MD09G1176200.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004838
Physical Location & Seq
Forward (+)
15072269 .. 15072988
720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1176200.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGTTAAGAGTCCTGATAATAAGATCACAGACATAGGTACTACTAATGGGAAAAAGGAGAGCAAAGGTCGAAGAAGGGTTGAAATCAAGAGAGTTGAAGACAAGAACAAGCGTCACGTGACCTTCTCCAAGCGCAAAAGGGGTCTCTTCAACAAAGCCGCGGAGCTCAGCGTTTTTACTGGTGCAGAGACGGTTGCCATTGTCGTGTCAAGCAATGGCAAGGTTTTCTGCTTCGGAAGCCCCTCACCTGACACCGTCATCAAGCGCTACCTTAGTGACAATACCTTCTTACTACCTGCAGGTCAACCAGATAATCATCTCCAAAACAACAACATTCACCATTCGAGTAATAAGAAACAAATAAAGGATTATGTGGAAGCTTCGACACGCTTAAAGGAAGCGAAAGTATTGAAGGGTGGGAAGAAAAATTATAGTAATAATAATGGAGGAGATGGAGGAGATGGAGGAGATGGAGGTTGTTCTTATGGGTGGTGGGAGAGGCCAATTGGGATGATGACTAGTTTGGAAGAGTTGGAAGAGTACAGGGATGCTTTGTACAAATTGAAACACAATGTGGAGGTTCGAACGAATCAGATGATCCGGGAAACTAGTCCTTCCACTCACTTTTCCCCGCTTTTGTTTATGGATGATATCTGGAATTCTCCTGAATTAAGCCATGGTCAAGGGTGCATGGCACCAGAATTTAATTTCTTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

240

Amino Acids

26.83

Weight (kDa)

