Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0001249

Physical Location & Seq

Genomic Coordinates Forward (+)

53198520 .. 53205231

Gene Structure 6712 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0359500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGCCGGCCAAGGACGACGCCGCCGCCGCCGCCGAGAAGGAGAAGAACCTTGAGCTCTTCCTGAAGGTCGGACTGGATGAGCGGACGGCCAAAAACACCATCGCCAACAACAAGGTCACGGCGAATCTAACGGCCGTCATTGACGAGGCTGGAGTTACTGATGGATGCAGTCGAAGCGTTGGTAATCTTCTATACACGGTTGCTACAAAGTTTCCGGCAAATGCCCTTGTGCATCGCCCCGTGTTAACACAGTACATTGTGTCTTCAAAGATTAAAATCCCGGCTCAGTTAGATGCGGCTTTGTCATATTTTGCGAAAACGGGGCCAGAAAACTTCAAAACAACTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAACAGAATAAGTCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCCTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGATACAAACTTGCGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGCTATTGCTGTTTGTACTCCTGAACAACCAGCTGAAGAAATTCTAAATCCATATGCAATATTCCCTCAGCCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAGCACTTGAGTGTGACTGGGGGCAGAGTTTTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATCGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCCGGAAGCTGAAAAGAAGGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACAGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCACTGAAACTTTTTGAGGATATGAGGCGAGGCCTGATTGAAGAAGGGAAAGCAACTCTAAGAATGAAACAAGATATGCAGAGTGATAATTTTAACATGTATGACCTTATTGCGTATCGTATCAAGTTTACTCCTCATCCACATGCTGGAGACAAGTGGTGTATCTATCCGAGTTACGATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATCACCCATTCGCTGTGCACTTTAGAATTTGAGACACGCCGGGCTTCATACTACTGGCTATTGCATGCTTTGGGCCTTTATCAACCATATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAGCGTAAGTTAAATCGACTGGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTATGACACTGGCTGGTTTACGACGTAGGGGTGTGACGTCGACTGCCATTAATACGTTTGTTAGAGGAATTGGAATCACCAGAAGTGATTGTAGTATGATTCATCTGAGTCGCCTTGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCACGCACAATGGTTGTGCTTGATCCTGTAAAGGTTGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGATTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGATTACTTCGGACTTGCTCCTGGAAAATCTGCCCTGCTCCGATACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTGTTTTAGCTGAGGATGATGAGACTGTCCTTGAGATACGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCTGGGGTCGATCCTCTCAAAGTGGAAGTCCGATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCGAAAGTGGTAATACCAAATGCATTTTCTGTTCCCCCACTTGGGAAAGGTGTTGGTGTTGGCGACAAGTTTCAGTTTGAAAGACTTGGGTATTTTGTGGTCGACAAAGATACGACTCCTGAGAAGCTTGTGTTTAATCGTACAGTCACGCTGCGAGATAGCTACAAGAGTGGGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009791 GO:0009908 GO:0009987 GO:0010143 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0043170 GO:0044237 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090447 GO:0090567 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

