Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004539

Physical Location & Seq

Genomic Coordinates Forward (+)

3134375 .. 3136032

Gene Structure 1658 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1047800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGGAGTTCATGTTGGTGAAGAATGAAGAGATCGCAGGGAGGCGCCATCTGAGCACAGCAGATCCGGAGGCTGAGGCGGCCCGGTGTGTCAAGAGAAGGAGAAGAGACCCGCCTGCAGTTGGTGGCAACAGAGAGAGTCAGCAGCAGTTGGCACAAAAGCCGGCTGATCAAACTTCTGCTGCCACCACAGTAAAGAGAAGCTCGAGATTTCGCGGTGTCAGCAGGCATCGGTGGACTGGTCGATTCGAAGCTCACTTGTGGGACAAGCTATCTTGGAATTCAACACTGAAGAAGAAGGGGAAACAAGTTTATCTTGGAGCTTACGATGAAGAAGAATCTGCTGCAAGAGCATATGATCTAGCTGCACTTAAGTACTGGGGAGCATCGACTTTCACCAACTTTCCGATATCTGACTACGACAACGAAATCGAGATCATGCAATCTGTGACAAAAGAAGAGTATTTGGCCTCGTTAAGAAGAAAGAGCAGTGGATTTTCAAGAGGTGTATCGAAGTACAGAGGAGTTGCAAGGCACCATCACAACGGTAGATGGGAAGCGAGAATTGGAAGAGTTTTTGGAAATAAGTACCTTTACCTTGGCACTTACGGTACGCAAGAGGAAGCAGCTCGTGCCTATGACATTGCAGCAATTGAGTACAGAGGGATCAATGCCGTGACCAACTTTGACTTGAGTTCTTACATAACATGGTTGAAGCCAAGAGCAGACAACGTGATAGCTACTCCACAGGTACCAGAGGCAGTCACAAAGCCCAAGAATACACCATTCGCGGCCAGCAGCTACATCACAACCGAGGAATCTCCCTTCTCACTCACAAATCCTTTTGCCTCTGATTACGTACACTCTCCTCGAAAGCAAGAAGTTATCAGCAGCAGCAAATCATCTTCTCCCACCGCGCTTGGCCTACTCCTCCAATCTTCAGTATTCAGAGAACTGGTTCAGAAGAACTCAAGTTTCTCGGAGGATGATAGCACTGACGGTGAAGAACCAAAGAACCAACCGCAGGCTGATAGCGATGATGAGTTCGGTGGAATAATCTACAACGGAATTGGTGGCAGCCCTTTTGTTAGCTCTTCCAACACTGATGGAGACTTTACATGGAATAGCTTTACAAGCTCGATTTTCGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006109 GO:0006110 GO:0006140 GO:0006355 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009743 GO:0009744 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0019219 GO:0019220 GO:0019222 GO:0019432 GO:0030808 GO:0030811 GO:0031323 GO:0031326 GO:0031329 GO:0034285 GO:0042221 GO:0042325 GO:0043467 GO:0043470 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0048518 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051174 GO:0051193 GO:0051196 GO:0051252 GO:0060255 GO:0062012 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1900371 GO:1900542 GO:1901363 GO:1901576 GO:1901700 GO:1901957 GO:1901959 GO:1903506 GO:1903578 GO:2000112 GO:2001141 GO:2001169

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

383

Amino Acids

42.68

Weight (kDa)

8.38

Isoelectric Point (pI)

