Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004277

Physical Location & Seq

Genomic Coordinates Forward (+)

29370384 .. 29373700

Gene Structure 3317 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1215200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGGAGGTGAAGAGGATCCATGCATTGTCGTTCGCGTTTCTGCTTCTCTTTGTTTCAGAATTCCTGTGTGGAATAGCGGTAGCAGCCGAAGATTCACCGAAGGTGGGGACGGTGATCGGAATCGATCTCGGAACGACGTATTCTTGTGTGGCGGTTTATAAGAACGGTCATGTGGAAATCATAGCGAACGATCAAGGCAACAGAATCACACCCTCCTGGGTCGCGTTCACTGATACGGAGCGTTTGATCGGAGAGGCGGCGAAGAACCAAGCCCCTCTCAACGCAGAACGCACCATTTTCGACGTCAAGCGGCTCATTGGGAGAAAGTTTGATGATCCAGAGGTGCAGAGAGATATTAAGTATTTACCCTATAAGGTTGTGAACAAAGATGGGAAGCCTTACATTCAGGTGAAGGTTAAAGGTGAAACCAAGGTTTTTAGCCCTGAGGAAATCAGTGCTATGATTTTGGGGAAGATGAAGGAAACCGCCGAGGCCTATCTTGGGAAGAAGATCAAAGATGCCGTGGTAACCGTTCCAGCGTATTTCAATGATGCGCAGAGGCAGGCTACAAAGGATGCAGGCATCATTGCCGGGCTCAATGTGGCTCGGATAATCAATGAGCCCACGGCCGCAGCCATTGCCTATGGTCTAGACAAGAAAGAAGAGAAGAACATACTGGTTTATGATCTTGGTGGTGGTACATTTGATGTTAGCATCTTGACCATTGACAATGGCGTGTTTGAGGTGAGGTCTACGAGTGGAGACACTCACTTGGGAGGAGAGGACTTCGACCATAGAGTGATGGACTACTTCATCAAGTTGATCAAGAAGAAATATAACAAAGATATTAGCAAGGACAACAAAGCTCTCGGGAAACTTCGAAAGGAATGTGAGAGAGCTAAGAGAGCGTTGAGTAGCCAGCATCAGGTTCGAGTGGAGATTGAATCTCTTTTTGACGGCATTGATTTTTCGGAGCCATTGACAAGGGCTAGATTCGAGGAGTTGAACATGGACTTGTTCAAGAAAACCCTGGGGCCAGTGAAAAAGGCTATGGAAGATGCAGGCTTGAAGAAGAAAGATATTAATGAGATTGTTCTGGTTGGAGGGAGTACCCGAATTCCTAAGGTGCAACAGATGTTGAAGGACTACTTCGATGGGAAAGAGCCAAGCAAGGGGATCAATCCTGATGAGGCTGTTGCATATGGTGCTGCAGTTCAAGGTGGAGTTCTCAGTGGCGAAGGCGGAGAAGAAACCAAAGATCTTCTTTTGCTTGATGTCGCTCCATTAAGTCTTGGTATCGAAACAGTTGGTGGCGTCATGACAAAATTGATTCCAAGGAACACTGTCATCCCGACGAAGAAGTCTCAAGTCTTCACCACTTATCAGGACCAGCAAACCACAGTAACCATCAAGGTGTACGAAGGTGAAAGAAGCTTGACGAAGGATTGTCGTGAGCTTGGAAAGTTTGATCTCACCGGCATTCCACCTGCTCCAAGGGGAGTGCCCCAAATCGAGGTGACATTTGAGGTCGATGCAAATGGCATACTACACGTGAGGGCTGAGGACAAGGCCGCGAAGAAGTCTCAATCCATAACGATCACAAATGACAAGGGGCGTCTAAGCCAGGAAGAAATAGAGAGAATGGTGAAGGAGGCTGAGGAGTTTGCCGAGGAGGATAACAAAGTGAGGGAAAAAATAGATGCCAGAAACAAACTTGAGACCTACATTTACAGCATGAAGAGCACTGTCAATGACAAGGACAAGCTGGCCGATAAGATTGATTCGGATGACAAGGAGAAGATCGAAAGCACGCTGAAGGAGGCGCTCGAGTGGCTGGATGACAACCAAAACGCCGAGAAGGATGATTTCGATGAGAAGCTGAAGGAGGTGGAGGCAGTGTGCAATCCGGTGATAAAGCAAGTGTATGAGAAGAGTGGTGGAAGTTCTGGTGGTTCACAGGATGAGGAAGAACCAAATGATGAGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005788 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016592 GO:0022414 GO:0030154 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044706 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0060560 GO:0070013 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 ko04918 ko05020 map03060 map04141 map04918 map05020

