Rroxscaffold_7G00187760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000902
Physical Location & Seq
Reverse (-)
27211038 .. 27215529
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187760.1

Sequence Viewer

Length: 1779 bp
ATGGTTCAGCATACCATAGATTCTAAAACCAATGAATATGGGCTTGGAACTGCTGAGGCCGAGTTGTCTATTCATGATAAGCAGTTATCAGTTCCTGTAAAGAAGACAGTTTTAAGAGATGTGCAAAATGGCAATAGAATTCCAGCGCCCGGCTCTGCTGGGAGCTCTTCTCCATTGAAGGATAAGATACCTAATAGTAATTCCCCTAGAGTTTCTGGCACCAAAAGACCTTTATCTGAGTGCCAGATGATCCCTCCTGAACAGCAACCCCCCAGTAGTACTTTTGCAAATGGGCATCTTGTCTATGTTCGCAGGAAATCAGAAGCTGACGCAGTCAGAAGTAGTGCTAATGAGAATGCAAGCATTAATGCTGATGGTCAGGCATCGAGGAAACTTAGCAACCAAGAGGCAAGCACCGGACCGATATCCCAGACAAAGGAGCTAACAGTTCATAGTGCTAATGATAATGCAAATGTTAATGCTGGTGGTGAGGTGTTGAAGATAATTAGCCACCAAGAGGCAGGCAAAGGCCAGATATCCCAAACAAAGGAGCCAACAGTTCACAGTGCTAATGATAATGCAAACGTTAATGCTGATGGTGAGGTGTTGAAGATAATTAGCCACCAAGAGGCAGGCAAAGGACAGATATCCCAAACAAAGGAGCCAACAGTTCATAGTGCTAATGATAATGCAAGCATTAATGCTGATGGTCAGGCATCGAGGAAACTTAGCAACCAAGAGGCAAGCACCCGACCGATATCCCAAACAAAGGAGCCAACAGTTCACAGTGCTAATGATAATGCAAACATTAATGCTGATTGTGAGGTGTTGAGGATAGTTAGCCACCAAGAGGCAGGCACCGGACCCATATCCCAAACAAAGGAGCCAGAAGTTCATTGTGCTGATGATAATGCAAGCATTAATGCCGATTGTCAGGTATTGAGGAAACTTAGCAATGAAGAGGCAAGCAGCGTACCGATATCCCAAAGAAAGGAGCCACAAGTTCAGTTTACTAATGATAATGCAAGCATTAATGCTGATTGTGAGGTGTCGAGGATAGTTAGCCACCAAGAGGCAGGCACGGGACCCATATCCAAAATAAAGGAGCCAAAAGTTCATTGTGCTAATGATAATGCAAGAATTAATGCTGATTGTGAGATATTGAGGATGGTTAACCACCAAGAGGCAGGCTCCGGACCTGTATCCAAAACAAAGGAGCCAAAAGTTCATTTTGCTAATGATAATGCGAGCATGAAGGCTGATTGTCAGGTATCGAGGATAAATAGCCACCAAGAGGCAAGCAGCATACCCATATCCCAAAGGAAGGAGCCAACAGTTCATAACATTCCGGCACTTGCACCTTTTCCAAGGGAAGCTGCAGCCATCCCTCCTGGAAAGCCATCAGTTTCTCTGCCACACAGTAAATCTGATCTAAGAGGAGCACCGGCAGGATCCACTTGCCAACCTCTTGCTTCTGCTACCCCTTCATTGGGTAGTTCAAAGGGACTTAAAAATCTGCATTGGGAAGAGAGGTTTCATCAACTGCAGTTGCTATTGAGGAAATTGGACCAATCAGACCATGAGGATTATCTCAAGATGCTGCGGTCACTCTCCTCGGTTGAACTAAGCAGACATGCGGTTGAGTTGGAAAAGAGATCAATTCAGCTTTCACTGGAGGAAGCAAAAGAGTTGAAGCGAGTTGGGGTGTTGAATGTTCTGGGAAAACCTATGAAGCCTTTTAAAGTGCCCTCAACACTTCAAGACAGATCTGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN52

Protein Analysis

592

Amino Acids

63.77

Weight (kDa)

