Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Reverse (-)

62861149 .. 62863170

Gene Structure 2022 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G036040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGCTATAACCACCTTCCCAACCATCGACAAATGCTCATCGAACGGCCGAGAAAACCACACGGTGGTTGCGGACATGGATGGGACCTTGCTAAGAGGCCGCAGCTCTTTTCCTTACTTTGCTCTCATTGCTTTTGAGGTCGGTGGGATTTTAAGGCTCCTATTCTTGCTCTTGGTCTCCCCATTAGCTGGAATCCTCTATTACTTCGTATCTGAATCGGCCGGAATCCAAGTTCTGATCTTCGCAACATTTGCCGGTGTGAGGGTGTCTGAGATCGAGTCAGTGGCCCGTGCCGTGCTACCTAAGTTTTACTCGAGTGACATTCACCCTGAGTCGTGGCGCGTGCTCTCTGCGTGCGAGAAGCGGTATATTCTTACTGCCAACCCTAGGATTATGGTGGAAGCATTTTGTAAGGATTTCCTTGGATTTGATTCGGTTTTGGGGACTGAAATAGCAACCTACAAGGGTAAAGCCACTGGCTTTGTTTCTCCACCTGGTGTGCTTGTAGCAGAGAAGAAGGCTGATGCTCTTAAGAAGGCTTTTGGAGATGTACAGCCAGAGATTGGGCTTGGAGATAGACACACTGATATTCCTTTCATGGCACTTTGCAAGGAGGGTTACATGGTTCCGCCCAACCCCCAAGTGGAAGCAGTAACCAGAGAAAAGCTCCCTAAGCCCATAGTCTTCCACGACGGCCGCCTAGTCCAAAAGCCTACACCTCTCATGGCCCTCCTTACCATCCTCTGGCTCCCCATCGGCTTCTTCCTCGCCTGCCTCCGAATCGCCGCCGGCTCCCTCCTACCCATGCCAGTCGTCTATTATGCCTTCTGGGTCCTCGGTGTCCGTGTCACTGTTAAGGGCACCCCACCCCCTCCGGCCAAAAAATCCACCGGCCAGTCCGGCGTCCTCTTCATCTGCTCCCACCGCACCCTCCTCGACCCCATCTTCCTCTCCACCGCCCTCGGCCGCCCCATCCCCGCCGTCACCTACTCCGTCTCTCGTCTGTCGGAGTTCATCTCCCCCATCAAGACCGTTCGCCTCAACCGCGACCGGGCCAAAGACGCCGCCATGATCAAGAAGCTACTAGAAGAAGGTGACCTTGCAATCTGCCCCGAGGGGACCACCTGCCGGGAGCCGTTTCTGTTGAGGTTCTCCGCGCTTTTTGCTGAGCTAACCGACGAGCTCGTTCCGGTGGCGATGGTGAATAAGATGAGCATGTTCCACGGGACGACGGCGCGTGGGTGGAAGGGAATGGACCCTTTTTACTTCTTCATGAACCCTAGCCCAGAATACGAGGTCACATTTTTGAACAAGCTGCCAGCAGAGTTGACTTGCGGGGCGGGCAAGTCGAGCCATGAGGTGGCGAATTATATACAGAGGGCTATAGCGGCAAGCCTTTCGTATGATATCACTAGTTTTACGAGGAAAGACAAATATAGGGCTCTGGCTGGGAACGATGGCTCTGTGGTTGAGAAACCTGTGCTGCAACCCAACAAAGTCATGGGGTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

503

Amino Acids

55.23

Weight (kDa)

8.93

Isoelectric Point (pI)

43.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	21 - 209	2e-60	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	22 - 200	5.4e-24	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	299 - 399	2.7e-07	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1133
Acc36I	ACCTGC	1 cut(s)	1133
AccB1I	GGYRCC	1 cut(s)	860
AccB7I	CCANNNNNTGG	5 cut(s)	64, 188, 563, 744, 1360
