MD09G1092500.v1.1

Formiminotransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001831
Physical Location & Seq
Reverse (-)
6787989 .. 6789349
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1092500.v1.1.491

Sequence Viewer

Length: 918 bp
ATGTTGAAGTCGATGCTTGGTTGCTGCAAGGTTTACATATCCGAAAGCAGAAACAGGGCAGCGCTGGAATCAGTCGAACGGGCTGCTAAGCTTTTCTCGGAAGCACCGATTGTGAATAAGTTCGAAGATGAAACGTACAACAGAGTTGGTTACACACTTGTCTCTACGTTGGCTCCAAAGCCGTCTGTAGATCCTAGTCCTTTGAAGATGGCAGTGCTTGCCATGGTCAAGGCTGCTTTCGAAACAATTGATCTCGAGTCACATTGTGGAAGTCATCCGCGGCTCGGAGTTGTGGATCATATATGCTTTCATCCCCTTCTCGGTGCTTCTTTGGAGCAAGTGGCTGGTGTTGCAAACTCTTTGGCTGCAGAAGTTGGCTCTAGTCTTCAAGTTCCTACTTTTCTCTATGGAGCTGCCCATGAAGAGGGAAGAACGCTTGATTCAGTCAGAAGAGAGTTAGGTTATTTCAAGCCGAATTCTAGCGGAGAACAATGGGTTGGGGGACCGAAATCAGATTACTTGGCGCTGAAGCCGGACAAGGGTCCCCCTCAAGTGACTCAAGGAAGGGGTGTCATTGTGATTGGAGCAACCCGGTGGGTTGATAACTATAATGTCCCGGTCATCTCTACTGATATTGCTGCTGTTCGTAGAATTGCAAAACGAGTGAGTGGCAGGGGAGGCGGACTTGCTTCGGTCCAAGCCATGGCACTTGCGCACGGTGAATCCATCATCGAAGTCGCTTGCAATTTGTTGGAACCAGAGAAGGTGCGAGGAGATAGGGTTCAACTTGAAGTTGAGAGGCTTGCAAAGGAAGAGGGTATGAGAGTTGGGAAAGGCTACTTCACTGATTTTTCTCAGGAAAGATTAATTGAAAGTTACTTGAAGTCGGATTTCAGTACAAACAAAATGTGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FTCD_N Yae1_N

Protein Analysis

306

Amino Acids

33.18

Weight (kDa)

