Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001742

Physical Location & Seq

Genomic Coordinates Reverse (-)

3683415 .. 3690785

Gene Structure 7371 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1033300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGCCCGCTCCTCCTCCATCAACGTCGTCGTCAAGGACGGCGGCCTCAAACTCATCCCAAGTCTCCGACGACGGCCACAGCATACGCTATGAGGACCTGCCGATCCTGTGTGAGCGGCACACGACGTCGAAACTCTCGGCGTTTGAGGACTTGCAGTCGATAAAGTCGATGGGGTTCAGAGGAGAAGCACTTGCCAGCATGACGTACGTAGCTCACGTCACCGTCACCACCATTACCAAGGGCCAGTTGCATGGTTATAGGGTATCTTATAAAGATGGTGTAATGGAGCACGAACCGAAGGCGTGCGCTGCTGTAAAAGGAACTCAAATAACGTGGTGGTACTTGTTATCAGATTTTGCTGATGATTATTCAAAGATTGTAGATTTGCTAAGCCGGTTTGCAATTCATCATATGAATGTTAGCTTCTCCTGCAGAAAGCATGGAGCTGCTAGAGCAGATGTTAATTCTGTTTCCACGGCATCAAGGATTGATGCAATTCGATCGGTGTATGGGGTGTCTGTTGCTCGCAGTCTGATGACAATAGAAGCTTCAGATAAAGATCCTTCTAGCTCCGTTTTTCAGATGGATGGCTTTATCTCCGATTCAAACTATGTTGCCAAGAAGATAACCATGGTGCTTTATATAAATGATAGATTGGTTGATTGCACCGCTCTGAAGAGAGCTCTAGAAGTTGTTTATGCTGCCACATTGCCAAAAGCATCAAAACCCTTCATATACATGTCAATTATGTTACCACCTGAACATGTTGATGTGAATGTTCACCCGACAAAGAGAGAGGTAAGCCTTCTGAATCAGGAAATCATCATTGAGAAGATACAGTCAGTGGTTGAATGGAGGTTGAGAAGCTCCAATGAGACACAAACATTTCAGGAACAGGCAGTCGAACCTTCTTCATCCAGCCAAATGGTTTCAAGAAAGGATTCAAATCAGAACCCCTCACCATCTGCATCAAAATTACACAAGGTTCCAGTGAATAAAATGGTTAGAACGGATTCATCAAATCCTGCTGGTAGGTTGCATGTGTATTTGCAACCAGATCCTCGTGGCCATCTTGAAAGGAATGCTAGCTTGACAGCTGTTAGATCTTCTGTTAGACTAAGACGAAACCCAAAGGAAACCGCTGATCTGACCAGCCTTCAGGAGCTTATTGATGAAATTGACAGCAATTGTCATTCTGGTCTACTGGACATTGTGAGACACTGCACATACATTGGAATGGCAGATGATGTTTTTGCACTGCTTCAGCATGATACCCATCTTTATCTTGCAAATGTAGTGAACTTGAGCAAAGAGCTCATGTATCAGCAAGTCCTGCGTCGGTTTGCCCATTTTAATGCTATACAAATAAGTGAGCCAGCCCCACTGAAGGAGTTGATTGTGTTGGCACTAAAAGAGGAGGAGGATCCAGAATGCAGTGTGAATCATGAACTTAACGAGAAGATTGCAGAGATGAATACAGAACTACTCAAGCAAAAGGCTGCTATGTTGGGGGAGTATTTTTCCATTCATATAGATAATGATGGAAATTTGTCTAGGCTTCCAGTCATACTTGATCAATACACACCTGACATGGATCGTGTCCCTGAACTTGTGCTTTGTTTGGGCAACGATGTTGATTGGGAGGAGGAAAAAAAATGCCTTCAAGTAATTTCAGCTGCACTTGGAAATTTCTATGCCATGCATCCTCCTATGTTGCCCAACCCATCTGGTGATGGCTTGCAATTTTACCAAAAGAGAAAGCCTTTTAGGAATCCTGAAGAAAGTACCTCATGCAATACTGGAGATGACGACGTCATGACAGAAAATGAAATTGAGCACGAACTAGTTGCAGAGGCAGAAAATGCATGGGCTCAGCGCGAATGGTCAATCCAGCATGTTTTGTTTCCATCAATGAGGCTCTTTTTCAAGCCACCAAATTCTATGGCTACAAACGGAACATTTGTACGGGTAGCTTCACTGGAGAAACTATACCGGATTTTCGAAAGATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000793 GO:0000794 GO:0000795 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005712 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009791 GO:0009845 GO:0009987 GO:0010154 GO:0016445 GO:0016446 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0031974 GO:0031981 GO:0032300 GO:0032389 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0061458 GO:0070013 GO:0071704 GO:0090304 GO:0090351 GO:0097159 GO:0099086 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko03460 ko05200 ko05210 ko05213 ko05226 map01524 map03430 map03460 map05200 map05210 map05213 map05226