AccII	CGCG	4 cut(s)	342, 1047, 1157, 1237
AcoI	YGGCCR	6 cut(s)	46, 219, 694, 876, 892, 964
AcyI	GRCGYC	2 cut(s)	903, 1062
AdeI	CACNNNGTG	2 cut(s)	64, 497
AfaI	GTAC	1 cut(s)	552
AflII	CTTAAG	1 cut(s)	530
AgsI	TTSAA	1 cut(s)	1309
AhlI	ACTAGT	1 cut(s)	1412
AjnI	CCWGG	1 cut(s)	493
AloI	GAACNNNNNNTCC	2 cut(s)	216, 248
AluBI	AGCT	7 cut(s)	105, 188, 667, 1081, 1171, 1183, 1315
AluI	AGCT	7 cut(s)	105, 188, 667, 1081, 1171, 1183, 1315
Alw21I	GWGCWC	2 cut(s)	348, 1185
Alw26I	GTCTC	2 cut(s)	181, 1000
Ama87I	CYCGRG	2 cut(s)	313, 1112
ApeKI	GCWGC	3 cut(s)	102, 1315, 1483
AspA2I	CCTAGG	1 cut(s)	386
AspLEI	GCGC	3 cut(s)	342, 1159, 1237
AspS9I	GGNCC	7 cut(s)	84, 286, 727, 832, 1053, 1119, 1255
AsuC2I	CCSGG	2 cut(s)	1052, 1130
AsuHPI	GGTGA	4 cut(s)	317, 976, 1106, 1213
AvaI	CYCGRG	2 cut(s)	313, 1112
AvaII	GGWCC	4 cut(s)	84, 832, 1119, 1255
AvrII	CCTAGG	1 cut(s)	386
BaeGI	GKGCMC	1 cut(s)	863
BanI	GGYRCC	1 cut(s)	860
BanII	GRGCYC	2 cut(s)	1185, 1444
BbsI	GAAGAC	1 cut(s)	676
Bbv12I	GWGCWC	2 cut(s)	348, 1185
BbvI	GCAGC	3 cut(s)	114, 1302, 1470
BccI	CCATC	9 cut(s)	32, 74, 746, 761, 950, 980, 1031, 1192, 1451
BceAI	ACGGC	6 cut(s)	61, 278, 709, 965, 1120, 1248
BcgI	CGANNNNNNTGC	4 cut(s)	916, 950, 1380, 1414
BciT130I	CCWGG	1 cut(s)	495
BclI	TGATCA	1 cut(s)	1071
BcnI	CCSGG	2 cut(s)	1052, 1130
BcoDI	GTCTC	2 cut(s)	181, 1000
BcuI	ACTAGT	1 cut(s)	1412
BfaI	CTAG	5 cut(s)	387, 701, 1085, 1281, 1413
BfmI	CTRYAG	1 cut(s)	1383
BfrI	CTTAAG	1 cut(s)	530
BfuAI	ACCTGC	1 cut(s)	1133
BglI	GCCNNNNNGGC	1 cut(s)	900
BisI	GCNGC	9 cut(s)	100, 103, 697, 786, 967, 1065, 1316, 1389, 1484
BlnI	CCTAGG	1 cut(s)	386
BlpI	GCTNAGC	1 cut(s)	1167
BlsI	GCNGC	9 cut(s)	101, 104, 698, 787, 968, 1066, 1317, 1390, 1485
Bme1390I	CCNGG	3 cut(s)	495, 1052, 1130
Bme18I	GGWCC	4 cut(s)	84, 832, 1119, 1255
BmeT110I	CYCGRG	2 cut(s)	313, 1112
BmgT120I	GGNCC	7 cut(s)	84, 286, 727, 832, 1053, 1119, 1255
BmrFI	CCNGG	3 cut(s)	495, 1052, 1130
BmsI	GCATC	1 cut(s)	514
BpiI	GAAGAC	1 cut(s)	676
BplI	GAGNNNNNCTC	4 cut(s)	651, 683, 1001, 1033
Bpu10I	CCTNAGC	1 cut(s)	672
Bpu1102I	GCTNAGC	1 cut(s)	1167
BpuMI	CCSGG	2 cut(s)	1052, 1130
BsaHI	GRCGYC	2 cut(s)	903, 1062
BsaI	GGTCTC	1 cut(s)	181
BsaJI	CCNNGG	6 cut(s)	386, 421, 835, 961, 1113, 1222
BsaWI	WCCGGW	1 cut(s)	1189