57.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AP2	PF00847	68 - 127	5.8e-12	AP2 domain
AP2	PF00847	170 - 221	1.4e-12	AP2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004539)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g31880 FvH4_6g49200
malus_domestica	MD09G1047600.v1.1 MD09G1047800.v1.1 MD13G1078300.v1.1 MD17G1047900.v1.1
prunus_persica	Prupe.1G268800_v2.0.a1 Prupe.3G271200_v2.0.a1 Prupe.3G271400_v2.0.a1
pyrus_communis	pycom17g04250 pycom17g04270
rosa_chinensis	RchiOBHm_Chr2g0169071 RchiOBHm_Chr4g0440541
rosa_laevigata	RLG00000006169 RLG00000021831
rosa_multiflora	Rmu_co8472159.1_g000001 Rmu_sc0016635.1_g000002 Rmu_sc0037701.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082490 Rroxscaffold_3G00226840 Rroxscaffold_5G00381370
rosa_rugosa	Rorug02G0536600 Rorug04G0326600
rosa_samantha	Rh2AG608500 Rh2BG621300 Rh2CG590100 Rh2DG632000 Rh4CG405600 Rh4DG384600
rosa_wichuraiana	Rw2G050450 Rw4G032380 Rw4G032480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	748
AccB1I	GGYRCC	3 cut(s)	42, 531, 748
AccII	CGCG	4 cut(s)	213, 788, 914, 1145
AccIII	TCCGGA	1 cut(s)	64
AciI	CCGC	6 cut(s)	77, 110, 213, 788, 912, 1019
AclWI	GGATC	2 cut(s)	56, 671
AcoI	YGGCCR	1 cut(s)	789
AcsI	RAATTY	1 cut(s)	277
AcuI	CTGAAG	2 cut(s)	308, 921
AcyI	GRCGYC	1 cut(s)	43
AfaI	GTAC	7 cut(s)	374, 515, 587, 610, 656, 750, 858
AfiI	CCNNNNNNNGG	2 cut(s)	119, 1021
AflII	CTTAAG	1 cut(s)	368
AgsI	TTSAA	3 cut(s)	282, 498, 712
AjuI	GAANNNNNNNTTGG	2 cut(s)	546, 578
Alw21I	GWGCWC	1 cut(s)	56
Alw26I	GTCTC	2 cut(s)	99, 1101
AlwI	GGATC	2 cut(s)	56, 671
Ama87I	CYCGRG	1 cut(s)	202
Aor13HI	TCCGGA	1 cut(s)	64
AoxI	GGCC	4 cut(s)	78, 465, 789, 919
ApoI	RAATTY	1 cut(s)	277
Asp700I	GAANNNNTTC	1 cut(s)	954
Asp718I	GGTACC	1 cut(s)	748
AspLEI	GCGC	2 cut(s)	45, 916
AspS9I	GGNCC	1 cut(s)	79
AsuC2I	CCSGG	1 cut(s)	82
AsuHPI	GGTGA	3 cut(s)	28, 385, 1010
AsuII	TTCGAA	1 cut(s)	246
AvaI	CYCGRG	1 cut(s)	202
BanI	GGYRCC	3 cut(s)	42, 531, 748
BauI	CACGAG	1 cut(s)	627
Bbv12I	GWGCWC	1 cut(s)	56
BbvCI	CCTCAGC	1 cut(s)	72
BccI	CCATC	4 cut(s)	54, 543, 543, 1097
BceAI	ACGGC	1 cut(s)	656
BclI	TGATCA	1 cut(s)	166
BcnI	CCSGG	1 cut(s)	82
BcoDI	GTCTC	2 cut(s)	99, 1101
BfaI	CTAG	1 cut(s)	359
