Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Forward (+)

5778375 .. 5780089

Gene Structure 1715 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1082600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGCGAAGTTCCCGACGGTTGATCAGTGCACATCGGATGGACGGGATAAGCACACGGTGGTCGCCGACATGGACGGGACTTTGCTTAGAGGCCGAAGCTCCTTCCCTTACTTTGCTCTTATTGCCTTTGAGGTTGGTGGGATTCTGAGGCTCCTATTCTTGCTTTTAGTTACACCACTAGCCGGACTCCTCTACTACTTTGTCTCGGAATCAGCCGGAATCCAAGTTCTCATCTTTGCAACGTTTGCCGGCGTTAGGGTTTCGGAGATCGAGTCCGTGGCCAGAGCCGTGCTGCCTAAGTTTTACTCGAGCGATTTGCACCCCGAGTCTTGGCGCGTTCTCTCTGCATGCGAAAAGAGATGTATTCTTACTGCAAATCCGAGGATCATGGTGGAGCCTTTCTTGAAGGATTTTCTTGGATTCGACACGGTTTTGGGGACGGAAGTGGCGACTTATAAGGGTAAGGCTACTGGGTTTGTTTCCCCACCTGGTGTGCTTGTGGGAAAGAAGAAGGCAGATGCTCTTAAGAAGGCTTTTGGAGATGTCCAGCCAGAGATTGGGCTTGGTGATAGGCACACTGACATTCCTTTCATGAAATTATGCAAGGAGGGTTACATGGTGCCAGCAATACAAGAAGTGGAAGCAGTAACAAATGACAAGCTACCAAAGCCCATAATCTTCCACGACGGCCGTCTTGTCCAAAAGCCAACACCTCTCGTGGCGCTCCTCACCCTCCTCTGGCTCCCCATCGGCTTCTTCCTCGCCTGCCTCCGTATCTCAGCCGGGGCACTCCTCCCCATGTCGCTCGTCTACTATGCCTTCTGGGCCCTCGGCGTCCGCATCACAGTCAAGGGCACCCCACCTCCCGCCCCCAAGAAACTCACCGGCGTCCTCTTCATCTGCTCCCACCGCACCCTCCTCGATCCCATCTTCCTCTCCACCGCCCTCGGCCGCCCCATTCCCGCCGTCACCTACTCCGTCTCCCGCCTCTCCGAGTTCATCTCCCCAATCAAGACCGTCCGCCTCAACCGCGACCGGGCCCTCGACGCCGCCATGATCAAGAAGCTTCTAGAAGAGGGTGACCTGGCAATCTGTCCGGAAGGCACCACGTGTCGGGAACCCTTCCTCCTGAGATTCTCCGCACTTTTCGCGGAGCTGACGGACGAGCTGGTCCCGGTGGCGATGGTCAACAAAATGAGCATGTTCCACGGGACCACCGCACGTGGGTGGAAAGGGATGGACCCGTTCTACTTCTTCATGAACCCGAGCCCGGCGTACGAGGTGACCTTTTTGAATAAGCTGCCGGCGGAGATGACGTGTGGGGCCGGGAAGTCGAGCCATGAGGTGGCGAATTATATTCAGAGGGTTATAGCGGCCAGCCTTTCGTATGAATACACCGGGTTCACCAGGAAGGATAAGTACCGGGCTCTTGCCGGAAATGATGGGACCGTGACTGAGAAGCCGTTGCTCCAACCCAGTAAAGTCATGGGCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

499

Amino Acids

54.76

Weight (kDa)

8.99

Isoelectric Point (pI)

37.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	19 - 207	1.3e-61	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	20 - 199	2.8e-26	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	295 - 394	2.5e-06	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	456
