MD11G1160600.v1.1

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005531
Physical Location & Seq
Reverse (-)
15837955 .. 15839826
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1160600.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGAGATTCCAAGGTCGTCATCAATGGCGCTGCTGCTACTGTCCATCGTCGTCCTCCCAAACTTTTTCTGCGTATGCAGCGCGATCGAGTCGCCTCAGTACTCCGTGGTTCACTCGGAATCCGACTTCGAGATCAGACTCTACAGACTAACCCCCTGGATGTCTGCTCCTGTTAAAGACATCTCCTTCGAGAAAGCCACCAAAAATGGCTTCCACAGGCTGTTCCAGTACATCCAAGGTGCCAATTTGAACTTCTCTCGGATTGCAATGACAGCGCCTGTTTTGACAAGCATAGTCCCAGGAGCTGGGCCCCTTCACTCGTCGGCCTATTTTGTAAGATTCTACGTGCCTACGAAGTTCCAAAGTACCCCTCCCACACCCCTCCCCGAACTCAACTTGAAATCCTATACATGGGATAGTCATTACATTGCCGTCAGGAAGTTCTCTGGGTTCGCAAGAGATACAAACATTGTGAAAGAGGCAGAGAAACTTGCCAGCAGCTTGAGCAGGTCTCCATGGGCAAACTCTACATCAGCAGAAAGTAGCTACGCTTACTCAATTGCACAGTACAACTCCCCATTCCGATTTATTGGTCGTGTGAATGAAGTTTGGGTTGATGTCAATGCATCTGGAGTGGATGGCTATAAATCCAGCGCAATAGCTACGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

224

Amino Acids

24.9

Weight (kDa)

