Rw5G033570

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005531
Physical Location & Seq
Reverse (-)
57195094 .. 57196342
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033570.1

Sequence Viewer

Length: 660 bp
ATGGAGATGGTCAAGAGTGCGATGGTGGTGGTGGTGCTAGTTTGGGTGGCGATGGTGCCGAGTCTGGAGGCGATTGAATCGCCACAGTACACGGTGGTGCACTGGGAATCGGAGTTTGAGATAAGAGAATACAGAGCCGGCGCGTGGATGTCTGCTCCTGTTAAAGACATCTCCTTTGAAAAGGCCACCAGAAATGGCTTCCACAGGCTGTTCCAGTACATCCAAGGTGCCAATTTGAATTACTCTCGAATTGCAATGACGGCTCCTGTGCTGACAAGCATAGTCCCTGGAGCCGGCCCGCTGCACTCATCCGCATACTTTGTGCGTTTCTATTTGCCAGTGAAGTTTCAAGCTTCCCCACCCACACCACTCCCGGAACTCAACTTGAAACCATATACATGGGACGGTCATTACATTGCAGTCAGGAAATTCTCGGGATTTGCAAGAGATAGTAACATTGTGCAAGAAGCACAGAAACTCGCCACCAGCCTTAGCAGGTCTTCATGGGCAAACTCTACATCAGAAGAAAGTAGCTATGCTTACTCGATTGCACAGTATAACTCCCCATTCCAGTGGGTTGGGCGTGTGAATGAAATTTGGGTCGATGTCAATGCCACTGGATTAGAGGGAGATAATGGCAGTGAAATAGCTACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

219

Amino Acids

24.58

Weight (kDa)

