pycom05g13100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000902
Physical Location & Seq
Forward (+)
16673482 .. 16677497
4016 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13100.6

Sequence Viewer

Length: 984 bp
ATGACAATCAATAAACAGAACGGGGAGACCAAACTACAAATTCGTCGCGTTATTCGAGAGCCAAAGCTTTTGGTTTTGAAACCCCTTCTTCCTTCATATGTTTCAAGCGGTTCCAATGAGCTAAGGGTCTGTCAATTACTTCAGATGGTTCAGCAAACCATAGATTCTATAGTCAGTGAATATGGGATGGGAACTACAGAATCTGAGTCGTCTATTCATGATAAACGACAGCCAGTTCCTGTAAAGAAGACAGTGTTAAGAGATCTGCAAAATGTTCCCAAGGATAGACCCACTAGTAATGCCCCTAGAGTTTCGGGTACCAAGAGACCTTTACCTGAGAGGCCAATGAGCCCTCCTCAACACCAATCCCCCAGTAGCAGTCCTGCAAATACGCAGCTTGTCTATGTTCGTAGAAAATCTGAAGCAGATGCAGTCAGAAATAGTACATGTGATAATGCAAGCATTAATGCTGAGTGTCAGATGTCAAGGAAACTTAGTCACCATGAGGCAAGCACCCAACCCATACTCCAAACAAAGGAAACAAAAGTTTATTGTGTGCCGGCTTTTGCACCTTTTCCAGTGGCAGCTTCAACCATTCCGCCTGGAAAACCTTCAGTTCCGGTCCCGCCTAGTAAGTCTGGTACTGGACTACCACCAGCAGGAGCCAATGGTCATCCCCATACTTCTTCTACAGTGCATTGTTCAATGGGTCTAAAAAATCTGCACTGGGAAGAGCGATTCCATCAATTGCAGTTGCTATTGAGGAACTTGGACCAATCAGAACATGAGGATTATATCCAGATGCTGCGGTCGCTCTCCTCGGTTGAACTGAGCAGACTTGCAGTTGAGTTGGAAAAGAGATCAATTCAGCTTTCACTGGAGGAAGCAAAAGAGTTGCAGCACGTTGGGCGATTGAATGTTCTGGGGAAATCTGTGAAGCAGTTTAAAGTGCCCTCAACTCAGGAAGACCGATCGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN52

Protein Analysis

328

Amino Acids

36.34

Weight (kDa)

9.33

Isoelectric Point (pI)