AasI	GACNNNNNNGTC	1 cut(s)	1169
AccB1I	GGYRCC	3 cut(s)	619, 854, 1103
AccB7I	CCANNNNNTGG	2 cut(s)	557, 1345
AccI	GTMKAC	1 cut(s)	810
AccII	CGCG	3 cut(s)	336, 1032, 1151
AccIII	TCCGGA	1 cut(s)	1096
AclI	AACGTT	1 cut(s)	242
AclWI	GGATC	2 cut(s)	392, 917
AcoI	YGGCCR	4 cut(s)	279, 688, 949, 1374
AcvI	CACGTG	2 cut(s)	1110, 1223
AcyI	GRCGYC	3 cut(s)	834, 888, 1047
AdeI	CACNNNGTG	3 cut(s)	58, 491, 1110
AfaI	GTAC	2 cut(s)	1277, 1421
AflII	CTTAAG	1 cut(s)	524
AflIII	ACRYGT	2 cut(s)	1109, 1316
AgsI	TTSAA	2 cut(s)	406, 1294
AjiI	CACGTC	1 cut(s)	1317
AjnI	CCWGG	3 cut(s)	487, 1083, 1406
AleI	CACNNNNGTG	1 cut(s)	1225
AloI	GAACNNNNNNTCC	4 cut(s)	210, 242, 1110, 1142
AluBI	AGCT	6 cut(s)	99, 661, 1066, 1156, 1168, 1300
AluI	AGCT	6 cut(s)	99, 661, 1066, 1156, 1168, 1300
Alw21I	GWGCWC	1 cut(s)	32
Alw26I	GTCTC	2 cut(s)	208, 985
Alw44I	GTGCAC	1 cut(s)	28
AlwI	GGATC	2 cut(s)	392, 917
Ama87I	CYCGRG	3 cut(s)	307, 323, 1264
Aor13HI	TCCGGA	1 cut(s)	1096
AoxI	GGCC	8 cut(s)	91, 279, 688, 825, 949, 1038, 1323, 1374
ApaI	GGGCCC	2 cut(s)	829, 1042
ApaLI	GTGCAC	1 cut(s)	28
ApeKI	GCWGC	3 cut(s)	292, 1300, 1491
AspLEI	GCGC	2 cut(s)	336, 724
AspS9I	GGNCC	9 cut(s)	825, 826, 1038, 1039, 1171, 1212, 1240, 1323, 1446
AsuC2I	CCSGG	7 cut(s)	784, 1037, 1175, 1271, 1327, 1399, 1424
AsuHPI	GGTGA	7 cut(s)	578, 721, 874, 961, 1091, 1294, 1396
AvaI	CYCGRG	3 cut(s)	307, 323, 1264
AvaII	GGWCC	4 cut(s)	1171, 1212, 1240, 1446
BaeGI	GKGCMC	5 cut(s)	32, 790, 829, 857, 1042
BalI	TGGCCA	1 cut(s)	281
BanI	GGYRCC	3 cut(s)	619, 854, 1103
BanII	GRGCYC	4 cut(s)	829, 1042, 1271, 1429
BarI	GAAGNNNNNNTAC	2 cut(s)	1403, 1435
BauI	CACGAG	1 cut(s)	716
BbrPI	CACGTG	2 cut(s)	1110, 1223
Bbv12I	GWGCWC	1 cut(s)	32
BbvI	GCAGC	3 cut(s)	279, 1287, 1478
BccI	CCATC	6 cut(s)	32, 755, 935, 1177, 1231, 1436
BceAI	ACGGC	5 cut(s)	272, 675, 703, 950, 1447
BcgI	CGANNNNNNTGC	4 cut(s)	298, 332, 901, 935
BciT130I	CCWGG	3 cut(s)	489, 1085, 1408
BclI	TGATCA	2 cut(s)	22, 1056
BcnI	CCSGG	7 cut(s)	784, 1037, 1175, 1271, 1327, 1399, 1424
BcoDI	GTCTC	2 cut(s)	208, 985
BfaI	CTAG	3 cut(s)	179, 1070, 1495
BfoI	RGCGCY	1 cut(s)	725