7.09

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 218, 857
AccIII TCCGGA 1 cut(s) 1193
AciI CCGC 2 cut(s) 1603, 1637
AclI AACGTT 1 cut(s) 585
AclWI GGATC 3 cut(s) 244, 1446, 1459
AcsI RAATTY 1 cut(s) 138
AfaI GTAC 2 cut(s) 280, 975
AgsI TTSAA 8 cut(s) 178, 499, 610, 1500, 1622, 1693, 1711, 1760
AjnI CCWGG 1 cut(s) 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 5 cut(s) 165, 326, 442, 1376, 1666
AluI AGCT 5 cut(s) 165, 326, 442, 1376, 1666
Alw21I GWGCWC 2 cut(s) 167, 1444
AlwI GGATC 3 cut(s) 244, 1446, 1459
AlwNI CAGNNNCTG 2 cut(s) 95, 326
Aor13HI TCCGGA 1 cut(s) 1193
AoxI GGCC 2 cut(s) 57, 529
ApeKI GCWGC 5 cut(s) 969, 1302, 1376, 1379, 1600
ApoI RAATTY 1 cut(s) 138
AseI ATTAAT 6 cut(s) 366, 699, 810, 921, 1032, 1143
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 5 cut(s) 419, 863, 1085, 1196, 1567
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 500, 611
AvaII GGWCC 5 cut(s) 419, 863, 1085, 1196, 1567
BaeGI GKGCMC 1 cut(s) 1749
BamHI GGATCC 1 cut(s) 1451
BanI GGYRCC 2 cut(s) 218, 857
BanII GRGCYC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 2 cut(s) 167, 1444
BbvCI CCTCAGC 1 cut(s) 54
BbvI GCAGC 5 cut(s) 981, 1314, 1363, 1391, 1587
BccI CCATC 6 cut(s) 368, 590, 701, 1162, 1391, 1408
BciT130I CCWGG 1 cut(s) 1392
BciVI GTATCC 1 cut(s) 1213
BcnI CCSGG 1 cut(s) 150
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 2 cut(s) 1377, 1544
BfoI RGCGCY 1 cut(s) 149
BfuI GTATCC 1 cut(s) 1213
BglII AGATCT 1 cut(s) 1766
BisI GCNGC 5 cut(s) 970, 1303, 1377, 1380, 1601
BlsI GCNGC 5 cut(s) 971, 1304, 1378, 1381, 1602
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 150, 1392
Bme18I GGWCC 5 cut(s) 419, 863, 1085, 1196, 1567
BmgT120I GGNCC 5 cut(s) 419, 863, 1085, 1196, 1567
BmrFI CCNGG 2 cut(s) 150, 1392
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 4 cut(s) 304, 392, 725, 1587
BmuI ACTGGG 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 110
BplI GAGNNNNNCTC 4 cut(s) 154, 186, 1175, 1207
BpmI CTGGAG 1 cut(s) 1694
Bpu10I CCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 1577
BpuMI CCSGG 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 1367, 1614
BsaWI WCCGGW 3 cut(s) 416, 860, 1193
Bse118I RCCGGY 1 cut(s) 1444
Bse1I ACTGG 2 cut(s) 273, 1677
Bse3DI GCAATG 1 cut(s) 961
BseAI TCCGGA 1 cut(s) 1193
BseBI CCWGG 1 cut(s) 1392
BseDI CCNNGG 2 cut(s) 1367, 1614
BseGI GGATG 2 cut(s) 1173, 1383
BseMI GCAATG 1 cut(s) 961
BseMII CTCAG 3 cut(s) 45, 228, 1761
BseNI ACTGG 2 cut(s) 273, 1677
BseRI GAGGAG 2 cut(s) 1452, 1603
BseSI GKGCMC 1 cut(s) 1749
BseXI GCAGC 5 cut(s) 981, 1314, 1363, 1391, 1587
BseYI CCCAGC 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 755
BshFI GGCC 2 cut(s) 59, 531
BshNI GGYRCC 2 cut(s) 218, 857
BsiEI CGRYCG 1 cut(s) 755
BsiHKAI GWGCWC 2 cut(s) 167, 1444
BsiSI CCGG 6 cut(s) 150, 417, 861, 1194, 1349, 1445
BslFI GGGAC 2 cut(s) 1098, 1518
BsmFI GGGAC 2 cut(s) 1098, 1518
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 2 cut(s) 59, 531
Bsp1286I GDGCHC 3 cut(s) 167, 1444, 1749
Bsp13I TCCGGA 1 cut(s) 1193
Bsp143I GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
BspACI CCGC 2 cut(s) 1603, 1637
BspANI GGCC 2 cut(s) 59, 531
BspCNI CTCAG 3 cut(s) 46, 229, 1762
BspEI TCCGGA 1 cut(s) 1193
BspHI TCATGA 1 cut(s) 73
BspMAI CTGCAG 2 cut(s) 1381, 1548
BspPI GGATC 3 cut(s) 244, 1446, 1459
BspQI GCTCTTC 1 cut(s) 172
BspT107I GGYRCC 2 cut(s) 218, 857
