RchiOBHm_Chr5g0054621

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005531
Physical Location & Seq
Reverse (-)
57266652 .. 57268059
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33173

Sequence Viewer

Length: 657 bp
ATGGAGATGGTCAAGAGTGCGATGGCGGTGGTGCTAGTTTTGGTAGCGATGGTGCCGAGTCTGGAGGCGATTGAATCGCCGCAGTACACGGTGGTGCACTGGGAATCGGAGTTTGAGATAAGAGAATACAGAGCCGGCGCGTGGATGTCTGCTCCTGTTAAAGACATCTCCTTTGAAAAGGCCACCAGAAATGGCTTCCACAGGCTGTTCCAGTACATCCAAGGTGCCAATTTGAATTACTCTCGAATTGCAATGACAGCTCCTGTGCTGACGAGCATAGTCCCTGGAGCCGGCCCGCTGCACTCATCCGCATACTTTGTGAGTTTCTATTTGCCAGTGAAGTTTCAAGCTTCCCCACCCACACCACTCCCGGAACTCAACTTGAAACCATATACATGGGACAGTCATTACATTGCAGTCAGGAAATTCTCGGGATTTGCAAGAGATAGTAACATTGTGCAAGAAGCACAGAAACTTGCCACCAGCCTTAGCAAGTCTTCATGGGCAAACTCTACATCAGAAGAAAGTAGCTATGCTTACTCGATTGCACAGTATAACTCCCCATTCCAGTGGGTTGGGCGTGTCAATGAAATTTGGGTCGATGTCAATGCCACTGGATTGGAGGGAGATAATGGCAGTGAAATAGCTACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

218

Amino Acids

24.31

Weight (kDa)