BfrI	CTTAAG	1 cut(s)	524
BglI	GCCNNNNNGGC	1 cut(s)	824
BisI	GCNGC	6 cut(s)	293, 952, 1050, 1301, 1374, 1492
BlsI	GCNGC	6 cut(s)	294, 953, 1051, 1302, 1375, 1493
Bme18I	GGWCC	4 cut(s)	1171, 1212, 1240, 1446
BmeT110I	CYCGRG	3 cut(s)	307, 323, 1264
BmgBI	CACGTC	1 cut(s)	1317
BmgT120I	GGNCC	9 cut(s)	825, 826, 1038, 1039, 1171, 1212, 1240, 1323, 1446
BmrI	ACTGGG	2 cut(s)	480, 1470
BmsI	GCATC	2 cut(s)	508, 849
BmuI	ACTGGG	2 cut(s)	480, 1470
BoxI	GACNNNNGTC	1 cut(s)	690
BplI	GAGNNNNNCTC	2 cut(s)	986, 1018
BpuMI	CCSGG	7 cut(s)	784, 1037, 1175, 1271, 1327, 1399, 1424
BsaAI	YACGTR	2 cut(s)	1110, 1223
BsaHI	GRCGYC	3 cut(s)	834, 888, 1047
BsaJI	CCNNGG	6 cut(s)	276, 380, 783, 829, 946, 1207
BsaWI	WCCGGW	1 cut(s)	1096
Bse118I	RCCGGY	3 cut(s)	248, 884, 1303
Bse1I	ACTGG	2 cut(s)	475, 1476
BseAI	TCCGGA	1 cut(s)	1096
BseBI	CCWGG	3 cut(s)	489, 1085, 1408
BseDI	CCNNGG	6 cut(s)	276, 380, 783, 829, 946, 1207
BseGI	GGATG	2 cut(s)	43, 1242
BseMII	CTCAG	4 cut(s)	137, 792, 1121, 1446
BseNI	ACTGG	2 cut(s)	475, 1476
BseRI	GAGGAG	5 cut(s)	179, 716, 725, 782, 908
BseSI	GKGCMC	5 cut(s)	32, 790, 829, 857, 1042
BseX3I	CGGCCG	2 cut(s)	688, 949
BseXI	GCAGC	3 cut(s)	279, 1287, 1478
Bsh1236I	CGCG	3 cut(s)	336, 1032, 1151
Bsh1285I	CGRYCG	3 cut(s)	691, 952, 1036
BshFI	GGCC	8 cut(s)	93, 281, 690, 827, 951, 1040, 1325, 1376
BshNI	GGYRCC	3 cut(s)	619, 854, 1103
BsiEI	CGRYCG	3 cut(s)	691, 952, 1036
BsiHKAI	GWGCWC	1 cut(s)	32
BsiHKCI	CYCGRG	3 cut(s)	307, 323, 1264
BsiWI	CGTACG	1 cut(s)	1275
BslFI	GGGAC	5 cut(s)	91, 451, 1157, 1225, 1459
BsmAI	GTCTC	2 cut(s)	208, 985
BsmBI	CGTCTC	1 cut(s)	985
BsmFI	GGGAC	5 cut(s)	91, 451, 1157, 1225, 1459
BsnI	GGCC	8 cut(s)	93, 281, 690, 827, 951, 1040, 1325, 1376
BsoBI	CYCGRG	3 cut(s)	307, 323, 1264
Bsp120I	GGGCCC	2 cut(s)	825, 1038
Bsp1286I	GDGCHC	7 cut(s)	32, 790, 829, 857, 1042, 1271, 1429
Bsp13I	TCCGGA	1 cut(s)	1096
Bsp143I	GATC	5 cut(s)	22, 267, 384, 922, 1056
BspANI	GGCC	8 cut(s)	93, 281, 690, 827, 951, 1040, 1325, 1376
BspCNI	CTCAG	4 cut(s)	138, 791, 1122, 1447
BspEI	TCCGGA	1 cut(s)	1096
BspFNI	CGCG	3 cut(s)	336, 1032, 1151
BspHI	TCATGA	2 cut(s)	591, 1257
BspPI	GGATC	2 cut(s)	392, 917