Pfam Domains

Protein Families

HSP70

Protein Analysis

663

Amino Acids

73.48

Weight (kDa)

5.2

Isoelectric Point (pI)

33.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	38 - 643	1.1e-266	Hsp70 protein
MreB_Mbl	PF06723	167 - 403	2.9e-18	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09080 AT1G09080
fragaria_vesca	FvH4_6g06470
malus_domestica	MD12G1215200.v1.1
prunus_persica	Prupe.6G321700_v2.0.a1
pyrus_communis	pycom04g17760 pycom12g20120
rosa_chinensis	RchiOBHm_Chr2g0142601 RchiOBHm_Chr3g0455591 RchiOBHm_Chr3g0455611 RchiOBHm_Chr3g0455631
rosa_laevigata	RLG00000019973 RLG00000019974 RLG00000025345 RLG00000025351
rosa_multiflora	Rmu_co8491437.1_g000001 Rmu_sc0000223.1_g000003 Rmu_sc0005164.1_g000002 Rmu_sc0015502.1_g000002
rosa_roxburghii	Rroxscaffold_2G00102460
rosa_rugosa	Rorug03G0008500 Rorug03G0008700
rosa_samantha	Rh2AG426400 Rh2BG436000 Rh2CG412900 Rh2DG445800 Rh3AG067800 Rh3AG068000 Rh3DG070300
rosa_wichuraiana	Rw2G034940 Rw3G005440 Rw3G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	159
AarI	CACCTGC	1 cut(s)	1495
AasI	GACNNNNNNGTC	1 cut(s)	1360
AatII	GACGTC	1 cut(s)	306
Acc16I	TGCGCA	1 cut(s)	555
Acc36I	ACCTGC	1 cut(s)	1495
AccI	GTMKAC	1 cut(s)	752
AccII	CGCG	3 cut(s)	35, 224, 1574
AciI	CCGC	8 cut(s)	77, 152, 257, 310, 486, 630, 1242, 1572
AclWI	GGATC	4 cut(s)	10, 23, 329, 1184
AcoI	YGGCCR	2 cut(s)	627, 1767
AcsI	RAATTY	2 cut(s)	59, 1116
AcuI	CTGAAG	2 cut(s)	1835, 1901
AcvI	CACGTG	1 cut(s)	1552
AcyI	GRCGYC	3 cut(s)	303, 1314, 1615
AfaI	GTAC	3 cut(s)	700, 1111, 1418
AfiI	CCNNNNNNNGG	5 cut(s)	104, 501, 925, 1172, 1513
AflIII	ACRYGT	1 cut(s)	1549
AgsI	TTSAA	7 cut(s)	547, 944, 1006, 1021, 1069, 1141, 1217
AjnI	CCWGG	3 cut(s)	215, 1029, 1623
AluBI	AGCT	7 cut(s)	866, 899, 1434, 1456, 1765, 1879, 1984
AluI	AGCT	7 cut(s)	866, 899, 1434, 1456, 1765, 1879, 1984
Alw21I	GWGCWC	1 cut(s)	1745
Alw26I	GTCTC	4 cut(s)	756, 1368, 1587, 1712
AlwI	GGATC	4 cut(s)	10, 23, 329, 1184