5.58

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 24 - 200 2e-54 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 52, 224
AccII CGCG 1 cut(s) 140
AciI CCGC 4 cut(s) 26, 80, 296, 309
AcsI RAATTY 2 cut(s) 425, 591
AfaI GTAC 2 cut(s) 86, 215
AfiI CCNNNNNNNGG 2 cut(s) 141, 290
AgsI TTSAA 5 cut(s) 74, 176, 235, 347, 385
AjnI CCWGG 1 cut(s) 283
AleI CACNNNNGTG 1 cut(s) 92
AluBI AGCT 4 cut(s) 260, 350, 531, 647
AluI AGCT 4 cut(s) 260, 350, 531, 647
Alw21I GWGCWC 1 cut(s) 99
Alw44I GTGCAC 1 cut(s) 95
AlwNI CAGNNNCTG 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 2 cut(s) 180, 292
ApaLI GTGCAC 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 298
ApoI RAATTY 2 cut(s) 425, 591
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 99
BanI GGYRCC 2 cut(s) 52, 224
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 285
BccI CCATC 2 cut(s) 16, 43
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 1 cut(s) 285
BcnI CCSGG 1 cut(s) 371
BfaI CTAG 1 cut(s) 35
BisI GCNGC 2 cut(s) 80, 299
BlsI GCNGC 2 cut(s) 81, 300
Bme1390I CCNGG 2 cut(s) 285, 371
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 3 cut(s) 54, 226, 289
BmrFI CCNGG 2 cut(s) 285, 371
BmrI ACTGGG 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 109
BpiI GAAGAC 1 cut(s) 489
BpmI CTGGAG 2 cut(s) 83, 306
Bpu10I CCTNAGC 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 220, 283
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 290
Bse118I RCCGGY 2 cut(s) 134, 290
Bse1I ACTGG 5 cut(s) 104, 211, 335, 568, 619
Bse3DI GCAATG 2 cut(s) 258, 411
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 220, 283
BseGI GGATG 3 cut(s) 150, 216, 305
BseLI CCNNNNNNNGG 2 cut(s) 141, 290
BseMI GCAATG 2 cut(s) 258, 411
BseNI ACTGG 5 cut(s) 104, 211, 335, 568, 619
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 2 cut(s) 182, 294
BshNI GGYRCC 2 cut(s) 52, 224
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 3 cut(s) 135, 291, 371
BslFI GGGAC 2 cut(s) 266, 413
BslI CCNNNNNNNGG 2 cut(s) 141, 290
BsmFI GGGAC 2 cut(s) 266, 413
BsnI GGCC 2 cut(s) 182, 294
BsoBI CYCGRG 1 cut(s) 430
Bsp1286I GDGCHC 1 cut(s) 99
BspACI CCGC 4 cut(s) 26, 80, 296, 309
BspANI GGCC 2 cut(s) 182, 294
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 3 cut(s) 54, 226, 289
BspT107I GGYRCC 2 cut(s) 52, 224
BsrDI GCAATG 2 cut(s) 258, 411
BsrFI RCCGGY 2 cut(s) 134, 290
BsrI ACTGG 5 cut(s) 104, 211, 335, 568, 619
BssAI RCCGGY 2 cut(s) 134, 290
BssECI CCNNGG 2 cut(s) 220, 283
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 91, 404, 552
BstC8I GCNNGC 3 cut(s) 136, 292, 296
BstDEI CTNAG 1 cut(s) 488
BstF5I GGATG 3 cut(s) 150, 216, 305
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 140
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNI CCWGG 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 283, 369
BstSLI GKGCMC 1 cut(s) 99
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 489
BstXI CCANNNNNNTGG 4 cut(s) 396, 570, 575, 619
BsuRI GGCC 2 cut(s) 182, 294
BtgZI GCGATG 2 cut(s) 35, 62
BtsCI GGATG 3 cut(s) 150, 216, 305
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 5 cut(s) 97, 342, 575, 612, 643
Cac8I GCNNGC 3 cut(s) 136, 292, 296
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 2 cut(s) 134, 290
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 2 cut(s) 85, 214
CviAII CATG 2 cut(s) 396, 501
CviQI GTAC 2 cut(s) 85, 214
DdeI CTNAG 1 cut(s) 488
Eco130I CCWWGG 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 2 cut(s) 399, 504
FaiI YATR 8 cut(s) 278, 313, 391, 393, 397, 502, 534, 555
FaqI GGGAC 2 cut(s) 266, 413
FatI CATG 2 cut(s) 395, 500
FauI CCCGC 1 cut(s) 303
Fnu4HI GCNGC 2 cut(s) 80, 299
FokI GGATG 3 cut(s) 157, 203, 292
Fsp4HI GCNGC 2 cut(s) 80, 299
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 80, 299
GsuI CTGGAG 2 cut(s) 83, 306
HaeIII GGCC 2 cut(s) 182, 294
HapII CCGG 3 cut(s) 135, 291, 371
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 399, 504
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 3 cut(s) 58, 74, 104
HpaII CCGG 3 cut(s) 135, 291, 371
Hpy166II GTNNAC 2 cut(s) 87, 97
Hpy188I TCNGA 2 cut(s) 109, 520
Hpy188III TCNNGA 5 cut(s) 13, 62, 243, 421, 432
Hpy8I GTNNAC 2 cut(s) 87, 97
HpyCH4III ACNGT 3 cut(s) 91, 404, 552
HpyCH4V TGCA 7 cut(s) 97, 251, 301, 416, 440, 460, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 1 cut(s) 488
Hsp92II CATG 2 cut(s) 399, 504
HspAI GCGC 1 cut(s) 138
KroI GCCGGC 2 cut(s) 134, 290
KroNI GCCGGC 2 cut(s) 136, 292
LmnI GCTCC 3 cut(s) 157, 265, 287
Lsp1109I GCAGC 1 cut(s) 285
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 1 cut(s) 449
MboII GAAGA 2 cut(s) 489, 533
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 5 cut(s) 229, 235, 246, 425, 591
MlyI GAGTC 1 cut(s) 67
MnlI CCTC 2 cut(s) 58, 616
MroNI GCCGGC 2 cut(s) 134, 290
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 92, 394, 568
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 3 cut(s) 135, 291, 371
MspR9I CCNGG 2 cut(s) 285, 371
MvaI CCWGG 1 cut(s) 285
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 257
NaeI GCCGGC 2 cut(s) 136, 292
NciI CCSGG 1 cut(s) 371
NgoMIV GCCGGC 2 cut(s) 134, 290
NlaIII CATG 2 cut(s) 399, 504
NlaIV GGNNCC 3 cut(s) 54, 226, 289
NmeAIII GCCGAG 1 cut(s) 81
OliI CACNNNNGTG 1 cut(s) 92
PdiI GCCGGC 2 cut(s) 136, 292
PfeI GAWTC 2 cut(s) 74, 104
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 2 cut(s) 81, 300
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 3 cut(s) 54, 226, 289
PspPI GGNCC 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 263
RsaI GTAC 2 cut(s) 86, 215
RsaNI GTAC 2 cut(s) 85, 214
RseI CAYNNNNRTG 3 cut(s) 92, 394, 568
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 2 cut(s) 80, 299
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 285, 371
SduI GDGCHC 1 cut(s) 99
SetI ASST 6 cut(s) 226, 262, 352, 533, 649, 653
SmiMI CAYNNNNRTG 3 cut(s) 92, 394, 568
Sse9I AATT 5 cut(s) 229, 235, 246, 425, 591
SsiI CCGC 4 cut(s) 26, 80, 296, 309
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 283, 369
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 3 cut(s) 91, 404, 552
TaqI TCGA 3 cut(s) 244, 542, 600
TasI AATT 5 cut(s) 229, 235, 246, 425, 591
TatI WGTACW 2 cut(s) 84, 213
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 2 cut(s) 74, 104
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 5 cut(s) 104, 342, 575, 619, 643
TseI GCWGC 1 cut(s) 298
TspDTI ATGAA 2 cut(s) 489, 603
TspRI CASTG 5 cut(s) 104, 342, 575, 619, 643
VneI GTGCAC 1 cut(s) 95
XapI RAATTY 2 cut(s) 425, 591
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.