BspT107I	GGYRCC	3 cut(s)	619, 854, 1103
BspTI	CTTAAG	1 cut(s)	524
BsrFI	RCCGGY	3 cut(s)	248, 884, 1303
BsrI	ACTGG	2 cut(s)	475, 1476
BssAI	RCCGGY	3 cut(s)	248, 884, 1303
BssECI	CCNNGG	6 cut(s)	276, 380, 783, 829, 946, 1207
BssMI	GATC	5 cut(s)	22, 267, 384, 922, 1056
BssNI	GRCGYC	3 cut(s)	834, 888, 1047
BssSI	CACGAG	1 cut(s)	716
Bst2BI	CACGAG	1 cut(s)	716
Bst2UI	CCWGG	3 cut(s)	489, 1085, 1408
Bst4CI	ACNGT	6 cut(s)	19, 58, 430, 847, 1018, 1450
Bst6I	CTCTTC	2 cut(s)	899, 1068
BstACI	GRCGYC	3 cut(s)	834, 888, 1047
BstAFI	CTTAAG	1 cut(s)	524
BstAPI	GCANNNNNTGC	1 cut(s)	245
BstBAI	YACGTR	2 cut(s)	1110, 1223
BstC8I	GCNNGC	6 cut(s)	250, 349, 624, 766, 1305, 1378
BstDEI	CTNAG	6 cut(s)	86, 146, 297, 778, 1130, 1455
BstDSI	CCRYGG	2 cut(s)	276, 1207
BstEII	GGTNACC	2 cut(s)	1079, 1282
BstF5I	GGATG	2 cut(s)	43, 1242
BstFNI	CGCG	3 cut(s)	336, 1032, 1151
BstH2I	RGCGCY	1 cut(s)	725
BstHHI	GCGC	2 cut(s)	336, 724
BstKTI	GATC	5 cut(s)	25, 270, 387, 925, 1059
BstMAI	GTCTC	2 cut(s)	208, 985
BstMBI	GATC	5 cut(s)	22, 267, 384, 922, 1056
BstMCI	CGRYCG	3 cut(s)	691, 952, 1036
BstMWI	GCNNNNNNNGC	8 cut(s)	122, 245, 667, 824, 909, 1029, 1046, 1148
BstNI	CCWGG	3 cut(s)	489, 1085, 1408
BstNSI	RCATGY	2 cut(s)	351, 1204
BstPAI	GACNNNNGTC	1 cut(s)	690
BstPI	GGTNACC	2 cut(s)	1079, 1282
BstSLI	GKGCMC	5 cut(s)	32, 790, 829, 857, 1042
BstUI	CGCG	3 cut(s)	336, 1032, 1151
BstV1I	GCAGC	3 cut(s)	279, 1287, 1478
BstZI	CGGCCG	2 cut(s)	688, 949
BsuRI	GGCC	8 cut(s)	93, 281, 690, 827, 951, 1040, 1325, 1376
BtgI	CCRYGG	2 cut(s)	276, 1207
BtgZI	GCGATG	1 cut(s)	1196
BtrI	CACGTC	1 cut(s)	1317
BtsCI	GGATG	2 cut(s)	43, 1242
BtsIMutI	CAGTG	2 cut(s)	32, 576
Cac8I	GCNNGC	6 cut(s)	250, 349, 624, 766, 1305, 1378
CciI	TCATGA	2 cut(s)	591, 1257
CfoI	GCGC	2 cut(s)	336, 724
Cfr10I	RCCGGY	3 cut(s)	248, 884, 1303
Cfr13I	GGNCC	9 cut(s)	825, 826, 1038, 1039, 1171, 1212, 1240, 1323, 1446
CseI	GACGC	3 cut(s)	823, 877, 1055
CsiI	ACCWGGT	1 cut(s)	487
Csp6I	GTAC	2 cut(s)	1276, 1420
CviQI	GTAC	2 cut(s)	1276, 1420
DdeI	CTNAG	6 cut(s)	86, 146, 297, 778, 1130, 1455
DpnI	GATC	5 cut(s)	24, 269, 386, 924, 1058