796

Amino Acids

90.9

Weight (kDa)

6.29

Isoelectric Point (pI)

37.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_synt_1c_R1	PF04558	15 - 172	1e-55	Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2	PF04557	175 - 250	8.4e-07	Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c	PF00749	274 - 578	4.6e-122	tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C	PF03950	581 - 684	2.8e-18	tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2	PF20974	694 - 772	8.8e-15	tRNA synthetases class I (E and Q), anti-codon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001249)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g08570 FvH4_4g03930 FvH4_4g35510 FvH4_6g49610
malus_domestica	MD09G1043100.v1.1 MD12G1162100.v1.1 MD13G1082600.v1.1 MD16G1081700.v1.1 MD17G1044600.v1.1
prunus_persica	Prupe.1G264300_v2.0.a1 Prupe.3G274600_v2.0.a1
pyrus_communis	pycom111g03370 pycom16g07050
rosa_chinensis	RchiOBHm_Chr2g0169641 RchiOBHm_Chr4g0393951 RchiOBHm_Chr4g0445161 RchiOBHm_Chr6g0263921
rosa_laevigata	RLG00000005762 RLG00000009723 RLG00000014303 RLG00000021890
rosa_multiflora	Rmu_co8152054.1_g000001 Rmu_co8379785.1_g000001 Rmu_sc0000050.1_g000041 Rmu_sc0000363.1_g000001 Rmu_sc0002112.1_g000017 Rmu_sc0002720.1_g000011 Rmu_sc0013280.1_g000008
rosa_roxburghii	Rroxscaffold_176G00431300 Rroxscaffold_2G00081930 Rroxscaffold_7G00203770
rosa_rugosa	Rorug02G0541700 Rorug03G0369800.1 Rorug04G0359500 Rorug06G0006900
rosa_samantha	Rh2AG614100 Rh2BG626300 Rh2CG595600 Rh2DG637200 Rh4AG050000 Rh4AG420300 Rh4BG046400 Rh4BG432100 Rh4CG053400 Rh4CG446700 Rh4DG047000 Rh4DG428200 Rh6AG127000 Rh6BG123200 Rh6CG121700 Rh6DG109100
rosa_wichuraiana	Rw2G050890 Rw4G003950 Rw4G018340 Rw4G036040 Rw6G011030

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1631
AccB7I	CCANNNNNTGG	1 cut(s)	1328
AccBSI	CCGCTC	1 cut(s)	82
AccI	GTMKAC	2 cut(s)	1631, 2313
AccIII	TCCGGA	2 cut(s)	949, 2053
AciI	CCGC	7 cut(s)	21, 24, 27, 30, 82, 296, 1847
AclWI	GGATC	4 cut(s)	1577, 1760, 2045, 2111
AcoI	YGGCCR	3 cut(s)	6, 87, 132
AcsI	RAATTY	3 cut(s)	347, 675, 1421
AcuI	CTGAAG	3 cut(s)	83, 690, 717
AcyI	GRCGYC	2 cut(s)	18, 1628
AfaI	GTAC	6 cut(s)	254, 652, 1133, 1712, 1883, 2353
