MD09G1170500.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004838
Physical Location & Seq
Reverse (-)
14373086 .. 14373817
732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1170500.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGCTAAGAGTCCTGATAATAGGATCACAAACAAAGATGCTGAGAATGGGAAAAAGGAGGGCAAAAGTCGAAGAAGGGTTGAAATAAAGAAAGTTGAAGACAAGAACAAGCGTCACGTGACCTTCTCCAAGCGCAAAAGGGGTCTCTTCAACAAAACAGCAGAGCTCAGCGTTTTGACCGGTGCTGAGACGGCAGCAATCGTTGTGTCAAACAGTGGCAAGGTGTTCTGCTTCGGAAGTCCCTCCCCTGACGCTGTTATCGAACGCTACGTTGGTCACAATGCCTCATTATCTGCAGGTCAACCAGATAATTATCATCCTCAAATAAACAACACTCATCGTTCTACTAATTATTTCAATGTTAATATGAGTAGTAGTAAGAAGCAAATACAAGATTATGAGAAAGCTTCGAGACACTTGAAGGAAGAGAAAATATTGAAGGGTGGGAAGAAAAGTAATAGTAACAACAATGGAGGAGATGAAGGAGGTTGTTCTTATGGGTGGTGGGAGAGACCGATTGGAATGATGAGTAGTTTGGAGGAGTTAGAAGAGTACAGGGAAGCCTTGTACAAGTTGAAGCACAATGTGGAGGTTCGAACCAATCAGCTGATTCGGGAAACTACTCCTTCCGATCACTTCTCCTCTCTTTTGTCTATGGATGATACATGGAGTCCTCCAAAATTAAGCCAGGGTCAAGGGTGCATGGGACGTGAATTTTATTATTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

244

Amino Acids

27.52

Weight (kDa)