Ama87I	CYCGRG	2 cut(s)	869, 1826
AoxI	GGCC	5 cut(s)	492, 627, 1034, 1569, 1767
ApeKI	GCWGC	3 cut(s)	83, 632, 1208
ApoI	RAATTY	2 cut(s)	59, 1116
AseI	ATTAAT	1 cut(s)	1083
AspLEI	GCGC	2 cut(s)	556, 1825
AspS9I	GGNCC	2 cut(s)	1034, 1387
AsuC2I	CCSGG	1 cut(s)	592
AsuII	TTCGAA	1 cut(s)	880
AvaI	CYCGRG	2 cut(s)	869, 1826
AvaII	GGWCC	1 cut(s)	1387
AxyI	CCTNAGG	2 cut(s)	444, 1122
BaeGI	GKGCMC	1 cut(s)	1506
BamHI	GGATCC	1 cut(s)	15
BanII	GRGCYC	2 cut(s)	597, 624
BbrPI	CACGTG	1 cut(s)	1552
BbsI	GAAGAC	1 cut(s)	1363
Bbv12I	GWGCWC	1 cut(s)	1745
BbvCI	CCTCAGC	2 cut(s)	1560, 1656
BbvI	GCAGC	3 cut(s)	95, 644, 1195
BccI	CCATC	4 cut(s)	383, 796, 1148, 1415
BceAI	ACGGC	3 cut(s)	506, 642, 973
BcgI	CGANNNNNNTGC	4 cut(s)	280, 314, 911, 945
BciT130I	CCWGG	3 cut(s)	217, 1031, 1625
BclI	TGATCA	1 cut(s)	822
BcnI	CCSGG	1 cut(s)	592
BcoDI	GTCTC	4 cut(s)	756, 1368, 1587, 1712
BfaI	CTAG	2 cut(s)	650, 990
BfmI	CTRYAG	2 cut(s)	1209, 1985
BfoI	RGCGCY	1 cut(s)	1826
BfuAI	ACCTGC	1 cut(s)	1495
BglII	AGATCT	1 cut(s)	1258
BisI	GCNGC	7 cut(s)	84, 258, 311, 630, 633, 1209, 1572
BlsI	GCNGC	7 cut(s)	85, 259, 312, 631, 634, 1210, 1573
Bme1390I	CCNGG	4 cut(s)	217, 592, 1031, 1625
Bme18I	GGWCC	1 cut(s)	1387
BmeT110I	CYCGRG	2 cut(s)	869, 1826
BmgT120I	GGNCC	2 cut(s)	1034, 1387
BmiI	GGNNCC	3 cut(s)	17, 975, 1035
BmrFI	CCNGG	4 cut(s)	217, 592, 1031, 1625
BmsI	GCATC	9 cut(s)	508, 541, 565, 591, 723, 931, 1048, 1522, 1690
BpiI	GAAGAC	1 cut(s)	1363
Bpu10I	CCTNAGC	2 cut(s)	1560, 1656
Bpu14I	TTCGAA	1 cut(s)	880
BpuEI	CTTGAG	2 cut(s)	1350, 1736
BpuMI	CCSGG	1 cut(s)	592
Bsa29I	ATCGAT	1 cut(s)	123
BsaAI	YACGTR	1 cut(s)	1552
BsaBI	GATNNNNATC	1 cut(s)	119
BsaHI	GRCGYC	3 cut(s)	303, 1314, 1615
BsaI	GGTCTC	1 cut(s)	1712
BsaWI	WCCGGW	1 cut(s)	1906
BsaXI	ACNNNNNCTCC	2 cut(s)	1883, 1913
Bsc4I	CCNNNNNNNGG	5 cut(s)	104, 501, 925, 1172, 1513
Bse118I	RCCGGY	1 cut(s)	1475
Bse1I	ACTGG	2 cut(s)	681, 1037
Bse21I	CCTNAGG	2 cut(s)	444, 1122
Bse3DI	GCAATG	2 cut(s)	585, 636
