MD12G1031300.v1.1

mRNA-decapping enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002391
Physical Location & Seq
Forward (+)
3454945 .. 3459718
4774 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1031300.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGTCTCAGACGGGGAAATTGATGCCGAATCTCGATCAGCAGAGCACCAAAATGCTCAATCTCACGGTGCTGCAGAGAATCGACCCCTTCATCGAAGAGATTCTGATTACGGCTGCCCACGTTACCTTCTACGAATTCAACATCGAGAGCAATCAATGGAGTCGCAAGGATGTTGAAGGGTCTCTCTTTGTTGTTAAAAGAAATACTCAACCGCGGTTCCAGTTCATCGTTATGAATCGGCGCAATACAGATAACCTAGTGGAGAATCTCTTGGGAGATTTTGAGTATGAAGTTCAAGTTCCATATTTATTGTATAGAAATGCTGCCCAAGAAGTTAATGGCATTTGGTTTTATAATGCTCGTGAATGTGAGGAAGTTGCGAATCTCTTTACTAGGATCCTGAATGCATACTCAAAGGCTCCTCCCAAGTCCAAAATATCTTCAACCAAAAGTGAGTTTGAAGAACTTGAAGCAGTCCCATCCATGTTGGTCATGGAAGGGCCGTTGGAGCCATCATTGAATGCCCCTGCTGTCACTGATGCACCTGAAGATTCTTCCTTTATAAACTACCTGGCAGCAATGAATATTGGAAATAATGCTTCAGATGCCACAAATTCAAGACTGCCCTATCAGTCTACTGGAACTGTTCCTGCTCCTTCTTATGCACCCAGTGCTGTTTCACCTCCTGCACCAACTCCACAAATACCACCACCTTCCCATTCAGTCCCATCTACGCCAACCCCCCTACATGGCTCCTCTGACCAAACCACCAGCAACAATCGGGTCGCTAATCTTATAAAGCCCTCTTCTTTCTTCCCTCTCCCTGCTTCCTCGCCTCCTTTGGTCATACCACCTACCTCTTCATCTGTGCCATCTGCTGCTGCACTTCATCCACCCCTGAACCTGCAACGCCCTTATGGCACCCCAATGCTTCAACCCTTTCCACCACCAACGCCCCCACCATCTTTAACACCCAATTCTGCTCCTCCTGCTCCCAGTGACGGGCCTCTTGTTAGCAGAGAAAAAGTGCGAGAAGCACTTGTAATGCTTGTTCAGGACGATCAATTCATCGACATGATATACCAAGCATTGCTGAAAGTGCACCATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DCP1

Protein Analysis

371

Amino Acids

40.63

Weight (kDa)

5.22

Isoelectric Point (pI)