BsrDI GCAATG 1 cut(s) 961
BsrFI RCCGGY 1 cut(s) 1444
BsrI ACTGG 2 cut(s) 273, 1677
BssAI RCCGGY 1 cut(s) 1444
BssECI CCNNGG 2 cut(s) 1367, 1614
BssMI GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
BssT1I CCWWGG 1 cut(s) 1367
Bst2UI CCWGG 1 cut(s) 1392
Bst4CI ACNGT 9 cut(s) 109, 448, 559, 566, 670, 781, 788, 1336, 1421
Bst6I CTCTTC 3 cut(s) 172, 954, 1521
BstDEI CTNAG 8 cut(s) 54, 237, 395, 728, 950, 1433, 1625, 1770
BstF5I GGATG 2 cut(s) 1173, 1383
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 5 cut(s) 252, 1432, 1454, 1658, 1769
BstMBI GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
BstMCI CGRYCG 1 cut(s) 755
BstNI CCWGG 1 cut(s) 1392
BstNSI RCATGY 1 cut(s) 1637
BstSCI CCNGG 2 cut(s) 148, 1390
BstSFI CTRYAG 2 cut(s) 1377, 1544
BstSLI GKGCMC 1 cut(s) 1749
BstV1I GCAGC 5 cut(s) 981, 1314, 1363, 1391, 1587
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 2 cut(s) 1451, 1766
BstYI RGATCY 2 cut(s) 1451, 1766
BsuI GTATCC 1 cut(s) 1213
BsuRI GGCC 2 cut(s) 59, 531
BtsCI GGATG 2 cut(s) 1173, 1383
BtsIMutI CAGTG 3 cut(s) 571, 793, 1670
CaiI CAGNNNCTG 2 cut(s) 95, 326
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 148
Cfr10I RCCGGY 1 cut(s) 1444
Cfr13I GGNCC 5 cut(s) 419, 863, 1085, 1196, 1567
CpoI CGGWCCG 1 cut(s) 419
CseI GACGC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 279, 974
CspI CGGWCCG 1 cut(s) 419
CviAII CATG 4 cut(s) 74, 1252, 1580, 1634
CviQI GTAC 2 cut(s) 279, 974
DdeI CTNAG 8 cut(s) 54, 237, 395, 728, 950, 1433, 1625, 1770
DpnI GATC 5 cut(s) 251, 1431, 1453, 1657, 1768
DpnII GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
DraI TTTAAA 1 cut(s) 1741
Eam1104I CTCTTC 3 cut(s) 172, 954, 1521
EarI CTCTTC 3 cut(s) 172, 954, 1521
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 1367
Eco24I GRGCYC 1 cut(s) 167
Eco32I GATATC 5 cut(s) 426, 537, 648, 759, 981
Eco47I GGWCC 5 cut(s) 419, 863, 1085, 1196, 1567
Eco53kI GAGCTC 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 1085
EcoRI GAATTC 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 1390
EcoRV GATATC 5 cut(s) 426, 537, 648, 759, 981
EcoT14I CCWWGG 1 cut(s) 1367
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 1367
FaeI CATG 4 cut(s) 77, 1255, 1583, 1637
FaqI GGGAC 2 cut(s) 1098, 1518
FatI CATG 4 cut(s) 73, 1251, 1579, 1633
Fnu4HI GCNGC 5 cut(s) 970, 1303, 1377, 1380, 1601
FokI GGATG 2 cut(s) 1180, 1370
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 5 cut(s) 970, 1303, 1377, 1380, 1601
FspBI CTAG 1 cut(s) 207
GlaI GCGC 1 cut(s) 147
GluI GCNGC 5 cut(s) 970, 1303, 1377, 1380, 1601
GsaI CCCAGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 1694
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 59, 531
HapII CCGG 6 cut(s) 150, 417, 861, 1194, 1349, 1445
HgaI GACGC 1 cut(s) 338
HhaI GCGC 1 cut(s) 148
Hin1II CATG 4 cut(s) 77, 1255, 1583, 1637
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 1174
HindII GTYRAC 1 cut(s) 1174
HinfI GANTC 1 cut(s) 20
HpaI GTTAAC 1 cut(s) 1174
HpaII CCGG 6 cut(s) 150, 417, 861, 1194, 1349, 1445
HphI GGTGA 2 cut(s) 500, 611
Hpy166II GTNNAC 4 cut(s) 562, 784, 1011, 1174
Hpy188I TCNGA 6 cut(s) 238, 322, 338, 1429, 1576, 1771
Hpy188III TCNNGA 5 cut(s) 74, 257, 1194, 1594, 1760
Hpy8I GTNNAC 4 cut(s) 562, 784, 1011, 1174
HpyAV CCTTC 4 cut(s) 172, 1249, 1318, 1494
HpyCH4III ACNGT 9 cut(s) 109, 448, 559, 566, 670, 781, 788, 1336, 1421
HpyCH4IV ACGT 1 cut(s) 585
