Rorug07G0153100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000902
Physical Location & Seq
Reverse (-)
12155700 .. 12157487
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0153100.1

Sequence Viewer

Length: 603 bp
ATGTCTGAGAAAGCAGGGCACGATGCAGAAACACACCCTTTCCACGCCGATGAAGAGCGCAAGCGCCAGAAGAGGATCAAGATGTACAAATACATCGGTATTTTCATAGTGTTTCAAATCGTAGTGGTGACCGTTTTCGCACTCACGGTCATGAAAGTCAAGACCCCGAAAATCCGACTAGGCAACATCTACGTCCAGTCACTCACAGCCAACGCCGCAACCCCCTCATTCGACATGAACTTCGCAACCCAAATCCGGGTCAGGAACCCCAACTGGGGACCTTACAAGTTCGAGGCCGGCGTAGTCACGTTCATGTACCAAGGTGTCCCCGTTGGACAAGTTTACATCCCCGAGGGCAAAGCTGGGATGAGGTCGACCAAGAAAATTGATGTGATGGTGAGTGTGAGCTCGGCCTCGTTGCCCGGGAACACAATGCTCGGGACTGAGCTGAACAATGGGCTATTGATGCTGACCAGCACGGCCACGGTGACCGGGAAAGTAGAGTTGATGTTGATCATGAAGAAGAGGAAGTCTGCGGACATGTTGTGCTCTATGGTGTTTAGTGTGGCCACAAACACTGTCCAGAATTTGGAGTGCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN52

Protein Analysis

200

Amino Acids

22.21

Weight (kDa)

9.61

Isoelectric Point (pI)