64.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCP1 PF06058 17 - 131 3e-46 Dcp1-like decapping family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 354, 563, 797
Acc36I ACCTGC 1 cut(s) 912
AccB1I GGYRCC 1 cut(s) 920
AccI GTMKAC 1 cut(s) 635
AccII CGCG 1 cut(s) 214
AciI CCGC 2 cut(s) 212, 214
AclWI GGATC 2 cut(s) 391, 404
AcsI RAATTY 2 cut(s) 134, 613
AcuI CTGAAG 2 cut(s) 567, 585
AdeI CACNNNGTG 1 cut(s) 671
AfiI CCNNNNNNNGG 3 cut(s) 749, 1001, 1002
AgsI TTSAA 9 cut(s) 139, 176, 296, 444, 461, 470, 520, 618, 935
AjnI CCWGG 1 cut(s) 570
Alw21I GWGCWC 2 cut(s) 47, 1104
Alw26I GTCTC 2 cut(s) 9, 186
Alw44I GTGCAC 1 cut(s) 1100
AlwI GGATC 2 cut(s) 391, 404
AoxI GGCC 2 cut(s) 500, 1004
ApaLI GTGCAC 1 cut(s) 1100
ApeKI GCWGC 6 cut(s) 70, 113, 323, 575, 878, 881
ApoI RAATTY 2 cut(s) 134, 613
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 500, 1004
AsuHPI GGTGA 1 cut(s) 672
BaeGI GKGCMC 1 cut(s) 1104
BamHI GGATCC 1 cut(s) 396
BanI GGYRCC 1 cut(s) 920
BauI CACGAG 1 cut(s) 360
Bbv12I GWGCWC 2 cut(s) 47, 1104
BbvI GCAGC 6 cut(s) 57, 100, 310, 587, 865, 868
BccI CCATC 5 cut(s) 487, 520, 736, 880, 970
BceAI ACGGC 2 cut(s) 126, 487
BciT130I CCWGG 1 cut(s) 572
BcoDI GTCTC 2 cut(s) 9, 186
BfaI CTAG 2 cut(s) 257, 393
BfmI CTRYAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 912
BglI GCCNNNNNGGC 1 cut(s) 918
BisI GCNGC 6 cut(s) 71, 114, 324, 576, 879, 882
BlsI GCNGC 6 cut(s) 72, 115, 325, 577, 880, 883
Bme1390I CCNGG 1 cut(s) 572
BmgT120I GGNCC 2 cut(s) 500, 1004
BmiI GGNNCC 6 cut(s) 218, 398, 420, 510, 754, 922
BmrFI CCNGG 1 cut(s) 572
BmrI ACTGGG 2 cut(s) 663, 990
BmsI GCATC 3 cut(s) 12, 529, 595
BmuI ACTGGG 2 cut(s) 663, 990
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 212
Bsc4I CCNNNNNNNGG 3 cut(s) 749, 1001, 1002
Bse1I ACTGG 4 cut(s) 220, 643, 669, 996
Bse3DI GCAATG 2 cut(s) 585, 1088
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 1 cut(s) 212
BseGI GGATG 3 cut(s) 175, 479, 889
BseLI CCNNNNNNNGG 3 cut(s) 749, 1001, 1002
BseMI GCAATG 2 cut(s) 585, 1088
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 4 cut(s) 220, 643, 669, 996
BseRI GAGGAG 3 cut(s) 411, 745, 975
BseSI GKGCMC 1 cut(s) 1104
BseXI GCAGC 6 cut(s) 57, 100, 310, 587, 865, 868
BsgI GTGCAG 2 cut(s) 672, 867
Bsh1236I CGCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 502, 1006
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 2 cut(s) 47, 1104
BslFI GGGAC 2 cut(s) 461, 710
BslI CCNNNNNNNGG 3 cut(s) 749, 1001, 1002
BsmAI GTCTC 2 cut(s) 9, 186
BsmFI GGGAC 2 cut(s) 461, 710
BsmI GAATGC 2 cut(s) 409, 526
BsnI GGCC 2 cut(s) 502, 1006
Bso31I GGTCTC 1 cut(s) 186
Bsp1286I GDGCHC 2 cut(s) 47, 1104
Bsp143I GATC 3 cut(s) 34, 396, 1060
BspACI CCGC 2 cut(s) 212, 214
BspANI GGCC 2 cut(s) 502, 1006
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 6 cut(s) 218, 398, 420, 510, 754, 922
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 912
BspPI GGATC 2 cut(s) 391, 404