Pfam Domains

Protein Families

DNA_mis_repair HATPase_c_3 Mlh1_C

Protein Analysis

671

Amino Acids

75.37

Weight (kDa)

5.77

Isoelectric Point (pI)

48.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_mis_repair	PF01119	166 - 286	8.4e-29	DNA mismatch repair protein, C-terminal domain
Mlh1_C	PF16413	383 - 670	3.6e-82	DNA mismatch repair protein Mlh1 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001742)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09140 AT4G09140
fragaria_vesca	FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560 FvH4_5g31560
malus_domestica	MD12G1033300.v1.1 MD14G1034200.v1.1
prunus_persica	Prupe.1G555900_v2.0.a1 Prupe.1G555900_v2.0.a1 Prupe.1G555900_v2.0.a1 Prupe.1G555900_v2.0.a1 Prupe.1G555900_v2.0.a1
pyrus_communis	pycom12g01730 pycom12g03050
rosa_chinensis	RchiOBHm_Chr3g0494621 RchiOBHm_Chr7g0226931 RchiOBHm_Chr7g0226941
rosa_laevigata	RLG00000001681
rosa_multiflora	Rmu_co8507019.1_g000001 Rmu_sc0000356.1_g000011 Rmu_sc0002762.1_g000004 Rmu_sc0008925.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018710 Rroxscaffold_3G00232410 Rroxscaffold_6G00391010
rosa_rugosa	Rorug03G0266200 Rorug07G0240400
rosa_samantha	Rh3AG316100 Rh3BG351600 Rh3CG348200 Rh3DG350800 Rh7AG388700 Rh7BG374500 Rh7BG374700 Rh7CG407900 Rh7DG386700
rosa_wichuraiana	Rw3G027600 Rw7G032590 Rw7G032610 Rw7G032620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	270
AatII	GACGTC	2 cut(s)	128, 1815
Acc36I	ACCTGC	1 cut(s)	105
AccBSI	CCGCTC	3 cut(s)	8, 115, 671
AccI	GTMKAC	1 cut(s)	1201
AccII	CGCG	1 cut(s)	1878
AciI	CCGC	5 cut(s)	6, 41, 115, 669, 1140
AclWI	GGATC	6 cut(s)	97, 554, 1052, 1418, 1431, 1602
AcoI	YGGCCR	2 cut(s)	73, 1066
AcsI	RAATTY	3 cut(s)	1546, 1687, 1936
AcuI	CTGAAG	6 cut(s)	534, 695, 1142, 1247, 1406, 1797
AcyI	GRCGYC	2 cut(s)	125, 1812
AfaI	GTAC	4 cut(s)	206, 341, 1786, 1965
AfiI	CCNNNNNNNGG	1 cut(s)	1387
AflIII	ACRYGT	2 cut(s)	738, 763
AgsI	TTSAA	8 cut(s)	372, 606, 851, 933, 945, 1076, 1664, 1927
AhdI	GACNNNNNGTC	1 cut(s)	154
AhlI	ACTAGT	1 cut(s)	1843
AjiI	CACGTC	1 cut(s)	217
Alw21I	GWGCWC	4 cut(s)	291, 685, 1317, 1839
Alw26I	GTCTC	3 cut(s)	67, 869, 1210
AlwI	GGATC	6 cut(s)	97, 554, 1052, 1418, 1431, 1602
AoxI	GGCC	4 cut(s)	42, 73, 241, 1066
ApeKI	GCWGC	5 cut(s)	308, 446, 701, 1499, 1676
