MD12G1114000.v1.1

UDP-glycosyltransferase 86A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002191
Physical Location & Seq
Reverse (-)
18152385 .. 18154533
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1114000.v1.1.491

Sequence Viewer

Length: 1440 bp
ATGGAGGAAAATCAGTCAAAGCCTCACGCAATCATGGTGCCTCTTCCTATCCAAGGCCATGTAATCCCATTCACCAATCTAGCCATGAAGCTTGCATCAAATGGTTTCACCATCACTTTTGTCAACACCAAATGTGTTCACCGCCAGCTACTCAAATCACAACCCAACAACAACTCTGGAGATCAAGATGACATCTTCTCCAAAGTGCGGGAATCCGGCCTAGACATACGTTACACAACGGTGAGTGATGGTCTTCCATTAGCCTTCAACCGATTTCAGAATCTCGACCAATTTCTCGAGTGTTCTTTGCATGTCTTCCCTGCTCATGTTGATGAACTTGTTGGAGATTTAGTCCAATCTGACCCCTCAATCTCTTGCTTGATCGCTGATACCTACCACTGTTGGCCAGCAACCATAGCCAACAAGTACAATCTTGTCCATATTTCTTTCTGGACTCAACCAGCTCTAGTTTTTAACATCTACTATCACTTGGACCTCCTCATAAAAAATGGTCATTTTGGTTCTCAAGATAATCGTGAGGGCACCATCGATTACATACCTGGAGTGAAGGCTATTGAACCAAAGGACTTGATGTCAAACTTGCAAGAAACAGATATATCAACACCGATGCACCGCGTCATATACAAGGCATACGAGGAAGTGAAAGGGGCAGATTTCATATTGTGTAATACAGTGCAAGAACTTGAATCCGAGTCCCTCTCAGCCTTGCAAGAGAAGCAACCCACCTATGCAATTGGTCCTGTTCTCTCCAATAAACCTACTAAGGGTATGGTGGCTACCAATTTAATGTCAGAGTTTGACTGTACTCAGTGGCTCCACACTAAGCCTCATGGTTCAGTTTTGTTCATTTCATTTGGAAGCTATGGTCAAGTTACTAAAAATGATTTCGAAGAAATAGCTCATGGACTTTTGCTTAGTAAAGTGAGTTTCATCTGGGTGCTTCGCCCGGACACAACAAGTTATGATGAAACATGTATCATGCCGGTCGGATTTGAGGACGAGACTAAAGATAGAGGGTTGATGGTGCCATGGTGCTCTCAGATTGAGGTGCTTTTGCATCCAGCAATAGGAGGGTTCTTAACACATTGTGGATGGAATTCCATACTTGAAAGTATGCGGTGTGGTGTTCCCATGTTGTGTTTTCCTCTATGGACTGACCAAATCACTAATAGGAAACTAGTAGTTGATGATTGGGGGATTGGACTAAATCTTTGTGATACAGTTAAACTCGTTACAAGGGGAGAAGTAGGGGAGAAGATCAACCGTCTGATGTGTGGAGATTCGGGTGATGGATTACAGAAACAGATCAAGAAAGTAAGGCAAACTTTAGAGGACGCATTGGCTGTAAATGGGTCATCAGAAAGAAATTTATCTCAATTCATTTGTGGCGTTAAGGAAAAAATTCGGACACGAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0080043 GO:0080044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UDPGT

Protein Analysis

480

Amino Acids

53.72

Weight (kDa)