BfmI	CTRYAG	1 cut(s)	114
BfoI	RGCGCY	1 cut(s)	46
BfrI	CTTAAG	1 cut(s)	368
BmcAI	AGTACT	1 cut(s)	374
Bme1390I	CCNGG	1 cut(s)	82
BmeT110I	CYCGRG	1 cut(s)	202
BmgT120I	GGNCC	1 cut(s)	79
BmiI	GGNNCC	3 cut(s)	44, 533, 750
BmrFI	CCNGG	1 cut(s)	82
BmrI	ACTGGG	1 cut(s)	385
BmsI	GCATC	2 cut(s)	235, 392
BmuI	ACTGGG	1 cut(s)	385
Bpu10I	CCTNAGC	1 cut(s)	72
Bpu14I	TTCGAA	1 cut(s)	246
BpuEI	CTTGAG	2 cut(s)	709, 952
BpuMI	CCSGG	1 cut(s)	82
BsaAI	YACGTR	1 cut(s)	856
BsaHI	GRCGYC	1 cut(s)	43
BsaI	GGTCTC	1 cut(s)	99
BsaJI	CCNNGG	2 cut(s)	595, 810
BsaWI	WCCGGW	1 cut(s)	64
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	2 cut(s)	119, 1021
Bse118I	RCCGGY	1 cut(s)	160
Bse1I	ACTGG	3 cut(s)	241, 380, 957
Bse3DI	GCAATG	1 cut(s)	639
BseAI	TCCGGA	1 cut(s)	64
BseDI	CCNNGG	2 cut(s)	595, 810
BseGI	GGATG	1 cut(s)	988
BseLI	CCNNNNNNNGG	2 cut(s)	119, 1021
BseMI	GCAATG	1 cut(s)	639
BseMII	CTCAG	2 cut(s)	41, 63
BseNI	ACTGG	3 cut(s)	241, 380, 957
BseRI	GAGGAG	3 cut(s)	534, 855, 917
BsgI	GTGCAG	1 cut(s)	348
Bsh1236I	CGCG	4 cut(s)	213, 788, 914, 1145
BshFI	GGCC	4 cut(s)	80, 467, 791, 921
BshNI	GGYRCC	3 cut(s)	42, 531, 748
BsiHKAI	GWGCWC	1 cut(s)	56
BsiHKCI	CYCGRG	1 cut(s)	202
BsiSI	CCGG	3 cut(s)	65, 82, 161
BslFI	GGGAC	1 cut(s)	275
BslI	CCNNNNNNNGG	2 cut(s)	119, 1021
BsmAI	GTCTC	2 cut(s)	99, 1101
BsmFI	GGGAC	1 cut(s)	275
BsnI	GGCC	4 cut(s)	80, 467, 791, 921
Bso31I	GGTCTC	1 cut(s)	99
BsoBI	CYCGRG	1 cut(s)	202
Bsp119I	TTCGAA	1 cut(s)	246
Bsp1286I	GDGCHC	1 cut(s)	56
Bsp13I	TCCGGA	1 cut(s)	64
Bsp143I	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
BspACI	CCGC	6 cut(s)	77, 110, 213, 788, 912, 1019
BspANI	GGCC	4 cut(s)	80, 467, 791, 921
BspCNI	CTCAG	2 cut(s)	42, 64
BspEI	TCCGGA	1 cut(s)	64
BspFNI	CGCG	4 cut(s)	213, 788, 914, 1145
BspLI	GGNNCC	3 cut(s)	44, 533, 750
BspMAI	CTGCAG	1 cut(s)	118
BspPI	GGATC	2 cut(s)	56, 671
BspQI	GCTCTTC	1 cut(s)	1096
BspT104I	TTCGAA	1 cut(s)	246
BspT107I	GGYRCC	3 cut(s)	42, 531, 748
BspTI	CTTAAG	1 cut(s)	368
BspTNI	GGTCTC	1 cut(s)	99
BsrDI	GCAATG	1 cut(s)	639
BsrFI	RCCGGY	1 cut(s)	160
BsrI	ACTGG	3 cut(s)	241, 380, 957
BssAI	RCCGGY	1 cut(s)	160
BssECI	CCNNGG	2 cut(s)	595, 810
BssMI	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