Bse8I	GATNNNNATC	1 cut(s)	119
BseBI	CCWGG	3 cut(s)	217, 1031, 1625
BseCI	ATCGAT	1 cut(s)	123
BseGI	GGATG	6 cut(s)	580, 1347, 1792, 1843, 1867, 1966
BseJI	GATNNNNATC	1 cut(s)	119
BseLI	CCNNNNNNNGG	5 cut(s)	104, 501, 925, 1172, 1513
BseMI	GCAATG	2 cut(s)	585, 636
BseMII	CTCAG	4 cut(s)	435, 1243, 1551, 1647
BseNI	ACTGG	2 cut(s)	681, 1037
BseRI	GAGGAG	4 cut(s)	792, 1013, 1673, 1685
BseSI	GKGCMC	1 cut(s)	1506
BseX3I	CGGCCG	1 cut(s)	627
BseXI	GCAGC	3 cut(s)	95, 644, 1195
BsgI	GTGCAG	1 cut(s)	365
Bsh1236I	CGCG	3 cut(s)	35, 224, 1574
Bsh1285I	CGRYCG	1 cut(s)	630
BshFI	GGCC	5 cut(s)	494, 629, 1036, 1571, 1769
BshVI	ATCGAT	1 cut(s)	123
BsiEI	CGRYCG	1 cut(s)	630
BsiHKAI	GWGCWC	1 cut(s)	1745
BsiHKCI	CYCGRG	2 cut(s)	869, 1826
BsiSI	CCGG	3 cut(s)	591, 1476, 1907
BslFI	GGGAC	1 cut(s)	121
BslI	CCNNNNNNNGG	5 cut(s)	104, 501, 925, 1172, 1513
BsmAI	GTCTC	4 cut(s)	756, 1368, 1587, 1712
BsmFI	GGGAC	1 cut(s)	121
BsmI	GAATGC	1 cut(s)	1479
BsnI	GGCC	5 cut(s)	494, 629, 1036, 1571, 1769
Bso31I	GGTCTC	1 cut(s)	1712
BsoBI	CYCGRG	2 cut(s)	869, 1826
Bsp119I	TTCGAA	1 cut(s)	880
Bsp1286I	GDGCHC	4 cut(s)	597, 624, 1506, 1745
BspACI	CCGC	8 cut(s)	77, 152, 257, 310, 486, 630, 1242, 1572
BspANI	GGCC	5 cut(s)	494, 629, 1036, 1571, 1769
BspCNI	CTCAG	4 cut(s)	436, 1242, 1552, 1648
BspDI	ATCGAT	1 cut(s)	123
BspFNI	CGCG	3 cut(s)	35, 224, 1574
BspHI	TCATGA	1 cut(s)	1317
BspLI	GGNNCC	3 cut(s)	17, 975, 1035
BspMAI	CTGCAG	1 cut(s)	1213
BspMI	ACCTGC	1 cut(s)	1495
BspPI	GGATC	4 cut(s)	10, 23, 329, 1184
BspQI	GCTCTTC	1 cut(s)	1733
BspT104I	TTCGAA	1 cut(s)	880
BspTNI	GGTCTC	1 cut(s)	1712
BsrDI	GCAATG	2 cut(s)	585, 636
BsrFI	RCCGGY	1 cut(s)	1475
BsrI	ACTGG	2 cut(s)	681, 1037
BssAI	RCCGGY	1 cut(s)	1475
BssNI	GRCGYC	3 cut(s)	303, 1314, 1615
BssT1I	CCWWGG	3 cut(s)	429, 1334, 1493
Bst2UI	CCWGG	3 cut(s)	217, 1031, 1625
Bst4CI	ACNGT	7 cut(s)	112, 167, 532, 1306, 1345, 1402, 1747
Bst6I	CTCTTC	4 cut(s)	5, 657, 1733, 1925