DpnII	GATC	5 cut(s)	22, 267, 384, 922, 1056
DraIII	CACNNNGTG	3 cut(s)	58, 491, 1110
DrdI	GACNNNNNNGTC	1 cut(s)	1169
DseDI	GACNNNNNNGTC	1 cut(s)	1169
EaeI	YGGCCR	4 cut(s)	279, 688, 949, 1374
EagI	CGGCCG	2 cut(s)	688, 949
Eam1104I	CTCTTC	2 cut(s)	899, 1068
EarI	CTCTTC	2 cut(s)	899, 1068
EciI	GGCGGA	2 cut(s)	1010, 1322
EclXI	CGGCCG	2 cut(s)	688, 949
Eco24I	GRGCYC	4 cut(s)	829, 1042, 1271, 1429
Eco47I	GGWCC	4 cut(s)	1171, 1212, 1240, 1446
Eco52I	CGGCCG	2 cut(s)	688, 949
Eco72I	CACGTG	2 cut(s)	1110, 1223
Eco88I	CYCGRG	3 cut(s)	307, 323, 1264
Eco91I	GGTNACC	2 cut(s)	1079, 1282
EcoO109I	RGGNCCY	2 cut(s)	826, 1039
EcoO65I	GGTNACC	2 cut(s)	1079, 1282
EcoRII	CCWGG	3 cut(s)	487, 1083, 1406
EcoT38I	GRGCYC	4 cut(s)	829, 1042, 1271, 1429
Esp3I	CGTCTC	1 cut(s)	985
FaqI	GGGAC	5 cut(s)	91, 451, 1157, 1225, 1459
FauI	CCCGC	3 cut(s)	874, 970, 992
FbaI	TGATCA	2 cut(s)	22, 1056
FblI	GTMKAC	1 cut(s)	810
Fnu4HI	GCNGC	6 cut(s)	293, 952, 1050, 1301, 1374, 1492
FokI	GGATG	2 cut(s)	50, 1249
FriOI	GRGCYC	4 cut(s)	829, 1042, 1271, 1429
Fsp4HI	GCNGC	6 cut(s)	293, 952, 1050, 1301, 1374, 1492
FspBI	CTAG	3 cut(s)	179, 1070, 1495
GlaI	GCGC	2 cut(s)	335, 723
GluI	GCNGC	6 cut(s)	293, 952, 1050, 1301, 1374, 1492
HaeII	RGCGCY	1 cut(s)	725
HaeIII	GGCC	8 cut(s)	93, 281, 690, 827, 951, 1040, 1325, 1376
HgaI	GACGC	3 cut(s)	823, 877, 1055
HhaI	GCGC	2 cut(s)	336, 724
Hin1I	GRCGYC	3 cut(s)	834, 888, 1047
Hin6I	GCGC	2 cut(s)	334, 722
HinP1I	GCGC	2 cut(s)	334, 722
HincII	GTYRAC	1 cut(s)	1189
HindII	GTYRAC	1 cut(s)	1189
HindIII	AAGCTT	1 cut(s)	1064
HinfI	GANTC	8 cut(s)	142, 186, 209, 219, 272, 326, 420, 1134
HphI	GGTGA	7 cut(s)	578, 721, 874, 961, 1091, 1294, 1396
Hpy166II	GTNNAC	4 cut(s)	30, 811, 1189, 1404
Hpy188I	TCNGA	7 cut(s)	37, 147, 208, 265, 381, 994, 1362
Hpy8I	GTNNAC	4 cut(s)	30, 811, 1189, 1404
Hpy99I	CGWCG	3 cut(s)	19, 689, 1049
HpyAV	CCTTC	8 cut(s)	112, 400, 505, 523, 829, 1094, 1132, 1405
HpyCH4III	ACNGT	6 cut(s)	19, 58, 430, 847, 1018, 1450
HpyCH4IV	ACGT	4 cut(s)	242, 1109, 1222, 1316
HpyCH4V	TGCA	6 cut(s)	30, 239, 319, 347, 374, 603
HpyF10VI	GCNNNNNNNGC	8 cut(s)	122, 245, 667, 824, 909, 1029, 1046, 1148
