pycom09g09140

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004838
Physical Location & Seq
Reverse (-)
7271006 .. 7271317
312 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g09140.1

Sequence Viewer

Length: 312 bp
ATGGCTATAACTGTTGAGTATCAGATATCAAAAAAGGCTAGCAAAGGTCGAAGAAAGATTGAAATCAAGAGAGTTGAAGATAAGAACAAGCGTCACGTTACCTTCTCCAAGCGCAAAAGGCGTATCTTCAATAAAGCGGCTCAACTCAGCGTTTTGACGGGTGCAGAGGTAGCGGGGATCGTCGTGCCTAGCAACGGCAAAGTGTTCTGCTTCGGCAGCCCTGACCCTCGCACTATCATCAACCACTACCTTGCCGACAATAATGCCTCCTTATTGCTTGCAGATCAACCAGATCATCCTACTAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

104

Amino Acids

11.49

Weight (kDa)

10.2

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 24 - 71 5.1e-18 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 137, 173
AclWI GGATC 1 cut(s) 185
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 3 cut(s) 62, 77, 130
AlwI GGATC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 216
AspLEI GCGC 1 cut(s) 114
AsuNHI GCTAGC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 228
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BfaI CTAG 2 cut(s) 39, 189
BisI GCNGC 2 cut(s) 138, 217
BlsI GCNGC 2 cut(s) 139, 218
BmtI GCTAGC 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 62
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse8I GATNNNNATC 1 cut(s) 62
BseGI GGATG 1 cut(s) 295
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 1 cut(s) 160
BseXI GCAGC 1 cut(s) 228
BsgI GTGCAG 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 194
Bsp143I GATC 3 cut(s) 177, 283, 292
BspACI CCGC 2 cut(s) 137, 173
BspCNI CTCAG 1 cut(s) 159
BspOI GCTAGC 1 cut(s) 42
BspPI GGATC 1 cut(s) 185
BssMI GATC 3 cut(s) 177, 283, 292
Bst4CI ACNGT 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 40, 279
BstDEI CTNAG 1 cut(s) 146
BstF5I GGATG 1 cut(s) 295
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 3 cut(s) 180, 286, 295
BstMBI GATC 3 cut(s) 177, 283, 292
BstMWI GCNNNNNNNGC 3 cut(s) 118, 170, 216
BstV1I GCAGC 1 cut(s) 228
BtsCI GGATG 1 cut(s) 295
Cac8I GCNNGC 2 cut(s) 40, 279
CfoI GCGC 1 cut(s) 114
CseI GACGC 1 cut(s) 80
CviJI RGCY 4 cut(s) 5, 38, 140, 219
CviKI_1 RGCY 4 cut(s) 5, 38, 140, 219
DdeI CTNAG 1 cut(s) 146
DpnI GATC 3 cut(s) 179, 285, 294
DpnII GATC 3 cut(s) 177, 283, 292
Eco32I GATATC 1 cut(s) 27
EcoRV GATATC 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 310
FaiI YATR 2 cut(s) 8, 310
FauI CCCGC 1 cut(s) 166
Fnu4HI GCNGC 2 cut(s) 138, 217
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 138, 217
FspBI CTAG 2 cut(s) 39, 189
GlaI GCGC 1 cut(s) 113
GluI GCNGC 2 cut(s) 138, 217
HgaI GACGC 1 cut(s) 80
HhaI GCGC 1 cut(s) 114
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 164, 281, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 118, 170, 216
HpyF3I CTNAG 1 cut(s) 146
HpySE526I ACGT 1 cut(s) 96
HspAI GCGC 1 cut(s) 112
Kzo9I GATC 3 cut(s) 177, 283, 292
LpnPI CCDG 2 cut(s) 234, 303
Lsp1109I GCAGC 1 cut(s) 228
MaeI CTAG 2 cut(s) 39, 189
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 92, 97
MalI GATC 3 cut(s) 179, 285, 294
MboI GATC 3 cut(s) 177, 283, 292
MboII GAAGA 3 cut(s) 63, 89, 118
MnlI CCTC 3 cut(s) 160, 237, 277
Mph1103I ATGCAT 1 cut(s) 310
MwoI GCNNNNNNNGC 3 cut(s) 118, 170, 216
NdeII GATC 3 cut(s) 177, 283, 292
NheI GCTAGC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 92
NsiI ATGCAT 1 cut(s) 310
PkrI GCNGC 2 cut(s) 139, 218
SatI GCNGC 2 cut(s) 138, 217
Sau3AI GATC 3 cut(s) 177, 283, 292
SetI ASST 5 cut(s) 49, 99, 104, 171, 252
SsiI CCGC 2 cut(s) 137, 173
SspMI CTAG 2 cut(s) 39, 189
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 99
TaqI TCGA 1 cut(s) 49
TauI GCSGC 1 cut(s) 140
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 92
XspI CTAG 2 cut(s) 39, 189
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.