Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004277

Physical Location & Seq

Genomic Coordinates Forward (+)

21753116 .. 21755021

Gene Structure 1906 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g20120.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGATCAAAGACGCCGTGGTAACCGTTCCAGCGTATTTCAATGATGCGCAGAGGCAGGCTACCAAGGATGCAGGCATCATTGCCGGGCTCAATGTGGCTCGGATAATCAATGAGCCCACGGCCGCAGCCATTGCCTATGGTCTAGACAAGAAAGGAGAGAAGAACATACTGGTTTATGATCTTGGTGGCGGTACATTTGATGTTAGCATCTTGACCATTGACAATGGCGTGTTTGAGGTGAGGTCTACAAGTGGAGACACTCACTTGGGAGGAGAGGACTTTGACCATAGAGTGATGGACTACTTCATCAAGTTGATCAAGAAGAAATATAACAAAGATATTAGCAAGGACAACAAAGCTCTTGGGAAACTTCGAAAGGAATGTGAGAGAGCTAAGAGAGCGTTGAGTAGCCAGCATCAGGTTCGAGTGGAGATTGAATCTCTTTTTGACGGCATTGATTTTTCGGAGCCATTGACAAGGGCTAGATTCGAGGAGTTGAATATGGACTTGTTCAAGAAAACCATGGGGCCGGTGAAAAAGGCTATGGAAGATGCAGGCTTGAAGAAGAAAGATATTCACGAGATTGTCCTGGTTGGAGGGAGTACCCGAATCCCTAAGGTGCAACAGATGTTGAAGGACTACTTCGATGGGAAAGAGCCAAGCAAGGGGATCAATCCTGATGAGGCTGTTGCATATGGTGCTGCAGTTCAAGGTGGAGTTCTCAGTGGCGAAGGCGGAGAAGAAACCAAAGATCTTCTTTTGCTTGATGTCGCTCCATTGAGTCTTGGTATCGAAACAGTTGGTGGTGTCATGACAAAATTGATTCCAAGGAACACTGTCATCCCGACAAAGAAGTCTCAAGTCTTCACCACTTATCAGGACCAGCAAACCACAGTAACCATCAAGGTGTACGAAGGTGAAAGAAGCTTGACGAAGGATTGTCGTGAGCTTGGAAAGTTTGATCTCACCGGTATTCCGCCTGCTCCAAGGGGAGTGCCCCAAATTGAGGTGACATTTGAGGTCGATGCAAATGGCATACTACACGTAAGGGCTGAGGACAAGGCCGCGAAGAAGTCTCAATCCATAACGATCACAAATGACAAAGGGCGTCTAAGCCAGGAAGAAATAGAGAGAATGGTGAAGGAGGCTGAGGAGTTTGCCGAGGAGGATAACAAAGTGAGGGAAAAAATAGATGCCAGGAACAAACTTGAGACCTACATTTACAGCATGAAGAGCACTGTCAATGACAAGGACAAGCTGGCGGATAAGATTGATTCGGATGACAAGGAGAAGATCGAAAGCACGCTAAAGGAGGCGCTCGAGTGGCTGGATGACAACCAAAACGCCGATAAGGATGATTTCGACGAGAAGCTGAAGGAGGTGGAGGCAGTGTGCAATCCGGTGATAAAGCAAGTGTATGAGAAGAGCGGTGGAAGTTCTGGTGGTTCACAGGATGAGGAAGAACCAAATGATGAGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005788 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016592 GO:0022414 GO:0030154 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044432 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044706 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051704 GO:0060560 GO:0070013 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko03060 ko04141 ko04918 ko05020 map03060 map04141 map04918 map05020

Pfam Domains

Protein Families

HSP70

Protein Analysis

494

Amino Acids

54.69

Weight (kDa)

5.01

Isoelectric Point (pI)

