Rroxscaffold_1G00026340

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005531
Physical Location & Seq
Forward (+)
33185372 .. 33186720
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026340.1

Sequence Viewer

Length: 663 bp
ATGAAGATGGTCAAGAGTGCGATGGTGGTGGTGCTAGTTTTAGTTTTGGTGGTGGTGGTGCCGTGTCTGGAGGCGATTGAATCGCCGCAGTACACGGTGGTGCACTCGGAATCGGAGTTTGAGATCAGAGAATACAGAGTCGGCGCGTGGATGTCTGCTCCTGTTAAAGACATCTCCTTTGAAAAGGCCACCAGAAATGGCTTCCACAGGCTGTTCCAGTACATCCAAGGTGCCAATTTGAATTGCTCTCGAATTGCAATGACGGCTCCTGTGCTGACAAGCATAGTCCCTGGAGCCGGCCCGCTGCACTCATCCGCATACTTTGTGAGTTTCTATTTGCCAGTGAAGTTTCAAGCTTCCCCACCCACACCACTCCCGGAGCTCAACTTGAAACCATATACATGGGACAGTCATTACATTGCAGTCAGGAAATTCTCGGGATTTGCAAGAGATAGTAACATTGTGCAAGAAGCACAGAAACTTGCCACCAGCCTTAGCAGGTCACCATGGGCAAACTCTACATCAGAAGAAAGTAGCTATGCTTACTCGATTGCACAGTATAACTCCCCATTCCGGTGGGTTGGGCGTGTGAATGAAGTTTGGGTCGATGTCAATGGCTCTGGATTAGAGGGAGATAATGGCAGTGAAATAGCTACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

220

Amino Acids

24.45

Weight (kDa)

