Rw6G000540

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005531
Physical Location & Seq
Forward (+)
919687 .. 932327
12641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000540.1

Sequence Viewer

Length: 549 bp
ATGTGTGTCTCTGTATACCTGTGTGATATTGTATCTGAGGTGGCTGAGATAGAAAAGGTTAAGAAGAGAAGGGAGGAAAGAGCACTTGAAAAAGCTCAACATGCAGAAGAAATGATCAAGGACAAGTTGGGGGAGTTGGCTGAGGAGGCTGTTCCAATACATCCAAGTATCCAAGGTGCCAATTTGAATTGCTCTCGAATTGGCATGACAGCCCCTGTGCTGACAAGAATTGTCCCTGGAGCTGGCCCTCTACACTCATCCGCATACTTTGTGAGATTCTATTTGCGAGTGAAGTTTCAAGCTTCCCCACCCACACCCCTCCCGGAACTCAACTTGAAACCTTATACATGGGACAGTCATTACATTGCGGTCAGAAAATTCTCGGGATTGGCAAGAGATAGTAACATCGTGCAAGAAGCACAGAAACTCGCCACCAGCCTTAGCAGGTCTCCATGGGCTAACTCTACATCAAAAGAAAGTAGATCTGCTTACTCAATTGCACAGTATAACTCCCTTTTCCGGTTGATTGGGCGTGTGAATGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

182

Amino Acids

20.38

Weight (kDa)

