MD17G1150800.v1.1
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004838
Physical Location & Seq
Reverse (-)
13885200 .. 13885949
750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1150800.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCTAAGGGTCCTGATAATAAGATCACAGACAAAGATATTGCTAATGGGAAAAAGGAGAGGGAATGTCGAAGAAGGGTTGAAATCAAGAGAGTTGAAGACAAGAACAAGCGTCACGTGACCTTCTCCAAGCGCAAAAGGGGTATTTTCAACAAAGCCGCGGAACTCAGCGTTTTGACTGGCGCAGAGACGGCTGCAATTGTCGTGTCAAGCAATGGCAAGGTTTTCTGCTTCGGAAGCCCCTCCCCTGACGCGGTCATTGACCTCTACCTTGGCGACAATACCTCCTTACTACCGGAAGGTCAACCAGATAATCATCTCCAAAACAACAACATTCACCATTCTAGTAGTAAAAAACAAATAGAGGATTATGTGAAAGCTTCGAGACGCTTGAAGGAAGCGAAAATACTGAAGGGTGGGAAGAATAGTAATAATAATGGAGGAGAAGGAGGGGAAACAGGGTGTTCTTATGGGTGGTGGGAGAGGTGGATTGGGATGATGAGTAGTTTGGAAGAATTAGAAGAGTACAGGGATGCCCTGTACAAGTTGAAACACAACGTGGAGGTTCGAACTAATCAGATGATTCGGGAAACTAGTCCTTCCAATCACTTTTCCCCACTTTTGTTTATGGATGATATCTGGAGTTCTCCTGAATTAAGCCAGGGTCAAGGGTGGCCCCGGAATTTAATTTCTTCATATGATATCGATGTAAAGAAGTATGATTGGAACGGAGTTACCTATCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04111 map04011 map04111
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

250

Amino Acids

28.25

Weight (kDa)