BssNI	GRCGYC	1 cut(s)	43
BssSI	CACGAG	1 cut(s)	627
BssT1I	CCWWGG	1 cut(s)	595
Bst2BI	CACGAG	1 cut(s)	627
Bst4CI	ACNGT	4 cut(s)	190, 545, 608, 998
Bst6I	CTCTTC	5 cut(s)	21, 97, 450, 562, 1096
BstACI	GRCGYC	1 cut(s)	43
BstAFI	CTTAAG	1 cut(s)	368
BstAPI	GCANNNNNTGC	1 cut(s)	629
BstBAI	YACGTR	1 cut(s)	856
BstBI	TTCGAA	1 cut(s)	246
BstC8I	GCNNGC	5 cut(s)	114, 162, 224, 793, 1023
BstDEI	CTNAG	2 cut(s)	50, 72
BstF5I	GGATG	1 cut(s)	988
BstFNI	CGCG	4 cut(s)	213, 788, 914, 1145
BstH2I	RGCGCY	1 cut(s)	46
BstHHI	GCGC	2 cut(s)	45, 916
BstKTI	GATC	6 cut(s)	33, 64, 169, 358, 435, 666
BstMAI	GTCTC	2 cut(s)	99, 1101
BstMBI	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
BstMWI	GCNNNNNNNGC	6 cut(s)	51, 77, 219, 347, 629, 1131
BstSCI	CCNGG	1 cut(s)	80
BstSFI	CTRYAG	1 cut(s)	114
BstSNI	TACGTA	1 cut(s)	856
BstUI	CGCG	4 cut(s)	213, 788, 914, 1145
BstX2I	RGATCY	1 cut(s)	61
BstYI	RGATCY	1 cut(s)	61
BsuRI	GGCC	4 cut(s)	80, 467, 791, 921
BtgZI	GCGATG	1 cut(s)	1047
BtsCI	GGATG	1 cut(s)	988
BtsI	GCAGTG	1 cut(s)	493
BtsIMutI	CAGTG	4 cut(s)	284, 493, 990, 1098
Cac8I	GCNNGC	5 cut(s)	114, 162, 224, 793, 1023
CfoI	GCGC	2 cut(s)	45, 916
Cfr10I	RCCGGY	1 cut(s)	160
Cfr13I	GGNCC	1 cut(s)	79
Csp6I	GTAC	7 cut(s)	373, 514, 586, 609, 655, 749, 857
CspCI	CAANNNNNGTGG	2 cut(s)	898, 933
CviAII	CATG	4 cut(s)	10, 436, 705, 1116
CviQI	GTAC	7 cut(s)	373, 514, 586, 609, 655, 749, 857
DdeI	CTNAG	2 cut(s)	50, 72
DinI	GGCGCC	1 cut(s)	44
DpnI	GATC	6 cut(s)	32, 63, 168, 357, 434, 665
DpnII	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
EaeI	YGGCCR	1 cut(s)	789
Eam1104I	CTCTTC	5 cut(s)	21, 97, 450, 562, 1096
EarI	CTCTTC	5 cut(s)	21, 97, 450, 562, 1096
Eco105I	TACGTA	1 cut(s)	856
Eco130I	CCWWGG	1 cut(s)	595
Eco31I	GGTCTC	1 cut(s)	99
Eco32I	GATATC	1 cut(s)	408
Eco57I	CTGAAG	2 cut(s)	308, 921
Eco88I	CYCGRG	1 cut(s)	202
EcoRI	GAATTC	1 cut(s)	277
EcoRV	GATATC	1 cut(s)	408
EcoT14I	CCWWGG	1 cut(s)	595
EgeI	GGCGCC	1 cut(s)	44
EheI	GGCGCC	1 cut(s)	44
ErhI	CCWWGG	1 cut(s)	595
FaeI	CATG	4 cut(s)	13, 439, 708, 1119
FaiI	YATR	8 cut(s)	11, 352, 354, 437, 636, 701, 706, 1117
FalI	AAGNNNNNCTT	2 cut(s)	297, 329
FaqI	GGGAC	1 cut(s)	275
FatI	CATG	4 cut(s)	9, 435, 704, 1115