HpyF3I CTNAG 8 cut(s) 54, 237, 395, 728, 950, 1433, 1625, 1770
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 4 cut(s) 77, 1255, 1583, 1637
HspAI GCGC 1 cut(s) 146
KflI GGGWCCC 1 cut(s) 1085
Kpn2I TCCGGA 1 cut(s) 1193
KspAI GTTAAC 1 cut(s) 1174
Kzo9I GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
LguI GCTCTTC 1 cut(s) 172
Lsp1109I GCAGC 5 cut(s) 981, 1314, 1363, 1391, 1587
LweI GCATC 4 cut(s) 304, 392, 725, 1587
MaeI CTAG 1 cut(s) 207
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 1 cut(s) 1605
MalI GATC 5 cut(s) 251, 1431, 1453, 1657, 1768
MboI GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
MboII GAAGA 6 cut(s) 115, 159, 511, 622, 971, 1538
MflI RGATCY 2 cut(s) 1451, 1766
MhlI GDGCHC 3 cut(s) 167, 1444, 1749
MluCI AATT 7 cut(s) 138, 199, 504, 615, 1140, 1562, 1659
MmeI TCCRAC 1 cut(s) 1626
MroI TCCGGA 1 cut(s) 1193
MspI CCGG 6 cut(s) 150, 417, 861, 1194, 1349, 1445
MspR9I CCNGG 2 cut(s) 150, 1392
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 1392
NciI CCSGG 1 cut(s) 150
NdeII GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
NlaIII CATG 4 cut(s) 77, 1255, 1583, 1637
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 1605
NspI RCATGY 1 cut(s) 1637
PagI TCATGA 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 172
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 1390
PkrI GCNGC 5 cut(s) 971, 1304, 1378, 1381, 1602
PpuMI RGGWCCY 1 cut(s) 1085
PshBI ATTAAT 6 cut(s) 366, 699, 810, 921, 1032, 1143
Psp124BI GAGCTC 1 cut(s) 167
Psp1406I AACGTT 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 1085
Psp6I CCWGG 1 cut(s) 1390
PspFI CCCAGC 1 cut(s) 158
PspGI CCWGG 1 cut(s) 1390
PspPI GGNCC 5 cut(s) 419, 863, 1085, 1196, 1567
PspPPI RGGWCCY 1 cut(s) 1085
PstI CTGCAG 2 cut(s) 1381, 1548
PstNI CAGNNNCTG 2 cut(s) 95, 326
PsuI RGATCY 2 cut(s) 1451, 1766
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 2 cut(s) 280, 975
RsaNI GTAC 2 cut(s) 279, 974
Rsr2I CGGWCCG 1 cut(s) 419
RsrII CGGWCCG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 172
SatI GCNGC 5 cut(s) 970, 1303, 1377, 1380, 1601
Sau3AI GATC 5 cut(s) 249, 1429, 1451, 1655, 1766
Sau96I GGNCC 5 cut(s) 419, 863, 1085, 1196, 1567
ScaI AGTACT 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 150, 1392
SduI GDGCHC 3 cut(s) 167, 1444, 1749
SfaNI GCATC 4 cut(s) 304, 392, 725, 1587
SfcI CTRYAG 2 cut(s) 1377, 1544
SinI GGWCC 5 cut(s) 419, 863, 1085, 1196, 1567
SmlI CTYRAG 1 cut(s) 1592
SmoI CTYRAG 1 cut(s) 1592
Sse9I AATT 7 cut(s) 138, 199, 504, 615, 1140, 1562, 1659
SsiI CCGC 2 cut(s) 1603, 1637
SspMI CTAG 1 cut(s) 207
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 2 cut(s) 148, 1390
StyI CCWWGG 1 cut(s) 1367
TaaI ACNGT 9 cut(s) 109, 448, 559, 566, 670, 781, 788, 1336, 1421
TaiI ACGT 1 cut(s) 588
TaqI TCGA 4 cut(s) 386, 719, 1052, 1274
TaqII GACCGA 2 cut(s) 436, 769
TasI AATT 7 cut(s) 138, 199, 504, 615, 1140, 1562, 1659
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 1 cut(s) 20
TscAI CASTG 3 cut(s) 571, 793, 1677
TseFI GTSAC 1 cut(s) 1605
TseI GCWGC 5 cut(s) 969, 1302, 1376, 1379, 1600
Tsp45I GTSAC 1 cut(s) 1605
TspRI CASTG 3 cut(s) 571, 793, 1677
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 5 cut(s) 419, 863, 1085, 1196, 1567
VspI ATTAAT 6 cut(s) 366, 699, 810, 921, 1032, 1143
XapI RAATTY 1 cut(s) 138
XceI RCATGY 1 cut(s) 1637
XspI CTAG 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.