
PteI	GCGCGC	1 cut(s)	1513
RigI	GGCCGGCC	1 cut(s)	1000
RruI	TCGCGA	1 cut(s)	72
RsaI	GTAC	6 cut(s)	221, 318, 873, 1322, 1433, 1466
RsaNI	GTAC	6 cut(s)	220, 317, 872, 1321, 1432, 1465
SacI	GAGCTC	4 cut(s)	128, 1044, 1113, 1215
SaqAI	TTAA	3 cut(s)	552, 1056, 1104
SatI	GCNGC	9 cut(s)	55, 166, 388, 749, 816, 1346, 1419, 1518, 1543
Sau3AI	GATC	3 cut(s)	471, 604, 777
Sau96I	GGNCC	4 cut(s)	891, 999, 1078, 1285
SchI	GAGTC	3 cut(s)	245, 372, 1390
SduI	GDGCHC	7 cut(s)	128, 576, 600, 1044, 1113, 1215, 1442
SexAI	ACCWGGT	1 cut(s)	453
SfaNI	GCATC	2 cut(s)	626, 1500
SfcI	CTRYAG	3 cut(s)	12, 481, 1521
Sfr274I	CTCGAG	1 cut(s)	1266
SinI	GGWCC	3 cut(s)	891, 1078, 1285
SlaI	CTCGAG	1 cut(s)	1266
SmaI	CCCGGG	1 cut(s)	1076
SmlI	CTYRAG	1 cut(s)	1266
SmoI	CTYRAG	1 cut(s)	1266
Sse9I	AATT	5 cut(s)	63, 768, 1053, 1396, 1533
SspMI	CTAG	3 cut(s)	992, 1311, 1546
SstI	GAGCTC	4 cut(s)	128, 1044, 1113, 1215
StyI	CCWWGG	1 cut(s)	537
TaaI	ACNGT	3 cut(s)	13, 85, 1063
TaiI	ACGT	7 cut(s)	25, 359, 654, 843, 1157, 1334, 1392
TasI	AATT	5 cut(s)	63, 768, 1053, 1396, 1533
TatI	WGTACW	1 cut(s)	219
TauI	GCSGC	4 cut(s)	168, 751, 1421, 1520
TfiI	GAWTC	2 cut(s)	299, 411
Tru1I	TTAA	3 cut(s)	552, 1056, 1104
Tru9I	TTAA	3 cut(s)	552, 1056, 1104
TscAI	CASTG	3 cut(s)	16, 502, 831
TseFI	GTSAC	2 cut(s)	1012, 1327
TseI	GCWGC	5 cut(s)	54, 387, 815, 1345, 1542
Tsp45I	GTSAC	2 cut(s)	1012, 1327
TspDTI	ATGAA	6 cut(s)	363, 616, 644, 931, 1291, 1319
TspGWI	ACGGA	4 cut(s)	136, 292, 629, 738
TspMI	CCCGGG	1 cut(s)	1074
TspRI	CASTG	3 cut(s)	16, 502, 831
Tth111I	GACNNNGTC	2 cut(s)	1082, 1130
Van91I	CCANNNNNTGG	1 cut(s)	209
VneI	GTGCAC	1 cut(s)	1438
VpaK11BI	GGWCC	3 cut(s)	891, 1078, 1285
XceI	RCATGY	1 cut(s)	1249
XcmI	CCANNNNNNNNNTGG	1 cut(s)	415
XhoI	CTCGAG	1 cut(s)	1266
XmaI	CCCGGG	1 cut(s)	1074
XmiI	GTMKAC	1 cut(s)	846
XspI	CTAG	3 cut(s)	992, 1311, 1546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.