ApoI	RAATTY	3 cut(s)	1546, 1687, 1936
Asp700I	GAANNNNTTC	2 cut(s)	940, 1012
AspLEI	GCGC	2 cut(s)	308, 1878
AspS9I	GGNCC	2 cut(s)	94, 241
AsuHPI	GGTGA	5 cut(s)	211, 217, 773, 951, 1742
AsuII	TTCGAA	1 cut(s)	2001
AsuNHI	GCTAGC	1 cut(s)	1085
AvaII	GGWCC	1 cut(s)	94
BalI	TGGCCA	1 cut(s)	1068
BamHI	GGATCC	1 cut(s)	1423
BanII	GRGCYC	3 cut(s)	685, 1317, 1873
BauI	CACGAG	1 cut(s)	1062
Bbv12I	GWGCWC	4 cut(s)	291, 685, 1317, 1839
BbvI	GCAGC	5 cut(s)	295, 433, 688, 1486, 1663
BceAI	ACGGC	3 cut(s)	54, 88, 492
BcgI	CGANNNNNNTGC	6 cut(s)	483, 517, 1445, 1479, 1829, 1863
BclI	TGATCA	1 cut(s)	1573
BcoDI	GTCTC	3 cut(s)	67, 869, 1210
BcuI	ACTAGT	1 cut(s)	1843
BfaI	CTAG	6 cut(s)	450, 567, 686, 1086, 1554, 1844
BfmI	CTRYAG	1 cut(s)	430
BfuAI	ACCTGC	1 cut(s)	105
BglII	AGATCT	1 cut(s)	1103
BisI	GCNGC	7 cut(s)	42, 116, 309, 447, 702, 1500, 1677
BlpI	GCTNAGC	2 cut(s)	389, 1872
BlsI	GCNGC	7 cut(s)	43, 117, 310, 448, 703, 1501, 1678
Bme18I	GGWCC	1 cut(s)	94
BmeRI	GACNNNNNGTC	1 cut(s)	154
BmgBI	CACGTC	1 cut(s)	217
BmgT120I	GGNCC	2 cut(s)	94, 241
BmiI	GGNNCC	2 cut(s)	987, 1425
BmsI	GCATC	6 cut(s)	481, 488, 728, 977, 1711, 1997
BmtI	GCTAGC	1 cut(s)	1089
BpmI	CTGGAG	2 cut(s)	1821, 2000
Bpu1102I	GCTNAGC	2 cut(s)	389, 1872
Bpu14I	TTCGAA	1 cut(s)	2001
BpuEI	CTTGAG	2 cut(s)	1324, 1472
BsaAI	YACGTR	1 cut(s)	208
BsaBI	GATNNNNATC	3 cut(s)	558, 945, 1275
BsaHI	GRCGYC	2 cut(s)	125, 1812
BsaJI	CCNNGG	3 cut(s)	237, 474, 630
BsaWI	WCCGGW	1 cut(s)	1992
Bsc4I	CCNNNNNNNGG	1 cut(s)	1387
Bse118I	RCCGGY	1 cut(s)	393
Bse1I	ACTGG	6 cut(s)	244, 989, 1209, 1562, 1804, 1983
Bse3DI	GCAATG	1 cut(s)	707
Bse8I	GATNNNNATC	3 cut(s)	558, 945, 1275
BseDI	CCNNGG	3 cut(s)	237, 474, 630
BseGI	GGATG	4 cut(s)	53, 592, 914, 1702
BseJI	GATNNNNATC	3 cut(s)	558, 945, 1275
BseLI	CCNNNNNNNGG	1 cut(s)	1387
BseMI	GCAATG	1 cut(s)	707
BseMII	CTCAG	1 cut(s)	1886
BseNI	ACTGG	6 cut(s)	244, 989, 1209, 1562, 1804, 1983
BseRI	GAGGAG	4 cut(s)	195, 1430, 1433, 1658
BseXI	GCAGC	5 cut(s)	295, 433, 688, 1486, 1663
BsgI	GTGCAG	2 cut(s)	1207, 1662
Bsh1236I	CGCG	1 cut(s)	1878
Bsh1285I	CGRYCG	1 cut(s)	503
BshFI	GGCC	4 cut(s)	44, 75, 243, 1068
BsiEI	CGRYCG	1 cut(s)	503
BsiHKAI	GWGCWC	4 cut(s)	291, 685, 1317, 1839
BsiSI	CCGG	2 cut(s)	394, 1993
BsiWI	CGTACG	1 cut(s)	204
