RLG00000019974
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004277
Physical Location & Seq
Reverse (-)
59443230 .. 59445076
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019974

Sequence Viewer

Length: 711 bp
ATGGCGTTGAAGATGGCGATTCTGCTTTTCTTTATCTTGGTCACAGGAGCTTCGTCTGGAATGGCATTCACCTGTGGAATCAATGCTGCAATGTTCTTGAAGATGCAGGAAACAGCTGAGGGGCAGGCTGCTCAGGATGCAAGCACCATTGCTGAGCATATTGAAAAAAAGGATATACTGGTTTATAATCTTGGAGGGGGTGCATTAGATGTGATAATGGCGACAAGTGGAGTCATCCACTTGGGAGGTAGGATCTTTGACGACGGACTGGTTGACTTTTTCATCACATTGATCAAGAGGAAGTACTACCGTGATATCCGCAAAAATAACAAGGCTGTTGCCAAGCTTCGCAAGGATTGTGAGAGAGCAAAGGGAGCCTTGAGTAGACAGCACCAAACCCATGTGGAGGTTGAGTCTCTAGTTGATGGTATTGATTATTTCTCGGAGCCATTGACCAGGGCTAAGTTTGAGGAGTTGAACATGGAGTTGTTTAACAAGACCATCGGGACTATGACAAATTATATTCAAGAGATTGTGCTTGTTGGAGGGAGTACTCGAATTCCTAAGGTGCAACAGTTGTTGAAGGACTTCTTCAACGGGAAAGAACCAAACAAGGGTGTTAATCCTGATGAGGCCATTGCCTTCGGTGCTGCAGTTCTGGGTGGAGCCTTTAGTGGAGACAGCTGGAATGAAACCAAAGGTATTGATTGA

Protein Analysis

237

Amino Acids

25.86

Weight (kDa)