BspT107I GGYRCC 1 cut(s) 920
BspTNI GGTCTC 1 cut(s) 186
BsrDI GCAATG 2 cut(s) 585, 1088
BsrI ACTGG 4 cut(s) 220, 643, 669, 996
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 3 cut(s) 34, 396, 1060
BssSI CACGAG 1 cut(s) 360
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 2 cut(s) 67, 646
Bst6I CTCTTC 3 cut(s) 90, 811, 865
BstAPI GCANNNNNTGC 1 cut(s) 671
BstDEI CTNAG 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 3 cut(s) 175, 479, 889
BstFNI CGCG 1 cut(s) 214
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 3 cut(s) 37, 399, 1063
BstMAI GTCTC 2 cut(s) 9, 186
BstMBI GATC 3 cut(s) 34, 396, 1060
BstMWI GCNNNNNNNGC 6 cut(s) 508, 605, 671, 918, 989, 1099
BstNI CCWGG 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 570
BstSFI CTRYAG 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 1104
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 6 cut(s) 57, 100, 310, 587, 865, 868
BstX2I RGATCY 1 cut(s) 396
BstYI RGATCY 1 cut(s) 396
BsuRI GGCC 2 cut(s) 502, 1006
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 3 cut(s) 175, 479, 889
BtsIMutI CAGTG 3 cut(s) 534, 676, 1003
BveI ACCTGC 1 cut(s) 912
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 500, 1004
Cfr42I CCGCGG 1 cut(s) 215
CviAII CATG 5 cut(s) 484, 493, 749, 1075, 1110
CviJI RGCY 7 cut(s) 113, 419, 502, 511, 753, 802, 1006
CviKI_1 RGCY 7 cut(s) 113, 419, 502, 511, 753, 802, 1006
DdeI CTNAG 1 cut(s) 6
DpnI GATC 3 cut(s) 36, 398, 1062
DpnII GATC 3 cut(s) 34, 396, 1060
DraIII CACNNNGTG 1 cut(s) 671
Eam1104I CTCTTC 3 cut(s) 90, 811, 865
EarI CTCTTC 3 cut(s) 90, 811, 865
Eco31I GGTCTC 1 cut(s) 186
Eco57I CTGAAG 2 cut(s) 567, 585
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 409
FaeI CATG 5 cut(s) 487, 496, 752, 1078, 1113
FaqI GGGAC 2 cut(s) 461, 710
FatI CATG 5 cut(s) 483, 492, 748, 1074, 1109
FblI GTMKAC 1 cut(s) 635
Fnu4HI GCNGC 6 cut(s) 71, 114, 324, 576, 879, 882
FokI GGATG 3 cut(s) 182, 466, 876
Fsp4HI GCNGC 6 cut(s) 71, 114, 324, 576, 879, 882
FspBI CTAG 2 cut(s) 257, 393
GlaI GCGC 1 cut(s) 242
GluI GCNGC 6 cut(s) 71, 114, 324, 576, 879, 882
HaeIII GGCC 2 cut(s) 502, 1006
HhaI GCGC 1 cut(s) 243
Hin1II CATG 5 cut(s) 487, 496, 752, 1078, 1113
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 8 cut(s) 28, 78, 100, 160, 235, 265, 382, 551
HphI GGTGA 1 cut(s) 672
Hpy166II GTNNAC 2 cut(s) 636, 1102
Hpy188I TCNGA 4 cut(s) 9, 105, 604, 760
Hpy188III TCNNGA 6 cut(s) 32, 145, 362, 400, 618, 1055
Hpy8I GTNNAC 2 cut(s) 636, 1102
HpyAV CCTTC 6 cut(s) 97, 136, 170, 491, 666, 723
HpyCH4III ACNGT 2 cut(s) 67, 646
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 8 cut(s) 73, 407, 542, 665, 689, 884, 907, 1102
HpyF10VI GCNNNNNNNGC 6 cut(s) 508, 605, 671, 918, 989, 1099
HpyF3I CTNAG 1 cut(s) 6
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 5 cut(s) 487, 496, 752, 1078, 1113
HspAI GCGC 1 cut(s) 241
KspI CCGCGG 1 cut(s) 215
Kzo9I GATC 3 cut(s) 34, 396, 1060
LmnI GCTCC 6 cut(s) 424, 508, 658, 758, 988, 997
