MD12G1114200.v1.1

UDP-glycosyltransferase 86A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002191
Physical Location & Seq
Reverse (-)
18186054 .. 18188256
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1114200.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGCAGTCATCTGTCCACACATCACAGAGATCGCACAATTAAGATGTGTTTTTCAGTTCATCTCACTTGCTTACATCCTCAGAAACACCAATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATATGGAGGACAAAGCCTCAAACCCTCATGCAATCATGGTGCCTCTTCCTCTCCAAGGCCATGTAACCCCATTGACACATCTGGCCACGAAGCTTGCTTCACAGGGCTTCACCATCACTTTTGTCAACACCCAATATATCCACCACCACATACTCAAGTCACAACCCAACGACAACACAGAAGACGATATCTTTGCCACGGCGCGTAAATCTGGTCTAGACATACGGTACACAACAGTGAGTGATGGATTTCCACTAGCTTTCAACCGAATCCAGAACCTCGACCAATCTCTTGAGGGTGCTTTGCATGTCTTCCCTGCCCATGTGGATGAACTTGTTGGAAATTTAGTCCAATCTGACCCCTCAATCACTTGTTTGATTGTTGATACTTTCCAATCATGGTCAGCAACCATAGCCAATAAGTACAACCTTATCCATATTTCCTTCTGGACTGAACCAGCTCTAGTGTTCAACATCTACTATCACTTGGACCTTCTGCAAAAGAATGGTCATTTCGGTTCTCATGATAATCGCGAGGACATCATTGATTACATACCTGGCGTGAAAGCTATTGAACCAAAGGACTTGATGTCACACATCCAATCAACAGATTTATTTTGCCCAATGCTCCGAACGTTTTACAAGTCATTTCGTGAAGTGAAAAAGGCAGATTTTATTTTGTGTAATACAGTGCAAGAACTTGAATTTGAGTCCCTTTCTGCCTTGCAAGCTAAGCAACCAACATATCCAATTGGCCCTGTCTTCCCCGATAATCTCACCAAGAATATCGTGGCTACCAACCTGATGCCAGAATTTGACTGCATCCATTGGCTCCACGAGAAGCCTCATGGTTCGGTTTTGTTCATTTCATTTGGAAGCTATGCTCAAGTTACAAAAGATGATTTTGAGGAAATAGCTCATGGGCTTTTGCTTAGTAAAGTGAATTTCATCTGGGTACTTCGTTCCGACACTACAGGTTATGATGAAACGTATATTCTACCAGTCGGATTTGAAGATGAGATTAAAGATAGAGGGTTGATGGTGCCATGGTGCTCTCAGATTGAGGTGCTTTCACATCCAGCAGTAGGAGGGTTCTTAACGCATTGTGGATGGAATTCTATACTGGAAAGTATGCGGTGTGGTGTTCCCATGCTATGTTTTCCTCTAGTGGCTGACCAAATCACTAATAGGAAACTAGTAGTTGATGATTGGGGCATTGGACTCAATCTTTGTGATCGGGTTAAACCAGTTAGAAGGGTGGAGGTAGCAGAGAAGATCAACCGTCTGATATGTGGAAAATCGGGTGACGGTTTGCAGAAGAAGGTCATGAAAGCAACACAAACATTGGAGGATGCAGTGGCTCTAAATGGGTCATCACAAAAGAACTTATCTCAATTCATTAGTAATGTGAAGGAAAAAATTCAGACACGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0080043 GO:0080044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UDPGT

Protein Analysis

525

Amino Acids

59.57

Weight (kDa)