Bse118I	RCCGGY	3 cut(s)	254, 788, 890
Bse1I	ACTGG	3 cut(s)	481, 809, 895
Bse3DI	GCAATG	1 cut(s)	126
BseBI	CCWGG	1 cut(s)	495
BseDI	CCNNGG	6 cut(s)	386, 421, 835, 961, 1113, 1222
BseGI	GGATG	3 cut(s)	85, 738, 972
BseMI	GCAATG	1 cut(s)	126
BseMII	CTCAG	3 cut(s)	261, 321, 1158
BseNI	ACTGG	3 cut(s)	481, 809, 895
BseRI	GAGGAG	1 cut(s)	923
BseSI	GKGCMC	1 cut(s)	863
BseX3I	CGGCCG	4 cut(s)	46, 219, 694, 964
BseXI	GCAGC	3 cut(s)	114, 1302, 1470
BseYI	CCCAGC	1 cut(s)	1448
Bsh1236I	CGCG	4 cut(s)	342, 1047, 1157, 1237
Bsh1285I	CGRYCG	5 cut(s)	49, 222, 697, 967, 1051
BshNI	GGYRCC	1 cut(s)	860
BsiEI	CGRYCG	5 cut(s)	49, 222, 697, 967, 1051
BsiHKAI	GWGCWC	2 cut(s)	348, 1185
BsiHKCI	CYCGRG	2 cut(s)	313, 1112
BsiSI	CCGG	9 cut(s)	222, 255, 789, 875, 891, 900, 1051, 1129, 1190
BslFI	GGGAC	4 cut(s)	97, 457, 1132, 1240
BsmAI	GTCTC	2 cut(s)	181, 1000
BsmBI	CGTCTC	1 cut(s)	1000
BsmFI	GGGAC	4 cut(s)	97, 457, 1132, 1240
Bso31I	GGTCTC	1 cut(s)	181
BsoBI	CYCGRG	2 cut(s)	313, 1112
Bsp1286I	GDGCHC	4 cut(s)	348, 863, 1185, 1444
Bsp1407I	TGTACA	1 cut(s)	550
Bsp143I	GATC	3 cut(s)	237, 273, 1071
Bsp1720I	GCTNAGC	1 cut(s)	1167
BspCNI	CTCAG	3 cut(s)	262, 322, 1159
BspFNI	CGCG	4 cut(s)	342, 1047, 1157, 1237
BspHI	TCATGA	1 cut(s)	1272
BspMI	ACCTGC	1 cut(s)	1133
BspT107I	GGYRCC	1 cut(s)	860
BspTI	CTTAAG	1 cut(s)	530
BspTNI	GGTCTC	1 cut(s)	181
BsrDI	GCAATG	1 cut(s)	126
BsrFI	RCCGGY	3 cut(s)	254, 788, 890
BsrGI	TGTACA	1 cut(s)	550
BsrI	ACTGG	3 cut(s)	481, 809, 895
BssAI	RCCGGY	3 cut(s)	254, 788, 890
BssECI	CCNNGG	6 cut(s)	386, 421, 835, 961, 1113, 1222
BssMI	GATC	3 cut(s)	237, 273, 1071
BssNI	GRCGYC	2 cut(s)	903, 1062
BssT1I	CCWWGG	2 cut(s)	386, 421
Bst2UI	CCWGG	1 cut(s)	495
Bst4CI	ACNGT	3 cut(s)	64, 853, 1033
Bst6I	CTCTTC	1 cut(s)	914
BstACI	GRCGYC	2 cut(s)	903, 1062
BstAFI	CTTAAG	1 cut(s)	530
BstAPI	GCANNNNNTGC	1 cut(s)	251
BstAUI	TGTACA	1 cut(s)	550
BstC8I	GCNNGC	7 cut(s)	344, 355, 772, 790, 1320, 1342, 1393
BstDEI	CTNAG	6 cut(s)	92, 270, 303, 330, 672, 1167
BstDSI	CCRYGG	1 cut(s)	1222
BstEII	GGTNACC	1 cut(s)	1094
BstF5I	GGATG	3 cut(s)	85, 738, 972
BstFNI	CGCG	4 cut(s)	342, 1047, 1157, 1237
BstHHI	GCGC	3 cut(s)	342, 1159, 1237
BstKTI	GATC	3 cut(s)	240, 276, 1074
BstMAI	GTCTC	2 cut(s)	181, 1000
BstMBI	GATC	3 cut(s)	237, 273, 1071