5.53

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 209 - 407 1.7e-19 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 37, 542, 1045
AccII CGCG 1 cut(s) 636
AciI CCGC 4 cut(s) 142, 208, 634, 1138
AcoI YGGCCR 1 cut(s) 404
AcsI RAATTY 3 cut(s) 1117, 1387, 1422
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 2 cut(s) 428, 826
AfiI CCNNNNNNNGG 4 cut(s) 53, 207, 785, 1088
AflIII ACRYGT 1 cut(s) 992
AgsI TTSAA 4 cut(s) 268, 578, 707, 1130
AhdI GACNNNNNGTC 1 cut(s) 592
AhlI ACTAGT 1 cut(s) 1198
AjnI CCWGG 1 cut(s) 559
AleI CACNNNNGTG 2 cut(s) 239, 1435
AluBI AGCT 5 cut(s) 91, 148, 464, 882, 920
AluI AGCT 5 cut(s) 91, 148, 464, 882, 920
Alw21I GWGCWC 1 cut(s) 1058
Alw26I GTCTC 1 cut(s) 1016
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 3 cut(s) 55, 217, 404
ApoI RAATTY 3 cut(s) 1117, 1387, 1422
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
Asp700I GAANNNNTTC 1 cut(s) 1422
AspS9I GGNCC 2 cut(s) 493, 758
AsuC2I CCSGG 1 cut(s) 968
AsuHPI GGTGA 5 cut(s) 64, 100, 131, 253, 1319
AsuII TTCGAA 1 cut(s) 909
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 2 cut(s) 493, 758
BaeGI GKGCMC 1 cut(s) 545
BalI TGGCCA 1 cut(s) 406
BanI GGYRCC 3 cut(s) 37, 542, 1045
BauI CACGAG 1 cut(s) 1431
BbsI GAAGAC 2 cut(s) 245, 307
Bbv12I GWGCWC 1 cut(s) 1058
BccI CCATC 6 cut(s) 119, 242, 554, 1036, 1107, 1304
BciT130I CCWGG 1 cut(s) 561
BcnI CCSGG 1 cut(s) 968
BcoDI GTCTC 1 cut(s) 1016
BcuI ACTAGT 1 cut(s) 1198
BfaI CTAG 4 cut(s) 80, 221, 467, 1199
Bme1390I CCNGG 2 cut(s) 561, 968
Bme18I GGWCC 2 cut(s) 493, 758
BmeRI GACNNNNNGTC 1 cut(s) 592
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 493, 758
BmiI GGNNCC 4 cut(s) 39, 544, 836, 1047
BmrFI CCNGG 2 cut(s) 561, 968
BmsI GCATC 3 cut(s) 104, 618, 1087
BpiI GAAGAC 2 cut(s) 245, 307
BplI GAGNNNNNCTC 2 cut(s) 704, 736
BpmI CTGGAG 2 cut(s) 198, 582
Bpu14I TTCGAA 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 968
Bsa29I ATCGAT 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 52, 1049
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 336, 366
Bsc4I CCNNNNNNNGG 4 cut(s) 53, 207, 785, 1088
Bse118I RCCGGY 1 cut(s) 1003
BseBI CCWGG 1 cut(s) 561
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 52, 1049
BseGI GGATG 2 cut(s) 1078, 1118
BseLI CCNNNNNNNGG 4 cut(s) 53, 207, 785, 1088
BseMII CTCAG 3 cut(s) 735, 842, 1073
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 545
Bsh1236I CGCG 1 cut(s) 636
Bsh1285I CGRYCG 1 cut(s) 1008
BshFI GGCC 3 cut(s) 57, 219, 406
BshNI GGYRCC 3 cut(s) 37, 542, 1045
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 1008
BsiHKAI GWGCWC 1 cut(s) 1058
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 3 cut(s) 216, 968, 1004
BslFI GGGAC 1 cut(s) 700
BslI CCNNNNNNNGG 4 cut(s) 53, 207, 785, 1088
BsmAI GTCTC 1 cut(s) 1016
BsmFI GGGAC 1 cut(s) 700
BsnI GGCC 3 cut(s) 57, 219, 406
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 909
Bsp1286I GDGCHC 2 cut(s) 545, 1058
Bsp143I GATC 4 cut(s) 181, 381, 1278, 1326
Bsp19I CCATGG 1 cut(s) 1049
BspACI CCGC 4 cut(s) 142, 208, 634, 1138
BspANI GGCC 3 cut(s) 57, 219, 406