AfeI	AGCGCT	2 cut(s)	452, 1892
AfiI	CCNNNNNNNGG	7 cut(s)	874, 1162, 1328, 1810, 2108, 2252, 2253
AflIII	ACRYGT	1 cut(s)	1278
AhdI	GACNNNNNGTC	1 cut(s)	1508
AhlI	ACTAGT	1 cut(s)	1034
AjnI	CCWGG	4 cut(s)	503, 897, 1939, 2107
AjuI	GAANNNNNNNTTGG	2 cut(s)	2221, 2253
AleI	CACNNNNGTG	1 cut(s)	801
Alw21I	GWGCWC	4 cut(s)	57, 1077, 1415, 1731
Alw26I	GTCTC	4 cut(s)	490, 1326, 1421, 2015
Alw44I	GTGCAC	1 cut(s)	1411
AlwI	GGATC	4 cut(s)	1577, 1760, 2045, 2111
Aor13HI	TCCGGA	2 cut(s)	949, 2053
Aor51HI	AGCGCT	2 cut(s)	452, 1892
ApaLI	GTGCAC	1 cut(s)	1411
ApeKI	GCWGC	5 cut(s)	448, 575, 921, 2083, 2362
ApoI	RAATTY	3 cut(s)	347, 675, 1421
ArsI	GACNNNNNNTTYG	2 cut(s)	2118, 2150
AseI	ATTAAT	1 cut(s)	1641
Asp700I	GAANNNNTTC	2 cut(s)	591, 675
AspLEI	GCGC	2 cut(s)	453, 1893
AspS9I	GGNCC	3 cut(s)	323, 1468, 1833
AsuC2I	CCSGG	2 cut(s)	281, 1436
AsuHPI	GGTGA	5 cut(s)	482, 575, 1390, 1564, 1660
BaeGI	GKGCMC	1 cut(s)	1415
BaeI	ACNNNNGTAYC	2 cut(s)	1954, 1987
BanII	GRGCYC	4 cut(s)	57, 1017, 1077, 1731
BbsI	GAAGAC	2 cut(s)	255, 2069
Bbv12I	GWGCWC	4 cut(s)	57, 1077, 1415, 1731
BbvCI	CCTCAGC	2 cut(s)	702, 2010
BbvI	GCAGC	5 cut(s)	460, 587, 908, 2070, 2349
BccI	CCATC	5 cut(s)	107, 155, 743, 997, 1565
BceAI	ACGGC	4 cut(s)	102, 119, 135, 147
BciT130I	CCWGG	4 cut(s)	505, 899, 1941, 2109
BclI	TGATCA	2 cut(s)	976, 1102
BcnI	CCSGG	2 cut(s)	281, 1436
BcoDI	GTCTC	4 cut(s)	490, 1326, 1421, 2015
BcuI	ACTAGT	1 cut(s)	1034
BfaI	CTAG	1 cut(s)	1035
BfmI	CTRYAG	1 cut(s)	573
BfoI	RGCGCY	2 cut(s)	454, 1894
BglII	AGATCT	1 cut(s)	1051
BlpI	GCTNAGC	1 cut(s)	2175
Bme1390I	CCNGG	6 cut(s)	281, 505, 899, 1436, 1941, 2109
BmeRI	GACNNNNNGTC	1 cut(s)	1508
BmgT120I	GGNCC	3 cut(s)	323, 1468, 1833
BmiI	GGNNCC	4 cut(s)	324, 594, 1014, 1863
BmrFI	CCNGG	6 cut(s)	281, 505, 899, 1436, 1941, 2109
BmrI	ACTGGG	1 cut(s)	819
BmsI	GCATC	5 cut(s)	155, 241, 283, 539, 1978
BmuI	ACTGGG	1 cut(s)	819
BpiI	GAAGAC	2 cut(s)	255, 2069
BplI	GAGNNNNNCTC	2 cut(s)	1713, 1745
BpmI	CTGGAG	2 cut(s)	171, 1350
Bpu10I	CCTNAGC	2 cut(s)	702, 2010
Bpu1102I	GCTNAGC	1 cut(s)	2175
BpuEI	CTTGAG	5 cut(s)	71, 820, 1409, 1727, 2051
BpuMI	CCSGG	2 cut(s)	281, 1436
BsaBI	GATNNNNATC	1 cut(s)	275
BsaHI	GRCGYC	2 cut(s)	18, 1628
BsaJI	CCNNGG	7 cut(s)	9, 503, 504, 873, 1157, 1872, 2108
BsaWI	WCCGGW	2 cut(s)	949, 2053
Bsc4I	CCNNNNNNNGG	7 cut(s)	874, 1162, 1328, 1810, 2108, 2252, 2253
Bse118I	RCCGGY	1 cut(s)	4
Bse1I	ACTGG	7 cut(s)	78, 814, 1454, 1554, 1605, 1796, 2192
Bse3DI	GCAATG	2 cut(s)	885, 1369
Bse8I	GATNNNNATC	1 cut(s)	275