70.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 1 cut(s) 317
AccII CGCG 1 cut(s) 48
AciI CCGC 4 cut(s) 108, 599, 626, 808
AcsI RAATTY 1 cut(s) 39
AcuI CTGAAG 3 cut(s) 125, 441, 597
AfaI GTAC 3 cut(s) 319, 445, 643
AfiI CCNNNNNNNGG 3 cut(s) 535, 659, 976
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 6 cut(s) 79, 105, 591, 705, 827, 916
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 1 cut(s) 601
AluBI AGCT 5 cut(s) 67, 121, 397, 587, 871
AluI AGCT 5 cut(s) 67, 121, 397, 587, 871
Alw26I GTCTC 2 cut(s) 20, 319
AlwNI CAGNNNCTG 3 cut(s) 203, 239, 805
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 4 cut(s) 394, 584, 805, 898
ApoI RAATTY 1 cut(s) 39
AseI ATTAAT 1 cut(s) 465
Asp700I GAANNNNTTC 1 cut(s) 610
Asp718I GGTACC 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 622, 772
AsuHPI GGTGA 1 cut(s) 491
AvaII GGWCC 2 cut(s) 622, 772
BaeGI GKGCMC 1 cut(s) 954
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 353
BbsI GAAGAC 2 cut(s) 254, 972
BbvI GCAGC 4 cut(s) 406, 596, 792, 910
BccI CCATC 3 cut(s) 139, 181, 750
BciT130I CCWGG 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 20, 319
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 3 cut(s) 294, 306, 630
BfmI CTRYAG 3 cut(s) 168, 195, 690
BglII AGATCT 1 cut(s) 262
BisI GCNGC 4 cut(s) 395, 585, 806, 899
BlsI GCNGC 4 cut(s) 396, 586, 807, 900
Bme1390I CCNGG 1 cut(s) 603
Bme18I GGWCC 2 cut(s) 622, 772
BmgT120I GGNCC 2 cut(s) 622, 772
BmiI GGNNCC 4 cut(s) 112, 319, 624, 664
BmrFI CCNGG 1 cut(s) 603
BmrI ACTGGG 2 cut(s) 366, 736
BmsI GCATC 2 cut(s) 418, 792
BmuI ACTGGG 2 cut(s) 366, 736
BpiI GAAGAC 2 cut(s) 254, 972
BplI GAGNNNNNCTC 2 cut(s) 340, 372
BpmI CTGGAG 1 cut(s) 899
Bpu10I CCTNAGC 1 cut(s) 122
BsaI GGTCTC 2 cut(s) 20, 319
BsaJI CCNNGG 2 cut(s) 279, 819
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 3 cut(s) 535, 659, 976
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 6 cut(s) 233, 372, 578, 649, 731, 882
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 2 cut(s) 279, 819
BseGI GGATG 2 cut(s) 192, 673
BseLI CCNNNNNNNGG 3 cut(s) 535, 659, 976
BseMII CTCAG 5 cut(s) 195, 327, 462, 821, 974
BseNI ACTGG 6 cut(s) 233, 372, 578, 649, 731, 882
BseRI GAGGAG 2 cut(s) 345, 808
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 4 cut(s) 406, 596, 792, 910
BsgI GTGCAG 1 cut(s) 707
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 2 cut(s) 812, 974
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 317
BsiEI CGRYCG 2 cut(s) 812, 974
BsiSI CCGG 2 cut(s) 560, 620
BslFI GGGAC 1 cut(s) 608
BslI CCNNNNNNNGG 3 cut(s) 535, 659, 976
BsmAI GTCTC 2 cut(s) 20, 319
BsmFI GGGAC 1 cut(s) 608
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 2 cut(s) 20, 319
Bsp1286I GDGCHC 2 cut(s) 353, 954
Bsp143I GATC 3 cut(s) 262, 860, 971
BspACI CCGC 4 cut(s) 108, 599, 626, 808
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 5 cut(s) 196, 328, 463, 822, 973
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 4 cut(s) 112, 319, 624, 664
BspQI GCTCTTC 1 cut(s) 726
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 2 cut(s) 20, 319
BsrFI RCCGGY 1 cut(s) 559
BsrI ACTGG 6 cut(s) 233, 372, 578, 649, 731, 882
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 2 cut(s) 279, 819
BssMI GATC 3 cut(s) 262, 860, 971
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 2 cut(s) 253, 694
Bst6I CTCTTC 1 cut(s) 726
BstC8I GCNNGC 3 cut(s) 460, 511, 561
BstDEI CTNAG 7 cut(s) 122, 204, 336, 471, 494, 830, 960
BstF5I GGATG 2 cut(s) 192, 673
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 3 cut(s) 265, 863, 974
BstMAI GTCTC 2 cut(s) 20, 319
BstMBI GATC 3 cut(s) 262, 860, 971
BstMCI CGRYCG 2 cut(s) 812, 974
BstMWI GCNNNNNNNGC 2 cut(s) 811, 907
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 601
BstSFI CTRYAG 3 cut(s) 168, 195, 690
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 4 cut(s) 406, 596, 792, 910
BstV2I GAAGAC 2 cut(s) 254, 972
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BsuRI GGCC 1 cut(s) 343
BtsCI GGATG 2 cut(s) 192, 673
BtsIMutI CAGTG 6 cut(s) 181, 258, 585, 699, 724, 875
Cac8I GCNNGC 3 cut(s) 460, 511, 561
CaiI CAGNNNCTG 3 cut(s) 203, 239, 805
CciI TCATGA 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 2 cut(s) 622, 772
Csp6I GTAC 3 cut(s) 318, 444, 642
CviAII CATG 4 cut(s) 218, 447, 503, 785
CviQI GTAC 3 cut(s) 318, 444, 642
DdeI CTNAG 7 cut(s) 122, 204, 336, 471, 494, 830, 960
DpnI GATC 3 cut(s) 264, 862, 973