7.01

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FTCD_N PF07837 8 - 197 5.9e-53 Formiminotransferase domain, N-terminal subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 714
AccB7I CCANNNNNTGG 1 cut(s) 703
AccII CGCG 1 cut(s) 280
AciI CCGC 4 cut(s) 278, 280, 483, 681
AclWI GGATC 2 cut(s) 185, 303
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 548
AdeI CACNNNGTG 1 cut(s) 266
AfaI GTAC 2 cut(s) 137, 898
AfeI AGCGCT 1 cut(s) 63
AfiI CCNNNNNNNGG 5 cut(s) 284, 320, 424, 539, 703
AgsI TTSAA 8 cut(s) 7, 205, 389, 469, 785, 791, 872, 883
AjuI GAANNNNNNNTTGG 2 cut(s) 480, 512
AloI GAACNNNNNNTCC 2 cut(s) 765, 797
AluBI AGCT 2 cut(s) 91, 413
AluI AGCT 2 cut(s) 91, 413
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 2 cut(s) 185, 303
Ama87I CYCGRG 1 cut(s) 254
Aor51HI AGCGCT 1 cut(s) 63
ApeKI GCWGC 7 cut(s) 24, 59, 83, 233, 365, 413, 638
ApoI RAATTY 1 cut(s) 475
AseI ATTAAT 1 cut(s) 866
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 3 cut(s) 64, 526, 715
AspS9I GGNCC 3 cut(s) 503, 542, 694
AsuC2I CCSGG 2 cut(s) 592, 617
AsuHPI GGTGA 1 cut(s) 731
AsuII TTCGAA 2 cut(s) 123, 240
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 3 cut(s) 503, 542, 694
BbsI GAAGAC 1 cut(s) 377
BbvI GCAGC 7 cut(s) 11, 70, 71, 220, 352, 400, 625
BccI CCATC 2 cut(s) 202, 734
BceAI ACGGC 1 cut(s) 166
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BcnI CCSGG 2 cut(s) 592, 617
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 4 cut(s) 195, 381, 480, 916
BfmI CTRYAG 2 cut(s) 186, 366
BfoI RGCGCY 2 cut(s) 65, 527
BisI GCNGC 8 cut(s) 25, 60, 84, 234, 281, 366, 414, 639
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 8 cut(s) 26, 61, 85, 235, 282, 367, 415, 640
Bme1390I CCNGG 2 cut(s) 592, 617
Bme18I GGWCC 3 cut(s) 503, 542, 694
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 3 cut(s) 503, 542, 694
BmiI GGNNCC 5 cut(s) 174, 504, 543, 544, 756
BmrFI CCNGG 2 cut(s) 592, 617
BmsI GCATC 1 cut(s) 3
BoxI GACNNNNGTC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 377
Bpu1102I GCTNAGC 1 cut(s) 87
Bpu14I TTCGAA 2 cut(s) 123, 240
BpuEI CTTGAG 2 cut(s) 534, 543
BpuMI CCSGG 2 cut(s) 592, 617
BsaJI CCNNGG 3 cut(s) 222, 278, 702
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 765, 795
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 320, 424, 539, 703
BseDI CCNNGG 3 cut(s) 222, 278, 702
BseGI GGATG 2 cut(s) 274, 310
BseLI CCNNNNNNNGG 5 cut(s) 284, 320, 424, 539, 703
BseMII CTCAG 1 cut(s) 869
BseRI GAGGAG 1 cut(s) 786
BseXI GCAGC 7 cut(s) 11, 70, 71, 220, 352, 400, 625
Bsh1236I CGCG 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 3 cut(s) 533, 592, 617
BslFI GGGAC 3 cut(s) 516, 528, 599
BslI CCNNNNNNNGG 5 cut(s) 284, 320, 424, 539, 703
BsmAI GTCTC 1 cut(s) 166
BsmFI GGGAC 3 cut(s) 516, 528, 599
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 2 cut(s) 123, 240
Bsp143I GATC 3 cut(s) 190, 250, 295
Bsp1720I GCTNAGC 1 cut(s) 87
Bsp19I CCATGG 2 cut(s) 222, 702
BspACI CCGC 4 cut(s) 278, 280, 483, 681
BspCNI CTCAG 1 cut(s) 868
BspFNI CGCG 1 cut(s) 280
BspLI GGNNCC 5 cut(s) 174, 504, 543, 544, 756
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 2 cut(s) 185, 303