9.15

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 50 - 182 1.7e-28 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 15
AciI CCGC 2 cut(s) 261, 368
AcsI RAATTY 1 cut(s) 377
AfiI CCNNNNNNNGG 2 cut(s) 242, 519
AgsI TTSAA 4 cut(s) 89, 187, 299, 337
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 3 cut(s) 95, 242, 302
AluI AGCT 3 cut(s) 95, 242, 302
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 2 cut(s) 13, 453
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 382
AoxI GGCC 1 cut(s) 244
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 382
BanI GGYRCC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 85
BbvCI CCTCAGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 237
BciVI GTATCC 1 cut(s) 179
BclI TGATCA 1 cut(s) 114
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 2 cut(s) 13, 453
BfuAI ACCTGC 1 cut(s) 435
BfuI GTATCC 1 cut(s) 179
BglII AGATCT 1 cut(s) 482
Bme1390I CCNGG 2 cut(s) 237, 323
BmeT110I CYCGRG 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 2 cut(s) 237, 323
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 2 cut(s) 141, 440
BpuMI CCSGG 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 172, 235, 452
BsaWI WCCGGW 1 cut(s) 519
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 519
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 172, 235, 452
BseGI GGATG 2 cut(s) 160, 257
BseLI CCNNNNNNNGG 2 cut(s) 242, 519
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 3 cut(s) 27, 36, 132
BseRI GAGGAG 1 cut(s) 158
BshFI GGCC 1 cut(s) 246
BshNI GGYRCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 382
BsiSI CCGG 2 cut(s) 323, 520
BslFI GGGAC 2 cut(s) 218, 365
BslI CCNNNNNNNGG 2 cut(s) 242, 519
BsmAI GTCTC 2 cut(s) 13, 453
BsmFI GGGAC 2 cut(s) 218, 365
BsnI GGCC 1 cut(s) 246
Bso31I GGTCTC 1 cut(s) 453
BsoBI CYCGRG 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 2 cut(s) 114, 482
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 2 cut(s) 261, 368
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 3 cut(s) 28, 37, 133
BspLI GGNNCC 1 cut(s) 178
BspMI ACCTGC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 363
BssECI CCNNGG 3 cut(s) 172, 235, 452
BssMI GATC 2 cut(s) 114, 482
BssNAI GTATAC 1 cut(s) 16
BssT1I CCWWGG 2 cut(s) 172, 452
Bst1107I GTATAC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 356, 504
Bst6I CTCTTC 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 4 cut(s) 36, 45, 141, 440
BstDSI CCRYGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 160, 257
BstKTI GATC 2 cut(s) 117, 485
BstMAI GTCTC 2 cut(s) 13, 453
BstMBI GATC 2 cut(s) 114, 482
BstMWI GCNNNNNNNGC 2 cut(s) 101, 146
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 235, 321
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BstZ17I GTATAC 1 cut(s) 16
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 452
BtsCI GGATG 2 cut(s) 160, 257
BveI ACCTGC 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 244
CaiI CAGNNNCTG 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 245
CviAII CATG 4 cut(s) 101, 205, 348, 453
DdeI CTNAG 4 cut(s) 36, 45, 141, 440
DpnI GATC 2 cut(s) 116, 484
DpnII GATC 2 cut(s) 114, 482
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 172, 452
Eco31I GGTCTC 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 172, 452
ErhI CCWWGG 2 cut(s) 172, 452
FaeI CATG 4 cut(s) 104, 208, 351, 456
FaiI YATR 8 cut(s) 16, 102, 206, 265, 345, 349, 454, 507
FaqI GGGAC 2 cut(s) 218, 365
FatI CATG 4 cut(s) 100, 204, 347, 452
FbaI TGATCA 1 cut(s) 114
FblI GTMKAC 1 cut(s) 15
FokI GGATG 2 cut(s) 147, 244
GsuI CTGGAG 1 cut(s) 258
HaeIII GGCC 1 cut(s) 246
HapII CCGG 2 cut(s) 323, 520
Hin1II CATG 4 cut(s) 104, 208, 351, 456
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 1 cut(s) 276
HpaII CCGG 2 cut(s) 323, 520
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 2 cut(s) 37, 374
Hpy188III TCNNGA 2 cut(s) 195, 384
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 63
HpyCH4III ACNGT 2 cut(s) 356, 504
HpyCH4V TGCA 3 cut(s) 104, 412, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 146
HpyF3I CTNAG 4 cut(s) 36, 45, 141, 440
Hsp92II CATG 4 cut(s) 104, 208, 351, 456
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 2 cut(s) 114, 482
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 9 cut(s) 32, 222, 228, 228, 249, 336, 430, 448, 533
MaeIII GTNAC 1 cut(s) 401
MalI GATC 2 cut(s) 116, 484
MboI GATC 2 cut(s) 114, 482
MboII GAAGA 2 cut(s) 76, 119
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 6 cut(s) 181, 187, 198, 228, 377, 495
MnlI CCTC 6 cut(s) 31, 67, 136, 139, 258, 329
MseI TTAA 1 cut(s) 60
MspI CCGG 2 cut(s) 323, 520
MspR9I CCNGG 2 cut(s) 237, 323
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 101, 146
NciI CCSGG 1 cut(s) 323
NcoI CCATGG 1 cut(s) 452
NdeII GATC 2 cut(s) 114, 482
NlaIII CATG 4 cut(s) 104, 208, 351, 456
NlaIV GGNNCC 1 cut(s) 178
NspI RCATGY 1 cut(s) 104
PfeI GAWTC 1 cut(s) 276
PfoI TCCNGGA 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 1 cut(s) 482
SaqAI TTAA 1 cut(s) 60
Sau3AI GATC 2 cut(s) 114, 482
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 237, 323
SduI GDGCHC 1 cut(s) 85
SetI ASST 9 cut(s) 21, 42, 60, 97, 178, 244, 304, 343, 449
Sse9I AATT 6 cut(s) 181, 187, 198, 228, 377, 495
SsiI CCGC 2 cut(s) 261, 368
StyD4I CCNGG 2 cut(s) 235, 321
StyI CCWWGG 2 cut(s) 172, 452
TaaI ACNGT 2 cut(s) 356, 504
TaqI TCGA 1 cut(s) 196
TasI AATT 6 cut(s) 181, 187, 198, 228, 377, 495
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.