BstACI	GRCGYC	3 cut(s)	303, 1314, 1615
BstAPI	GCANNNNNTGC	2 cut(s)	638, 1205
BstBAI	YACGTR	1 cut(s)	1552
BstBI	TTCGAA	1 cut(s)	880
BstC8I	GCNNGC	6 cut(s)	564, 580, 920, 1063, 1767, 1811
BstDEI	CTNAG	7 cut(s)	444, 900, 1122, 1229, 1560, 1619, 1656
BstDSI	CCRYGG	2 cut(s)	522, 624
BstEII	GGTNACC	1 cut(s)	526
BstF5I	GGATG	6 cut(s)	580, 1347, 1792, 1843, 1867, 1966
BstFNI	CGCG	3 cut(s)	35, 224, 1574
BstH2I	RGCGCY	1 cut(s)	1826
BstHHI	GCGC	2 cut(s)	556, 1825
BstMAI	GTCTC	4 cut(s)	756, 1368, 1587, 1712
BstMCI	CGRYCG	1 cut(s)	630
BstMWI	GCNNNNNNNGC	6 cut(s)	83, 638, 905, 1205, 1740, 1831
BstNI	CCWGG	3 cut(s)	217, 1031, 1625
BstPI	GGTNACC	1 cut(s)	526
BstSCI	CCNGG	4 cut(s)	215, 590, 1029, 1623
BstSFI	CTRYAG	2 cut(s)	1209, 1985
BstSLI	GKGCMC	1 cut(s)	1506
BstUI	CGCG	3 cut(s)	35, 224, 1574
BstV1I	GCAGC	3 cut(s)	95, 644, 1195
BstV2I	GAAGAC	1 cut(s)	1363
BstX2I	RGATCY	2 cut(s)	15, 1258
BstYI	RGATCY	2 cut(s)	15, 1258
BstZI	CGGCCG	1 cut(s)	627
Bsu15I	ATCGAT	1 cut(s)	123
Bsu36I	CCTNAGG	2 cut(s)	444, 1122
BsuRI	GGCC	5 cut(s)	494, 629, 1036, 1571, 1769
BsuTUI	ATCGAT	1 cut(s)	123
BtgI	CCRYGG	2 cut(s)	522, 624
BtsCI	GGATG	6 cut(s)	580, 1347, 1792, 1843, 1867, 1966
BtsI	GCAGTG	1 cut(s)	1902
BtsIMutI	CAGTG	7 cut(s)	228, 460, 1044, 1237, 1341, 1743, 1902
BveI	ACCTGC	1 cut(s)	1495
Cac8I	GCNNGC	6 cut(s)	564, 580, 920, 1063, 1767, 1811
CciI	TCATGA	1 cut(s)	1317
CfoI	GCGC	2 cut(s)	556, 1825
Cfr10I	RCCGGY	1 cut(s)	1475
Cfr13I	GGNCC	2 cut(s)	1034, 1387
ClaI	ATCGAT	1 cut(s)	123
CseI	GACGC	2 cut(s)	1303, 1604
Csp6I	GTAC	3 cut(s)	699, 1110, 1417
CviAII	CATG	5 cut(s)	20, 170, 1009, 1318, 1735
CviQI	GTAC	3 cut(s)	699, 1110, 1417
DdeI	CTNAG	7 cut(s)	444, 900, 1122, 1229, 1560, 1619, 1656
DrdI	GACNNNNNNGTC	1 cut(s)	1360
DseDI	GACNNNNNNGTC	1 cut(s)	1360
EaeI	YGGCCR	2 cut(s)	627, 1767
EagI	CGGCCG	1 cut(s)	627
Eam1104I	CTCTTC	4 cut(s)	5, 657, 1733, 1925
EarI	CTCTTC	4 cut(s)	5, 657, 1733, 1925
EciI	GGCGGA	1 cut(s)	1257
EclXI	CGGCCG	1 cut(s)	627