BstMCI	CGRYCG	5 cut(s)	49, 222, 697, 967, 1051
BstNI	CCWGG	1 cut(s)	495
BstNSI	RCATGY	1 cut(s)	1219
BstPI	GGTNACC	1 cut(s)	1094
BstSCI	CCNGG	3 cut(s)	493, 1050, 1128
BstSFI	CTRYAG	1 cut(s)	1383
BstSLI	GKGCMC	1 cut(s)	863
BstUI	CGCG	4 cut(s)	342, 1047, 1157, 1237
BstV1I	GCAGC	3 cut(s)	114, 1302, 1470
BstV2I	GAAGAC	1 cut(s)	676
BstZI	CGGCCG	4 cut(s)	46, 219, 694, 964
BtgI	CCRYGG	1 cut(s)	1222
BtgZI	GCGATG	1 cut(s)	1211
BtsCI	GGATG	3 cut(s)	85, 738, 972
BtsIMutI	CAGTG	4 cut(s)	288, 474, 582, 849
BveI	ACCTGC	1 cut(s)	1133
Cac8I	GCNNGC	7 cut(s)	344, 355, 772, 790, 1320, 1342, 1393
CciI	TCATGA	1 cut(s)	1272
CfoI	GCGC	3 cut(s)	342, 1159, 1237
Cfr10I	RCCGGY	3 cut(s)	254, 788, 890
Cfr13I	GGNCC	7 cut(s)	84, 286, 727, 832, 1053, 1119, 1255
CseI	GACGC	2 cut(s)	892, 1070
CsiI	ACCWGGT	1 cut(s)	493
Csp6I	GTAC	1 cut(s)	551
CspCI	CAANNNNNGTGG	2 cut(s)	463, 498
CviQI	GTAC	1 cut(s)	551
DdeI	CTNAG	6 cut(s)	92, 270, 303, 330, 672, 1167
DpnI	GATC	3 cut(s)	239, 275, 1073
DpnII	GATC	3 cut(s)	237, 273, 1071
DraIII	CACNNNGTG	2 cut(s)	64, 497
EaeI	YGGCCR	6 cut(s)	46, 219, 694, 876, 892, 964
EagI	CGGCCG	4 cut(s)	46, 219, 694, 964
Eam1104I	CTCTTC	1 cut(s)	914
EarI	CTCTTC	1 cut(s)	914
EciI	GGCGGA	1 cut(s)	618
Ecl136II	GAGCTC	1 cut(s)	1183
EclXI	CGGCCG	4 cut(s)	46, 219, 694, 964
Eco130I	CCWWGG	2 cut(s)	386, 421
Eco24I	GRGCYC	2 cut(s)	1185, 1444
Eco31I	GGTCTC	1 cut(s)	181
Eco32I	GATATC	1 cut(s)	1408
Eco47I	GGWCC	4 cut(s)	84, 832, 1119, 1255
Eco52I	CGGCCG	4 cut(s)	46, 219, 694, 964
Eco53kI	GAGCTC	1 cut(s)	1183
Eco88I	CYCGRG	2 cut(s)	313, 1112
Eco91I	GGTNACC	1 cut(s)	1094
EcoICRI	GAGCTC	1 cut(s)	1183
EcoO109I	RGGNCCY	2 cut(s)	84, 832
EcoO65I	GGTNACC	1 cut(s)	1094
EcoRII	CCWGG	1 cut(s)	493
EcoRV	GATATC	1 cut(s)	1408
EcoT14I	CCWWGG	2 cut(s)	386, 421
EcoT38I	GRGCYC	2 cut(s)	1185, 1444
ErhI	CCWWGG	2 cut(s)	386, 421
Esp3I	CGTCTC	1 cut(s)	1000
FalI	AAGNNNNNCTT	2 cut(s)	1083, 1115
FaqI	GGGAC	4 cut(s)	97, 457, 1132, 1240
FauI	CCCGC	3 cut(s)	985, 1328, 1333
FbaI	TGATCA	1 cut(s)	1071
Fnu4HI	GCNGC	9 cut(s)	100, 103, 697, 786, 967, 1065, 1316, 1389, 1484
FokI	GGATG	3 cut(s)	92, 725, 959
FriOI	GRGCYC	2 cut(s)	1185, 1444
Fsp4HI	GCNGC	9 cut(s)	100, 103, 697, 786, 967, 1065, 1316, 1389, 1484
FspBI	CTAG	5 cut(s)	387, 701, 1085, 1281, 1413