BslFI	GGGAC	1 cut(s)	1586
BslI	CCNNNNNNNGG	1 cut(s)	1387
BsmAI	GTCTC	3 cut(s)	67, 869, 1210
BsmFI	GGGAC	1 cut(s)	1586
BsmI	GAATGC	2 cut(s)	1087, 1436
BsnI	GGCC	4 cut(s)	44, 75, 243, 1068
Bsp119I	TTCGAA	1 cut(s)	2001
Bsp1286I	GDGCHC	5 cut(s)	291, 685, 1317, 1839, 1873
Bsp143I	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
Bsp1720I	GCTNAGC	2 cut(s)	389, 1872
Bsp19I	CCATGG	1 cut(s)	630
BspACI	CCGC	5 cut(s)	6, 41, 115, 669, 1140
BspANI	GGCC	4 cut(s)	44, 75, 243, 1068
BspCNI	CTCAG	1 cut(s)	1885
BspFNI	CGCG	1 cut(s)	1878
BspHI	TCATGA	2 cut(s)	1444, 1815
BspLI	GGNNCC	2 cut(s)	987, 1425
BspMAI	CTGCAG	1 cut(s)	434
BspMI	ACCTGC	1 cut(s)	105
BspOI	GCTAGC	1 cut(s)	1089
BspPI	GGATC	6 cut(s)	97, 554, 1052, 1418, 1431, 1602
BspT104I	TTCGAA	1 cut(s)	2001
BsrBI	CCGCTC	3 cut(s)	8, 115, 671
BsrDI	GCAATG	1 cut(s)	707
BsrFI	RCCGGY	1 cut(s)	393
BsrI	ACTGG	6 cut(s)	244, 989, 1209, 1562, 1804, 1983
BssAI	RCCGGY	1 cut(s)	393
BssECI	CCNNGG	3 cut(s)	237, 474, 630
BssMI	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
BssNI	GRCGYC	2 cut(s)	125, 1812
BssSI	CACGAG	1 cut(s)	1062
BssT1I	CCWWGG	2 cut(s)	237, 630
Bst2BI	CACGAG	1 cut(s)	1062
Bst4CI	ACNGT	2 cut(s)	223, 840
Bst6I	CTCTTC	1 cut(s)	671
BstACI	GRCGYC	2 cut(s)	125, 1812
BstAPI	GCANNNNNTGC	2 cut(s)	1333, 1862
BstBAI	YACGTR	1 cut(s)	208
BstBI	TTCGAA	1 cut(s)	2001
BstC8I	GCNNGC	7 cut(s)	6, 196, 304, 526, 1087, 1377, 1739
BstDEI	CTNAG	3 cut(s)	389, 1118, 1872
BstDSI	CCRYGG	2 cut(s)	474, 630
BstF5I	GGATG	4 cut(s)	53, 592, 914, 1702
BstFNI	CGCG	1 cut(s)	1878
BstHHI	GCGC	2 cut(s)	308, 1878
BstKTI	GATC	9 cut(s)	105, 503, 562, 1060, 1106, 1147, 1426, 1576, 1597
BstMAI	GTCTC	3 cut(s)	67, 869, 1210
BstMBI	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
BstMCI	CGRYCG	1 cut(s)	503
BstMWI	GCNNNNNNNGC	5 cut(s)	308, 429, 452, 1333, 1862
BstNSI	RCATGY	4 cut(s)	742, 767, 1043, 1898
BstSFI	CTRYAG	1 cut(s)	430
BstSNI	TACGTA	1 cut(s)	208
BstUI	CGCG	1 cut(s)	1878
BstV1I	GCAGC	5 cut(s)	295, 433, 688, 1486, 1663
BstX2I	RGATCY	4 cut(s)	559, 1057, 1103, 1423
BstXI	CCANNNNNNTGG	2 cut(s)	251, 925
BstYI	RGATCY	4 cut(s)	559, 1057, 1103, 1423
BsuRI	GGCC	4 cut(s)	44, 75, 243, 1068
BtgI	CCRYGG	2 cut(s)	474, 630
BtrI	CACGTC	1 cut(s)	217
BtsCI	GGATG	4 cut(s)	53, 592, 914, 1702
BtsI	GCAGTG	3 cut(s)	1219, 1256, 1441