BspT104I TTCGAA 2 cut(s) 123, 240
BssECI CCNNGG 3 cut(s) 222, 278, 702
BssMI GATC 3 cut(s) 190, 250, 295
BssT1I CCWWGG 2 cut(s) 222, 702
Bst4CI ACNGT 1 cut(s) 719
Bst6I CTCTTC 3 cut(s) 417, 445, 807
BstAPI GCANNNNNTGC 1 cut(s) 218
BstBI TTCGAA 2 cut(s) 123, 240
BstC8I GCNNGC 3 cut(s) 219, 742, 804
BstDEI CTNAG 2 cut(s) 87, 855
BstDSI CCRYGG 3 cut(s) 222, 278, 702
BstF5I GGATG 2 cut(s) 274, 310
BstFNI CGCG 1 cut(s) 280
BstH2I RGCGCY 2 cut(s) 65, 527
BstHHI GCGC 3 cut(s) 64, 526, 715
BstKTI GATC 3 cut(s) 193, 253, 298
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 3 cut(s) 190, 250, 295
BstMWI GCNNNNNNNGC 3 cut(s) 218, 350, 678
BstPAI GACNNNNGTC 1 cut(s) 540
BstSCI CCNGG 2 cut(s) 590, 615
BstSFI CTRYAG 2 cut(s) 186, 366
BstUI CGCG 1 cut(s) 280
BstV1I GCAGC 7 cut(s) 11, 70, 71, 220, 352, 400, 625
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BtgI CCRYGG 3 cut(s) 222, 278, 702
BtsCI GGATG 2 cut(s) 274, 310
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 2 cut(s) 219, 843
Cac8I GCNNGC 3 cut(s) 219, 742, 804
CfoI GCGC 3 cut(s) 64, 526, 715
Cfr13I GGNCC 3 cut(s) 503, 542, 694
Cfr42I CCGCGG 1 cut(s) 281
Csp6I GTAC 2 cut(s) 136, 897
CviAII CATG 3 cut(s) 223, 419, 703
CviQI GTAC 2 cut(s) 136, 897
DdeI CTNAG 2 cut(s) 87, 855
DpnI GATC 3 cut(s) 192, 252, 297
DpnII GATC 3 cut(s) 190, 250, 295
DraIII CACNNNGTG 1 cut(s) 266
Eam1104I CTCTTC 3 cut(s) 417, 445, 807
EarI CTCTTC 3 cut(s) 417, 445, 807
EciI GGCGGA 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 222, 702
Eco47I GGWCC 3 cut(s) 503, 542, 694
Eco47III AGCGCT 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 542
EcoRI GAATTC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 222, 702
ErhI CCWWGG 2 cut(s) 222, 702
FaeI CATG 3 cut(s) 226, 422, 706
FaqI GGGAC 3 cut(s) 516, 528, 599
FatI CATG 3 cut(s) 222, 418, 702
Fnu4HI GCNGC 8 cut(s) 25, 60, 84, 234, 281, 366, 414, 639
FokI GGATG 2 cut(s) 261, 297
Fsp4HI GCNGC 8 cut(s) 25, 60, 84, 234, 281, 366, 414, 639
FspBI CTAG 4 cut(s) 195, 381, 480, 916
FspI TGCGCA 1 cut(s) 714
GlaI GCGC 3 cut(s) 63, 525, 714
GluI GCNGC 8 cut(s) 25, 60, 84, 234, 281, 366, 414, 639
HaeII RGCGCY 2 cut(s) 65, 527
HapII CCGG 3 cut(s) 533, 592, 617
HhaI GCGC 3 cut(s) 64, 526, 715
Hin1II CATG 3 cut(s) 226, 422, 706
Hin6I GCGC 3 cut(s) 62, 524, 713
HinP1I GCGC 3 cut(s) 62, 524, 713
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 5 cut(s) 68, 257, 440, 556, 722
HpaII CCGG 3 cut(s) 533, 592, 617
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 6 cut(s) 43, 100, 287, 449, 514, 889
Hpy188III TCNNGA 2 cut(s) 254, 857
Hpy8I GTNNAC 1 cut(s) 34
HpyAV CCTTC 3 cut(s) 326, 558, 757
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 2 cut(s) 134, 167
HpyCH4V TGCA 6 cut(s) 27, 353, 368, 656, 744, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 350, 678
HpyF3I CTNAG 2 cut(s) 87, 855
HpySE526I ACGT 2 cut(s) 134, 167
Hsp92II CATG 3 cut(s) 226, 422, 706
HspAI GCGC 3 cut(s) 62, 524, 713
KflI GGGWCCC 1 cut(s) 542
KspI CCGCGG 1 cut(s) 281
Kzo9I GATC 3 cut(s) 190, 250, 295
LmnI GCTCC 4 cut(s) 178, 334, 410, 584
LpnPI CCDG 9 cut(s) 40, 50, 330, 546, 605, 630, 658, 771, 842