Lsp1109I GCAGC 6 cut(s) 57, 100, 310, 587, 865, 868
LweI GCATC 3 cut(s) 12, 529, 595
MaeI CTAG 2 cut(s) 257, 393
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 121, 532, 998
MalI GATC 3 cut(s) 36, 398, 1062
MboI GATC 3 cut(s) 34, 396, 1060
MboII GAAGA 8 cut(s) 107, 432, 473, 546, 560, 798, 805, 852
MflI RGATCY 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 47, 1104
MluCI AATT 5 cut(s) 17, 134, 613, 976, 1064
MlyI GAGTC 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 486
Mph1103I ATGCAT 1 cut(s) 409
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 3 cut(s) 195, 336, 968
MslI CAYNNNNRTG 4 cut(s) 50, 230, 926, 1073
MspA1I CMGCKG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 572
Mva1269I GAATGC 2 cut(s) 409, 526
MvaI CCWGG 1 cut(s) 572
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 6 cut(s) 508, 605, 671, 918, 989, 1099
NdeII GATC 3 cut(s) 34, 396, 1060
NlaIII CATG 5 cut(s) 487, 496, 752, 1078, 1113
NlaIV GGNNCC 6 cut(s) 218, 398, 420, 510, 754, 922
NmuCI GTSAC 2 cut(s) 532, 998
NsiI ATGCAT 1 cut(s) 409
PctI GAATGC 2 cut(s) 409, 526
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 7 cut(s) 28, 78, 100, 235, 265, 382, 551
PkrI GCNGC 6 cut(s) 72, 115, 325, 577, 880, 883
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PsiI TTATAA 3 cut(s) 354, 563, 797
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 6 cut(s) 218, 398, 420, 510, 754, 922
PspPI GGNCC 2 cut(s) 500, 1004
PsrI GAACNNNNNNTAC 2 cut(s) 1035, 1067
PstI CTGCAG 1 cut(s) 75
PsuI RGATCY 1 cut(s) 396
RseI CAYNNNNRTG 4 cut(s) 50, 230, 926, 1073
SacII CCGCGG 1 cut(s) 215
SaqAI TTAA 3 cut(s) 195, 336, 968
SatI GCNGC 6 cut(s) 71, 114, 324, 576, 879, 882
Sau3AI GATC 3 cut(s) 34, 396, 1060
Sau96I GGNCC 2 cut(s) 500, 1004
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 1 cut(s) 572
SduI GDGCHC 2 cut(s) 47, 1104
SfaNI GCATC 3 cut(s) 12, 529, 595
SfcI CTRYAG 1 cut(s) 71
Sfr303I CCGCGG 1 cut(s) 215
SgrBI CCGCGG 1 cut(s) 215
SmiMI CAYNNNNRTG 4 cut(s) 50, 230, 926, 1073
Sse9I AATT 5 cut(s) 17, 134, 613, 976, 1064
SsiI CCGC 2 cut(s) 212, 214
SspI AATATT 1 cut(s) 586
SspMI CTAG 2 cut(s) 257, 393
StyD4I CCNGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 67, 646
TaiI ACGT 1 cut(s) 123
TaqI TCGA 5 cut(s) 33, 81, 93, 144, 1071
TasI AATT 5 cut(s) 17, 134, 613, 976, 1064
TfiI GAWTC 7 cut(s) 28, 78, 100, 235, 265, 382, 551
Tru1I TTAA 3 cut(s) 195, 336, 968
Tru9I TTAA 3 cut(s) 195, 336, 968
TscAI CASTG 3 cut(s) 541, 676, 1003
TseFI GTSAC 2 cut(s) 532, 998
TseI GCWGC 6 cut(s) 70, 113, 323, 575, 878, 881
Tsp45I GTSAC 2 cut(s) 532, 998
TspDTI ATGAA 8 cut(s) 79, 214, 248, 303, 596, 852, 878, 1057
TspRI CASTG 3 cut(s) 541, 676, 1003
VneI GTGCAC 1 cut(s) 1100
XapI RAATTY 2 cut(s) 134, 613
XcmI CCANNNNNNNNNTGG 2 cut(s) 335, 490
XmiI GTMKAC 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 2 cut(s) 257, 393
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.