BseAI	TCCGGA	2 cut(s)	949, 2053
BseBI	CCWGG	4 cut(s)	505, 899, 1941, 2109
BseDI	CCNNGG	7 cut(s)	9, 503, 504, 873, 1157, 1872, 2108
BseJI	GATNNNNATC	1 cut(s)	275
BseLI	CCNNNNNNNGG	7 cut(s)	874, 1162, 1328, 1810, 2108, 2252, 2253
BseMI	GCAATG	2 cut(s)	885, 1369
BseNI	ACTGG	7 cut(s)	78, 814, 1454, 1554, 1605, 1796, 2192
BseRI	GAGGAG	1 cut(s)	1305
BseSI	GKGCMC	1 cut(s)	1415
BseX3I	CGGCCG	1 cut(s)	132
BseXI	GCAGC	5 cut(s)	460, 587, 908, 2070, 2349
Bsh1285I	CGRYCG	1 cut(s)	135
BsiEI	CGRYCG	1 cut(s)	135
BsiHKAI	GWGCWC	4 cut(s)	57, 1077, 1415, 1731
BsiSI	CCGG	6 cut(s)	5, 215, 281, 950, 1435, 2054
BslFI	GGGAC	1 cut(s)	1178
BslI	CCNNNNNNNGG	7 cut(s)	874, 1162, 1328, 1810, 2108, 2252, 2253
BsmAI	GTCTC	4 cut(s)	490, 1326, 1421, 2015
BsmFI	GGGAC	1 cut(s)	1178
Bsp1286I	GDGCHC	5 cut(s)	57, 1017, 1077, 1415, 1731
Bsp13I	TCCGGA	2 cut(s)	949, 2053
Bsp1407I	TGTACA	1 cut(s)	1881
Bsp143I	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
Bsp1720I	GCTNAGC	1 cut(s)	2175
BspACI	CCGC	7 cut(s)	21, 24, 27, 30, 82, 296, 1847
BspEI	TCCGGA	2 cut(s)	949, 2053
BspLI	GGNNCC	4 cut(s)	324, 594, 1014, 1863
BspMAI	CTGCAG	1 cut(s)	577
BspPI	GGATC	4 cut(s)	1577, 1760, 2045, 2111
BspQI	GCTCTTC	2 cut(s)	62, 1719
BsrBI	CCGCTC	1 cut(s)	82
BsrDI	GCAATG	2 cut(s)	885, 1369
BsrFI	RCCGGY	1 cut(s)	4
BsrGI	TGTACA	1 cut(s)	1881
BsrI	ACTGG	7 cut(s)	78, 814, 1454, 1554, 1605, 1796, 2192
BssAI	RCCGGY	1 cut(s)	4
BssECI	CCNNGG	7 cut(s)	9, 503, 504, 873, 1157, 1872, 2108
BssMI	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
BssNI	GRCGYC	2 cut(s)	18, 1628
BssT1I	CCWWGG	4 cut(s)	9, 873, 1157, 1872
Bst2UI	CCWGG	4 cut(s)	505, 899, 1941, 2109
Bst4CI	ACNGT	6 cut(s)	199, 252, 1522, 1817, 2026, 2356
Bst6I	CTCTTC	3 cut(s)	62, 488, 1719
BstACI	GRCGYC	2 cut(s)	18, 1628
BstAUI	TGTACA	1 cut(s)	1881
BstC8I	GCNNGC	3 cut(s)	6, 618, 1461
BstENI	CCTNNNNNAGG	1 cut(s)	1808
BstH2I	RGCGCY	2 cut(s)	454, 1894
BstHHI	GCGC	2 cut(s)	453, 1893
BstKTI	GATC	9 cut(s)	979, 1026, 1054, 1105, 1585, 1768, 2053, 2119, 2200
BstMAI	GTCTC	4 cut(s)	490, 1326, 1421, 2015
BstMBI	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
BstMCI	CGRYCG	1 cut(s)	135
BstMWI	GCNNNNNNNGC	3 cut(s)	26, 29, 174
BstNI	CCWGG	4 cut(s)	505, 899, 1941, 2109
BstNSI	RCATGY	5 cut(s)	363, 1282, 1328, 1463, 1971
BstSCI	CCNGG	6 cut(s)	279, 503, 897, 1434, 1939, 2107
BstSFI	CTRYAG	1 cut(s)	573
BstSLI	GKGCMC	1 cut(s)	1415
BstV1I	GCAGC	5 cut(s)	460, 587, 908, 2070, 2349
BstV2I	GAAGAC	2 cut(s)	255, 2069
BstX2I	RGATCY	1 cut(s)	1051