Lsp1109I GCAGC 7 cut(s) 11, 70, 71, 220, 352, 400, 625
LweI GCATC 1 cut(s) 3
MaeI CTAG 4 cut(s) 195, 381, 480, 916
MaeII ACGT 2 cut(s) 134, 167
MaeIII GTNAC 4 cut(s) 149, 258, 553, 875
MalI GATC 3 cut(s) 192, 252, 297
MboI GATC 3 cut(s) 190, 250, 295
MboII GAAGA 7 cut(s) 137, 217, 377, 434, 441, 462, 824
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 190
MluCI AATT 5 cut(s) 246, 475, 651, 745, 867
MlyI GAGTC 2 cut(s) 266, 550
MmeI TCCRAC 2 cut(s) 732, 867
MnlI CCTC 6 cut(s) 418, 558, 671, 764, 792, 808
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 3 cut(s) 533, 592, 617
MspR9I CCNGG 2 cut(s) 592, 617
MunI CAATTG 1 cut(s) 246
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 3 cut(s) 218, 350, 678
NciI CCSGG 2 cut(s) 592, 617
NcoI CCATGG 2 cut(s) 222, 702
NdeII GATC 3 cut(s) 190, 250, 295
NlaIII CATG 3 cut(s) 226, 422, 706
NlaIV GGNNCC 5 cut(s) 174, 504, 543, 544, 756
NmuCI GTSAC 2 cut(s) 258, 553
NsbI TGCGCA 1 cut(s) 714
NspV TTCGAA 2 cut(s) 123, 240
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 3 cut(s) 68, 440, 722
PflMI CCANNNNNTGG 1 cut(s) 703
PkrI GCNGC 8 cut(s) 26, 61, 85, 235, 282, 367, 415, 640
PleI GAGTC 2 cut(s) 265, 550
PpsI GAGTC 2 cut(s) 265, 550
PpuMI RGGWCCY 1 cut(s) 542
PshAI GACNNNNGTC 1 cut(s) 540
PshBI ATTAAT 1 cut(s) 866
Psp5II RGGWCCY 1 cut(s) 542
PspN4I GGNNCC 5 cut(s) 174, 504, 543, 544, 756
PspPI GGNCC 3 cut(s) 503, 542, 694
PspPPI RGGWCCY 1 cut(s) 542
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 2 cut(s) 137, 898
RsaNI GTAC 2 cut(s) 136, 897
SacII CCGCGG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 866
SatI GCNGC 8 cut(s) 25, 60, 84, 234, 281, 366, 414, 639
Sau3AI GATC 3 cut(s) 190, 250, 295
Sau96I GGNCC 3 cut(s) 503, 542, 694
SchI GAGTC 2 cut(s) 266, 550
ScrFI CCNGG 2 cut(s) 592, 617
SetI ASST 7 cut(s) 33, 93, 137, 170, 415, 463, 768
SfaNI GCATC 1 cut(s) 3
SfcI CTRYAG 2 cut(s) 186, 366
Sfr274I CTCGAG 1 cut(s) 254
Sfr303I CCGCGG 1 cut(s) 281
SfuI TTCGAA 2 cut(s) 123, 240
SgrBI CCGCGG 1 cut(s) 281
SinI GGWCC 3 cut(s) 503, 542, 694
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 3 cut(s) 254, 549, 558
SmoI CTYRAG 3 cut(s) 254, 549, 558
Sse9I AATT 5 cut(s) 246, 475, 651, 745, 867
SsiI CCGC 4 cut(s) 278, 280, 483, 681
SspMI CTAG 4 cut(s) 195, 381, 480, 916
StyD4I CCNGG 2 cut(s) 590, 615
StyI CCWWGG 2 cut(s) 222, 702
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 2 cut(s) 137, 170
TaqI TCGA 6 cut(s) 11, 75, 123, 240, 255, 732
TaqII GACCGA 2 cut(s) 520, 682
TasI AATT 5 cut(s) 246, 475, 651, 745, 867
TatI WGTACW 1 cut(s) 896
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 3 cut(s) 68, 440, 722
Tru1I TTAA 1 cut(s) 866
Tru9I TTAA 1 cut(s) 866
TscAI CASTG 2 cut(s) 219, 850
TseFI GTSAC 2 cut(s) 258, 553
TseI GCWGC 7 cut(s) 24, 59, 83, 233, 365, 413, 638
Tsp45I GTSAC 2 cut(s) 258, 553
TspDTI ATGAA 3 cut(s) 144, 299, 435
TspRI CASTG 2 cut(s) 219, 850
Van91I CCANNNNNTGG 1 cut(s) 703
VpaK11BI GGWCC 3 cut(s) 503, 542, 694
VspI ATTAAT 1 cut(s) 866
XapI RAATTY 1 cut(s) 475
XhoI CTCGAG 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 4 cut(s) 195, 381, 480, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.