DpnII GATC 3 cut(s) 262, 860, 971
DraI TTTAAA 1 cut(s) 946
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
EciI GGCGGA 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 353
Eco31I GGTCTC 2 cut(s) 20, 319
Eco47I GGWCC 2 cut(s) 622, 772
Eco57I CTGAAG 3 cut(s) 125, 441, 597
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 4 cut(s) 221, 450, 506, 788
FaqI GGGAC 1 cut(s) 608
FatI CATG 4 cut(s) 217, 446, 502, 784
FauI CCCGC 1 cut(s) 633
FauNDI CATATG 1 cut(s) 97
Fnu4HI GCNGC 4 cut(s) 395, 585, 806, 899
FokI GGATG 2 cut(s) 199, 660
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 4 cut(s) 395, 585, 806, 899
FspBI CTAG 3 cut(s) 294, 306, 630
GluI GCNGC 4 cut(s) 395, 585, 806, 899
GsuI CTGGAG 1 cut(s) 899
HaeIII GGCC 1 cut(s) 343
HapII CCGG 2 cut(s) 560, 620
Hin1II CATG 4 cut(s) 221, 450, 506, 788
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 4 cut(s) 164, 200, 206, 738
HpaII CCGG 2 cut(s) 560, 620
HphI GGTGA 1 cut(s) 491
Hpy188I TCNGA 7 cut(s) 144, 205, 421, 437, 480, 781, 976
Hpy188III TCNNGA 4 cut(s) 56, 218, 799, 962
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 3 cut(s) 95, 102, 621
HpyCH4III ACNGT 2 cut(s) 253, 694
HpyCH4IV ACGT 1 cut(s) 903
HpyF10VI GCNNNNNNNGC 2 cut(s) 811, 907
HpyF3I CTNAG 7 cut(s) 122, 204, 336, 471, 494, 830, 960
HpySE526I ACGT 1 cut(s) 903
Hsp92II CATG 4 cut(s) 221, 450, 506, 788
KpnI GGTACC 1 cut(s) 321
KroI GCCGGC 1 cut(s) 559
KroNI GCCGGC 1 cut(s) 561
Kzo9I GATC 3 cut(s) 262, 860, 971
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 4 cut(s) 406, 596, 792, 910
LweI GCATC 2 cut(s) 418, 792
MaeI CTAG 3 cut(s) 294, 306, 630
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 1 cut(s) 497
MalI GATC 3 cut(s) 264, 862, 973
MboI GATC 3 cut(s) 262, 860, 971
MboII GAAGA 5 cut(s) 80, 259, 678, 743, 977
MfeI CAATTG 1 cut(s) 746
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 353, 954
MluCI AATT 4 cut(s) 39, 134, 746, 864
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 831
MroNI GCCGGC 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 3 cut(s) 257, 465, 945
MspI CCGG 2 cut(s) 560, 620
MspR9I CCNGG 1 cut(s) 603
MunI CAATTG 1 cut(s) 746
MvaI CCWGG 1 cut(s) 603
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 811, 907
NaeI GCCGGC 1 cut(s) 561
NdeI CATATG 1 cut(s) 97
NdeII GATC 3 cut(s) 262, 860, 971
NgoMIV GCCGGC 1 cut(s) 559
NlaIII CATG 4 cut(s) 221, 450, 506, 788
NlaIV GGNNCC 4 cut(s) 112, 319, 624, 664
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 1 cut(s) 450
PagI TCATGA 1 cut(s) 217
PciI ACATGT 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 726
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PdiI GCCGGC 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 3 cut(s) 164, 200, 738
PkrI GCNGC 4 cut(s) 396, 586, 807, 900
Ple19I CGATCG 1 cut(s) 974
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PscI ACATGT 1 cut(s) 446
PshBI ATTAAT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 4 cut(s) 112, 319, 624, 664
PspPI GGNCC 2 cut(s) 622, 772
PstNI CAGNNNCTG 3 cut(s) 203, 239, 805
PsuI RGATCY 1 cut(s) 262
PvuI CGATCG 1 cut(s) 974
RsaI GTAC 3 cut(s) 319, 445, 643
RsaNI GTAC 3 cut(s) 318, 444, 642
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 3 cut(s) 257, 465, 945
SatI GCNGC 4 cut(s) 395, 585, 806, 899
Sau3AI GATC 3 cut(s) 262, 860, 971
Sau96I GGNCC 2 cut(s) 622, 772
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 2 cut(s) 353, 954
SfaNI GCATC 2 cut(s) 418, 792
SfcI CTRYAG 3 cut(s) 168, 195, 690
SinI GGWCC 2 cut(s) 622, 772
SpeI ACTAGT 1 cut(s) 293
Sse9I AATT 4 cut(s) 39, 134, 746, 864
SsiI CCGC 4 cut(s) 108, 599, 626, 808
SspMI CTAG 3 cut(s) 294, 306, 630
StyD4I CCNGG 1 cut(s) 601
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 253, 694
TaiI ACGT 1 cut(s) 906
TaqI TCGA 1 cut(s) 55
TaqII GACCGA 1 cut(s) 984
TasI AATT 4 cut(s) 39, 134, 746, 864
TatI WGTACW 1 cut(s) 443
TfiI GAWTC 3 cut(s) 164, 200, 738
Tru1I TTAA 3 cut(s) 257, 465, 945
Tru9I TTAA 3 cut(s) 257, 465, 945
TscAI CASTG 6 cut(s) 181, 258, 585, 699, 731, 882
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 4 cut(s) 394, 584, 805, 898
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 2 cut(s) 84, 206
TspRI CASTG 6 cut(s) 181, 258, 585, 699, 731, 882
VpaK11BI GGWCC 2 cut(s) 622, 772
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 1 cut(s) 39
XceI RCATGY 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 3 cut(s) 294, 306, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.