BspCNI CTCAG 3 cut(s) 734, 841, 1072
BspDI ATCGAT 1 cut(s) 549
BspFNI CGCG 1 cut(s) 636
BspLI GGNNCC 4 cut(s) 39, 544, 836, 1047
BspT104I TTCGAA 1 cut(s) 909
BspT107I GGYRCC 3 cut(s) 37, 542, 1045
BsrFI RCCGGY 1 cut(s) 1003
BssAI RCCGGY 1 cut(s) 1003
BssECI CCNNGG 2 cut(s) 52, 1049
BssMI GATC 4 cut(s) 181, 381, 1278, 1326
BssSI CACGAG 1 cut(s) 1431
BssT1I CCWWGG 2 cut(s) 52, 1049
Bst2BI CACGAG 1 cut(s) 1431
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 6 cut(s) 241, 401, 694, 824, 1243, 1286
Bst6I CTCTTC 1 cut(s) 48
BstBI TTCGAA 1 cut(s) 909
BstC8I GCNNGC 3 cut(s) 93, 146, 408
BstDEI CTNAG 6 cut(s) 721, 783, 828, 843, 935, 1059
BstDSI CCRYGG 1 cut(s) 1049
BstENI CCTNNNNNAGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 1078, 1118
BstFNI CGCG 1 cut(s) 636
BstKTI GATC 4 cut(s) 184, 384, 1281, 1329
BstMAI GTCTC 1 cut(s) 1016
BstMBI GATC 4 cut(s) 181, 381, 1278, 1326
BstMCI CGRYCG 1 cut(s) 1008
BstMWI GCNNNNNNNGC 2 cut(s) 416, 736
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 2 cut(s) 314, 996
BstSCI CCNGG 2 cut(s) 559, 966
BstSLI GKGCMC 1 cut(s) 545
BstUI CGCG 1 cut(s) 636
BstV2I GAAGAC 2 cut(s) 245, 307
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 3 cut(s) 57, 219, 406
BsuTUI ATCGAT 1 cut(s) 549
BtgI CCRYGG 1 cut(s) 1049
BtsCI GGATG 2 cut(s) 1078, 1118
BtsIMutI CAGTG 3 cut(s) 397, 699, 836
Cac8I GCNNGC 3 cut(s) 93, 146, 408
Cfr10I RCCGGY 1 cut(s) 1003
Cfr13I GGNCC 2 cut(s) 493, 758
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 2 cut(s) 625, 1364
Csp6I GTAC 2 cut(s) 427, 825
CviQI GTAC 2 cut(s) 427, 825
DdeI CTNAG 6 cut(s) 721, 783, 828, 843, 935, 1059
DpnI GATC 4 cut(s) 183, 383, 1280, 1328
DpnII GATC 4 cut(s) 181, 381, 1278, 1326
DraIII CACNNNGTG 1 cut(s) 1109
DriI GACNNNNNGTC 1 cut(s) 592
EaeI YGGCCR 1 cut(s) 404
Eam1104I CTCTTC 1 cut(s) 48
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 52, 1049
Eco47I GGWCC 2 cut(s) 493, 758
Eco88I CYCGRG 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 1117
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 2 cut(s) 52, 1049
ErhI CCWWGG 2 cut(s) 52, 1049
FalI AAGNNNNNCTT 2 cut(s) 1330, 1362
FaqI GGGAC 1 cut(s) 700
FauI CCCGC 1 cut(s) 201
FokI GGATG 2 cut(s) 1065, 1125
FspBI CTAG 4 cut(s) 80, 221, 467, 1199
GsuI CTGGAG 2 cut(s) 198, 582
HaeIII GGCC 3 cut(s) 57, 219, 406
HapII CCGG 3 cut(s) 216, 968, 1004
HgaI GACGC 2 cut(s) 625, 1364
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 6 cut(s) 212, 280, 454, 707, 713, 1301
HpaII CCGG 3 cut(s) 216, 968, 1004
HphI GGTGA 5 cut(s) 64, 100, 131, 253, 1319
Hpy166II GTNNAC 2 cut(s) 124, 139
Hpy188I TCNGA 9 cut(s) 279, 361, 712, 814, 1010, 1062, 1290, 1381, 1428
Hpy188III TCNNGA 8 cut(s) 177, 185, 284, 296, 451, 527, 536, 1330
Hpy8I GTNNAC 2 cut(s) 124, 139
HpyAV CCTTC 2 cut(s) 274, 562
HpyCH4III ACNGT 6 cut(s) 241, 401, 694, 824, 1243, 1286
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 8 cut(s) 95, 310, 604, 631, 697, 730, 752, 1078
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 736
HpyF3I CTNAG 6 cut(s) 721, 783, 828, 843, 935, 1059
HpySE526I ACGT 1 cut(s) 229
Kzo9I GATC 4 cut(s) 181, 381, 1278, 1326