9.02

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 29 - 205 5.4e-53 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 498
AccB1I GGYRCC 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 305
AccII CGCG 1 cut(s) 83
AfaI GTAC 4 cut(s) 101, 230, 367, 569
AfiI CCNNNNNNNGG 2 cut(s) 305, 411
AgsI TTSAA 2 cut(s) 250, 400
AjnI CCWGG 2 cut(s) 155, 298
AjuI GAANNNNNNNTTGG 4 cut(s) 194, 226, 236, 268
AluBI AGCT 4 cut(s) 305, 501, 546, 662
AluI AGCT 4 cut(s) 305, 501, 546, 662
Alw26I GTCTC 1 cut(s) 516
AlwNI CAGNNNCTG 2 cut(s) 278, 305
AoxI GGCC 2 cut(s) 308, 324
ApaI GGGCCC 1 cut(s) 312
ApeKI GCWGC 4 cut(s) 31, 34, 78, 498
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspLEI GCGC 4 cut(s) 31, 83, 277, 656
AspS9I GGNCC 2 cut(s) 308, 309
BaeGI GKGCMC 1 cut(s) 312
BanI GGYRCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 18, 21, 90, 510
BccI CCATC 2 cut(s) 53, 632
BceAI ACGGC 1 cut(s) 416
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 157, 300
BcoDI GTCTC 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 142
BfoI RGCGCY 2 cut(s) 32, 278
BfuAI ACCTGC 1 cut(s) 498
BisI GCNGC 4 cut(s) 32, 35, 79, 499
BlsI GCNGC 4 cut(s) 33, 36, 80, 500
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 2 cut(s) 157, 300
BmgT120I GGNCC 2 cut(s) 308, 309
BmiI GGNNCC 3 cut(s) 241, 310, 311
BmrFI CCNGG 2 cut(s) 157, 300
BmsI GCATC 1 cut(s) 635
BplI GAGNNNNNCTC 2 cut(s) 496, 528
BpmI CTGGAG 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 523
BsaAI YACGTR 2 cut(s) 346, 666
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 6 cut(s) 11, 105, 155, 235, 298, 515
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 411
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 2 cut(s) 273, 426
BseBI CCWGG 2 cut(s) 157, 300
BseDI CCNNGG 6 cut(s) 11, 105, 155, 235, 298, 515
BseGI GGATG 3 cut(s) 165, 231, 643
BseLI CCNNNNNNNGG 2 cut(s) 305, 411
BseMI GCAATG 2 cut(s) 273, 426
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 226
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 4 cut(s) 18, 21, 90, 510
BseYI CCCAGC 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 310, 326
BshNI GGYRCC 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 305, 411
BsmAI GTCTC 1 cut(s) 516
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 2 cut(s) 310, 326
Bso31I GGTCTC 1 cut(s) 516
Bsp120I GGGCCC 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 84, 132
Bsp19I CCATGG 1 cut(s) 515
BspANI GGCC 2 cut(s) 310, 326
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 241, 310, 311
BspMI ACCTGC 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 516
BsrDI GCAATG 2 cut(s) 273, 426
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 6 cut(s) 11, 105, 155, 235, 298, 515
BssMI GATC 2 cut(s) 84, 132
BssT1I CCWWGG 3 cut(s) 11, 235, 515
Bst2UI CCWGG 2 cut(s) 157, 300
Bst4CI ACNGT 2 cut(s) 42, 567
BstBAI YACGTR 2 cut(s) 346, 666
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 2 cut(s) 105, 515
BstF5I GGATG 3 cut(s) 165, 231, 643
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 2 cut(s) 32, 278
BstHHI GCGC 4 cut(s) 31, 83, 277, 656
BstKTI GATC 2 cut(s) 87, 135
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 2 cut(s) 84, 132
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 3 cut(s) 78, 272, 504
BstNI CCWGG 2 cut(s) 157, 300
BstSCI CCNGG 2 cut(s) 155, 298
BstSFI CTRYAG 1 cut(s) 142
BstSLI GKGCMC 1 cut(s) 312
BstSNI TACGTA 1 cut(s) 666
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 4 cut(s) 18, 21, 90, 510
BsuRI GGCC 2 cut(s) 310, 326
BtgI CCRYGG 2 cut(s) 105, 515
BtsCI GGATG 3 cut(s) 165, 231, 643
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 1 cut(s) 496
CaiI CAGNNNCTG 2 cut(s) 278, 305
CfoI GCGC 4 cut(s) 31, 83, 277, 656
Cfr13I GGNCC 2 cut(s) 308, 309
Csp6I GTAC 4 cut(s) 100, 229, 366, 568
CviAII CATG 2 cut(s) 411, 516