HpyF3I	CTNAG	6 cut(s)	86, 146, 297, 778, 1130, 1455
HpySE526I	ACGT	4 cut(s)	242, 1109, 1222, 1316
Hsp92I	GRCGYC	3 cut(s)	834, 888, 1047
HspAI	GCGC	2 cut(s)	334, 722
Kpn2I	TCCGGA	1 cut(s)	1096
KroI	GCCGGC	2 cut(s)	248, 1303
KroNI	GCCGGC	2 cut(s)	250, 1305
Ksp22I	TGATCA	2 cut(s)	22, 1056
Kzo9I	GATC	5 cut(s)	22, 267, 384, 922, 1056
LmnI	GCTCC	8 cut(s)	104, 156, 394, 729, 747, 908, 1153, 1473
Lsp1109I	GCAGC	3 cut(s)	279, 1287, 1478
LweI	GCATC	2 cut(s)	508, 849
MabI	ACCWGGT	1 cut(s)	487
MaeI	CTAG	3 cut(s)	179, 1070, 1495
MaeII	ACGT	4 cut(s)	242, 1109, 1222, 1316
MaeIII	GTNAC	7 cut(s)	169, 611, 646, 967, 1079, 1282, 1450
MalI	GATC	5 cut(s)	24, 269, 386, 924, 1058
MboI	GATC	5 cut(s)	22, 267, 384, 922, 1056
MboII	GAAGA	7 cut(s)	520, 670, 748, 886, 922, 1085, 1246
MhlI	GDGCHC	7 cut(s)	32, 790, 829, 857, 1042, 1271, 1429
MlsI	TGGCCA	1 cut(s)	281
MluCI	AATT	2 cut(s)	596, 1351
MluNI	TGGCCA	1 cut(s)	281
MlyI	GAGTC	3 cut(s)	180, 281, 335
MmeI	TCCRAC	1 cut(s)	1495
Mox20I	TGGCCA	1 cut(s)	281
MroI	TCCGGA	1 cut(s)	1096
MroNI	GCCGGC	2 cut(s)	248, 1303
MscI	TGGCCA	1 cut(s)	281
MseI	TTAA	1 cut(s)	525
MslI	CAYNNNNRTG	1 cut(s)	1225
Msp20I	TGGCCA	1 cut(s)	281
MspCI	CTTAAG	1 cut(s)	524
MvaI	CCWGG	3 cut(s)	489, 1085, 1408
MvnI	CGCG	3 cut(s)	336, 1032, 1151
MwoI	GCNNNNNNNGC	8 cut(s)	122, 245, 667, 824, 909, 1029, 1046, 1148
NaeI	GCCGGC	2 cut(s)	250, 1305
NciI	CCSGG	7 cut(s)	784, 1037, 1175, 1271, 1327, 1399, 1424
NdeII	GATC	5 cut(s)	22, 267, 384, 922, 1056
NgoMIV	GCCGGC	2 cut(s)	248, 1303
NmeAIII	GCCGAG	2 cut(s)	810, 927
NmuCI	GTSAC	4 cut(s)	967, 1079, 1282, 1450
NspI	RCATGY	2 cut(s)	351, 1204
OliI	CACNNNNGTG	1 cut(s)	1225
PaeI	GCATGC	1 cut(s)	351
PaeR7I	CTCGAG	1 cut(s)	307
PagI	TCATGA	2 cut(s)	591, 1257
PcsI	WCGNNNNNNNCGW	1 cut(s)	446
PdiI	GCCGGC	2 cut(s)	250, 1305
PfeI	GAWTC	5 cut(s)	142, 209, 219, 420, 1134
Pfl23II	CGTACG	1 cut(s)	1275
PflMI	CCANNNNNTGG	2 cut(s)	557, 1345
PkrI	GCNGC	6 cut(s)	294, 953, 1051, 1302, 1375, 1493
PleI	GAGTC	3 cut(s)	180, 280, 334
PmaCI	CACGTG	2 cut(s)	1110, 1223
PmlI	CACGTG	2 cut(s)	1110, 1223
PpsI	GAGTC	3 cut(s)	180, 280, 334
Ppu21I	YACGTR	2 cut(s)	1110, 1223