BtsIMutI	CAGTG	7 cut(s)	849, 996, 1219, 1256, 1382, 1441, 1976
BveI	ACCTGC	1 cut(s)	105
Cac8I	GCNNGC	7 cut(s)	6, 196, 304, 526, 1087, 1377, 1739
CciI	TCATGA	2 cut(s)	1444, 1815
CfoI	GCGC	2 cut(s)	308, 1878
Cfr10I	RCCGGY	1 cut(s)	393
Cfr13I	GGNCC	2 cut(s)	94, 241
CseI	GACGC	1 cut(s)	1325
Csp6I	GTAC	4 cut(s)	205, 340, 1785, 1964
CspCI	CAANNNNNGTGG	2 cut(s)	314, 349
CviQI	GTAC	4 cut(s)	205, 340, 1785, 1964
DdeI	CTNAG	3 cut(s)	389, 1118, 1872
DpnI	GATC	9 cut(s)	104, 502, 561, 1059, 1105, 1146, 1425, 1575, 1596
DpnII	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
DriI	GACNNNNNGTC	1 cut(s)	154
EaeI	YGGCCR	2 cut(s)	73, 1066
Eam1104I	CTCTTC	1 cut(s)	671
Eam1105I	GACNNNNNGTC	1 cut(s)	154
EarI	CTCTTC	1 cut(s)	671
Ecl136II	GAGCTC	2 cut(s)	683, 1315
Eco105I	TACGTA	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	237, 630
Eco24I	GRGCYC	3 cut(s)	685, 1317, 1873
Eco47I	GGWCC	1 cut(s)	94
Eco53kI	GAGCTC	2 cut(s)	683, 1315
Eco57I	CTGAAG	6 cut(s)	534, 695, 1142, 1247, 1406, 1797
EcoICRI	GAGCTC	2 cut(s)	683, 1315
EcoO109I	RGGNCCY	1 cut(s)	94
EcoT14I	CCWWGG	2 cut(s)	237, 630
EcoT22I	ATGCAT	2 cut(s)	1704, 1867
EcoT38I	GRGCYC	3 cut(s)	685, 1317, 1873
ErhI	CCWWGG	2 cut(s)	237, 630
FaqI	GGGAC	1 cut(s)	1586
FauI	CCCGC	1 cut(s)	13
FauNDI	CATATG	1 cut(s)	411
FbaI	TGATCA	1 cut(s)	1573
FblI	GTMKAC	1 cut(s)	1201
Fnu4HI	GCNGC	7 cut(s)	42, 116, 309, 447, 702, 1500, 1677
FokI	GGATG	4 cut(s)	40, 599, 901, 1689
FriOI	GRGCYC	3 cut(s)	685, 1317, 1873
Fsp4HI	GCNGC	7 cut(s)	42, 116, 309, 447, 702, 1500, 1677
FspBI	CTAG	6 cut(s)	450, 567, 686, 1086, 1554, 1844
GlaI	GCGC	2 cut(s)	307, 1877
GluI	GCNGC	7 cut(s)	42, 116, 309, 447, 702, 1500, 1677
GsuI	CTGGAG	2 cut(s)	1821, 2000
HaeIII	GGCC	4 cut(s)	44, 75, 243, 1068
HapII	CCGG	2 cut(s)	394, 1993
HgaI	GACGC	1 cut(s)	1325
HhaI	GCGC	2 cut(s)	308, 1878
Hin1I	GRCGYC	2 cut(s)	125, 1812
Hin6I	GCGC	2 cut(s)	306, 1876
HinP1I	GCGC	2 cut(s)	306, 1876
HindIII	AAGCTT	1 cut(s)	546
HinfI	GANTC	6 cut(s)	602, 811, 941, 1013, 1441, 1771
HpaII	CCGG	2 cut(s)	394, 1993
HphI	GGTGA	5 cut(s)	211, 217, 773, 951, 1742
Hpy166II	GTNNAC	3 cut(s)	781, 1202, 1300
Hpy8I	GTNNAC	3 cut(s)	781, 1202, 1300
Hpy99I	CGWCG	8 cut(s)	28, 31, 71, 74, 127, 130, 1341, 1814
HpyAV	CCTTC	8 cut(s)	292, 573, 739, 815, 918, 1166, 1381, 1670