FauI	CCCGC	1 cut(s)	117
FauNDI	CATATG	1 cut(s)	352
FbaI	TGATCA	1 cut(s)	166
FokI	GGATG	1 cut(s)	995
FspBI	CTAG	1 cut(s)	359
GlaI	GCGC	2 cut(s)	44, 915
HaeII	RGCGCY	1 cut(s)	46
HaeIII	GGCC	4 cut(s)	80, 467, 791, 921
HapII	CCGG	3 cut(s)	65, 82, 161
HhaI	GCGC	2 cut(s)	45, 916
Hin1I	GRCGYC	1 cut(s)	43
Hin1II	CATG	4 cut(s)	13, 439, 708, 1119
Hin6I	GCGC	2 cut(s)	43, 914
HinP1I	GCGC	2 cut(s)	43, 914
HinfI	GANTC	4 cut(s)	136, 243, 335, 815
HpaII	CCGG	3 cut(s)	65, 82, 161
HphI	GGTGA	3 cut(s)	28, 385, 1010
Hpy166II	GTNNAC	2 cut(s)	234, 859
Hpy188I	TCNGA	7 cut(s)	51, 405, 412, 850, 947, 960, 979
Hpy188III	TCNNGA	5 cut(s)	65, 91, 204, 430, 498
Hpy8I	GTNNAC	2 cut(s)	234, 859
HpyAV	CCTTC	3 cut(s)	90, 289, 832
HpyCH4III	ACNGT	4 cut(s)	190, 545, 608, 998
HpyCH4IV	ACGT	2 cut(s)	729, 855
HpyCH4V	TGCA	6 cut(s)	116, 344, 365, 439, 527, 644
HpyF10VI	GCNNNNNNNGC	6 cut(s)	51, 77, 219, 347, 629, 1131
HpyF3I	CTNAG	2 cut(s)	50, 72
HpySE526I	ACGT	2 cut(s)	729, 855
Hsp92I	GRCGYC	1 cut(s)	43
Hsp92II	CATG	4 cut(s)	13, 439, 708, 1119
HspAI	GCGC	2 cut(s)	43, 914
KasI	GGCGCC	1 cut(s)	42
Kpn2I	TCCGGA	1 cut(s)	64
KpnI	GGTACC	1 cut(s)	752
KroI	GCCGGC	1 cut(s)	160
KroNI	GCCGGC	1 cut(s)	162
Ksp22I	TGATCA	1 cut(s)	166
Kzo9I	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
LguI	GCTCTTC	1 cut(s)	1096
LmnI	GCTCC	2 cut(s)	317, 380
LweI	GCATC	2 cut(s)	235, 392
MaeI	CTAG	1 cut(s)	359
MaeII	ACGT	2 cut(s)	729, 855
MaeIII	GTNAC	3 cut(s)	445, 673, 760
MalI	GATC	6 cut(s)	32, 63, 168, 357, 434, 665
MboI	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
MfeI	CAATTG	1 cut(s)	648
MflI	RGATCY	1 cut(s)	61
MhlI	GDGCHC	1 cut(s)	56
MluCI	AATT	4 cut(s)	277, 561, 648, 1065
Mly113I	GGCGCC	1 cut(s)	43
MlyI	GAGTC	1 cut(s)	145
MmeI	TCCRAC	1 cut(s)	1119
MroI	TCCGGA	1 cut(s)	64
MroNI	GCCGGC	1 cut(s)	160
MroXI	GAANNNNTTC	1 cut(s)	954
MseI	TTAA	2 cut(s)	369, 473
MslI	CAYNNNNRTG	1 cut(s)	14
MspCI	CTTAAG	1 cut(s)	368
MspI	CCGG	3 cut(s)	65, 82, 161
MspR9I	CCNGG	1 cut(s)	82
MunI	CAATTG	1 cut(s)	648
MvnI	CGCG	4 cut(s)	213, 788, 914, 1145
MwoI	GCNNNNNNNGC	6 cut(s)	51, 77, 219, 347, 629, 1131
NaeI	GCCGGC	1 cut(s)	162
NarI	GGCGCC	1 cut(s)	43
NciI	CCSGG	1 cut(s)	82
NdeI	CATATG	1 cut(s)	352