9.34

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 31 - 78 1.4e-18 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 287
AclWI GGATC 1 cut(s) 32
AcsI RAATTY 1 cut(s) 713
AcvI CACGTG 1 cut(s) 118
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 2 cut(s) 554, 569
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 7 cut(s) 83, 98, 151, 358, 421, 439, 577
AjiI CACGTC 1 cut(s) 710
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AluBI AGCT 3 cut(s) 166, 407, 607
AluI AGCT 3 cut(s) 166, 407, 607
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 4 cut(s) 149, 182, 406, 505
AlwI GGATC 1 cut(s) 32
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 713
AsiGI ACCGGT 1 cut(s) 179
AspLEI GCGC 1 cut(s) 135
AsuII TTCGAA 1 cut(s) 595
BanII GRGCYC 1 cut(s) 168
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 1 cut(s) 689
BcoDI GTCTC 4 cut(s) 149, 182, 406, 505
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 1 cut(s) 195
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 689
BmgBI CACGTC 1 cut(s) 710
BmrFI CCNGG 1 cut(s) 689
BmsI GCATC 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 105
Bpu1102I GCTNAGC 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 312
BsaI GGTCTC 2 cut(s) 149, 505
BsaJI CCNNGG 1 cut(s) 688
BsaWI WCCGGW 1 cut(s) 179
Bse118I RCCGGY 1 cut(s) 179
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 1 cut(s) 688
BseGI GGATG 2 cut(s) 316, 664
BseJI GATNNNNATC 1 cut(s) 312
BseMII CTCAG 3 cut(s) 33, 177, 181
BseRI GAGGAG 3 cut(s) 489, 554, 631
BseXI GCAGC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 180
BslFI GGGAC 2 cut(s) 225, 720
BsmAI GTCTC 4 cut(s) 149, 182, 406, 505
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 225, 720
Bso31I GGTCTC 2 cut(s) 149, 505
Bsp119I TTCGAA 1 cut(s) 595
Bsp1286I GDGCHC 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 2 cut(s) 24, 631
Bsp1720I GCTNAGC 1 cut(s) 167
BspCNI CTCAG 3 cut(s) 34, 178, 180
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 1 cut(s) 32
BspT104I TTCGAA 1 cut(s) 595
BspTNI GGTCTC 2 cut(s) 149, 505
BsrFI RCCGGY 1 cut(s) 179
BsrGI TGTACA 1 cut(s) 567
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 688
BssMI GATC 2 cut(s) 24, 631
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 3 cut(s) 152, 420, 543
BstAUI TGTACA 1 cut(s) 567
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 595
BstDEI CTNAG 4 cut(s) 6, 42, 167, 186
BstF5I GGATG 2 cut(s) 316, 664
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 2 cut(s) 27, 634
BstMAI GTCTC 4 cut(s) 149, 182, 406, 505
BstMBI GATC 2 cut(s) 24, 631
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 687
BstSFI CTRYAG 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 105
BtrI CACGTC 1 cut(s) 710
BtsCI GGATG 2 cut(s) 316, 664
BtsIMutI CAGTG 1 cut(s) 220
BveI ACCTGC 1 cut(s) 287
CfoI GCGC 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 179
CseI GACGC 2 cut(s) 101, 260
Csp6I GTAC 2 cut(s) 553, 568
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 2 cut(s) 666, 703
CviJI RGCY 6 cut(s) 5, 166, 407, 563, 607, 687
CviKI_1 RGCY 6 cut(s) 5, 166, 407, 563, 607, 687
CviQI GTAC 2 cut(s) 553, 568
DdeI CTNAG 4 cut(s) 6, 42, 167, 186
DpnI GATC 2 cut(s) 26, 633
DpnII GATC 2 cut(s) 24, 631
DraIII CACNNNGTG 1 cut(s) 586
Eam1104I CTCTTC 3 cut(s) 152, 420, 543
EarI CTCTTC 3 cut(s) 152, 420, 543
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 2 cut(s) 149, 505
Eco53kI GAGCTC 1 cut(s) 166
Eco72I CACGTG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 687
EcoT38I GRGCYC 1 cut(s) 168
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 2 cut(s) 669, 706
FaiI YATR 6 cut(s) 368, 399, 498, 656, 667, 704
FaqI GGGAC 2 cut(s) 225, 720
FatI CATG 2 cut(s) 665, 702
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 2 cut(s) 303, 671
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 195
GlaI GCGC 1 cut(s) 134
GluI GCNGC 1 cut(s) 195
HapII CCGG 1 cut(s) 180
HgaI GACGC 2 cut(s) 101, 260
HhaI GCGC 1 cut(s) 135
Hin1II CATG 2 cut(s) 669, 706
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 1 cut(s) 302
HindII GTYRAC 1 cut(s) 302
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 3 cut(s) 10, 610, 670
HpaII CCGG 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 2 cut(s) 236, 631
Hpy188III TCNNGA 3 cut(s) 14, 411, 614
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 6 cut(s) 69, 133, 415, 433, 476, 636
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 3 cut(s) 117, 270, 709
HpyCH4V TGCA 2 cut(s) 296, 702
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 4 cut(s) 6, 42, 167, 186
HpySE526I ACGT 3 cut(s) 117, 270, 709
Hsp92II CATG 2 cut(s) 669, 706
HspAI GCGC 1 cut(s) 133
Kzo9I GATC 2 cut(s) 24, 631
LpnPI CCDG 8 cut(s) 27, 193, 261, 282, 318, 541, 674, 701
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 1 cut(s) 28
MaeII ACGT 3 cut(s) 117, 270, 709
MaeIII GTNAC 4 cut(s) 113, 118, 275, 461
MalI GATC 2 cut(s) 26, 633
MboI GATC 2 cut(s) 24, 631
MboII GAAGA 6 cut(s) 84, 110, 139, 437, 460, 560
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 4 cut(s) 310, 349, 680, 713
MlyI GAGTC 2 cut(s) 19, 679
MseI TTAA 2 cut(s) 363, 683
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 689
MvaI CCWGG 1 cut(s) 689
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 2 cut(s) 24, 631
NlaIII CATG 2 cut(s) 669, 706
NmuCI GTSAC 3 cut(s) 113, 118, 275
NspV TTCGAA 1 cut(s) 595
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 267
PfeI GAWTC 1 cut(s) 610
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 2 cut(s) 18, 678
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 2 cut(s) 18, 678
Ppu21I YACGTR 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 687
PspCI CACGTG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 687
PstI CTGCAG 1 cut(s) 298
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 2 cut(s) 554, 569
RsaNI GTAC 2 cut(s) 553, 568
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 363, 683
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 2 cut(s) 24, 631
SchI GAGTC 2 cut(s) 19, 679
ScrFI CCNGG 1 cut(s) 689
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 595
Sse9I AATT 4 cut(s) 310, 349, 680, 713
SspI AATATT 1 cut(s) 435
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 687
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 3 cut(s) 120, 273, 712
TaqI TCGA 4 cut(s) 70, 261, 410, 595
TaqII GACCGA 1 cut(s) 529
TasI AATT 4 cut(s) 310, 349, 680, 713
TatI WGTACW 2 cut(s) 552, 567
TfiI GAWTC 1 cut(s) 610
Tru1I TTAA 2 cut(s) 363, 683
Tru9I TTAA 2 cut(s) 363, 683
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 3 cut(s) 113, 118, 275
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 3 cut(s) 113, 118, 275
TspDTI ATGAA 1 cut(s) 495
TspRI CASTG 1 cut(s) 220
XapI RAATTY 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.