Rh2DG475500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004838
Physical Location & Seq
Reverse (-)
68814740 .. 68815150
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG475500.1

Sequence Viewer

Length: 411 bp
ATGAAAACAACGCTAGCTGCTGTGATCGGATGTATGATAGCTGAAACCACTGCTTCGAAGGATTCCCCCATTAGGGTTTTGGAGAAAAATATTTTGGATAACGGAAAGTTGGAATTAATGGCCCCTAATGGCAATGCCGATTATCAAAACGACAATACCGTCGTTGGATCTATAATCGTTGAAACCACGCCTTCCCGTTCAAACTTTCTCCAAAGCGACGGTGTAGTGGTCAGATCGAAGATAGTTGAAACGACTCCTTTGGGTTCGAGCTGTCCCGAAATTAGGGTTTTGGAGGACAATATAATGGGTGATGGATACTTGAATTTGATGAACGACACCGTTTTTCCTGTCAAGCCAGGCGAGTATCGCGAAAGGACTAATGGTGGATCTGAGGTGGGGGGGGGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.48

Weight (kDa)

4.6

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccII CGCG 1 cut(s) 369
AclWI GGATC 2 cut(s) 175, 394
AcsI RAATTY 1 cut(s) 322
AfiI CCNNNNNNNGG 3 cut(s) 72, 73, 282
AgsI TTSAA 4 cut(s) 182, 201, 248, 322
AjnI CCWGG 1 cut(s) 355
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 3 cut(s) 17, 41, 270
AluI AGCT 3 cut(s) 17, 41, 270
AlwI GGATC 2 cut(s) 175, 394
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 322
AseI ATTAAT 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 320
AsuII TTCGAA 1 cut(s) 56
AsuNHI GCTAGC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 4
BccI CCATC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 357
BciVI GTATCC 1 cut(s) 308
BfaI CTAG 1 cut(s) 14
BfuI GTATCC 1 cut(s) 308
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 357
BmtI GCTAGC 1 cut(s) 17
Bpu14I TTCGAA 1 cut(s) 56
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 73, 282
Bse3DI GCAATG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 3 cut(s) 72, 73, 282
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 4
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 3 cut(s) 72, 73, 282
BsmFI GGGAC 1 cut(s) 258
BsnI GGCC 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 56
Bsp143I GATC 4 cut(s) 24, 167, 233, 386
Bsp68I TCGCGA 1 cut(s) 369
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 382
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 123
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 175, 394
BspT104I TTCGAA 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 139
BssMI GATC 4 cut(s) 24, 167, 233, 386
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 3 cut(s) 160, 221, 340
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 390
BstF5I GGATG 1 cut(s) 35
BstFNI CGCG 1 cut(s) 369
BstKTI GATC 4 cut(s) 27, 170, 236, 389
BstMBI GATC 4 cut(s) 24, 167, 233, 386
BstMWI GCNNNNNNNGC 1 cut(s) 366
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 2 cut(s) 167, 386
BstYI RGATCY 2 cut(s) 167, 386
BsuI GTATCC 1 cut(s) 308
BsuRI GGCC 1 cut(s) 122
BtsCI GGATG 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
BtuMI TCGCGA 1 cut(s) 369
Cac8I GCNNGC 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 121
CviJI RGCY 5 cut(s) 17, 41, 122, 270, 355
CviKI_1 RGCY 5 cut(s) 17, 41, 122, 270, 355
DdeI CTNAG 1 cut(s) 390
DpnI GATC 4 cut(s) 26, 169, 235, 388
DpnII GATC 4 cut(s) 24, 167, 233, 386
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 355
FaiI YATR 3 cut(s) 35, 173, 302
FaqI GGGAC 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 1 cut(s) 14
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 1 cut(s) 122
HinfI GANTC 2 cut(s) 62, 253
HphI GGTGA 1 cut(s) 320
Hpy188I TCNGA 3 cut(s) 29, 233, 391
Hpy188III TCNNGA 2 cut(s) 275, 368
Hpy99I CGWCG 2 cut(s) 164, 221
HpyAV CCTTC 2 cut(s) 52, 201
HpyCH4III ACNGT 3 cut(s) 160, 221, 340
HpyF10VI GCNNNNNNNGC 1 cut(s) 366
HpyF3I CTNAG 1 cut(s) 390
Kzo9I GATC 4 cut(s) 24, 167, 233, 386
LpnPI CCDG 3 cut(s) 342, 360, 369
Lsp1109I GCAGC 1 cut(s) 4
MaeI CTAG 1 cut(s) 14
MalI GATC 4 cut(s) 26, 169, 235, 388
MboI GATC 4 cut(s) 24, 167, 233, 386
MboII GAAGA 1 cut(s) 250
MflI RGATCY 2 cut(s) 167, 386
MluCI AATT 3 cut(s) 113, 279, 322
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 2 cut(s) 90, 145
MnlI CCTC 2 cut(s) 286, 385
MseI TTAA 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 366
NdeII GATC 4 cut(s) 24, 167, 233, 386
NheI GCTAGC 1 cut(s) 13
NlaIV GGNNCC 1 cut(s) 123
NruI TCGCGA 1 cut(s) 369
NspV TTCGAA 1 cut(s) 56
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 121
PsuI RGATCY 2 cut(s) 167, 386
RruI TCGCGA 1 cut(s) 369
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 4 cut(s) 24, 167, 233, 386
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 357
SetI ASST 4 cut(s) 19, 43, 272, 396
SfuI TTCGAA 1 cut(s) 56
Sse9I AATT 3 cut(s) 113, 279, 322
SspI AATATT 1 cut(s) 91
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 355
TaaI ACNGT 3 cut(s) 160, 221, 340
TaqI TCGA 3 cut(s) 56, 236, 266
TasI AATT 3 cut(s) 113, 279, 322
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 2 cut(s) 17, 344
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 1 cut(s) 55
VspI ATTAAT 1 cut(s) 116
XapI RAATTY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.