BstXI	CCANNNNNNTGG	1 cut(s)	1488
BstYI	RGATCY	1 cut(s)	1051
BstZI	CGGCCG	1 cut(s)	132
BtgZI	GCGATG	2 cut(s)	85, 218
BtsI	GCAGTG	1 cut(s)	1074
BtsIMutI	CAGTG	4 cut(s)	1074, 1184, 1518, 1598
Cac8I	GCNNGC	3 cut(s)	6, 618, 1461
CfoI	GCGC	2 cut(s)	453, 1893
Cfr10I	RCCGGY	1 cut(s)	4
Cfr13I	GGNCC	3 cut(s)	323, 1468, 1833
CseI	GACGC	1 cut(s)	26
Csp6I	GTAC	6 cut(s)	253, 651, 1132, 1711, 1882, 2352
CviAII	CATG	6 cut(s)	360, 869, 1279, 1325, 1460, 1968
CviQI	GTAC	6 cut(s)	253, 651, 1132, 1711, 1882, 2352
DpnI	GATC	9 cut(s)	978, 1025, 1053, 1104, 1584, 1767, 2052, 2118, 2199
DpnII	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
DriI	GACNNNNNGTC	1 cut(s)	1508
EaeI	YGGCCR	3 cut(s)	6, 87, 132
EagI	CGGCCG	1 cut(s)	132
Eam1104I	CTCTTC	3 cut(s)	62, 488, 1719
Eam1105I	GACNNNNNGTC	1 cut(s)	1508
EarI	CTCTTC	3 cut(s)	62, 488, 1719
EciI	GGCGGA	1 cut(s)	1862
Ecl136II	GAGCTC	3 cut(s)	55, 1075, 1729
EclXI	CGGCCG	1 cut(s)	132
Eco130I	CCWWGG	4 cut(s)	9, 873, 1157, 1872
Eco147I	AGGCCT	1 cut(s)	1215
Eco24I	GRGCYC	4 cut(s)	57, 1017, 1077, 1731
Eco32I	GATATC	1 cut(s)	854
Eco47III	AGCGCT	2 cut(s)	452, 1892
Eco52I	CGGCCG	1 cut(s)	132
Eco53kI	GAGCTC	3 cut(s)	55, 1075, 1729
Eco57I	CTGAAG	3 cut(s)	83, 690, 717
EcoICRI	GAGCTC	3 cut(s)	55, 1075, 1729
EcoNI	CCTNNNNNAGG	1 cut(s)	1808
EcoRII	CCWGG	4 cut(s)	503, 897, 1939, 2107
EcoRV	GATATC	1 cut(s)	854
EcoT14I	CCWWGG	4 cut(s)	9, 873, 1157, 1872
EcoT22I	ATGCAT	1 cut(s)	2236
EcoT38I	GRGCYC	4 cut(s)	57, 1017, 1077, 1731
ErhI	CCWWGG	4 cut(s)	9, 873, 1157, 1872
FaeI	CATG	6 cut(s)	363, 872, 1282, 1328, 1463, 1971
FaqI	GGGAC	1 cut(s)	1178
FatI	CATG	6 cut(s)	359, 868, 1278, 1324, 1459, 1967
FauNDI	CATATG	2 cut(s)	688, 1483
FbaI	TGATCA	2 cut(s)	976, 1102
FblI	GTMKAC	2 cut(s)	1631, 2313
FriOI	GRGCYC	4 cut(s)	57, 1017, 1077, 1731
FspBI	CTAG	1 cut(s)	1035
GlaI	GCGC	2 cut(s)	452, 1892
GsuI	CTGGAG	2 cut(s)	171, 1350
HaeII	RGCGCY	2 cut(s)	454, 1894
HapII	CCGG	6 cut(s)	5, 215, 281, 950, 1435, 2054
HgaI	GACGC	1 cut(s)	26
HhaI	GCGC	2 cut(s)	453, 1893
Hin1I	GRCGYC	2 cut(s)	18, 1628
Hin1II	CATG	6 cut(s)	363, 872, 1282, 1328, 1463, 1971
Hin6I	GCGC	2 cut(s)	451, 1891
HinP1I	GCGC	2 cut(s)	451, 1891
HincII	GTYRAC	3 cut(s)	246, 1632, 2314
HindII	GTYRAC	3 cut(s)	246, 1632, 2314
HindIII	AAGCTT	1 cut(s)	2336
HpaI	GTTAAC	1 cut(s)	246
HpaII	CCGG	6 cut(s)	5, 215, 281, 950, 1435, 2054
HphI	GGTGA	5 cut(s)	482, 575, 1390, 1564, 1660
Hpy166II	GTNNAC	6 cut(s)	246, 1311, 1413, 1610, 1632, 2314
Hpy188III	TCNNGA	9 cut(s)	61, 656, 839, 950, 986, 1388, 1500, 2054, 2330