Eco130I	CCWWGG	3 cut(s)	429, 1334, 1493
Eco147I	AGGCCT	1 cut(s)	494
Eco24I	GRGCYC	2 cut(s)	597, 624
Eco31I	GGTCTC	1 cut(s)	1712
Eco47I	GGWCC	1 cut(s)	1387
Eco52I	CGGCCG	1 cut(s)	627
Eco57I	CTGAAG	2 cut(s)	1835, 1901
Eco72I	CACGTG	1 cut(s)	1552
Eco81I	CCTNAGG	2 cut(s)	444, 1122
Eco88I	CYCGRG	2 cut(s)	869, 1826
Eco91I	GGTNACC	1 cut(s)	526
EcoO65I	GGTNACC	1 cut(s)	526
EcoRI	GAATTC	2 cut(s)	59, 1116
EcoRII	CCWGG	3 cut(s)	215, 1029, 1623
EcoT14I	CCWWGG	3 cut(s)	429, 1334, 1493
EcoT22I	ATGCAT	1 cut(s)	25
EcoT38I	GRGCYC	2 cut(s)	597, 624
ErhI	CCWWGG	3 cut(s)	429, 1334, 1493
FaeI	CATG	5 cut(s)	23, 173, 1012, 1321, 1738
FalI	AAGNNNNNCTT	4 cut(s)	1133, 1165, 1248, 1280
FaqI	GGGAC	1 cut(s)	121
FatI	CATG	5 cut(s)	19, 169, 1008, 1317, 1734
FauNDI	CATATG	1 cut(s)	1201
FbaI	TGATCA	1 cut(s)	822
FblI	GTMKAC	1 cut(s)	752
Fnu4HI	GCNGC	7 cut(s)	84, 258, 311, 630, 633, 1209, 1572
FokI	GGATG	6 cut(s)	587, 1334, 1799, 1850, 1874, 1973
FriOI	GRGCYC	2 cut(s)	597, 624
Fsp4HI	GCNGC	7 cut(s)	84, 258, 311, 630, 633, 1209, 1572
FspBI	CTAG	2 cut(s)	650, 990
FspI	TGCGCA	1 cut(s)	555
GlaI	GCGC	2 cut(s)	555, 1824
GluI	GCNGC	7 cut(s)	84, 258, 311, 630, 633, 1209, 1572
HaeII	RGCGCY	1 cut(s)	1826
HaeIII	GGCC	5 cut(s)	494, 629, 1036, 1571, 1769
HapII	CCGG	3 cut(s)	591, 1476, 1907
HgaI	GACGC	2 cut(s)	1303, 1604
HhaI	GCGC	2 cut(s)	556, 1825
Hin1I	GRCGYC	3 cut(s)	303, 1314, 1615
Hin1II	CATG	5 cut(s)	23, 173, 1012, 1321, 1738
Hin6I	GCGC	2 cut(s)	554, 1823
HinP1I	GCGC	2 cut(s)	554, 1823
HindIII	AAGCTT	1 cut(s)	1432
HinfI	GANTC	7 cut(s)	92, 120, 204, 944, 993, 1330, 1781
HpaII	CCGG	3 cut(s)	591, 1476, 1907
Hpy166II	GTNNAC	5 cut(s)	228, 382, 753, 1417, 1955
Hpy188I	TCNGA	7 cut(s)	58, 119, 131, 251, 609, 973, 1786
Hpy8I	GTNNAC	5 cut(s)	228, 382, 753, 1417, 1955
Hpy99I	CGWCG	3 cut(s)	139, 305, 1357
HpyCH4III	ACNGT	7 cut(s)	112, 167, 532, 1306, 1345, 1402, 1747
HpyCH4IV	ACGT	3 cut(s)	137, 303, 1551
HpyCH4V	TGCA	9 cut(s)	23, 346, 578, 1061, 1129, 1199, 1211, 1535, 1902
