MD10G1139500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000902
Physical Location & Seq
Forward (+)
22433937 .. 22436133
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1139500.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGGAACTACTGAATCTGAGTCGCCTATTCATGATAAACAACTGCCAGTTCCTGTAAAGAAGACAGTGTTAAGAGATCTGCAAAATGTGCCCAAGGATATAGGACGCACTAGTAATGTCCCTAGAGTTTCGAGCACCAAGAGACCTTTACCTGAGCGGCAGATGAGCCCTCCTCAACACCAATCCCACGGTAGCAGTGCTGCAAATGCACAGCTTGTCTATGTTCGTAGAAAATCAGAAGCAGACGCAGTCAGAAATAACGCATGTGATAAAGCAACCATTAATGCCGAGTGTCAGGTGTCAAGGAAACTTAGTCACCACGACGCAAGCACCCGACCCATATCCCAAGCAAAGGAAACAAAAGTTTTTTGTGTACCGGCTTTTGCACCTTTTCCAGTGGCAGCTTCAACCATTCTGCCTGGGAAACCTTCAGTTCTGGTCCCGCTTTGTAAGTCTGGTACTGGACTACAACCAGCAGGAGCCAATTGCCATCCCCATACTTCTGCGACATTGCCAAAGGGTCTAAAAAATCTGCACTGGGAAGGGCGATTCCATCAATTGCAGTTGCTGTTGAGGAAATTGGACCAATCAGAGCATGAGGATTATATCCAGATGCTACGGTCGCTGTCCTCTGTTGAACTGAGCAGACTTGCAGTTGAGTTGGAAAAGAGATCAATTCAACTTTCACTGGAGGAAGCAAAAGAGTTGCAACGTGTTGGGTGTTTCAATGTTCTTGGGAAATCTGTGAAGCACTTTAATGTGCCCTCAACTCAGGAAGACCGATCCGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN52

Protein Analysis

265

Amino Acids

29.18

Weight (kDa)

9.51

Isoelectric Point (pI)