5.93

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 329 - 452 7.8e-20 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 178, 1180
AccII CGCG 2 cut(s) 343, 672
AciI CCGC 1 cut(s) 1273
AclI AACGTT 1 cut(s) 773
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 6 cut(s) 481, 842, 950, 1081, 1252, 1557
AfaI GTAC 3 cut(s) 368, 563, 1095
AfiI CCNNNNNNNGG 2 cut(s) 194, 1223
AgsI TTSAA 5 cut(s) 403, 610, 713, 842, 1151
AhdI GACNNNNNGTC 1 cut(s) 727
AhlI ACTAGT 1 cut(s) 1333
AjnI CCWGG 1 cut(s) 694
AleI CACNNNNGTG 2 cut(s) 374, 1570
AluBI AGCT 7 cut(s) 232, 398, 599, 707, 869, 1017, 1055
AluI AGCT 7 cut(s) 232, 398, 599, 707, 869, 1017, 1055
Alw21I GWGCWC 1 cut(s) 1193
AoxI GGCC 3 cut(s) 196, 222, 892
ApoI RAATTY 6 cut(s) 481, 842, 950, 1081, 1252, 1557
ArsI GACNNNNNNTTYG 6 cut(s) 314, 346, 631, 663, 1350, 1382
Asp700I GAANNNNTTC 1 cut(s) 1557
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 628, 893
AsuHPI GGTGA 3 cut(s) 241, 907, 1454
AvaII GGWCC 1 cut(s) 628
BalI TGGCCA 1 cut(s) 224
BanI GGYRCC 2 cut(s) 178, 1180
BauI CACGAG 1 cut(s) 974
BbsI GAAGAC 3 cut(s) 327, 442, 892
Bbv12I GWGCWC 1 cut(s) 1193
BccI CCATC 4 cut(s) 260, 377, 1171, 1242
BceAI ACGGC 1 cut(s) 354
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BciT130I CCWGG 1 cut(s) 696
BcuI ACTAGT 1 cut(s) 1333
BfaI CTAG 5 cut(s) 356, 395, 602, 1304, 1334
BfmI CTRYAG 1 cut(s) 1110
BlpI GCTNAGC 1 cut(s) 870
Bme1390I CCNGG 1 cut(s) 696
Bme18I GGWCC 1 cut(s) 628
BmeRI GACNNNNNGTC 1 cut(s) 727
BmgT120I GGNCC 2 cut(s) 628, 893
BmiI GGNNCC 3 cut(s) 180, 971, 1182
BmrFI CCNGG 1 cut(s) 696
BmsI GCATC 3 cut(s) 933, 969, 1480
BpiI GAAGAC 3 cut(s) 327, 442, 892
Bpu1102I GCTNAGC 1 cut(s) 870
BpuEI CTTGAG 3 cut(s) 278, 452, 1008
BsaJI CCNNGG 3 cut(s) 193, 336, 1184
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 1223
Bse1I ACTGG 3 cut(s) 1139, 1266, 1385
BseBI CCWGG 1 cut(s) 696
BseDI CCNNGG 3 cut(s) 193, 336, 1184
BseGI GGATG 7 cut(s) 75, 472, 735, 960, 1213, 1253, 1495
BseLI CCNNNNNNNGG 2 cut(s) 194, 1223
BseMII CTCAG 2 cut(s) 94, 1208
BseNI ACTGG 3 cut(s) 1139, 1266, 1385
Bsh1236I CGCG 2 cut(s) 343, 672
BshFI GGCC 3 cut(s) 198, 224, 894
BshNI GGYRCC 2 cut(s) 178, 1180
BsiHKAI GWGCWC 1 cut(s) 1193
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 2 cut(s) 194, 1223
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 3 cut(s) 198, 224, 894
Bsp1286I GDGCHC 1 cut(s) 1193
Bsp143I GATC 3 cut(s) 30, 1372, 1413
Bsp1720I GCTNAGC 1 cut(s) 870
Bsp19I CCATGG 1 cut(s) 1184
Bsp68I TCGCGA 1 cut(s) 672
BspACI CCGC 1 cut(s) 1273
BspANI GGCC 3 cut(s) 198, 224, 894
BspCNI CTCAG 2 cut(s) 93, 1207
BspFNI CGCG 2 cut(s) 343, 672
BspHI TCATGA 2 cut(s) 661, 1464
BspLI GGNNCC 3 cut(s) 180, 971, 1182
BspT107I GGYRCC 2 cut(s) 178, 1180
BsrI ACTGG 3 cut(s) 1139, 1266, 1385
BssECI CCNNGG 3 cut(s) 193, 336, 1184
BssMI GATC 3 cut(s) 30, 1372, 1413
BssSI CACGAG 1 cut(s) 974