HpyF10VI	GCNNNNNNNGC	6 cut(s)	83, 638, 905, 1205, 1740, 1831
HpyF3I	CTNAG	7 cut(s)	444, 900, 1122, 1229, 1560, 1619, 1656
HpySE526I	ACGT	3 cut(s)	137, 303, 1551
Hsp92I	GRCGYC	3 cut(s)	303, 1314, 1615
Hsp92II	CATG	5 cut(s)	23, 173, 1012, 1321, 1738
HspAI	GCGC	2 cut(s)	554, 1823
Ksp22I	TGATCA	1 cut(s)	822
LguI	GCTCTTC	1 cut(s)	1733
LmnI	GCTCC	4 cut(s)	238, 973, 1285, 1495
Lsp1109I	GCAGC	3 cut(s)	95, 644, 1195
LweI	GCATC	9 cut(s)	508, 541, 565, 591, 723, 931, 1048, 1522, 1690
MaeI	CTAG	2 cut(s)	650, 990
MaeII	ACGT	3 cut(s)	137, 303, 1551
MaeIII	GTNAC	3 cut(s)	526, 1402, 1516
MflI	RGATCY	2 cut(s)	15, 1258
MhlI	GDGCHC	4 cut(s)	597, 624, 1506, 1745
MluCI	AATT	3 cut(s)	59, 1116, 1325
MmeI	TCCRAC	1 cut(s)	1081
Mph1103I	ATGCAT	1 cut(s)	25
MseI	TTAA	4 cut(s)	357, 417, 1083, 1286
MslI	CAYNNNNRTG	2 cut(s)	407, 1412
MspI	CCGG	3 cut(s)	591, 1476, 1907
MspR9I	CCNGG	4 cut(s)	217, 592, 1031, 1625
Mva1269I	GAATGC	1 cut(s)	1479
MvaI	CCWGG	3 cut(s)	217, 1031, 1625
MvnI	CGCG	3 cut(s)	35, 224, 1574
MwoI	GCNNNNNNNGC	6 cut(s)	83, 638, 905, 1205, 1740, 1831
NciI	CCSGG	1 cut(s)	592
NdeI	CATATG	1 cut(s)	1201
NlaIII	CATG	5 cut(s)	23, 173, 1012, 1321, 1738
NlaIV	GGNNCC	3 cut(s)	17, 975, 1035
NmeAIII	GCCGAG	3 cut(s)	514, 1693, 1879
NmuCI	GTSAC	1 cut(s)	1516
NsbI	TGCGCA	1 cut(s)	555
NsiI	ATGCAT	1 cut(s)	25
NspV	TTCGAA	1 cut(s)	880
PaeR7I	CTCGAG	1 cut(s)	1826
PagI	TCATGA	1 cut(s)	1317
PaqCI	CACCTGC	1 cut(s)	1495
PasI	CCCWGGG	1 cut(s)	1030
PceI	AGGCCT	1 cut(s)	494
PciSI	GCTCTTC	1 cut(s)	1733
PctI	GAATGC	1 cut(s)	1479
PfeI	GAWTC	7 cut(s)	92, 120, 204, 944, 993, 1330, 1781
PkrI	GCNGC	7 cut(s)	85, 259, 312, 631, 634, 1210, 1573
PmaCI	CACGTG	1 cut(s)	1552
PmlI	CACGTG	1 cut(s)	1552
Ppu21I	YACGTR	1 cut(s)	1552
PshBI	ATTAAT	1 cut(s)	1083
PsiI	TTATAA	1 cut(s)	159
Psp6I	CCWGG	3 cut(s)	215, 1029, 1623
PspCI	CACGTG	1 cut(s)	1552
PspEI	GGTNACC	1 cut(s)	526
PspGI	CCWGG	3 cut(s)	215, 1029, 1623