6.12

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 55 - 227 2.5e-47 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
AccI GTMKAC 1 cut(s) 385
AciI CCGC 1 cut(s) 319
AclWI GGATC 1 cut(s) 260
AcsI RAATTY 1 cut(s) 558
AfaI GTAC 2 cut(s) 305, 553
AfiI CCNNNNNNNGG 2 cut(s) 406, 614
AgsI TTSAA 7 cut(s) 10, 100, 164, 478, 527, 583, 595
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 4 cut(s) 50, 116, 346, 684
AluI AGCT 4 cut(s) 50, 116, 346, 684
Alw26I GTCTC 2 cut(s) 420, 672
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 633
ApeKI GCWGC 3 cut(s) 86, 128, 650
ApoI RAATTY 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 61
AxyI CCTNAGG 1 cut(s) 564
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 73, 115, 637
BccI CCATC 3 cut(s) 7, 419, 509
BciT130I CCWGG 1 cut(s) 457
BclI TGATCA 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 420, 672
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 651
BisI GCNGC 3 cut(s) 87, 129, 651
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 3 cut(s) 88, 130, 652
BmcAI AGTACT 2 cut(s) 305, 553
Bme1390I CCNGG 1 cut(s) 457
BmiI GGNNCC 3 cut(s) 376, 447, 667
BmrFI CCNGG 1 cut(s) 457
BmsI GCATC 2 cut(s) 93, 127
Bpu10I CCTNAGC 2 cut(s) 117, 132
Bpu1102I GCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 614
Bse1I ACTGG 2 cut(s) 183, 273
Bse21I CCTNAGG 1 cut(s) 564
Bse3DI GCAATG 3 cut(s) 96, 147, 636
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 2 cut(s) 142, 234
BseLI CCNNNNNNNGG 2 cut(s) 406, 614
BseMI GCAATG 3 cut(s) 96, 147, 636
BseMII CTCAG 3 cut(s) 108, 144, 146
BseNI ACTGG 2 cut(s) 183, 273
BseRI GAGGAG 1 cut(s) 485
BseXI GCAGC 3 cut(s) 73, 115, 637
BshFI GGCC 1 cut(s) 635
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 2 cut(s) 406, 614
BsmAI GTCTC 2 cut(s) 420, 672
BsmFI GGGAC 1 cut(s) 520
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 1 cut(s) 635
Bsp143I GATC 2 cut(s) 252, 291
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 1 cut(s) 319
BspANI GGCC 1 cut(s) 635
BspCNI CTCAG 3 cut(s) 109, 145, 145
BspLI GGNNCC 3 cut(s) 376, 447, 667
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 1 cut(s) 260
BsrDI GCAATG 3 cut(s) 96, 147, 636
BsrI ACTGG 2 cut(s) 183, 273
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 2 cut(s) 252, 291
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 2 cut(s) 311, 576
BstC8I GCNNGC 2 cut(s) 126, 142
BstDEI CTNAG 5 cut(s) 117, 132, 153, 462, 564
BstF5I GGATG 2 cut(s) 142, 234
BstKTI GATC 2 cut(s) 255, 294
BstMAI GTCTC 2 cut(s) 420, 672
BstMBI GATC 2 cut(s) 252, 291
BstMWI GCNNNNNNNGC 3 cut(s) 137, 374, 647
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 455
BstSFI CTRYAG 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 73, 115, 637
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
Bsu36I CCTNAGG 1 cut(s) 564
BsuRI GGCC 1 cut(s) 635
BtsCI GGATG 2 cut(s) 142, 234
Cac8I GCNNGC 2 cut(s) 126, 142
Csp6I GTAC 2 cut(s) 304, 552
CviAII CATG 2 cut(s) 401, 481
CviQI GTAC 2 cut(s) 304, 552
DdeI CTNAG 5 cut(s) 117, 132, 153, 462, 564
DpnI GATC 2 cut(s) 254, 293
DpnII GATC 2 cut(s) 252, 291
Eco32I GATATC 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 455
EcoRV GATATC 1 cut(s) 316
FaeI CATG 2 cut(s) 404, 484
FaiI YATR 7 cut(s) 159, 176, 186, 402, 482, 512, 522
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 575, 607
FaqI GGGAC 1 cut(s) 520
FatI CATG 2 cut(s) 400, 480
FbaI TGATCA 1 cut(s) 291
FblI GTMKAC 1 cut(s) 385
Fnu4HI GCNGC 3 cut(s) 87, 129, 651
FokI GGATG 2 cut(s) 149, 221
Fsp4HI GCNGC 3 cut(s) 87, 129, 651
FspBI CTAG 1 cut(s) 419
GluI GCNGC 3 cut(s) 87, 129, 651
HaeIII GGCC 1 cut(s) 635
Hin1II CATG 2 cut(s) 404, 484
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 4 cut(s) 19, 78, 231, 413
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 274, 386
Hpy188I TCNGA 1 cut(s) 445
Hpy188III TCNNGA 7 cut(s) 57, 97, 134, 295, 505, 527, 626
Hpy8I GTNNAC 2 cut(s) 274, 386
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 577, 652
HpyCH4III ACNGT 2 cut(s) 311, 576
HpyCH4V TGCA 6 cut(s) 89, 106, 140, 203, 571, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 374, 647
HpyF3I CTNAG 5 cut(s) 117, 132, 153, 462, 564
Hsp92II CATG 2 cut(s) 404, 484
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 2 cut(s) 252, 291
LmnI GCTCC 4 cut(s) 47, 374, 445, 665
Lsp1109I GCAGC 3 cut(s) 73, 115, 637
LweI GCATC 2 cut(s) 93, 127
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 40
MalI GATC 2 cut(s) 254, 293
MboI GATC 2 cut(s) 252, 291
MboII GAAGA 3 cut(s) 22, 112, 583
MflI RGATCY 1 cut(s) 252
MluCI AATT 2 cut(s) 517, 558
MlyI GAGTC 2 cut(s) 240, 422
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 8 cut(s) 112, 188, 239, 291, 400, 463, 539, 625
MroXI GAANNNNTTC 1 cut(s) 587
MseI TTAA 2 cut(s) 492, 621
MspA1I CMGCKG 2 cut(s) 116, 684
MspR9I CCNGG 1 cut(s) 457
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 1 cut(s) 457
MwoI GCNNNNNNNGC 3 cut(s) 137, 374, 647
NdeII GATC 2 cut(s) 252, 291
NlaIII CATG 2 cut(s) 404, 484
NlaIV GGNNCC 3 cut(s) 376, 447, 667
NmuCI GTSAC 1 cut(s) 40
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 19, 78
PkrI GCNGC 3 cut(s) 88, 130, 652
PleI GAGTC 2 cut(s) 239, 421
PpsI GAGTC 2 cut(s) 239, 421
PsiI TTATAA 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 3 cut(s) 376, 447, 667
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 252
PvuII CAGCTG 2 cut(s) 116, 684
RsaI GTAC 2 cut(s) 305, 553
RsaNI GTAC 2 cut(s) 304, 552
SaqAI TTAA 2 cut(s) 492, 621
SatI GCNGC 3 cut(s) 87, 129, 651
Sau3AI GATC 2 cut(s) 252, 291
ScaI AGTACT 2 cut(s) 305, 553
SchI GAGTC 2 cut(s) 240, 422
ScrFI CCNGG 1 cut(s) 457
SetI ASST 9 cut(s) 52, 74, 118, 250, 348, 411, 570, 686, 703
SfaNI GCATC 2 cut(s) 93, 127
SfcI CTRYAG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 2 cut(s) 517, 558
SsiI CCGC 1 cut(s) 319
SspMI CTAG 1 cut(s) 419
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 2 cut(s) 311, 576
TaqI TCGA 1 cut(s) 556
TasI AATT 2 cut(s) 517, 558
TatI WGTACW 2 cut(s) 303, 551
TfiI GAWTC 2 cut(s) 19, 78
Tru1I TTAA 2 cut(s) 492, 621
Tru9I TTAA 2 cut(s) 492, 621
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 3 cut(s) 86, 128, 650
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 271, 705
TspGWI ACGGA 1 cut(s) 279
XapI RAATTY 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 1 cut(s) 419
ZrmI AGTACT 2 cut(s) 305, 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.