BssT1I CCWWGG 2 cut(s) 193, 1184
Bst2BI CACGAG 1 cut(s) 974
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 5 cut(s) 366, 376, 829, 1421, 1448
Bst6I CTCTTC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 234, 867
BstDEI CTNAG 4 cut(s) 80, 870, 1070, 1194
BstDSI CCRYGG 2 cut(s) 336, 1184
BstENI CCTNNNNNAGG 1 cut(s) 192
BstF5I GGATG 7 cut(s) 75, 472, 735, 960, 1213, 1253, 1495
BstFNI CGCG 2 cut(s) 343, 672
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 33, 1375, 1416
BstMBI GATC 3 cut(s) 30, 1372, 1413
BstMWI GCNNNNNNNGC 3 cut(s) 551, 866, 871
BstNI CCWGG 1 cut(s) 696
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 1 cut(s) 1110
BstUI CGCG 2 cut(s) 343, 672
BstV2I GAAGAC 3 cut(s) 327, 442, 892
BsuRI GGCC 3 cut(s) 198, 224, 894
BtgI CCRYGG 2 cut(s) 336, 1184
BtsCI GGATG 7 cut(s) 75, 472, 735, 960, 1213, 1253, 1495
BtsI GCAGTG 1 cut(s) 1500
BtsIMutI CAGTG 3 cut(s) 381, 834, 1500
BtuMI TCGCGA 1 cut(s) 672
Cac8I GCNNGC 2 cut(s) 234, 867
CciI TCATGA 2 cut(s) 661, 1464
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 628, 893
Csp6I GTAC 3 cut(s) 367, 562, 1094
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviQI GTAC 3 cut(s) 367, 562, 1094
DdeI CTNAG 4 cut(s) 80, 870, 1070, 1194
DpnI GATC 3 cut(s) 32, 1374, 1415
DpnII GATC 3 cut(s) 30, 1372, 1413
DriI GACNNNNNGTC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 189
Eam1105I GACNNNNNGTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 189
Eco130I CCWWGG 2 cut(s) 193, 1184
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 628
EcoNI CCTNNNNNAGG 1 cut(s) 192
EcoRI GAATTC 1 cut(s) 1252
EcoRII CCWGG 1 cut(s) 694
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 193, 1184
ErhI CCWWGG 2 cut(s) 193, 1184
FaqI GGGAC 1 cut(s) 835
FokI GGATG 7 cut(s) 62, 479, 722, 947, 1200, 1260, 1502
FspBI CTAG 5 cut(s) 356, 395, 602, 1304, 1334
GlaI GCGC 1 cut(s) 342
HaeIII GGCC 3 cut(s) 198, 224, 894
HhaI GCGC 1 cut(s) 343
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 3 cut(s) 408, 848, 1359
HphI GGTGA 3 cut(s) 241, 907, 1454
Hpy166II GTNNAC 3 cut(s) 17, 265, 369
Hpy188I TCNGA 8 cut(s) 83, 496, 770, 1105, 1145, 1197, 1425, 1563
Hpy188III TCNNGA 8 cut(s) 356, 412, 431, 586, 662, 671, 791, 1465
Hpy8I GTNNAC 3 cut(s) 17, 265, 369
HpyAV CCTTC 5 cut(s) 592, 641, 1386, 1453, 1543
HpyCH4III ACNGT 5 cut(s) 366, 376, 829, 1421, 1448
HpyCH4IV ACGT 2 cut(s) 773, 1127
HpyCH4V TGCA 8 cut(s) 170, 445, 637, 832, 865, 960, 1453, 1493
HpyF10VI GCNNNNNNNGC 3 cut(s) 551, 866, 871
HpyF3I CTNAG 4 cut(s) 80, 870, 1070, 1194
HpySE526I ACGT 2 cut(s) 773, 1127
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 3 cut(s) 30, 1372, 1413
LmnI GCTCC 2 cut(s) 771, 975
LweI GCATC 3 cut(s) 933, 969, 1480
MaeI CTAG 5 cut(s) 356, 395, 602, 1304, 1334
MaeII ACGT 2 cut(s) 773, 1127
MaeIII GTNAC 5 cut(s) 202, 297, 729, 1027, 1442
MalI GATC 3 cut(s) 32, 1374, 1415