HpyCH4III	ACNGT	2 cut(s)	223, 840
HpyCH4IV	ACGT	7 cut(s)	23, 125, 203, 207, 216, 332, 1812
HpyF10VI	GCNNNNNNNGC	5 cut(s)	308, 429, 452, 1333, 1862
HpyF3I	CTNAG	3 cut(s)	389, 1118, 1872
HpySE526I	ACGT	7 cut(s)	23, 125, 203, 207, 216, 332, 1812
Hsp92I	GRCGYC	2 cut(s)	125, 1812
HspAI	GCGC	2 cut(s)	306, 1876
Ksp22I	TGATCA	1 cut(s)	1573
Kzo9I	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
LmnI	GCTCC	6 cut(s)	13, 286, 443, 575, 872, 1162
Lsp1109I	GCAGC	5 cut(s)	295, 433, 688, 1486, 1663
LweI	GCATC	6 cut(s)	481, 488, 728, 977, 1711, 1997
MaeI	CTAG	6 cut(s)	450, 567, 686, 1086, 1554, 1844
MaeII	ACGT	7 cut(s)	23, 125, 203, 207, 216, 332, 1812
MaeIII	GTNAC	3 cut(s)	217, 223, 750
MalI	GATC	9 cut(s)	104, 502, 561, 1059, 1105, 1146, 1425, 1575, 1596
MbiI	CCGCTC	3 cut(s)	8, 115, 671
MboI	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
MboII	GAAGA	7 cut(s)	634, 688, 844, 903, 1098, 1471, 1790
MfeI	CAATTG	1 cut(s)	1186
MflI	RGATCY	4 cut(s)	559, 1057, 1103, 1423
MhlI	GDGCHC	5 cut(s)	291, 685, 1317, 1839, 1873
MlsI	TGGCCA	1 cut(s)	1068
MluNI	TGGCCA	1 cut(s)	1068
MmeI	TCCRAC	1 cut(s)	90
Mox20I	TGGCCA	1 cut(s)	1068
Mph1103I	ATGCAT	2 cut(s)	1704, 1867
MroXI	GAANNNNTTC	2 cut(s)	940, 1012
MscI	TGGCCA	1 cut(s)	1068
MseI	TTAA	3 cut(s)	462, 1353, 1452
MslI	CAYNNNNRTG	5 cut(s)	414, 737, 768, 1235, 1353
Msp20I	TGGCCA	1 cut(s)	1068
MspA1I	CMGCKG	3 cut(s)	1097, 1142, 1676
MspI	CCGG	2 cut(s)	394, 1993
MunI	CAATTG	1 cut(s)	1186
Mva1269I	GAATGC	2 cut(s)	1087, 1436
MvnI	CGCG	1 cut(s)	1878
MwoI	GCNNNNNNNGC	5 cut(s)	308, 429, 452, 1333, 1862
NcoI	CCATGG	1 cut(s)	630
NdeI	CATATG	1 cut(s)	411
NdeII	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
NheI	GCTAGC	1 cut(s)	1085
NlaIV	GGNNCC	2 cut(s)	987, 1425
NmeAIII	GCCGAG	1 cut(s)	116
NmuCI	GTSAC	2 cut(s)	217, 223
NsiI	ATGCAT	2 cut(s)	1704, 1867
NspI	RCATGY	4 cut(s)	742, 767, 1043, 1898
NspV	TTCGAA	1 cut(s)	2001
PagI	TCATGA	2 cut(s)	1444, 1815
PciI	ACATGT	2 cut(s)	738, 763
PcsI	WCGNNNNNNNCGW	2 cut(s)	164, 213
PctI	GAATGC	2 cut(s)	1087, 1436
PdmI	GAANNNNTTC	2 cut(s)	940, 1012
PfeI	GAWTC	6 cut(s)	602, 811, 941, 1013, 1441, 1771
Pfl23II	CGTACG	1 cut(s)	204
PflFI	GACNNNGTC	1 cut(s)	1811
PkrI	GCNGC	7 cut(s)	43, 117, 310, 448, 703, 1501, 1678
Ple19I	CGATCG	1 cut(s)	503
