Rh3BG143900

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005531
Physical Location & Seq
Forward (+)
12217978 .. 12224785
6808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG143900.1

Sequence Viewer

Length: 519 bp
ATGGCTTCCACAGGGAGTGCTTTGACAAATGAAAGGAAACAATTGATACAAATTCGGGCACGGAAAGCAATTAAGAAGCTGCTGTTCCAGTACATCCAAGGTGCTAATTTGAATTGCTCTCGAATTGGCATGACAGCTCCTGTGCTGACAAGCATTGTCCCTGGAGCTGGCCCTCTGCACTCATCCGCATGCTTTGTGAGATTCTATTTGCCAATGAAGTTTCAAGCTTCCCCACCCACACCCCTTCCAGAACTCAACTTGAAACCTTATACATGTGATAGTCATTACACTACGGTCAGGAAATTCTCGGGATTTGCAAGAGATAGTAACATTGTGCAAGAAGCACAGAAACTCGCCACCAGACTTAGCAGGTCTCCATGGGCTAATTCTACATCAGAAGAAAGTAGATCTACTTACTCGATTGCACAGTATAACTCCCCTTTCCGGTTGATTGGGTGTGTGAATGAAGTTTGGGTCGATGTCAATGCCTCTGGATTGCTTTTCGATTCTTTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

172

Amino Acids

19.13

Weight (kDa)

9.66

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 25 - 159 4.5e-33 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 360
AciI CCGC 1 cut(s) 186
AcsI RAATTY 2 cut(s) 51, 302
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 167, 444
AflIII ACRYGT 1 cut(s) 272
AgsI TTSAA 3 cut(s) 112, 224, 262
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 4 cut(s) 79, 137, 167, 227
AluI AGCT 4 cut(s) 79, 137, 167, 227
Alw26I GTCTC 1 cut(s) 378
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 79
ApoI RAATTY 2 cut(s) 51, 302
AspS9I GGNCC 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 61
BbvI GCAGC 1 cut(s) 66
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 1 cut(s) 162
BcoDI GTCTC 1 cut(s) 378
BfuAI ACCTGC 1 cut(s) 360
BglII AGATCT 1 cut(s) 407
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 97, 160, 377
BsaWI WCCGGW 1 cut(s) 444
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 444
Bse1I ACTGG 1 cut(s) 88
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 97, 160, 377
BseGI GGATG 2 cut(s) 93, 182
BseLI CCNNNNNNNGG 2 cut(s) 167, 444
BseNI ACTGG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 445
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 2 cut(s) 167, 444
BsmAI GTCTC 1 cut(s) 378
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 1 cut(s) 171
Bso31I GGTCTC 1 cut(s) 378
BsoBI CYCGRG 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 407
Bsp19I CCATGG 1 cut(s) 377
BspACI CCGC 1 cut(s) 186
BspANI GGCC 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 360
BspTNI GGTCTC 1 cut(s) 378
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 3 cut(s) 97, 160, 377
BssMI GATC 1 cut(s) 407
BssT1I CCWWGG 2 cut(s) 97, 377
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 295, 429
BstC8I GCNNGC 2 cut(s) 169, 190
BstDEI CTNAG 1 cut(s) 365
BstDSI CCRYGG 1 cut(s) 377
BstF5I GGATG 2 cut(s) 93, 182
BstKTI GATC 1 cut(s) 410
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 1 cut(s) 407
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 2 cut(s) 192, 276
BstSCI CCNGG 1 cut(s) 160
BstSLI GKGCMC 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 377
BtsCI GGATG 2 cut(s) 93, 182
BveI ACCTGC 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 169, 190
CaiI CAGNNNCTG 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 91
CviAII CATG 4 cut(s) 130, 189, 273, 378
CviJI RGCY 7 cut(s) 5, 79, 137, 167, 171, 227, 383
CviKI_1 RGCY 7 cut(s) 5, 79, 137, 167, 171, 227, 383
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 365
DpnI GATC 1 cut(s) 409
DpnII GATC 1 cut(s) 407
Eco130I CCWWGG 2 cut(s) 97, 377
Eco31I GGTCTC 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 97, 377
ErhI CCWWGG 2 cut(s) 97, 377
FaeI CATG 4 cut(s) 133, 192, 276, 381
FaiI YATR 6 cut(s) 131, 190, 270, 274, 379, 432
FaqI GGGAC 1 cut(s) 143
FatI CATG 4 cut(s) 129, 188, 272, 377
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 2 cut(s) 80, 169
Fsp4HI GCNGC 1 cut(s) 80
GluI GCNGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 171
HapII CCGG 1 cut(s) 445
Hin1II CATG 4 cut(s) 133, 192, 276, 381
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 2 cut(s) 201, 506
HpaII CCGG 1 cut(s) 445
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 5 cut(s) 120, 248, 298, 309, 492
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 2 cut(s) 295, 429
HpyCH4V TGCA 4 cut(s) 178, 317, 337, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 1 cut(s) 365
Hsp92II CATG 4 cut(s) 133, 192, 276, 381
Kzo9I GATC 1 cut(s) 407
LmnI GCTCC 2 cut(s) 142, 164
Lsp1109I GCAGC 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 326
MalI GATC 1 cut(s) 409
MboI GATC 1 cut(s) 407
MboII GAAGA 1 cut(s) 410
MfeI CAATTG 1 cut(s) 41
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 8 cut(s) 41, 51, 69, 106, 112, 123, 302, 385
MnlI CCTC 2 cut(s) 183, 499
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 187
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 1 cut(s) 162
MunI CAATTG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 65
NcoI CCATGG 1 cut(s) 377
NdeII GATC 1 cut(s) 407
NlaIII CATG 4 cut(s) 133, 192, 276, 381
NspI RCATGY 2 cut(s) 192, 276
PaeI GCATGC 1 cut(s) 192
PciI ACATGT 1 cut(s) 272
PfeI GAWTC 2 cut(s) 201, 506
PkrI GCNGC 1 cut(s) 81
PscI ACATGT 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspPI GGNCC 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 407
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 187
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 1 cut(s) 407
Sau96I GGNCC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 1 cut(s) 61
SetI ASST 7 cut(s) 81, 103, 139, 169, 229, 268, 374
SmiMI CAYNNNNRTG 1 cut(s) 187
SphI GCATGC 1 cut(s) 192
Sse9I AATT 8 cut(s) 41, 51, 69, 106, 112, 123, 302, 385
SsiI CCGC 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 2 cut(s) 97, 377
TaaI ACNGT 2 cut(s) 295, 429
TaqI TCGA 4 cut(s) 121, 419, 477, 504
TasI AATT 8 cut(s) 41, 51, 69, 106, 112, 123, 302, 385
TatI WGTACW 1 cut(s) 90
TfiI GAWTC 2 cut(s) 201, 506
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 3 cut(s) 45, 230, 480
TspGWI ACGGA 1 cut(s) 76
XapI RAATTY 2 cut(s) 51, 302
XceI RCATGY 2 cut(s) 192, 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.