9.22

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 31 - 78 2.7e-18 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 290, 304
AccB1I GGYRCC 1 cut(s) 694
AccII CGCG 1 cut(s) 161
AciI CCGC 3 cut(s) 159, 161, 632
AclWI GGATC 1 cut(s) 592
AcsI RAATTY 2 cut(s) 658, 701
AcvI CACGTG 1 cut(s) 118
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 3 cut(s) 40, 542, 557
AfeI AGCGCT 1 cut(s) 266
AgsI TTSAA 5 cut(s) 83, 98, 151, 412, 565
AhlI ACTAGT 2 cut(s) 518, 608
AluBI AGCT 2 cut(s) 166, 380
AluI AGCT 2 cut(s) 166, 380
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 2 cut(s) 149, 182
AlwI GGATC 1 cut(s) 592
Aor51HI AGCGCT 1 cut(s) 266
AoxI GGCC 1 cut(s) 500
ApoI RAATTY 2 cut(s) 658, 701
AspLEI GCGC 2 cut(s) 135, 267
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 2 cut(s) 237, 329
AsuII TTCGAA 1 cut(s) 583
BanI GGYRCC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 168
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 3 cut(s) 446, 455, 464
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 2 cut(s) 149, 182
BcuI ACTAGT 2 cut(s) 518, 608
BfaI CTAG 2 cut(s) 519, 609
BfmI CTRYAG 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 2 cut(s) 290, 304
BisI GCNGC 1 cut(s) 159
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 602
BmiI GGNNCC 1 cut(s) 696
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 538
BpiI GAAGAC 1 cut(s) 105
Bpu1102I GCTNAGC 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 602
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 159, 676
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 159, 676
BseGI GGATG 3 cut(s) 516, 553, 652
BseJI GATNNNNATC 1 cut(s) 315
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 3 cut(s) 462, 471, 480
BsgI GTGCAG 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 1 cut(s) 502
BshNI GGYRCC 1 cut(s) 694
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 601
BsmAI GTCTC 2 cut(s) 149, 182
BsmBI CGTCTC 1 cut(s) 182
BsnI GGCC 1 cut(s) 502
Bso31I GGTCTC 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 583
Bsp1286I GDGCHC 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 2 cut(s) 24, 597
Bsp1720I GCTNAGC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 3 cut(s) 159, 161, 632
BspANI GGCC 1 cut(s) 502
BspCNI CTCAG 1 cut(s) 180
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 1 cut(s) 696
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 2 cut(s) 290, 304
BspPI GGATC 1 cut(s) 592
BspT104I TTCGAA 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 694
BspTNI GGTCTC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 220
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 159, 676
BssMI GATC 2 cut(s) 24, 597
BssT1I CCWWGG 1 cut(s) 676
Bst4CI ACNGT 2 cut(s) 193, 256
Bst6I CTCTTC 3 cut(s) 152, 522, 531
BstAUI TGTACA 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 583
BstDEI CTNAG 2 cut(s) 167, 272
BstDSI CCRYGG 2 cut(s) 159, 676
BstF5I GGATG 3 cut(s) 516, 553, 652
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 135, 267
BstKTI GATC 2 cut(s) 27, 600
BstMAI GTCTC 2 cut(s) 149, 182
BstMBI GATC 2 cut(s) 24, 597
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 1 cut(s) 161
BstV2I GAAGAC 1 cut(s) 105
BsuRI GGCC 1 cut(s) 502
BtgI CCRYGG 2 cut(s) 159, 676
BtsCI GGATG 3 cut(s) 516, 553, 652
BveI ACCTGC 2 cut(s) 290, 304
CfoI GCGC 2 cut(s) 135, 267
Cfr42I CCGCGG 1 cut(s) 162
CseI GACGC 1 cut(s) 101
Csp6I GTAC 3 cut(s) 39, 541, 556
CviAII CATG 2 cut(s) 677, 691
CviJI RGCY 6 cut(s) 158, 166, 240, 380, 502, 675
CviKI_1 RGCY 6 cut(s) 158, 166, 240, 380, 502, 675
CviQI GTAC 3 cut(s) 39, 541, 556
DdeI CTNAG 2 cut(s) 167, 272
DpnI GATC 2 cut(s) 26, 599
DpnII GATC 2 cut(s) 24, 597
DraIII CACNNNGTG 1 cut(s) 574
Eam1104I CTCTTC 3 cut(s) 152, 522, 531
EarI CTCTTC 3 cut(s) 152, 522, 531
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 676
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 652
Eco47III AGCGCT 1 cut(s) 266
Eco53kI GAGCTC 1 cut(s) 166
Eco72I CACGTG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 658
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 676
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 2 cut(s) 680, 694
FaiI YATR 9 cut(s) 35, 372, 432, 486, 644, 678, 692, 716, 718
FatI CATG 2 cut(s) 676, 690
FauI CCCGC 1 cut(s) 639
FauNDI CATATG 1 cut(s) 716
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 3 cut(s) 523, 560, 659
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 2 cut(s) 519, 609
GlaI GCGC 2 cut(s) 134, 266
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 1 cut(s) 502
HapII CCGG 1 cut(s) 601
HgaI GACGC 1 cut(s) 101
HhaI GCGC 2 cut(s) 135, 267
Hin1II CATG 2 cut(s) 680, 694
Hin6I GCGC 2 cut(s) 133, 265
HinP1I GCGC 2 cut(s) 133, 265
HincII GTYRAC 1 cut(s) 305
HindII GTYRAC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 2 cut(s) 10, 589
HpaII CCGG 1 cut(s) 601
HphI GGTGA 2 cut(s) 237, 329
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 2 cut(s) 236, 594
Hpy188III TCNNGA 4 cut(s) 14, 88, 655, 665
Hpy8I GTNNAC 1 cut(s) 305
HpyAV CCTTC 5 cut(s) 69, 133, 295, 406, 624
HpyCH4III ACNGT 2 cut(s) 193, 256
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 3 cut(s) 185, 299, 690
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 167, 272
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 2 cut(s) 680, 694
HspAI GCGC 2 cut(s) 133, 265
KspI CCGCGG 1 cut(s) 162
Kzo9I GATC 2 cut(s) 24, 597
LmnI GCTCC 1 cut(s) 163
LweI GCATC 1 cut(s) 538
MaeI CTAG 2 cut(s) 519, 609
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 113, 118, 275
MalI GATC 2 cut(s) 26, 599
MboI GATC 2 cut(s) 24, 597
MboII GAAGA 7 cut(s) 84, 110, 139, 433, 539, 548, 703
MfeI CAATTG 1 cut(s) 504
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 7 cut(s) 427, 504, 560, 658, 668, 701, 706
MlyI GAGTC 1 cut(s) 19
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 7 cut(s) 253, 440, 449, 458, 467, 492, 571
MseI TTAA 4 cut(s) 6, 392, 671, 705
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 601
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 1 cut(s) 504
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 237
NciI CCSGG 1 cut(s) 602
NcoI CCATGG 1 cut(s) 676
NdeI CATATG 1 cut(s) 716
NdeII GATC 2 cut(s) 24, 597
NlaIII CATG 2 cut(s) 680, 694
NlaIV GGNNCC 1 cut(s) 696
NmuCI GTSAC 3 cut(s) 113, 118, 275
NspV TTCGAA 1 cut(s) 583
PfeI GAWTC 1 cut(s) 589
PflFI GACNNNGTC 1 cut(s) 254
PfoI TCCNGGA 1 cut(s) 600
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 18
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
PpsI GAGTC 1 cut(s) 18
Ppu21I YACGTR 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 168
PspCI CACGTG 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 696
PstI CTGCAG 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 3 cut(s) 40, 542, 557
RsaNI GTAC 3 cut(s) 39, 541, 556
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 1 cut(s) 168
SacII CCGCGG 1 cut(s) 162
SaqAI TTAA 4 cut(s) 6, 392, 671, 705
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 24, 597
SbfI CCTGCAGG 1 cut(s) 301
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 602
SdaI CCTGCAGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 297
Sfr303I CCGCGG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 583
SgrBI CCGCGG 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 203
SpeI ACTAGT 2 cut(s) 518, 608
Sse8387I CCTGCAGG 1 cut(s) 301
Sse9I AATT 7 cut(s) 427, 504, 560, 658, 668, 701, 706
SsiI CCGC 3 cut(s) 159, 161, 632
SspMI CTAG 2 cut(s) 519, 609
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 2 cut(s) 193, 256
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 70, 344, 383, 583
TasI AATT 7 cut(s) 427, 504, 560, 658, 668, 701, 706
TatI WGTACW 2 cut(s) 540, 555
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 4 cut(s) 6, 392, 671, 705
Tru9I TTAA 4 cut(s) 6, 392, 671, 705
TseFI GTSAC 3 cut(s) 113, 118, 275
Tsp45I GTSAC 3 cut(s) 113, 118, 275
TspDTI ATGAA 1 cut(s) 703
Tth111I GACNNNGTC 1 cut(s) 254
XapI RAATTY 2 cut(s) 658, 701
XspI CTAG 2 cut(s) 519, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.