GlaI	GCGC	3 cut(s)	341, 1158, 1236
GluI	GCNGC	9 cut(s)	100, 103, 697, 786, 967, 1065, 1316, 1389, 1484
GsaI	CCCAGC	1 cut(s)	1452
HapII	CCGG	9 cut(s)	222, 255, 789, 875, 891, 900, 1051, 1129, 1190
HgaI	GACGC	2 cut(s)	892, 1070
HhaI	GCGC	3 cut(s)	342, 1159, 1237
Hin1I	GRCGYC	2 cut(s)	903, 1062
Hin6I	GCGC	3 cut(s)	340, 1157, 1235
HinP1I	GCGC	3 cut(s)	340, 1157, 1235
HincII	GTYRAC	1 cut(s)	1329
HindII	GTYRAC	1 cut(s)	1329
HinfI	GANTC	7 cut(s)	192, 215, 225, 278, 332, 431, 780
HpaII	CCGG	9 cut(s)	222, 255, 789, 875, 891, 900, 1051, 1129, 1190
HphI	GGTGA	4 cut(s)	317, 976, 1106, 1213
Hpy166II	GTNNAC	1 cut(s)	1329
Hpy188I	TCNGA	5 cut(s)	214, 237, 271, 779, 1009
Hpy188III	TCNNGA	3 cut(s)	1027, 1075, 1273
Hpy8I	GTNNAC	1 cut(s)	1329
Hpy99I	CGWCG	3 cut(s)	695, 1181, 1234
HpyAV	CCTTC	6 cut(s)	25, 511, 529, 835, 1085, 1240
HpyCH4III	ACNGT	3 cut(s)	64, 853, 1033
HpyCH4V	TGCA	3 cut(s)	609, 1103, 1486
HpyF3I	CTNAG	6 cut(s)	92, 270, 303, 330, 672, 1167
Hsp92I	GRCGYC	2 cut(s)	903, 1062
HspAI	GCGC	3 cut(s)	340, 1157, 1235
KroI	GCCGGC	1 cut(s)	788
KroNI	GCCGGC	1 cut(s)	790
Ksp22I	TGATCA	1 cut(s)	1071
Kzo9I	GATC	3 cut(s)	237, 273, 1071
LmnI	GCTCC	6 cut(s)	162, 672, 753, 797, 923, 1132
Lsp1109I	GCAGC	3 cut(s)	114, 1302, 1470
LweI	GCATC	1 cut(s)	514
MabI	ACCWGGT	1 cut(s)	493
MaeI	CTAG	5 cut(s)	387, 701, 1085, 1281, 1413
MaeIII	GTNAC	7 cut(s)	317, 617, 652, 847, 982, 1094, 1297
MalI	GATC	3 cut(s)	239, 275, 1073
MboI	GATC	3 cut(s)	237, 273, 1071
MboII	GAAGA	8 cut(s)	232, 526, 676, 754, 901, 937, 1100, 1261
MhlI	GDGCHC	4 cut(s)	348, 863, 1185, 1444
MluCI	AATT	1 cut(s)	1366
MlyI	GAGTC	2 cut(s)	287, 341
MmeI	TCCRAC	1 cut(s)	987
MroNI	GCCGGC	1 cut(s)	788
MseI	TTAA	3 cut(s)	152, 531, 855
MspCI	CTTAAG	1 cut(s)	530
MspI	CCGG	9 cut(s)	222, 255, 789, 875, 891, 900, 1051, 1129, 1190
MspR9I	CCNGG	3 cut(s)	495, 1052, 1130
MvaI	CCWGG	1 cut(s)	495
MvnI	CGCG	4 cut(s)	342, 1047, 1157, 1237
NaeI	GCCGGC	1 cut(s)	790
NciI	CCSGG	2 cut(s)	1052, 1130
NdeII	GATC	3 cut(s)	237, 273, 1071
NgoMIV	GCCGGC	1 cut(s)	788
NmeAIII	GCCGAG	2 cut(s)	74, 942
NmuCI	GTSAC	5 cut(s)	317, 847, 982, 1094, 1297
NspI	RCATGY	1 cut(s)	1219
PaeR7I	CTCGAG	1 cut(s)	313
PagI	TCATGA	1 cut(s)	1272
PaqCI	CACCTGC	1 cut(s)	1133
PdiI	GCCGGC	1 cut(s)	790
PfeI	GAWTC	5 cut(s)	192, 215, 225, 431, 780