66.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 157
AciI CCGC 2 cut(s) 157, 443
AclWI GGATC 1 cut(s) 777
AcuI CTGAAG 1 cut(s) 414
AfaI GTAC 2 cut(s) 375, 460
AfiI CCNNNNNNNGG 1 cut(s) 352
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 408, 638, 680, 727
AhlI ACTAGT 1 cut(s) 110
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 2 cut(s) 214, 404
AluI AGCT 2 cut(s) 214, 404
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 1 cut(s) 777
AlwNI CAGNNNCTG 2 cut(s) 53, 568
ApeKI GCWGC 2 cut(s) 200, 401
AseI ATTAAT 1 cut(s) 282
AspS9I GGNCC 2 cut(s) 439, 583
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 2 cut(s) 439, 583
BaeGI GKGCMC 2 cut(s) 93, 765
BanII GRGCYC 1 cut(s) 170
BbsI GAAGAC 2 cut(s) 68, 783
Bbv12I GWGCWC 1 cut(s) 137
BbvI GCAGC 2 cut(s) 187, 413
BccI CCATC 2 cut(s) 498, 561
BciT130I CCWGG 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 136
BcuI ACTAGT 1 cut(s) 110
BfaI CTAG 2 cut(s) 111, 123
BglII AGATCT 1 cut(s) 76
BisI GCNGC 3 cut(s) 158, 201, 402
BlsI GCNGC 3 cut(s) 159, 202, 403
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 2 cut(s) 439, 583
BmgT120I GGNCC 2 cut(s) 439, 583
BmiI GGNNCC 2 cut(s) 441, 481
BmrFI CCNGG 1 cut(s) 420
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 1 cut(s) 603
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 2 cut(s) 68, 783
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 1 cut(s) 710
Bpu10I CCTNAGC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 3 cut(s) 93, 187, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 5 cut(s) 47, 395, 466, 542, 693
Bse3DI GCAATG 1 cut(s) 509
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 3 cut(s) 93, 187, 419
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 4 cut(s) 9, 144, 632, 785
BseNI ACTGG 5 cut(s) 47, 395, 466, 542, 693
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 2 cut(s) 93, 765
BseXI GCAGC 2 cut(s) 187, 413
BsgI GTGCAG 1 cut(s) 518
Bsh1285I CGRYCG 1 cut(s) 623
BsiEI CGRYCG 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 377
BslFI GGGAC 2 cut(s) 104, 425
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 136
BsmFI GGGAC 2 cut(s) 104, 425
Bso31I GGTCTC 1 cut(s) 136
Bsp1286I GDGCHC 4 cut(s) 93, 137, 170, 765
Bsp143I GATC 3 cut(s) 76, 671, 782
BspACI CCGC 2 cut(s) 157, 443
BspCNI CTCAG 4 cut(s) 10, 145, 633, 784
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 441, 481
BspPI GGATC 1 cut(s) 777
BspTNI GGTCTC 1 cut(s) 136
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 376
BsrI ACTGG 5 cut(s) 47, 395, 466, 542, 693
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 3 cut(s) 93, 187, 419
BssMI GATC 3 cut(s) 76, 671, 782
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 67, 191, 621
BstAPI GCANNNNNTGC 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 5 cut(s) 18, 153, 311, 641, 771
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 1 cut(s) 490
BstKTI GATC 3 cut(s) 79, 674, 785
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 3 cut(s) 76, 671, 782
BstMCI CGRYCG 1 cut(s) 623
BstMWI GCNNNNNNNGC 3 cut(s) 88, 206, 622
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 418
BstSLI GKGCMC 2 cut(s) 93, 765
BstV1I GCAGC 2 cut(s) 187, 413
BstV2I GAAGAC 2 cut(s) 68, 783
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 1 cut(s) 490
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 5 cut(s) 72, 202, 402, 535, 686
Cac8I GCNNGC 1 cut(s) 328
CaiI CAGNNNCTG 2 cut(s) 53, 568
CciI TCATGA 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 2 cut(s) 439, 583
CseI GACGC 3 cut(s) 114, 254, 332
Csp6I GTAC 2 cut(s) 374, 459
CviAII CATG 3 cut(s) 32, 264, 596
CviJI RGCY 5 cut(s) 168, 214, 380, 404, 482
CviKI_1 RGCY 5 cut(s) 168, 214, 380, 404, 482
CviQI GTAC 2 cut(s) 374, 459
DdeI CTNAG 5 cut(s) 18, 153, 311, 641, 771
DpnI GATC 3 cut(s) 78, 673, 784
DpnII GATC 3 cut(s) 76, 671, 782
