RLG00000014360

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005531
Physical Location & Seq
Forward (+)
52038339 .. 52039713
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014360

Sequence Viewer

Length: 558 bp
ATGCTCAGCTCGAATCTGGCTTCAATGGAAGTCGACAAGCAGACCCTGCAATCATATTCGGTTAGAGCTGCTCTAAAGGAGGTGTTTAATTATATGGTGCAAAAGTCAGTTCTTCAAACTAGTGAGAGAAACTTACAACATATATTGGATCAATATTACACTTACAGGCTGTTCCAGTACATCCAAGGTGCCAATTTGAATTGCTCTCGAATTGGCATGACTGCTCCTGTGCTGACAAGCATTGTCCTTAGAGCTGGCCCTCTGCACTCATCCGCATACTTTGTGAGAGTCTATTTGCCAGTGAAGTTTCAAGCTTCCCCACCCACACCTCTTCCGGAACTCAACTTGAAACCTTATACATGGGACAGTCATTACATTGCGGTTAGGAAATTCTCGGGATTTGCAAGAGATAGTAACATTGTGCAAGAAGCACAGAAACTCGCCACTAGCCTTAGCAGGTCGCCATGGGCTAACTCTACATCAGAAGAAAGTAGTTCTGCTGACTTGATTGCACAGTATAACTCCCCGGCCTTTCCGGTTGATTGGGTGTGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

186

Amino Acids

20.9

Weight (kDa)

8.5

Isoelectric Point (pI)

53.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 44 - 178 2.1e-28 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 188
AccI GTMKAC 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 2 cut(s) 273, 380
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 1 cut(s) 389
AfaI GTAC 1 cut(s) 179
AgsI TTSAA 5 cut(s) 24, 116, 199, 311, 349
AhlI ACTAGT 1 cut(s) 119
AluBI AGCT 4 cut(s) 9, 68, 254, 314
AluI AGCT 4 cut(s) 9, 68, 254, 314
AlwI GGATC 1 cut(s) 156
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 394
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 2 cut(s) 256, 528
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 257
AsuC2I CCSGG 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 394
BanI GGYRCC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 55
BcnI CCSGG 1 cut(s) 527
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 2 cut(s) 120, 447
BfuAI ACCTGC 1 cut(s) 447
BisI GCNGC 1 cut(s) 69
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 452
Bpu1102I GCTNAGC 1 cut(s) 5
BpuMI CCSGG 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 184, 464, 525
BsaWI WCCGGW 2 cut(s) 334, 535
Bse1I ACTGG 2 cut(s) 175, 299
Bse3DI GCAATG 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 334
BseDI CCNNGG 3 cut(s) 184, 464, 525
BseGI GGATG 2 cut(s) 180, 269
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 19
BseNI ACTGG 2 cut(s) 175, 299
BseXI GCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 248
BshFI GGCC 2 cut(s) 258, 530
BshNI GGYRCC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 3 cut(s) 335, 527, 536
BslFI GGGAC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 258, 530
BsoBI CYCGRG 1 cut(s) 394
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 1 cut(s) 148
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 2 cut(s) 273, 380
BspANI GGCC 2 cut(s) 258, 530
BspCNI CTCAG 1 cut(s) 18
BspEI TCCGGA 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 447
BspPI GGATC 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 2 cut(s) 175, 299
BssECI CCNNGG 3 cut(s) 184, 464, 525
BssMI GATC 1 cut(s) 148
BssT1I CCWWGG 2 cut(s) 184, 464
Bst4CI ACNGT 2 cut(s) 368, 516
Bst6I CTCTTC 1 cut(s) 336
BstAPI GCANNNNNTGC 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 3 cut(s) 5, 248, 452
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 180, 269
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 525
BstV1I GCAGC 1 cut(s) 55
BsuRI GGCC 2 cut(s) 258, 530
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 180, 269
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 178
CviAII CATG 3 cut(s) 217, 360, 465
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 3 cut(s) 5, 248, 452
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 184, 464
Eco88I CYCGRG 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 184, 464
ErhI CCWWGG 2 cut(s) 184, 464
FaeI CATG 3 cut(s) 220, 363, 468
FaqI GGGAC 1 cut(s) 377
FatI CATG 3 cut(s) 216, 359, 464
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 2 cut(s) 167, 256
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 2 cut(s) 120, 447
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 2 cut(s) 258, 530
HapII CCGG 3 cut(s) 335, 527, 536
Hin1II CATG 3 cut(s) 220, 363, 468
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 2 cut(s) 13, 288
HpaII CCGG 3 cut(s) 335, 527, 536
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 1 cut(s) 484
Hpy188III TCNNGA 3 cut(s) 207, 335, 396
Hpy8I GTNNAC 1 cut(s) 34
HpyCH4III ACNGT 2 cut(s) 368, 516
HpyCH4V TGCA 6 cut(s) 49, 100, 265, 404, 424, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 3 cut(s) 5, 248, 452
Hsp92II CATG 3 cut(s) 220, 363, 468
Kpn2I TCCGGA 1 cut(s) 334
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 55
MaeI CTAG 2 cut(s) 120, 447
MaeIII GTNAC 1 cut(s) 413
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 3 cut(s) 104, 323, 497
MluCI AATT 5 cut(s) 88, 193, 199, 210, 389
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 3 cut(s) 73, 270, 339
MroI TCCGGA 1 cut(s) 334
MseI TTAA 1 cut(s) 87
MspI CCGG 3 cut(s) 335, 527, 536
MspR9I CCNGG 1 cut(s) 527
MwoI GCNNNNNNNGC 1 cut(s) 46
NciI CCSGG 1 cut(s) 527
NcoI CCATGG 1 cut(s) 464
NdeII GATC 1 cut(s) 148
NlaIII CATG 3 cut(s) 220, 363, 468
NlaIV GGNNCC 1 cut(s) 190
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 257
PstNI CAGNNNCTG 1 cut(s) 46
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 1 cut(s) 527
SetI ASST 9 cut(s) 11, 70, 84, 190, 256, 316, 331, 355, 461
SpeI ACTAGT 1 cut(s) 119
Sse9I AATT 5 cut(s) 88, 193, 199, 210, 389
SsiI CCGC 2 cut(s) 273, 380
SspI AATATT 1 cut(s) 155
SspMI CTAG 2 cut(s) 120, 447
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 2 cut(s) 184, 464
TaaI ACNGT 2 cut(s) 368, 516
TaqI TCGA 3 cut(s) 11, 33, 208
TasI AATT 5 cut(s) 88, 193, 199, 210, 389
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 306
TseI GCWGC 1 cut(s) 68
TspRI CASTG 1 cut(s) 306
XapI RAATTY 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 2 cut(s) 120, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.