5.82

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 25 - 201 4.2e-54 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AccB1I GGYRCC 2 cut(s) 55, 227
AccII CGCG 1 cut(s) 143
AciI CCGC 2 cut(s) 299, 312
AcsI RAATTY 2 cut(s) 428, 594
AfaI GTAC 2 cut(s) 89, 218
AfiI CCNNNNNNNGG 2 cut(s) 144, 293
AgsI TTSAA 5 cut(s) 77, 179, 238, 350, 388
AjnI CCWGG 1 cut(s) 286
AleI CACNNNNGTG 1 cut(s) 95
AluBI AGCT 3 cut(s) 353, 534, 650
AluI AGCT 3 cut(s) 353, 534, 650
Alw21I GWGCWC 1 cut(s) 102
Alw44I GTGCAC 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 2 cut(s) 183, 295
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 301
ApoI RAATTY 2 cut(s) 428, 594
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 433
BaeGI GKGCMC 1 cut(s) 102
BanI GGYRCC 2 cut(s) 55, 227
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 288
BccI CCATC 2 cut(s) 16, 46
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 374
BfaI CTAG 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 486
BisI GCNGC 1 cut(s) 302
BlsI GCNGC 1 cut(s) 303
Bme1390I CCNGG 2 cut(s) 288, 374
BmeT110I CYCGRG 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 4 cut(s) 57, 229, 264, 292
BmrFI CCNGG 2 cut(s) 288, 374
BmrI ACTGGG 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 86, 309
Bpu10I CCTNAGC 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 223, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 293
Bse118I RCCGGY 2 cut(s) 137, 293
Bse1I ACTGG 5 cut(s) 107, 214, 338, 571, 622
Bse3DI GCAATG 2 cut(s) 261, 414
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 223, 286
BseGI GGATG 3 cut(s) 153, 219, 308
BseLI CCNNNNNNNGG 2 cut(s) 144, 293
BseMI GCAATG 2 cut(s) 261, 414
BseNI ACTGG 5 cut(s) 107, 214, 338, 571, 622
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 1 cut(s) 288
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 185, 297
BshNI GGYRCC 2 cut(s) 55, 227
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 3 cut(s) 138, 294, 374
BslFI GGGAC 2 cut(s) 269, 416
BslI CCNNNNNNNGG 2 cut(s) 144, 293
BsmFI GGGAC 2 cut(s) 269, 416
BsnI GGCC 2 cut(s) 185, 297
BsoBI CYCGRG 1 cut(s) 433
Bsp1286I GDGCHC 1 cut(s) 102
BspACI CCGC 2 cut(s) 299, 312
BspANI GGCC 2 cut(s) 185, 297
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 4 cut(s) 57, 229, 264, 292
BspMI ACCTGC 1 cut(s) 486
BspT107I GGYRCC 2 cut(s) 55, 227
BsrDI GCAATG 2 cut(s) 261, 414
BsrFI RCCGGY 2 cut(s) 137, 293
BsrI ACTGG 5 cut(s) 107, 214, 338, 571, 622
BssAI RCCGGY 2 cut(s) 137, 293
BssECI CCNNGG 2 cut(s) 223, 286
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 4 cut(s) 87, 94, 407, 555
BstC8I GCNNGC 3 cut(s) 139, 295, 299
BstDEI CTNAG 1 cut(s) 491
BstF5I GGATG 3 cut(s) 153, 219, 308
BstFNI CGCG 1 cut(s) 143
BstHHI GCGC 1 cut(s) 143
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 286, 372
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 492
BstXI CCANNNNNNTGG 3 cut(s) 399, 573, 578
BsuRI GGCC 2 cut(s) 185, 297
BtgZI GCGATG 2 cut(s) 35, 65
BtsCI GGATG 3 cut(s) 153, 219, 308
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 5 cut(s) 100, 345, 578, 615, 646
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 3 cut(s) 139, 295, 299
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 2 cut(s) 137, 293
Cfr13I GGNCC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 88, 217
CviAII CATG 2 cut(s) 399, 504
CviQI GTAC 2 cut(s) 88, 217
DdeI CTNAG 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 2 cut(s) 402, 507
FaiI YATR 8 cut(s) 281, 316, 394, 396, 400, 505, 537, 558
FaqI GGGAC 2 cut(s) 269, 416
FatI CATG 2 cut(s) 398, 503
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 1 cut(s) 302
FokI GGATG 3 cut(s) 160, 206, 295
Fsp4HI GCNGC 1 cut(s) 302
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 142
GluI GCNGC 1 cut(s) 302
GsuI CTGGAG 2 cut(s) 86, 309
HaeIII GGCC 2 cut(s) 185, 297
HapII CCGG 3 cut(s) 138, 294, 374
HhaI GCGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 402, 507
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 3 cut(s) 61, 77, 107
HpaII CCGG 3 cut(s) 138, 294, 374
Hpy166II GTNNAC 2 cut(s) 90, 100
Hpy188I TCNGA 2 cut(s) 112, 523
Hpy188III TCNNGA 5 cut(s) 13, 65, 246, 424, 435
Hpy8I GTNNAC 2 cut(s) 90, 100
HpyCH4III ACNGT 4 cut(s) 87, 94, 407, 555
HpyCH4V TGCA 7 cut(s) 100, 254, 304, 419, 443, 463, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 1 cut(s) 491
Hsp92II CATG 2 cut(s) 402, 507
HspAI GCGC 1 cut(s) 141
KroI GCCGGC 2 cut(s) 137, 293
KroNI GCCGGC 2 cut(s) 139, 295
LmnI GCTCC 3 cut(s) 160, 268, 290
Lsp1109I GCAGC 1 cut(s) 288
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 452
MboII GAAGA 2 cut(s) 492, 536
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 5 cut(s) 232, 238, 249, 428, 594
MlyI GAGTC 1 cut(s) 70
MnlI CCTC 2 cut(s) 61, 619
MroNI GCCGGC 2 cut(s) 137, 293
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 3 cut(s) 95, 397, 571
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 3 cut(s) 138, 294, 374
MspR9I CCNGG 2 cut(s) 288, 374
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 260
NaeI GCCGGC 2 cut(s) 139, 295
NciI CCSGG 1 cut(s) 374
NgoMIV GCCGGC 2 cut(s) 137, 293
NlaIII CATG 2 cut(s) 402, 507
NlaIV GGNNCC 4 cut(s) 57, 229, 264, 292
NmeAIII GCCGAG 1 cut(s) 84
OliI CACNNNNGTG 1 cut(s) 95
PdiI GCCGGC 2 cut(s) 139, 295
PfeI GAWTC 2 cut(s) 77, 107
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 1 cut(s) 303
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 4 cut(s) 57, 229, 264, 292
PspPI GGNCC 1 cut(s) 296
RsaI GTAC 2 cut(s) 89, 218
RsaNI GTAC 2 cut(s) 88, 217
RseI CAYNNNNRTG 3 cut(s) 95, 397, 571
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 1 cut(s) 302
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 288, 374
SduI GDGCHC 1 cut(s) 102
SetI ASST 6 cut(s) 229, 355, 500, 536, 652, 656
SmiMI CAYNNNNRTG 3 cut(s) 95, 397, 571
Sse9I AATT 5 cut(s) 232, 238, 249, 428, 594
SsiI CCGC 2 cut(s) 299, 312
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 286, 372
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 4 cut(s) 87, 94, 407, 555
TaqI TCGA 3 cut(s) 247, 545, 603
TasI AATT 5 cut(s) 232, 238, 249, 428, 594
TatI WGTACW 2 cut(s) 87, 216
TfiI GAWTC 2 cut(s) 77, 107
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 5 cut(s) 107, 345, 578, 622, 646
TseI GCWGC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 492, 606
TspRI CASTG 5 cut(s) 107, 345, 578, 622, 646
VneI GTGCAC 1 cut(s) 98
XapI RAATTY 2 cut(s) 428, 594
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.