28.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 85 - 176 8.6e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 589
AccI GTMKAC 1 cut(s) 374
AciI CCGC 2 cut(s) 216, 536
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 2 cut(s) 480, 567
AcsI RAATTY 1 cut(s) 586
AfaI GTAC 2 cut(s) 86, 317
AfiI CCNNNNNNNGG 5 cut(s) 255, 256, 274, 275, 589
AflIII ACRYGT 1 cut(s) 540
AgsI TTSAA 1 cut(s) 116
AluBI AGCT 3 cut(s) 362, 408, 448
AluI AGCT 3 cut(s) 362, 408, 448
Alw21I GWGCWC 2 cut(s) 410, 551
AlwI GGATC 1 cut(s) 83
Ama87I CYCGRG 3 cut(s) 350, 422, 437
AoxI GGCC 4 cut(s) 294, 411, 480, 567
ApoI RAATTY 1 cut(s) 586
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AspLEI GCGC 2 cut(s) 60, 66
AspS9I GGNCC 1 cut(s) 278
AsuC2I CCSGG 4 cut(s) 257, 423, 424, 493
AsuHPI GGTGA 3 cut(s) 139, 409, 499
AvaI CYCGRG 3 cut(s) 350, 422, 437
AvaII GGWCC 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 21
BalI TGGCCA 1 cut(s) 569
BanII GRGCYC 1 cut(s) 410
Bbv12I GWGCWC 2 cut(s) 410, 551
BccI CCATC 1 cut(s) 388
BceAI ACGGC 1 cut(s) 495
BclI TGATCA 1 cut(s) 513
BcnI CCSGG 4 cut(s) 257, 423, 424, 493
BfaI CTAG 1 cut(s) 179
BfoI RGCGCY 1 cut(s) 67
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 4 cut(s) 257, 423, 424, 493
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 3 cut(s) 350, 422, 437
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 266, 279
BmrFI CCNGG 4 cut(s) 257, 423, 424, 493
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 2 cut(s) 13, 456
BmuI ACTGGG 1 cut(s) 283
BpuMI CCSGG 4 cut(s) 257, 423, 424, 493
BsaBI GATNNNNATC 1 cut(s) 512
BsaJI CCNNGG 4 cut(s) 319, 351, 422, 483
BsaXI ACNNNNNCTCC 1 cut(s) 584
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 256, 274, 275, 589
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 2 cut(s) 196, 278
Bse8I GATNNNNATC 1 cut(s) 512
BseDI CCNNGG 4 cut(s) 319, 351, 422, 483
BseGI GGATG 2 cut(s) 345, 372
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 5 cut(s) 255, 256, 274, 275, 589
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 2 cut(s) 196, 278
BseSI GKGCMC 1 cut(s) 21
BseYI CCCAGC 1 cut(s) 362
BshFI GGCC 4 cut(s) 296, 413, 482, 569
BsiHKAI GWGCWC 2 cut(s) 410, 551
BsiHKCI CYCGRG 3 cut(s) 350, 422, 437
BsiSI CCGG 4 cut(s) 256, 297, 423, 492
BslFI GGGAC 3 cut(s) 291, 311, 454
BslI CCNNNNNNNGG 5 cut(s) 255, 256, 274, 275, 589
BsmFI GGGAC 3 cut(s) 291, 311, 454
BsnI GGCC 4 cut(s) 296, 413, 482, 569
BsoBI CYCGRG 3 cut(s) 350, 422, 437
Bsp1286I GDGCHC 3 cut(s) 21, 410, 551
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 2 cut(s) 75, 513
BspACI CCGC 2 cut(s) 216, 536
BspANI GGCC 4 cut(s) 296, 413, 482, 569
BspCNI CTCAG 1 cut(s) 436
BspHI TCATGA 2 cut(s) 150, 516
BspLI GGNNCC 2 cut(s) 266, 279
BspPI GGATC 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 296
BsrGI TGTACA 1 cut(s) 84
BsrI ACTGG 2 cut(s) 196, 278
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 4 cut(s) 319, 351, 422, 483
BssMI GATC 2 cut(s) 75, 513
BssT1I CCWWGG 1 cut(s) 319
Bst4CI ACNGT 4 cut(s) 133, 148, 487, 580
Bst6I CTCTTC 3 cut(s) 48, 65, 518
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 62, 298
BstDEI CTNAG 2 cut(s) 6, 444
BstDSI CCRYGG 1 cut(s) 483
BstEII GGTNACC 2 cut(s) 127, 487
BstF5I GGATG 2 cut(s) 345, 372
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 2 cut(s) 60, 66
BstKTI GATC 2 cut(s) 78, 516
BstMBI GATC 2 cut(s) 75, 513
BstMWI GCNNNNNNNGC 2 cut(s) 215, 466
BstNSI RCATGY 1 cut(s) 544
BstPI GGTNACC 2 cut(s) 127, 487
BstSCI CCNGG 4 cut(s) 255, 421, 422, 491
BstSLI GKGCMC 1 cut(s) 21
BsuRI GGCC 4 cut(s) 296, 413, 482, 569
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 2 cut(s) 345, 372
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 2 cut(s) 62, 298
CciI TCATGA 2 cut(s) 150, 516
CfoI GCGC 2 cut(s) 60, 66
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 278
Cfr9I CCCGGG 1 cut(s) 422
Csp6I GTAC 2 cut(s) 85, 316
CviAII CATG 5 cut(s) 151, 235, 313, 517, 541
CviJI RGCY 9 cut(s) 209, 296, 362, 408, 413, 448, 460, 482, 569
CviKI_1 RGCY 9 cut(s) 209, 296, 362, 408, 413, 448, 460, 482, 569
CviQI GTAC 2 cut(s) 85, 316
DdeI CTNAG 2 cut(s) 6, 444
DpnI GATC 2 cut(s) 77, 515
DpnII GATC 2 cut(s) 75, 513
EaeI YGGCCR 2 cut(s) 480, 567
Eam1104I CTCTTC 3 cut(s) 48, 65, 518
EarI CTCTTC 3 cut(s) 48, 65, 518
Ecl136II GAGCTC 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 410
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 408