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 2 cut(s) 439, 583
Eco57I CTGAAG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 3 cut(s) 35, 267, 599
FaiI YATR 8 cut(s) 33, 101, 222, 265, 341, 498, 597, 606
FaqI GGGAC 2 cut(s) 104, 425
FatI CATG 3 cut(s) 31, 263, 595
FauI CCCGC 1 cut(s) 450
Fnu4HI GCNGC 3 cut(s) 158, 201, 402
FokI GGATG 1 cut(s) 477
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 3 cut(s) 158, 201, 402
FspBI CTAG 2 cut(s) 111, 123
GluI GCNGC 3 cut(s) 158, 201, 402
GsuI CTGGAG 1 cut(s) 710
HapII CCGG 1 cut(s) 377
HgaI GACGC 3 cut(s) 114, 254, 332
Hin1II CATG 3 cut(s) 35, 267, 599
HinfI GANTC 3 cut(s) 14, 20, 549
HpaII CCGG 1 cut(s) 377
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 374
Hpy188I TCNGA 5 cut(s) 19, 238, 254, 592, 787
Hpy188III TCNNGA 3 cut(s) 32, 610, 773
Hpy8I GTNNAC 1 cut(s) 374
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 2 cut(s) 438, 536
HpyCH4III ACNGT 3 cut(s) 67, 191, 621
HpyCH4IV ACGT 1 cut(s) 712
HpyCH4V TGCA 8 cut(s) 82, 203, 209, 386, 535, 562, 653, 709
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 206, 622
HpyF3I CTNAG 5 cut(s) 18, 153, 311, 641, 771
HpySE526I ACGT 1 cut(s) 712
Hsp92II CATG 3 cut(s) 35, 267, 599
Kzo9I GATC 3 cut(s) 76, 671, 782
LmnI GCTCC 1 cut(s) 479
Lsp1109I GCAGC 2 cut(s) 187, 413
LweI GCATC 1 cut(s) 603
MaeI CTAG 2 cut(s) 111, 123
MaeII ACGT 1 cut(s) 712
MaeIII GTNAC 1 cut(s) 314
MalI GATC 3 cut(s) 78, 673, 784
MbiI CCGCTC 1 cut(s) 157
MboI GATC 3 cut(s) 76, 671, 782
MboII GAAGA 2 cut(s) 73, 788
MfeI CAATTG 2 cut(s) 484, 557
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 4 cut(s) 93, 137, 170, 765
MluCI AATT 4 cut(s) 484, 557, 578, 675
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 642
MnlI CCTC 7 cut(s) 180, 183, 567, 592, 640, 685, 775
MseI TTAA 3 cut(s) 71, 282, 756
MslI CAYNNNNRTG 1 cut(s) 756
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 420
MunI CAATTG 2 cut(s) 484, 557
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 88, 206, 622
NdeII GATC 3 cut(s) 76, 671, 782
NlaIII CATG 3 cut(s) 35, 267, 599
NlaIV GGNNCC 2 cut(s) 441, 481
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 314
NspI RCATGY 1 cut(s) 267
PagI TCATGA 1 cut(s) 31
PfeI GAWTC 2 cut(s) 14, 549
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 3 cut(s) 159, 202, 403
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PshBI ATTAAT 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 441, 481
PspPI GGNCC 2 cut(s) 439, 583
PstNI CAGNNNCTG 2 cut(s) 53, 568
PsuI RGATCY 1 cut(s) 76
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 2 cut(s) 375, 460
RsaNI GTAC 2 cut(s) 374, 459
RseI CAYNNNNRTG 1 cut(s) 756
SaqAI TTAA 3 cut(s) 71, 282, 756
SatI GCNGC 3 cut(s) 158, 201, 402
Sau3AI GATC 3 cut(s) 76, 671, 782
Sau96I GGNCC 2 cut(s) 439, 583
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 4 cut(s) 93, 137, 170, 765
SetI ASST 8 cut(s) 148, 154, 216, 300, 391, 406, 430, 715
SfaNI GCATC 1 cut(s) 603
SinI GGWCC 2 cut(s) 439, 583
SmiMI CAYNNNNRTG 1 cut(s) 756
SpeI ACTAGT 1 cut(s) 110
Sse9I AATT 4 cut(s) 484, 557, 578, 675
SsiI CCGC 2 cut(s) 157, 443
SspMI CTAG 2 cut(s) 111, 123
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 67, 191, 621
TaiI ACGT 1 cut(s) 715
TaqI TCGA 1 cut(s) 131
TaqII GACCGA 1 cut(s) 795
TasI AATT 4 cut(s) 484, 557, 578, 675
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 2 cut(s) 14, 549
Tru1I TTAA 3 cut(s) 71, 282, 756
Tru9I TTAA 3 cut(s) 71, 282, 756
TscAI CASTG 5 cut(s) 72, 202, 402, 542, 693
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 200, 401
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 5 cut(s) 72, 202, 402, 542, 693
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 439, 583
VspI ATTAAT 1 cut(s) 282
XceI RCATGY 1 cut(s) 267
XspI CTAG 2 cut(s) 111, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.