6.43

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 26 - 202 5.6e-54 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 489
AccB1I GGYRCC 2 cut(s) 58, 230
AccII CGCG 1 cut(s) 146
AciI CCGC 3 cut(s) 86, 302, 315
AcsI RAATTY 1 cut(s) 431
AfaI GTAC 2 cut(s) 92, 221
AfiI CCNNNNNNNGG 2 cut(s) 296, 573
AgsI TTSAA 5 cut(s) 80, 182, 241, 353, 391
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 4 cut(s) 356, 382, 537, 653
AluI AGCT 4 cut(s) 356, 382, 537, 653
Alw21I GWGCWC 2 cut(s) 105, 384
Alw44I GTGCAC 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 2 cut(s) 186, 298
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 304
ApoI RAATTY 1 cut(s) 431
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 299
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 495
AvaI CYCGRG 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 105
BanI GGYRCC 2 cut(s) 58, 230
BanII GRGCYC 1 cut(s) 384
Bbv12I GWGCWC 2 cut(s) 105, 384
BbvI GCAGC 1 cut(s) 291
BccI CCATC 1 cut(s) 16
BceAI ACGGC 2 cut(s) 46, 279
BciT130I CCWGG 1 cut(s) 291
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 1 cut(s) 35
BfuAI ACCTGC 1 cut(s) 489
BisI GCNGC 2 cut(s) 86, 305
BlsI GCNGC 2 cut(s) 87, 306
Bme1390I CCNGG 2 cut(s) 291, 377
BmeT110I CYCGRG 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 4 cut(s) 60, 232, 267, 295
BmrFI CCNGG 2 cut(s) 291, 377
BpmI CTGGAG 2 cut(s) 89, 312
Bpu10I CCTNAGC 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 3 cut(s) 226, 289, 506
BsaWI WCCGGW 1 cut(s) 573
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 573
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 2 cut(s) 217, 341
Bse3DI GCAATG 2 cut(s) 264, 417
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 226, 289, 506
BseGI GGATG 3 cut(s) 156, 222, 311
BseLI CCNNNNNNNGG 2 cut(s) 296, 573
BseMI GCAATG 2 cut(s) 264, 417
BseNI ACTGG 2 cut(s) 217, 341
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 1 cut(s) 291
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 2 cut(s) 188, 300
BshNI GGYRCC 2 cut(s) 58, 230
BsiHKAI GWGCWC 2 cut(s) 105, 384
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 3 cut(s) 297, 377, 574
BslFI GGGAC 2 cut(s) 272, 419
BslI CCNNNNNNNGG 2 cut(s) 296, 573
BsmFI GGGAC 2 cut(s) 272, 419
BsnI GGCC 2 cut(s) 188, 300
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 2 cut(s) 105, 384
Bsp143I GATC 1 cut(s) 123
Bsp19I CCATGG 1 cut(s) 506
BspACI CCGC 3 cut(s) 86, 302, 315
BspANI GGCC 2 cut(s) 188, 300
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 4 cut(s) 60, 232, 267, 295
BspMI ACCTGC 1 cut(s) 489
BspT107I GGYRCC 2 cut(s) 58, 230
BsrDI GCAATG 2 cut(s) 264, 417
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 2 cut(s) 217, 341
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 226, 289, 506
BssMI GATC 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 226, 506
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 97, 410, 558
BstC8I GCNNGC 2 cut(s) 298, 302
BstDEI CTNAG 1 cut(s) 494
BstDSI CCRYGG 1 cut(s) 506
BstEII GGTNACC 1 cut(s) 501
BstF5I GGATG 3 cut(s) 156, 222, 311
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 263
BstNI CCWGG 1 cut(s) 291
BstPI GGTNACC 1 cut(s) 501
BstSCI CCNGG 2 cut(s) 289, 375
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 291
BstXI CCANNNNNNTGG 2 cut(s) 402, 576
BsuRI GGCC 2 cut(s) 188, 300
BtgI CCRYGG 1 cut(s) 506
BtgZI GCGATG 1 cut(s) 35
BtsCI GGATG 3 cut(s) 156, 222, 311
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 2 cut(s) 348, 649
BveI ACCTGC 1 cut(s) 489
Cac8I GCNNGC 2 cut(s) 298, 302
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 299
Csp6I GTAC 2 cut(s) 91, 220
CviAII CATG 2 cut(s) 402, 507
CviQI GTAC 2 cut(s) 91, 220
DdeI CTNAG 1 cut(s) 494
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 226, 506
Eco24I GRGCYC 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 382
EcoO65I GGTNACC 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 226, 506
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 2 cut(s) 226, 506
FaeI CATG 2 cut(s) 405, 510
FaiI YATR 8 cut(s) 284, 319, 397, 399, 403, 508, 540, 561
FaqI GGGAC 2 cut(s) 272, 419
FatI CATG 2 cut(s) 401, 506
FauI CCCGC 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 86, 305
FokI GGATG 3 cut(s) 163, 209, 298
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 2 cut(s) 86, 305
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 145
GluI GCNGC 2 cut(s) 86, 305
GsuI CTGGAG 2 cut(s) 89, 312
HaeIII GGCC 2 cut(s) 188, 300
HapII CCGG 3 cut(s) 297, 377, 574
HhaI GCGC 1 cut(s) 146
Hin1II CATG 2 cut(s) 405, 510
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 3 cut(s) 80, 110, 138
HpaII CCGG 3 cut(s) 297, 377, 574
HphI GGTGA 1 cut(s) 495
Hpy166II GTNNAC 2 cut(s) 93, 103
Hpy188I TCNGA 4 cut(s) 109, 115, 128, 526
Hpy188III TCNNGA 6 cut(s) 13, 68, 249, 427, 438, 621
Hpy8I GTNNAC 2 cut(s) 93, 103
HpyCH4III ACNGT 3 cut(s) 97, 410, 558
HpyCH4V TGCA 7 cut(s) 103, 257, 307, 422, 446, 466, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 263
HpyF3I CTNAG 1 cut(s) 494
Hsp92II CATG 2 cut(s) 405, 510
HspAI GCGC 1 cut(s) 144
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 4 cut(s) 163, 271, 293, 379
Lsp1109I GCAGC 1 cut(s) 291
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 455, 501
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 2 cut(s) 16, 539
MhlI GDGCHC 2 cut(s) 105, 384
MluCI AATT 4 cut(s) 235, 241, 252, 431
MlyI GAGTC 1 cut(s) 147
MnlI CCTC 2 cut(s) 64, 622
MroNI GCCGGC 1 cut(s) 296
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 3 cut(s) 98, 400, 574
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 3 cut(s) 297, 377, 574
MspR9I CCNGG 2 cut(s) 291, 377
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 263
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 506
NdeII GATC 1 cut(s) 123
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 2 cut(s) 405, 510
NlaIV GGNNCC 4 cut(s) 60, 232, 267, 295
NmuCI GTSAC 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 98
PdiI GCCGGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 80, 110
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 2 cut(s) 87, 306
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 289
PspEI GGTNACC 1 cut(s) 501
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 4 cut(s) 60, 232, 267, 295
PspPI GGNCC 1 cut(s) 299
RsaI GTAC 2 cut(s) 92, 221
RsaNI GTAC 2 cut(s) 91, 220
RseI CAYNNNNRTG 3 cut(s) 98, 400, 574
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 2 cut(s) 86, 305
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 299
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 291, 377
SduI GDGCHC 2 cut(s) 105, 384
SetI ASST 7 cut(s) 232, 358, 384, 503, 539, 655, 659
SmiMI CAYNNNNRTG 3 cut(s) 98, 400, 574
Sse9I AATT 4 cut(s) 235, 241, 252, 431
SsiI CCGC 3 cut(s) 86, 302, 315
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 289, 375
StyI CCWWGG 2 cut(s) 226, 506
TaaI ACNGT 3 cut(s) 97, 410, 558
TaqI TCGA 3 cut(s) 250, 548, 606
TasI AATT 4 cut(s) 235, 241, 252, 431
TatI WGTACW 2 cut(s) 90, 219
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 2 cut(s) 80, 110
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 2 cut(s) 348, 649
TseFI GTSAC 1 cut(s) 501
TseI GCWGC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 501
TspDTI ATGAA 2 cut(s) 17, 609
TspRI CASTG 2 cut(s) 348, 649
VneI GTGCAC 1 cut(s) 101
XapI RAATTY 1 cut(s) 431
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.