Eco88I CYCGRG 3 cut(s) 350, 422, 437
Eco91I GGTNACC 2 cut(s) 127, 487
EcoICRI GAGCTC 1 cut(s) 408
EcoO109I RGGNCCY 1 cut(s) 278
EcoO65I GGTNACC 2 cut(s) 127, 487
EcoT14I CCWWGG 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 5 cut(s) 154, 238, 316, 520, 544
FaiI YATR 7 cut(s) 107, 152, 236, 314, 518, 542, 554
FaqI GGGAC 3 cut(s) 291, 311, 454
FatI CATG 5 cut(s) 150, 234, 312, 516, 540
FbaI TGATCA 1 cut(s) 513
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 332, 379
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 1 cut(s) 179
GlaI GCGC 2 cut(s) 59, 65
GluI GCNGC 1 cut(s) 216
GsaI CCCAGC 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 4 cut(s) 296, 413, 482, 569
HapII CCGG 4 cut(s) 256, 297, 423, 492
HhaI GCGC 2 cut(s) 60, 66
Hin1II CATG 5 cut(s) 154, 238, 316, 520, 544
Hin6I GCGC 2 cut(s) 58, 64
HinP1I GCGC 2 cut(s) 58, 64
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HpaII CCGG 4 cut(s) 256, 297, 423, 492
HphI GGTGA 3 cut(s) 139, 409, 499
Hpy166II GTNNAC 2 cut(s) 343, 375
Hpy188I TCNGA 2 cut(s) 7, 176
Hpy188III TCNNGA 7 cut(s) 79, 151, 160, 262, 439, 517, 583
Hpy8I GTNNAC 2 cut(s) 343, 375
HpyCH4III ACNGT 4 cut(s) 133, 148, 487, 580
HpyCH4IV ACGT 2 cut(s) 192, 308
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 466
HpyF3I CTNAG 2 cut(s) 6, 444
HpySE526I ACGT 2 cut(s) 192, 308
Hsp92II CATG 5 cut(s) 154, 238, 316, 520, 544
HspAI GCGC 2 cut(s) 58, 64
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 513
Kzo9I GATC 2 cut(s) 75, 513
LguI GCTCTTC 1 cut(s) 48
LweI GCATC 2 cut(s) 13, 456
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 192, 308
MaeIII GTNAC 4 cut(s) 127, 198, 304, 487
MalI GATC 2 cut(s) 77, 515
MboI GATC 2 cut(s) 75, 513
MboII GAAGA 4 cut(s) 65, 82, 532, 535
MhlI GDGCHC 3 cut(s) 21, 410, 551
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 2 cut(s) 384, 586
MluNI TGGCCA 1 cut(s) 569
MmeI TCCRAC 2 cut(s) 199, 313
MnlI CCTC 7 cut(s) 66, 235, 286, 346, 363, 424, 519
Mox20I TGGCCA 1 cut(s) 569
MroNI GCCGGC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 569
MslI CAYNNNNRTG 3 cut(s) 48, 149, 311
Msp20I TGGCCA 1 cut(s) 569
MspI CCGG 4 cut(s) 256, 297, 423, 492
MspR9I CCNGG 4 cut(s) 257, 423, 424, 493
MwoI GCNNNNNNNGC 2 cut(s) 215, 466
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 4 cut(s) 257, 423, 424, 493
NdeII GATC 2 cut(s) 75, 513
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 5 cut(s) 154, 238, 316, 520, 544
NlaIV GGNNCC 2 cut(s) 266, 279
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 4 cut(s) 127, 198, 304, 487
NspI RCATGY 1 cut(s) 544
PagI TCATGA 2 cut(s) 150, 516
PciI ACATGT 1 cut(s) 540
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 298
PflMI CCANNNNNTGG 1 cut(s) 589
PkrI GCNGC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 278
PscI ACATGT 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 410
Psp5II RGGWCCY 1 cut(s) 278
PspEI GGTNACC 2 cut(s) 127, 487
PspFI CCCAGC 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 266, 279
PspPI GGNCC 1 cut(s) 278
PspPPI RGGWCCY 1 cut(s) 278
RsaI GTAC 2 cut(s) 86, 317
RsaNI GTAC 2 cut(s) 85, 316
RseI CAYNNNNRTG 3 cut(s) 48, 149, 311
SacI GAGCTC 1 cut(s) 410
SalI GTCGAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 2 cut(s) 75, 513
Sau96I GGNCC 1 cut(s) 278
ScrFI CCNGG 4 cut(s) 257, 423, 424, 493
SduI GDGCHC 3 cut(s) 21, 410, 551
SetI ASST 8 cut(s) 195, 283, 311, 325, 364, 374, 410, 450
SfaNI GCATC 2 cut(s) 13, 456
SinI GGWCC 1 cut(s) 278
SmaI CCCGGG 1 cut(s) 424
SmiMI CAYNNNNRTG 3 cut(s) 48, 149, 311
Sse9I AATT 2 cut(s) 384, 586
SsiI CCGC 2 cut(s) 216, 536
SspMI CTAG 1 cut(s) 179
SstI GAGCTC 1 cut(s) 410
StyD4I CCNGG 4 cut(s) 255, 421, 422, 491
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 4 cut(s) 133, 148, 487, 580
TaiI ACGT 2 cut(s) 195, 311
TaqI TCGA 3 cut(s) 231, 291, 374
TasI AATT 2 cut(s) 384, 586
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 218
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 4 cut(s) 127, 198, 304, 487
Tsp45I GTSAC 4 cut(s) 127, 198, 304, 487
TspDTI ATGAA 6 cut(s) 66, 94, 167, 251, 301, 533
TspMI CCCGGG 1 cut(s) 422
TspRI CASTG 1 cut(s) 583
Van91I CCANNNNNTGG 1 cut(s) 589
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 1 cut(s) 586
XceI RCATGY 1 cut(s) 544
XmaI CCCGGG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 374
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.