CviQI GTAC 4 cut(s) 100, 229, 366, 568
DdeI CTNAG 1 cut(s) 96
DpnI GATC 2 cut(s) 86, 134
DpnII GATC 2 cut(s) 84, 132
Eco105I TACGTA 1 cut(s) 666
Eco130I CCWWGG 3 cut(s) 11, 235, 515
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 155, 298
EcoT14I CCWWGG 3 cut(s) 11, 235, 515
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 3 cut(s) 11, 235, 515
FaeI CATG 2 cut(s) 414, 519
FaiI YATR 6 cut(s) 76, 293, 408, 412, 517, 645
FaqI GGGAC 1 cut(s) 281
FatI CATG 2 cut(s) 410, 515
Fnu4HI GCNGC 4 cut(s) 32, 35, 79, 499
FokI GGATG 3 cut(s) 172, 218, 650
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 4 cut(s) 32, 35, 79, 499
GlaI GCGC 4 cut(s) 30, 82, 276, 655
GluI GCNGC 4 cut(s) 32, 35, 79, 499
GsaI CCCAGC 1 cut(s) 309
GsuI CTGGAG 1 cut(s) 651
HaeII RGCGCY 2 cut(s) 32, 278
HaeIII GGCC 2 cut(s) 310, 326
HhaI GCGC 4 cut(s) 31, 83, 277, 656
Hin1II CATG 2 cut(s) 414, 519
Hin6I GCGC 4 cut(s) 29, 81, 275, 654
HinP1I GCGC 4 cut(s) 29, 81, 275, 654
HinfI GANTC 5 cut(s) 7, 89, 119, 138, 339
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 5 cut(s) 118, 124, 137, 261, 584
Hpy188III TCNNGA 4 cut(s) 130, 190, 436, 630
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 2 cut(s) 53, 325
HpyAV CCTTC 2 cut(s) 196, 323
HpyCH4III ACNGT 2 cut(s) 42, 567
HpyCH4IV ACGT 2 cut(s) 345, 665
HpyCH4V TGCA 4 cut(s) 78, 266, 563, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 272, 504
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 2 cut(s) 345, 665
Hsp92II CATG 2 cut(s) 414, 519
HspAI GCGC 4 cut(s) 29, 81, 275, 654
Kzo9I GATC 2 cut(s) 84, 132
LmnI GCTCC 2 cut(s) 172, 302
Lsp1109I GCAGC 4 cut(s) 18, 21, 90, 510
LweI GCATC 1 cut(s) 635
MaeII ACGT 2 cut(s) 345, 665
MalI GATC 2 cut(s) 86, 134
MboI GATC 2 cut(s) 84, 132
MfeI CAATTG 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 2 cut(s) 244, 558
MlyI GAGTC 2 cut(s) 98, 132
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 5 cut(s) 65, 105, 381, 392, 472
Mph1103I ATGCAT 1 cut(s) 628
MseI TTAA 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 157, 300
MunI CAATTG 1 cut(s) 558
MvaI CCWGG 2 cut(s) 157, 300
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 78, 272, 504
NcoI CCATGG 1 cut(s) 515
NdeII GATC 2 cut(s) 84, 132
NlaIII CATG 2 cut(s) 414, 519
NlaIV GGNNCC 3 cut(s) 241, 310, 311
NsiI ATGCAT 1 cut(s) 628
PfeI GAWTC 3 cut(s) 7, 119, 339
PflMI CCANNNNNTGG 1 cut(s) 305
PkrI GCNGC 4 cut(s) 33, 36, 80, 500
Ple19I CGATCG 1 cut(s) 87
PleI GAGTC 2 cut(s) 97, 132
PpsI GAGTC 2 cut(s) 97, 132
Ppu21I YACGTR 2 cut(s) 346, 666
Psp6I CCWGG 2 cut(s) 155, 298
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 2 cut(s) 155, 298
PspN4I GGNNCC 3 cut(s) 241, 310, 311
PspOMI GGGCCC 1 cut(s) 308
PspPI GGNCC 2 cut(s) 308, 309
PstNI CAGNNNCTG 2 cut(s) 278, 305
PvuI CGATCG 1 cut(s) 87
RsaI GTAC 4 cut(s) 101, 230, 367, 569
RsaNI GTAC 4 cut(s) 100, 229, 366, 568
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 4 cut(s) 32, 35, 79, 499
Sau3AI GATC 2 cut(s) 84, 132
Sau96I GGNCC 2 cut(s) 308, 309
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 2 cut(s) 98, 132
ScrFI CCNGG 2 cut(s) 157, 300
SduI GDGCHC 1 cut(s) 312
SetI ASST 9 cut(s) 17, 241, 307, 348, 503, 512, 548, 664, 668
SfaNI GCATC 1 cut(s) 635
SfcI CTRYAG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
SnaBI TACGTA 1 cut(s) 666
Sse9I AATT 2 cut(s) 244, 558
StyD4I CCNGG 2 cut(s) 155, 298
StyI CCWWGG 3 cut(s) 11, 235, 515
TaaI ACNGT 2 cut(s) 42, 567
TaiI ACGT 2 cut(s) 348, 668
TaqI TCGA 3 cut(s) 87, 129, 189
TasI AATT 2 cut(s) 244, 558
TatI WGTACW 3 cut(s) 99, 228, 567
TfiI GAWTC 3 cut(s) 7, 119, 339
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TseI GCWGC 4 cut(s) 31, 34, 78, 498
TspDTI ATGAA 1 cut(s) 618
TspGWI ACGGA 1 cut(s) 94
Van91I CCANNNNNTGG 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 101
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.