PflMI	CCANNNNNTGG	5 cut(s)	64, 188, 563, 744, 1360
PkrI	GCNGC	9 cut(s)	101, 104, 698, 787, 968, 1066, 1317, 1390, 1485
PleI	GAGTC	2 cut(s)	286, 340
PpsI	GAGTC	2 cut(s)	286, 340
PpuMI	RGGWCCY	2 cut(s)	84, 832
Psp124BI	GAGCTC	1 cut(s)	1185
Psp5II	RGGWCCY	2 cut(s)	84, 832
Psp6I	CCWGG	1 cut(s)	493
PspEI	GGTNACC	1 cut(s)	1094
PspFI	CCCAGC	1 cut(s)	1448
PspGI	CCWGG	1 cut(s)	493
PspPI	GGNCC	7 cut(s)	84, 286, 727, 832, 1053, 1119, 1255
PspPPI	RGGWCCY	2 cut(s)	84, 832
PspXI	VCTCGAGB	1 cut(s)	313
RsaI	GTAC	1 cut(s)	552
RsaNI	GTAC	1 cut(s)	551
SacI	GAGCTC	1 cut(s)	1185
SaqAI	TTAA	3 cut(s)	152, 531, 855
SatI	GCNGC	9 cut(s)	100, 103, 697, 786, 967, 1065, 1316, 1389, 1484
Sau3AI	GATC	3 cut(s)	237, 273, 1071
Sau96I	GGNCC	7 cut(s)	84, 286, 727, 832, 1053, 1119, 1255
SchI	GAGTC	2 cut(s)	287, 341
ScrFI	CCNGG	3 cut(s)	495, 1052, 1130
SduI	GDGCHC	4 cut(s)	348, 863, 1185, 1444
SexAI	ACCWGGT	1 cut(s)	493
SfaNI	GCATC	1 cut(s)	514
SfcI	CTRYAG	1 cut(s)	1383
Sfr274I	CTCGAG	1 cut(s)	313
SinI	GGWCC	4 cut(s)	84, 832, 1119, 1255
SlaI	CTCGAG	1 cut(s)	313
SmlI	CTYRAG	2 cut(s)	313, 530
SmoI	CTYRAG	2 cut(s)	313, 530
SpeI	ACTAGT	1 cut(s)	1412
Sse9I	AATT	1 cut(s)	1366
SspMI	CTAG	5 cut(s)	387, 701, 1085, 1281, 1413
SstI	GAGCTC	1 cut(s)	1185
StyD4I	CCNGG	3 cut(s)	493, 1050, 1128
StyI	CCWWGG	2 cut(s)	386, 421
TaaI	ACNGT	3 cut(s)	64, 853, 1033
TaqI	TCGA	6 cut(s)	27, 41, 276, 314, 936, 1349
TasI	AATT	1 cut(s)	1366
TatI	WGTACW	1 cut(s)	550
TauI	GCSGC	6 cut(s)	102, 699, 788, 969, 1067, 1391
TfiI	GAWTC	5 cut(s)	192, 215, 225, 431, 780
Tru1I	TTAA	3 cut(s)	152, 531, 855
Tru9I	TTAA	3 cut(s)	152, 531, 855
TscAI	CASTG	4 cut(s)	288, 481, 589, 856
TseFI	GTSAC	5 cut(s)	317, 847, 982, 1094, 1297
TseI	GCWGC	3 cut(s)	102, 1315, 1483
Tsp45I	GTSAC	5 cut(s)	317, 847, 982, 1094, 1297
TspDTI	ATGAA	5 cut(s)	586, 901, 1003, 1261, 1289
TspGWI	ACGGA	2 cut(s)	833, 982
TspRI	CASTG	4 cut(s)	288, 481, 589, 856
Van91I	CCANNNNNTGG	5 cut(s)	64, 188, 563, 744, 1360
Vha464I	CTTAAG	1 cut(s)	530
VpaK11BI	GGWCC	4 cut(s)	84, 832, 1119, 1255
XceI	RCATGY	1 cut(s)	1219
XcmI	CCANNNNNNNNNTGG	2 cut(s)	640, 1498
XhoI	CTCGAG	1 cut(s)	313
XmaJI	CCTAGG	1 cut(s)	386
XspI	CTAG	5 cut(s)	387, 701, 1085, 1281, 1413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.