Ppu21I	YACGTR	1 cut(s)	208
PpuMI	RGGWCCY	1 cut(s)	94
PscI	ACATGT	2 cut(s)	738, 763
PsiI	TTATAA	1 cut(s)	270
Psp124BI	GAGCTC	2 cut(s)	685, 1317
Psp5II	RGGWCCY	1 cut(s)	94
PspLI	CGTACG	1 cut(s)	204
PspN4I	GGNNCC	2 cut(s)	987, 1425
PspPI	GGNCC	2 cut(s)	94, 241
PspPPI	RGGWCCY	1 cut(s)	94
PstI	CTGCAG	1 cut(s)	434
PsuI	RGATCY	4 cut(s)	559, 1057, 1103, 1423
PsyI	GACNNNGTC	1 cut(s)	1811
PvuI	CGATCG	1 cut(s)	503
PvuII	CAGCTG	2 cut(s)	1097, 1676
RsaI	GTAC	4 cut(s)	206, 341, 1786, 1965
RsaNI	GTAC	4 cut(s)	205, 340, 1785, 1964
RseI	CAYNNNNRTG	5 cut(s)	414, 737, 768, 1235, 1353
SacI	GAGCTC	2 cut(s)	685, 1317
SaqAI	TTAA	3 cut(s)	462, 1353, 1452
SatI	GCNGC	7 cut(s)	42, 116, 309, 447, 702, 1500, 1677
Sau3AI	GATC	9 cut(s)	102, 500, 559, 1057, 1103, 1144, 1423, 1573, 1594
Sau96I	GGNCC	2 cut(s)	94, 241
SduI	GDGCHC	5 cut(s)	291, 685, 1317, 1839, 1873
SfaNI	GCATC	6 cut(s)	481, 488, 728, 977, 1711, 1997
SfcI	CTRYAG	1 cut(s)	430
SfuI	TTCGAA	1 cut(s)	2001
SinI	GGWCC	1 cut(s)	94
SmiMI	CAYNNNNRTG	5 cut(s)	414, 737, 768, 1235, 1353
SmlI	CTYRAG	2 cut(s)	1303, 1487
SmoI	CTYRAG	2 cut(s)	1303, 1487
SnaBI	TACGTA	1 cut(s)	208
SpeI	ACTAGT	1 cut(s)	1843
SsiI	CCGC	5 cut(s)	6, 41, 115, 669, 1140
SspMI	CTAG	6 cut(s)	450, 567, 686, 1086, 1554, 1844
SstI	GAGCTC	2 cut(s)	685, 1317
StyI	CCWWGG	2 cut(s)	237, 630
TaaI	ACNGT	2 cut(s)	223, 840
TaiI	ACGT	7 cut(s)	26, 128, 206, 210, 219, 335, 1815
TaqI	TCGA	6 cut(s)	128, 158, 167, 499, 903, 2001
TauI	GCSGC	2 cut(s)	44, 118
TfiI	GAWTC	6 cut(s)	602, 811, 941, 1013, 1441, 1771
Tru1I	TTAA	3 cut(s)	462, 1353, 1452
Tru9I	TTAA	3 cut(s)	462, 1353, 1452
TscAI	CASTG	7 cut(s)	849, 996, 1226, 1263, 1389, 1441, 1983
TseFI	GTSAC	2 cut(s)	217, 223
TseI	GCWGC	5 cut(s)	308, 446, 701, 1499, 1676
Tsp45I	GTSAC	2 cut(s)	217, 223
TspGWI	ACGGA	3 cut(s)	562, 1025, 1968
TspRI	CASTG	7 cut(s)	849, 996, 1226, 1263, 1389, 1441, 1983
Tth111I	GACNNNGTC	1 cut(s)	1811
VpaK11BI	GGWCC	1 cut(s)	94
XapI	RAATTY	3 cut(s)	1546, 1687, 1936
XbaI	TCTAGA	1 cut(s)	685
XceI	RCATGY	4 cut(s)	742, 767, 1043, 1898
XmiI	GTMKAC	1 cut(s)	1201
XmnI	GAANNNNTTC	2 cut(s)	940, 1012
XspI	CTAG	6 cut(s)	450, 567, 686, 1086, 1554, 1844
ZraI	GACGTC	2 cut(s)	126, 1813
Zsp2I	ATGCAT	2 cut(s)	1704, 1867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.