PshAI	GACNNNNGTC	1 cut(s)	690
PsiI	TTATAA	1 cut(s)	456
Psp1406I	AACGTT	1 cut(s)	242
Psp6I	CCWGG	3 cut(s)	487, 1083, 1406
PspCI	CACGTG	2 cut(s)	1110, 1223
PspEI	GGTNACC	2 cut(s)	1079, 1282
PspGI	CCWGG	3 cut(s)	487, 1083, 1406
PspLI	CGTACG	1 cut(s)	1275
PspOMI	GGGCCC	2 cut(s)	825, 1038
PspPI	GGNCC	9 cut(s)	825, 826, 1038, 1039, 1171, 1212, 1240, 1323, 1446
PspXI	VCTCGAGB	1 cut(s)	307
RsaI	GTAC	2 cut(s)	1277, 1421
RsaNI	GTAC	2 cut(s)	1276, 1420
RseI	CAYNNNNRTG	1 cut(s)	1225
SaqAI	TTAA	1 cut(s)	525
SatI	GCNGC	6 cut(s)	293, 952, 1050, 1301, 1374, 1492
Sau3AI	GATC	5 cut(s)	22, 267, 384, 922, 1056
Sau96I	GGNCC	9 cut(s)	825, 826, 1038, 1039, 1171, 1212, 1240, 1323, 1446
SchI	GAGTC	3 cut(s)	180, 281, 335
SduI	GDGCHC	7 cut(s)	32, 790, 829, 857, 1042, 1271, 1429
SexAI	ACCWGGT	1 cut(s)	487
SfaNI	GCATC	2 cut(s)	508, 849
Sfr274I	CTCGAG	1 cut(s)	307
SgrAI	CRCCGGYG	1 cut(s)	884
SinI	GGWCC	4 cut(s)	1171, 1212, 1240, 1446
SlaI	CTCGAG	1 cut(s)	307
SmiMI	CAYNNNNRTG	1 cut(s)	1225
SmlI	CTYRAG	2 cut(s)	307, 524
SmoI	CTYRAG	2 cut(s)	307, 524
SphI	GCATGC	1 cut(s)	351
Sse9I	AATT	2 cut(s)	596, 1351
SspMI	CTAG	3 cut(s)	179, 1070, 1495
TaaI	ACNGT	6 cut(s)	19, 58, 430, 847, 1018, 1450
TaiI	ACGT	4 cut(s)	245, 1112, 1225, 1319
TaqI	TCGA	6 cut(s)	270, 308, 423, 921, 1044, 1334
TasI	AATT	2 cut(s)	596, 1351
TauI	GCSGC	3 cut(s)	954, 1052, 1376
TfiI	GAWTC	5 cut(s)	142, 209, 219, 420, 1134
Tru1I	TTAA	1 cut(s)	525
Tru9I	TTAA	1 cut(s)	525
TscAI	CASTG	2 cut(s)	32, 583
TseFI	GTSAC	4 cut(s)	967, 1079, 1282, 1450
TseI	GCWGC	3 cut(s)	292, 1300, 1491
Tsp45I	GTSAC	4 cut(s)	967, 1079, 1282, 1450
TspDTI	ATGAA	7 cut(s)	580, 608, 886, 988, 1246, 1274, 1404
TspGWI	ACGGA	5 cut(s)	265, 455, 761, 967, 1175
TspRI	CASTG	2 cut(s)	32, 583
Van91I	CCANNNNNTGG	2 cut(s)	557, 1345
Vha464I	CTTAAG	1 cut(s)	524
VneI	GTGCAC	1 cut(s)	28
VpaK11BI	GGWCC	4 cut(s)	1171, 1212, 1240, 1446
XbaI	TCTAGA	1 cut(s)	1069
XceI	RCATGY	2 cut(s)	351, 1204
XcmI	CCANNNNNNNNNTGG	2 cut(s)	554, 1483
XhoI	CTCGAG	1 cut(s)	307
XmiI	GTMKAC	1 cut(s)	810
XspI	CTAG	3 cut(s)	179, 1070, 1495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.