MboI GATC 3 cut(s) 30, 1372, 1413
MboII GAAGA 7 cut(s) 176, 332, 442, 892, 1163, 1423, 1468
MfeI CAATTG 1 cut(s) 888
MhlI GDGCHC 1 cut(s) 1193
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 9 cut(s) 38, 481, 842, 888, 950, 1081, 1252, 1532, 1557
MluNI TGGCCA 1 cut(s) 224
MlyI GAGTC 2 cut(s) 857, 1353
MmeI TCCRAC 3 cut(s) 457, 1123, 1128
Mox20I TGGCCA 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 1557
MscI TGGCCA 1 cut(s) 224
MseI TTAA 4 cut(s) 41, 1161, 1235, 1380
MslI CAYNNNNRTG 3 cut(s) 374, 465, 1570
Msp20I TGGCCA 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 696
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 696
MvnI CGCG 2 cut(s) 343, 672
MwoI GCNNNNNNNGC 3 cut(s) 551, 866, 871
NcoI CCATGG 1 cut(s) 1184
NdeII GATC 3 cut(s) 30, 1372, 1413
NlaIV GGNNCC 3 cut(s) 180, 971, 1182
NmuCI GTSAC 3 cut(s) 297, 729, 1442
NruI TCGCGA 1 cut(s) 672
NspI RCATGY 1 cut(s) 449
OliI CACNNNNGTG 2 cut(s) 374, 1570
PagI TCATGA 2 cut(s) 661, 1464
PdmI GAANNNNTTC 1 cut(s) 1557
PfeI GAWTC 1 cut(s) 408
PleI GAGTC 2 cut(s) 856, 1353
PpsI GAGTC 2 cut(s) 856, 1353
Psp1406I AACGTT 1 cut(s) 773
Psp6I CCWGG 1 cut(s) 694
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 3 cut(s) 180, 971, 1182
PspPI GGNCC 2 cut(s) 628, 893
RruI TCGCGA 1 cut(s) 672
RsaI GTAC 3 cut(s) 368, 563, 1095
RsaNI GTAC 3 cut(s) 367, 562, 1094
RseI CAYNNNNRTG 3 cut(s) 374, 465, 1570
SaqAI TTAA 4 cut(s) 41, 1161, 1235, 1380
Sau3AI GATC 3 cut(s) 30, 1372, 1413
Sau96I GGNCC 2 cut(s) 628, 893
SchI GAGTC 2 cut(s) 857, 1353
ScrFI CCNGG 1 cut(s) 696
SduI GDGCHC 1 cut(s) 1193
SfaNI GCATC 3 cut(s) 933, 969, 1480
SfcI CTRYAG 1 cut(s) 1110
SinI GGWCC 1 cut(s) 628
SmiMI CAYNNNNRTG 3 cut(s) 374, 465, 1570
SmlI CTYRAG 3 cut(s) 293, 431, 1023
SmoI CTYRAG 3 cut(s) 293, 431, 1023
SpeI ACTAGT 1 cut(s) 1333
Sse9I AATT 9 cut(s) 38, 481, 842, 888, 950, 1081, 1252, 1532, 1557
SsiI CCGC 1 cut(s) 1273
SspMI CTAG 5 cut(s) 356, 395, 602, 1304, 1334
StyD4I CCNGG 1 cut(s) 694
StyI CCWWGG 2 cut(s) 193, 1184
TaaI ACNGT 5 cut(s) 366, 376, 829, 1421, 1448
TaiI ACGT 2 cut(s) 776, 1130
TaqI TCGA 1 cut(s) 420
TasI AATT 9 cut(s) 38, 481, 842, 888, 950, 1081, 1252, 1532, 1557
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 1 cut(s) 408
Tru1I TTAA 4 cut(s) 41, 1161, 1235, 1380
Tru9I TTAA 4 cut(s) 41, 1161, 1235, 1380
TscAI CASTG 3 cut(s) 381, 834, 1500
TseFI GTSAC 3 cut(s) 297, 729, 1442
Tsp45I GTSAC 3 cut(s) 297, 729, 1442
TspDTI ATGAA 8 cut(s) 49, 483, 991, 996, 1075, 1137, 1481, 1525
TspRI CASTG 3 cut(s) 381, 834, 1500
VpaK11BI GGWCC 1 cut(s) 628
XagI CCTNNNNNAGG 1 cut(s) 192
XapI RAATTY 6 cut(s) 481, 842, 950, 1081, 1252, 1557
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 1 cut(s) 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmnI GAANNNNTTC 1 cut(s) 1557
XspI CTAG 5 cut(s) 356, 395, 602, 1304, 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.