Hpy8I	GTNNAC	6 cut(s)	246, 1311, 1413, 1610, 1632, 2314
Hpy99I	CGWCG	3 cut(s)	20, 1617, 1633
HpyAV	CCTTC	6 cut(s)	31, 58, 958, 1077, 1220, 2112
HpyCH4III	ACNGT	6 cut(s)	199, 252, 1522, 1817, 2026, 2356
HpyCH4IV	ACGT	4 cut(s)	1098, 1615, 1628, 1646
HpyF10VI	GCNNNNNNNGC	3 cut(s)	26, 29, 174
HpySE526I	ACGT	4 cut(s)	1098, 1615, 1628, 1646
Hsp92I	GRCGYC	2 cut(s)	18, 1628
Hsp92II	CATG	6 cut(s)	363, 872, 1282, 1328, 1463, 1971
HspAI	GCGC	2 cut(s)	451, 1891
Kpn2I	TCCGGA	2 cut(s)	949, 2053
KroI	GCCGGC	1 cut(s)	4
KroNI	GCCGGC	1 cut(s)	6
Ksp22I	TGATCA	2 cut(s)	976, 1102
KspAI	GTTAAC	1 cut(s)	246
Kzo9I	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
LguI	GCTCTTC	2 cut(s)	62, 1719
LmnI	GCTCC	7 cut(s)	445, 1012, 1072, 1747, 1898, 1942, 1962
Lsp1109I	GCAGC	5 cut(s)	460, 587, 908, 2070, 2349
LweI	GCATC	5 cut(s)	155, 241, 283, 539, 1978
MaeI	CTAG	1 cut(s)	1035
MaeII	ACGT	4 cut(s)	1098, 1615, 1628, 1646
MalI	GATC	9 cut(s)	978, 1025, 1053, 1104, 1584, 1767, 2052, 2118, 2199
MbiI	CCGCTC	1 cut(s)	82
MboI	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
MfeI	CAATTG	1 cut(s)	997
MflI	RGATCY	1 cut(s)	1051
MhlI	GDGCHC	5 cut(s)	57, 1017, 1077, 1415, 1731
MlyI	GAGTC	4 cut(s)	1190, 1708, 1907, 2320
MmeI	TCCRAC	1 cut(s)	49
Mph1103I	ATGCAT	1 cut(s)	2236
MroI	TCCGGA	2 cut(s)	949, 2053
MroNI	GCCGGC	1 cut(s)	4
MroXI	GAANNNNTTC	2 cut(s)	591, 675
MslI	CAYNNNNRTG	3 cut(s)	801, 1323, 1523
MspA1I	CMGCKG	1 cut(s)	668
MspI	CCGG	6 cut(s)	5, 215, 281, 950, 1435, 2054
MspR9I	CCNGG	6 cut(s)	281, 505, 899, 1436, 1941, 2109
MunI	CAATTG	1 cut(s)	997
MvaI	CCWGG	4 cut(s)	505, 899, 1941, 2109
MwoI	GCNNNNNNNGC	3 cut(s)	26, 29, 174
NaeI	GCCGGC	1 cut(s)	6
NciI	CCSGG	2 cut(s)	281, 1436
NdeI	CATATG	2 cut(s)	688, 1483
NdeII	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
NgoMIV	GCCGGC	1 cut(s)	4
NlaIII	CATG	6 cut(s)	363, 872, 1282, 1328, 1463, 1971
NlaIV	GGNNCC	4 cut(s)	324, 594, 1014, 1863
NmeAIII	GCCGAG	1 cut(s)	58
NmuCI	GTSAC	8 cut(s)	115, 470, 805, 1088, 1182, 1552, 1624, 2356
NsiI	ATGCAT	1 cut(s)	2236
NspI	RCATGY	5 cut(s)	363, 1282, 1328, 1463, 1971
OliI	CACNNNNGTG	1 cut(s)	801
PaeI	GCATGC	1 cut(s)	1463
PasI	CCCWGGG	1 cut(s)	504
PceI	AGGCCT	1 cut(s)	1215
PciI	ACATGT	1 cut(s)	1278
PciSI	GCTCTTC	2 cut(s)	62, 1719
PdiI	GCCGGC	1 cut(s)	6
PdmI	GAANNNNTTC	2 cut(s)	591, 675
PfeI	GAWTC	7 cut(s)	124, 1382, 1665, 1690, 1838, 1850, 2167
PflMI	CCANNNNNTGG	1 cut(s)	1328
PfoI	TCCNGGA	1 cut(s)	1939
PleI	GAGTC	4 cut(s)	1189, 1707, 1907, 2320