PspN4I	GGNNCC	3 cut(s)	17, 975, 1035
PspPI	GGNCC	2 cut(s)	1034, 1387
PspXI	VCTCGAGB	1 cut(s)	1826
PstI	CTGCAG	1 cut(s)	1213
PsuI	RGATCY	2 cut(s)	15, 1258
RsaI	GTAC	3 cut(s)	700, 1111, 1418
RsaNI	GTAC	3 cut(s)	699, 1110, 1417
RseI	CAYNNNNRTG	2 cut(s)	407, 1412
SapI	GCTCTTC	1 cut(s)	1733
SaqAI	TTAA	4 cut(s)	357, 417, 1083, 1286
SatI	GCNGC	7 cut(s)	84, 258, 311, 630, 633, 1209, 1572
Sau96I	GGNCC	2 cut(s)	1034, 1387
ScrFI	CCNGG	4 cut(s)	217, 592, 1031, 1625
SduI	GDGCHC	4 cut(s)	597, 624, 1506, 1745
SfaNI	GCATC	9 cut(s)	508, 541, 565, 591, 723, 931, 1048, 1522, 1690
SfcI	CTRYAG	2 cut(s)	1209, 1985
Sfr274I	CTCGAG	1 cut(s)	1826
SfuI	TTCGAA	1 cut(s)	880
SinI	GGWCC	1 cut(s)	1387
SlaI	CTCGAG	1 cut(s)	1826
SmiMI	CAYNNNNRTG	2 cut(s)	407, 1412
SmlI	CTYRAG	3 cut(s)	1365, 1715, 1826
SmoI	CTYRAG	3 cut(s)	1365, 1715, 1826
Sse9I	AATT	3 cut(s)	59, 1116, 1325
SseBI	AGGCCT	1 cut(s)	494
SsiI	CCGC	8 cut(s)	77, 152, 257, 310, 486, 630, 1242, 1572
SspMI	CTAG	2 cut(s)	650, 990
StuI	AGGCCT	1 cut(s)	494
StyD4I	CCNGG	4 cut(s)	215, 590, 1029, 1623
StyI	CCWWGG	3 cut(s)	429, 1334, 1493
TaaI	ACNGT	7 cut(s)	112, 167, 532, 1306, 1345, 1402, 1747
TaiI	ACGT	3 cut(s)	140, 306, 1554
TasI	AATT	3 cut(s)	59, 1116, 1325
TauI	GCSGC	4 cut(s)	260, 313, 632, 1574
TfiI	GAWTC	7 cut(s)	92, 120, 204, 944, 993, 1330, 1781
Tru1I	TTAA	4 cut(s)	357, 417, 1083, 1286
Tru9I	TTAA	4 cut(s)	357, 417, 1083, 1286
TscAI	CASTG	7 cut(s)	235, 460, 1044, 1237, 1348, 1750, 1902
TseFI	GTSAC	1 cut(s)	1516
TseI	GCWGC	3 cut(s)	83, 632, 1208
Tsp45I	GTSAC	1 cut(s)	1516
TspDTI	ATGAA	3 cut(s)	491, 802, 1751
TspGWI	ACGGA	1 cut(s)	251
TspRI	CASTG	7 cut(s)	235, 460, 1044, 1237, 1348, 1750, 1902
VpaK11BI	GGWCC	1 cut(s)	1387
VspI	ATTAAT	1 cut(s)	1083
XapI	RAATTY	2 cut(s)	59, 1116
XbaI	TCTAGA	1 cut(s)	649
XhoI	CTCGAG	1 cut(s)	1826
XmiI	GTMKAC	1 cut(s)	752
XspI	CTAG	2 cut(s)	650, 990
ZraI	GACGTC	1 cut(s)	304
Zsp2I	ATGCAT	1 cut(s)	25

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.