8.8

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 31 - 78 8.4e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 161, 254
AciI CCGC 3 cut(s) 159, 161, 254
AcsI RAATTY 1 cut(s) 682
AcuI CTGAAG 1 cut(s) 431
AcvI CACGTG 1 cut(s) 118
AdeI CACNNNGTG 1 cut(s) 559
AfaI GTAC 2 cut(s) 527, 542
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 5 cut(s) 83, 98, 151, 394, 550
AhlI ACTAGT 1 cut(s) 593
AjnI CCWGG 1 cut(s) 660
AluBI AGCT 1 cut(s) 380
AluI AGCT 1 cut(s) 380
Alw26I GTCTC 2 cut(s) 182, 379
AoxI GGCC 1 cut(s) 674
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 682
AspLEI GCGC 2 cut(s) 135, 185
AspS9I GGNCC 2 cut(s) 11, 675
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 568
AvaII GGWCC 1 cut(s) 11
BarI GAAGNNNNNNTAC 2 cut(s) 390, 422
BbrPI CACGTG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 1 cut(s) 662
BcnI CCSGG 1 cut(s) 679
BcoDI GTCTC 2 cut(s) 182, 379
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 2 cut(s) 345, 594
BisI GCNGC 2 cut(s) 159, 195
BlsI GCNGC 2 cut(s) 160, 196
Bme1390I CCNGG 2 cut(s) 662, 679
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 2 cut(s) 11, 675
BmiI GGNNCC 2 cut(s) 12, 677
BmrFI CCNGG 2 cut(s) 662, 679
BmsI GCATC 1 cut(s) 523
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 661
Bpu10I CCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 568
BpuMI CCSGG 1 cut(s) 679
Bsa29I ATCGAT 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 315
BsaJI CCNNGG 4 cut(s) 159, 271, 661, 677
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 662
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 4 cut(s) 159, 271, 661, 677
BseGI GGATG 3 cut(s) 501, 538, 637
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 253
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 181
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 456
BseXI GCAGC 1 cut(s) 181
Bsh1236I CGCG 2 cut(s) 161, 254
BshFI GGCC 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 705
BsiSI CCGG 2 cut(s) 296, 679
BslI CCNNNNNNNGG 1 cut(s) 253
BsmAI GTCTC 2 cut(s) 182, 379
BsmBI CGTCTC 2 cut(s) 182, 379
BsnI GGCC 1 cut(s) 676
Bsp119I TTCGAA 1 cut(s) 568
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 1 cut(s) 24
BspACI CCGC 3 cut(s) 159, 161, 254
BspANI GGCC 1 cut(s) 676
BspCNI CTCAG 1 cut(s) 180
BspDI ATCGAT 1 cut(s) 705
BspFNI CGCG 2 cut(s) 161, 254
BspLI GGNNCC 2 cut(s) 12, 677
BspT104I TTCGAA 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 220
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 4 cut(s) 159, 271, 661, 677
BssMI GATC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 662
Bst6I CTCTTC 1 cut(s) 516
BstAUI TGTACA 1 cut(s) 540
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 568
BstDEI CTNAG 2 cut(s) 6, 167
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 3 cut(s) 501, 538, 637
BstFNI CGCG 2 cut(s) 161, 254
BstHHI GCGC 2 cut(s) 135, 185
BstKTI GATC 1 cut(s) 27
BstMAI GTCTC 2 cut(s) 182, 379
BstMBI GATC 1 cut(s) 24
BstMWI GCNNNNNNNGC 2 cut(s) 191, 237
BstNI CCWGG 1 cut(s) 662
BstSCI CCNGG 2 cut(s) 660, 677
BstUI CGCG 2 cut(s) 161, 254
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 1 cut(s) 676
BsuTUI ATCGAT 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 159
BtsCI GGATG 3 cut(s) 501, 538, 637
CfoI GCGC 2 cut(s) 135, 185
Cfr13I GGNCC 2 cut(s) 11, 675
Cfr42I CCGCGG 1 cut(s) 162
ClaI ATCGAT 1 cut(s) 705
CseI GACGC 3 cut(s) 101, 260, 396
Csp6I GTAC 2 cut(s) 526, 541
CviJI RGCY 7 cut(s) 5, 158, 194, 240, 380, 660, 676
CviKI_1 RGCY 7 cut(s) 5, 158, 194, 240, 380, 660, 676
CviQI GTAC 2 cut(s) 526, 541
DdeI CTNAG 2 cut(s) 6, 167
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
DraIII CACNNNGTG 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 271
Eco32I GATATC 2 cut(s) 637, 703
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 431
Eco72I CACGTG 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 660
EcoRV GATATC 2 cut(s) 637, 703
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 2 cut(s) 182, 379
FaiI YATR 6 cut(s) 372, 471, 629, 697, 699, 720
FauNDI CATATG 1 cut(s) 697
Fnu4HI GCNGC 2 cut(s) 159, 195
FokI GGATG 3 cut(s) 508, 545, 644
Fsp4HI GCNGC 2 cut(s) 159, 195
FspBI CTAG 2 cut(s) 345, 594
GlaI GCGC 2 cut(s) 134, 184
GluI GCNGC 2 cut(s) 159, 195
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 1 cut(s) 676
HapII CCGG 2 cut(s) 296, 679
HgaI GACGC 3 cut(s) 101, 260, 396
HhaI GCGC 2 cut(s) 135, 185
Hin6I GCGC 2 cut(s) 133, 183
HinP1I GCGC 2 cut(s) 133, 183
HincII GTYRAC 1 cut(s) 305
HindII GTYRAC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 1 cut(s) 583
HpaII CCGG 2 cut(s) 296, 679
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 2 cut(s) 236, 579
Hpy188III TCNNGA 6 cut(s) 14, 88, 384, 587, 640, 650
Hpy8I GTNNAC 1 cut(s) 305
HpyAV CCTTC 7 cut(s) 69, 133, 293, 388, 406, 440, 609
HpyCH4IV ACGT 2 cut(s) 117, 558
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 237
HpyF3I CTNAG 2 cut(s) 6, 167
HpySE526I ACGT 2 cut(s) 117, 558
HspAI GCGC 2 cut(s) 133, 183
KspI CCGCGG 1 cut(s) 162
Kzo9I GATC 1 cut(s) 24
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 523
MaeI CTAG 2 cut(s) 345, 594
MaeII ACGT 2 cut(s) 117, 558
MaeIII GTNAC 3 cut(s) 113, 118, 733
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 6 cut(s) 84, 110, 433, 524, 533, 684
MfeI CAATTG 1 cut(s) 198
MluCI AATT 5 cut(s) 198, 515, 653, 682, 687
MnlI CCTC 9 cut(s) 54, 253, 275, 295, 358, 434, 443, 477, 556
MseI TTAA 2 cut(s) 656, 686
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 296, 679
MspR9I CCNGG 2 cut(s) 662, 679
MunI CAATTG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 2 cut(s) 161, 254
MwoI GCNNNNNNNGC 2 cut(s) 191, 237
NciI CCSGG 1 cut(s) 679
NdeI CATATG 1 cut(s) 697
NdeII GATC 1 cut(s) 24
NlaIV GGNNCC 2 cut(s) 12, 677
NmuCI GTSAC 2 cut(s) 113, 118
NspV TTCGAA 1 cut(s) 568
PfeI GAWTC 1 cut(s) 583
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 160, 196
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 660
PspCI CACGTG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 2 cut(s) 12, 677
PspPI GGNCC 2 cut(s) 11, 675
PspPPI RGGWCCY 1 cut(s) 11
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 2 cut(s) 527, 542
RsaNI GTAC 2 cut(s) 526, 541
SacII CCGCGG 1 cut(s) 162
SaqAI TTAA 2 cut(s) 656, 686
SatI GCNGC 2 cut(s) 159, 195
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 2 cut(s) 11, 675
ScrFI CCNGG 2 cut(s) 662, 679
SfaNI GCATC 1 cut(s) 523
Sfr303I CCGCGG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 568
SgrBI CCGCGG 1 cut(s) 162
SinI GGWCC 1 cut(s) 11
SpeI ACTAGT 1 cut(s) 593
Sse9I AATT 5 cut(s) 198, 515, 653, 682, 687
SsiI CCGC 3 cut(s) 159, 161, 254
SspMI CTAG 2 cut(s) 345, 594
StyD4I CCNGG 2 cut(s) 660, 677
StyI CCWWGG 1 cut(s) 271
TaiI ACGT 2 cut(s) 120, 561
TaqI TCGA 4 cut(s) 70, 383, 568, 705
TasI AATT 5 cut(s) 198, 515, 653, 682, 687
TatI WGTACW 2 cut(s) 525, 540
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 2 cut(s) 656, 686
Tru9I TTAA 2 cut(s) 656, 686
TseFI GTSAC 2 cut(s) 113, 118
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 2 cut(s) 113, 118
TspDTI ATGAA 1 cut(s) 684
TspGWI ACGGA 1 cut(s) 744
Tth111I GACNNNGTC 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 682
XspI CTAG 2 cut(s) 345, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.