LmnI GCTCC 1 cut(s) 840
LweI GCATC 3 cut(s) 104, 618, 1087
MaeI CTAG 4 cut(s) 80, 221, 467, 1199
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 3 cut(s) 230, 892, 1252
MalI GATC 4 cut(s) 183, 383, 1280, 1328
MboI GATC 4 cut(s) 181, 381, 1278, 1326
MboII GAAGA 6 cut(s) 35, 187, 245, 307, 923, 1288
MfeI CAATTG 1 cut(s) 753
MhlI GDGCHC 2 cut(s) 545, 1058
MlsI TGGCCA 1 cut(s) 406
MluCI AATT 7 cut(s) 290, 753, 802, 1117, 1387, 1397, 1422
MluNI TGGCCA 1 cut(s) 406
MlyI GAGTC 2 cut(s) 448, 722
MmeI TCCRAC 2 cut(s) 322, 988
Mox20I TGGCCA 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 1422
MscI TGGCCA 1 cut(s) 406
MseI TTAA 5 cut(s) 474, 806, 1100, 1245, 1413
MslI CAYNNNNRTG 4 cut(s) 239, 330, 956, 1435
Msp20I TGGCCA 1 cut(s) 406
MspI CCGG 3 cut(s) 216, 968, 1004
MspR9I CCNGG 2 cut(s) 561, 968
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 1 cut(s) 561
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 2 cut(s) 416, 736
NciI CCSGG 1 cut(s) 968
NcoI CCATGG 1 cut(s) 1049
NdeII GATC 4 cut(s) 181, 381, 1278, 1326
NlaIV GGNNCC 4 cut(s) 39, 544, 836, 1047
NspI RCATGY 2 cut(s) 314, 996
NspV TTCGAA 1 cut(s) 909
OliI CACNNNNGTG 2 cut(s) 239, 1435
PaeR7I CTCGAG 1 cut(s) 296
PciI ACATGT 1 cut(s) 992
PdmI GAANNNNTTC 1 cut(s) 1422
PfeI GAWTC 4 cut(s) 212, 280, 707, 1301
PleI GAGTC 2 cut(s) 448, 721
PpsI GAGTC 2 cut(s) 448, 721
PscI ACATGT 1 cut(s) 992
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 4 cut(s) 39, 544, 836, 1047
PspPI GGNCC 2 cut(s) 493, 758
RsaI GTAC 2 cut(s) 428, 826
RsaNI GTAC 2 cut(s) 427, 825
RseI CAYNNNNRTG 4 cut(s) 239, 330, 956, 1435
SaqAI TTAA 5 cut(s) 474, 806, 1100, 1245, 1413
Sau3AI GATC 4 cut(s) 181, 381, 1278, 1326
Sau96I GGNCC 2 cut(s) 493, 758
SchI GAGTC 2 cut(s) 448, 722
ScrFI CCNGG 2 cut(s) 561, 968
SduI GDGCHC 2 cut(s) 545, 1058
SfaNI GCATC 3 cut(s) 104, 618, 1087
Sfr274I CTCGAG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 909
SinI GGWCC 2 cut(s) 493, 758
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 4 cut(s) 239, 330, 956, 1435
SmlI CTYRAG 2 cut(s) 296, 525
SmoI CTYRAG 2 cut(s) 296, 525
SpeI ACTAGT 1 cut(s) 1198
Sse9I AATT 7 cut(s) 290, 753, 802, 1117, 1387, 1397, 1422
SsiI CCGC 4 cut(s) 142, 208, 634, 1138
SspMI CTAG 4 cut(s) 80, 221, 467, 1199
StyD4I CCNGG 2 cut(s) 559, 966
StyI CCWWGG 2 cut(s) 52, 1049
TaaI ACNGT 6 cut(s) 241, 401, 694, 824, 1243, 1286
TaiI ACGT 1 cut(s) 232
TaqI TCGA 4 cut(s) 285, 297, 549, 909
TasI AATT 7 cut(s) 290, 753, 802, 1117, 1387, 1397, 1422
TatI WGTACW 2 cut(s) 426, 824
TfiI GAWTC 4 cut(s) 212, 280, 707, 1301
Tru1I TTAA 5 cut(s) 474, 806, 1100, 1245, 1413
Tru9I TTAA 5 cut(s) 474, 806, 1100, 1245, 1413
TscAI CASTG 3 cut(s) 404, 699, 836
TspDTI ATGAA 8 cut(s) 101, 348, 667, 856, 861, 940, 1002, 1390
TspRI CASTG 3 cut(s) 404, 699, 836
VpaK11BI GGWCC 2 cut(s) 493, 758
XagI CCTNNNNNAGG 1 cut(s) 51
XapI RAATTY 3 cut(s) 1117, 1387, 1422
XceI RCATGY 2 cut(s) 314, 996
XhoI CTCGAG 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 1422
XspI CTAG 4 cut(s) 80, 221, 467, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.