NdeII	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
NgoMIV	GCCGGC	1 cut(s)	160
NlaIII	CATG	4 cut(s)	13, 439, 708, 1119
NlaIV	GGNNCC	3 cut(s)	44, 533, 750
NmuCI	GTSAC	3 cut(s)	445, 673, 760
NspV	TTCGAA	1 cut(s)	246
PaeR7I	CTCGAG	1 cut(s)	202
PciSI	GCTCTTC	1 cut(s)	1096
PcsI	WCGNNNNNNNCGW	1 cut(s)	1142
PdiI	GCCGGC	1 cut(s)	162
PdmI	GAANNNNTTC	1 cut(s)	954
PfeI	GAWTC	3 cut(s)	243, 335, 815
PleI	GAGTC	1 cut(s)	144
PluTI	GGCGCC	1 cut(s)	46
PpsI	GAGTC	1 cut(s)	144
Ppu21I	YACGTR	1 cut(s)	856
PspN4I	GGNNCC	3 cut(s)	44, 533, 750
PspPI	GGNCC	1 cut(s)	79
PstI	CTGCAG	1 cut(s)	118
PsuI	RGATCY	1 cut(s)	61
RsaI	GTAC	7 cut(s)	374, 515, 587, 610, 656, 750, 858
RsaNI	GTAC	7 cut(s)	373, 514, 586, 609, 655, 749, 857
RseI	CAYNNNNRTG	1 cut(s)	14
SapI	GCTCTTC	1 cut(s)	1096
SaqAI	TTAA	2 cut(s)	369, 473
Sau3AI	GATC	6 cut(s)	30, 61, 166, 355, 432, 663
Sau96I	GGNCC	1 cut(s)	79
ScaI	AGTACT	1 cut(s)	374
SchI	GAGTC	1 cut(s)	145
ScrFI	CCNGG	1 cut(s)	82
SduI	GDGCHC	1 cut(s)	56
SfaNI	GCATC	2 cut(s)	235, 392
SfcI	CTRYAG	1 cut(s)	114
SfoI	GGCGCC	1 cut(s)	44
Sfr274I	CTCGAG	1 cut(s)	202
SfuI	TTCGAA	1 cut(s)	246
SlaI	CTCGAG	1 cut(s)	202
SmiMI	CAYNNNNRTG	1 cut(s)	14
SmlI	CTYRAG	4 cut(s)	202, 368, 688, 967
SmoI	CTYRAG	4 cut(s)	202, 368, 688, 967
SnaBI	TACGTA	1 cut(s)	856
Sse9I	AATT	4 cut(s)	277, 561, 648, 1065
SsiI	CCGC	6 cut(s)	77, 110, 213, 788, 912, 1019
SspDI	GGCGCC	1 cut(s)	42
SspMI	CTAG	1 cut(s)	359
StyD4I	CCNGG	1 cut(s)	80
StyI	CCWWGG	1 cut(s)	595
TaaI	ACNGT	4 cut(s)	190, 545, 608, 998
TaiI	ACGT	2 cut(s)	732, 858
TaqI	TCGA	8 cut(s)	203, 241, 246, 386, 429, 509, 868, 1136
TasI	AATT	4 cut(s)	277, 561, 648, 1065
TatI	WGTACW	3 cut(s)	372, 513, 654
TauI	GCSGC	2 cut(s)	80, 791
TfiI	GAWTC	3 cut(s)	243, 335, 815
Tru1I	TTAA	2 cut(s)	369, 473
Tru9I	TTAA	2 cut(s)	369, 473
TscAI	CASTG	4 cut(s)	291, 493, 997, 1105
TseFI	GTSAC	3 cut(s)	445, 673, 760
Tsp45I	GTSAC	3 cut(s)	445, 673, 760
TspDTI	ATGAA	2 cut(s)	39, 342
TspGWI	ACGGA	1 cut(s)	1077
TspRI	CASTG	4 cut(s)	291, 493, 997, 1105
Vha464I	CTTAAG	1 cut(s)	368
XapI	RAATTY	1 cut(s)	277
XhoI	CTCGAG	1 cut(s)	202
XmnI	GAANNNNTTC	1 cut(s)	954
XspI	CTAG	1 cut(s)	359
ZrmI	AGTACT	1 cut(s)	374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.