PpsI	GAGTC	4 cut(s)	1189, 1707, 1907, 2320
PscI	ACATGT	1 cut(s)	1278
PshBI	ATTAAT	1 cut(s)	1641
Psp124BI	GAGCTC	3 cut(s)	57, 1077, 1731
Psp6I	CCWGG	4 cut(s)	503, 897, 1939, 2107
PspGI	CCWGG	4 cut(s)	503, 897, 1939, 2107
PspN4I	GGNNCC	4 cut(s)	324, 594, 1014, 1863
PspPI	GGNCC	3 cut(s)	323, 1468, 1833
PstI	CTGCAG	1 cut(s)	577
PsuI	RGATCY	1 cut(s)	1051
PvuII	CAGCTG	1 cut(s)	668
RsaI	GTAC	6 cut(s)	254, 652, 1133, 1712, 1883, 2353
RsaNI	GTAC	6 cut(s)	253, 651, 1132, 1711, 1882, 2352
RseI	CAYNNNNRTG	3 cut(s)	801, 1323, 1523
SacI	GAGCTC	3 cut(s)	57, 1077, 1731
SalI	GTCGAC	2 cut(s)	1630, 2312
SapI	GCTCTTC	2 cut(s)	62, 1719
Sau3AI	GATC	9 cut(s)	976, 1023, 1051, 1102, 1582, 1765, 2050, 2116, 2197
Sau96I	GGNCC	3 cut(s)	323, 1468, 1833
SchI	GAGTC	4 cut(s)	1190, 1708, 1907, 2320
ScrFI	CCNGG	6 cut(s)	281, 505, 899, 1436, 1941, 2109
SduI	GDGCHC	5 cut(s)	57, 1017, 1077, 1415, 1731
SfaNI	GCATC	5 cut(s)	155, 241, 283, 539, 1978
SfcI	CTRYAG	1 cut(s)	573
SmiMI	CAYNNNNRTG	3 cut(s)	801, 1323, 1523
SmlI	CTYRAG	5 cut(s)	50, 799, 1388, 1706, 2030
SmoI	CTYRAG	5 cut(s)	50, 799, 1388, 1706, 2030
SpeI	ACTAGT	1 cut(s)	1034
SphI	GCATGC	1 cut(s)	1463
SseBI	AGGCCT	1 cut(s)	1215
SsiI	CCGC	7 cut(s)	21, 24, 27, 30, 82, 296, 1847
SspI	AATATT	2 cut(s)	696, 1496
SspMI	CTAG	1 cut(s)	1035
SstI	GAGCTC	3 cut(s)	57, 1077, 1731
StuI	AGGCCT	1 cut(s)	1215
StyD4I	CCNGG	6 cut(s)	279, 503, 897, 1434, 1939, 2107
StyI	CCWWGG	4 cut(s)	9, 873, 1157, 1872
TaaI	ACNGT	6 cut(s)	199, 252, 1522, 1817, 2026, 2356
TaiI	ACGT	4 cut(s)	1101, 1618, 1631, 1649
TaqI	TCGA	9 cut(s)	172, 888, 1529, 1546, 1631, 2043, 2115, 2213, 2313
TatI	WGTACW	4 cut(s)	252, 650, 1131, 1881
TauI	GCSGC	5 cut(s)	23, 26, 29, 32, 299
TfiI	GAWTC	7 cut(s)	124, 1382, 1665, 1690, 1838, 1850, 2167
TscAI	CASTG	4 cut(s)	1074, 1191, 1525, 1605
TseFI	GTSAC	8 cut(s)	115, 470, 805, 1088, 1182, 1552, 1624, 2356
TseI	GCWGC	5 cut(s)	448, 575, 921, 2083, 2362
Tsp45I	GTSAC	8 cut(s)	115, 470, 805, 1088, 1182, 1552, 1624, 2356
TspDTI	ATGAA	7 cut(s)	600, 716, 1074, 1163, 1262, 1431, 1682
TspRI	CASTG	4 cut(s)	1074, 1191, 1525, 1605
Van91I	CCANNNNNTGG	1 cut(s)	1328
VneI	GTGCAC	1 cut(s)	1411
VspI	ATTAAT	1 cut(s)	1641
XagI	CCTNNNNNAGG	1 cut(s)	1808
XapI	RAATTY	3 cut(s)	347, 675, 1421
XceI	RCATGY	5 cut(s)	363, 1282, 1328, 1463, 1971
XmiI	GTMKAC	2 cut(s)	1631, 2313
XmnI	GAANNNNTTC	2 cut(s)	591, 675
XspI	CTAG	1 cut(s)	1035
ZraI	GACGTC	1 cut(s)	1629
Zsp2I	ATGCAT	1 cut(s)	2236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.