33.76

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09080 AT1G09080
fragaria_vesca	FvH4_6g06470
malus_domestica	MD12G1215200.v1.1
prunus_persica	Prupe.6G321700_v2.0.a1
pyrus_communis	pycom04g17760 pycom12g20120
rosa_chinensis	RchiOBHm_Chr2g0142601 RchiOBHm_Chr3g0455591 RchiOBHm_Chr3g0455611 RchiOBHm_Chr3g0455631
rosa_laevigata	RLG00000019973 RLG00000019974 RLG00000025345 RLG00000025351
rosa_multiflora	Rmu_co8491437.1_g000001 Rmu_sc0000223.1_g000003 Rmu_sc0005164.1_g000002 Rmu_sc0015502.1_g000002
rosa_roxburghii	Rroxscaffold_2G00102460
rosa_rugosa	Rorug03G0008500 Rorug03G0008700
rosa_samantha	Rh2AG426400 Rh2BG436000 Rh2CG412900 Rh2DG445800 Rh3AG067800 Rh3AG068000 Rh3DG070300
rosa_wichuraiana	Rw2G034940 Rw3G005440 Rw3G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	853
Acc16I	TGCGCA	1 cut(s)	48
AccBSI	CCGCTC	1 cut(s)	1428
AccI	GTMKAC	1 cut(s)	245
AccII	CGCG	1 cut(s)	1067
AciI	CCGC	7 cut(s)	123, 189, 735, 977, 1065, 1262, 1428
AclWI	GGATC	1 cut(s)	677
AcoI	YGGCCR	1 cut(s)	120
AcuI	CTGAAG	1 cut(s)	1394
AcyI	GRCGYC	2 cut(s)	12, 1108
AfaI	GTAC	3 cut(s)	193, 604, 911
AfiI	CCNNNNNNNGG	3 cut(s)	418, 665, 1006
AflIII	ACRYGT	1 cut(s)	1042
AgeI	ACCGGT	1 cut(s)	968
AgsI	TTSAA	7 cut(s)	40, 437, 499, 514, 562, 634, 710
AjnI	CCWGG	3 cut(s)	588, 1116, 1196
AluBI	AGCT	7 cut(s)	359, 392, 927, 949, 1258, 1372, 1477
AluI	AGCT	7 cut(s)	359, 392, 927, 949, 1258, 1372, 1477
Alw21I	GWGCWC	1 cut(s)	1238
Alw26I	GTCTC	4 cut(s)	249, 861, 1080, 1205
AlwI	GGATC	1 cut(s)	677
Ama87I	CYCGRG	1 cut(s)	1319
AoxI	GGCC	3 cut(s)	120, 527, 1062
ApeKI	GCWGC	2 cut(s)	125, 701
AsiGI	ACCGGT	1 cut(s)	968
AspLEI	GCGC	2 cut(s)	49, 1318
AspS9I	GGNCC	2 cut(s)	527, 880
AsuC2I	CCSGG	1 cut(s)	85
AsuHPI	GGTGA	8 cut(s)	250, 544, 859, 929, 958, 1021, 1150, 1414
AsuII	TTCGAA	1 cut(s)	373
AvaI	CYCGRG	1 cut(s)	1319
AvaII	GGWCC	1 cut(s)	880
AxyI	CCTNAGG	1 cut(s)	615
BaeGI	GKGCMC	1 cut(s)	999
BanII	GRGCYC	2 cut(s)	90, 117
BauI	CACGAG	1 cut(s)	578
BbsI	GAAGAC	1 cut(s)	856
Bbv12I	GWGCWC	1 cut(s)	1238
BbvCI	CCTCAGC	2 cut(s)	1053, 1149
BbvI	GCAGC	2 cut(s)	137, 688
BccI	CCATC	3 cut(s)	289, 641, 908
BceAI	ACGGC	2 cut(s)	135, 466
BcgI	CGANNNNNNTGC	2 cut(s)	404, 438
BciT130I	CCWGG	3 cut(s)	590, 1118, 1198
BclI	TGATCA	2 cut(s)	3, 315
BcnI	CCSGG	1 cut(s)	85
BcoDI	GTCTC	4 cut(s)	249, 861, 1080, 1205
BfaI	CTAG	2 cut(s)	143, 483
BfmI	CTRYAG	2 cut(s)	702, 1478
BfoI	RGCGCY	1 cut(s)	1319
BglII	AGATCT	1 cut(s)	751
BisI	GCNGC	4 cut(s)	123, 126, 702, 1065
BlsI	GCNGC	4 cut(s)	124, 127, 703, 1066
Bme1390I	CCNGG	4 cut(s)	85, 590, 1118, 1198
Bme18I	GGWCC	1 cut(s)	880
BmeT110I	CYCGRG	1 cut(s)	1319
BmgT120I	GGNCC	2 cut(s)	527, 880
BmiI	GGNNCC	2 cut(s)	468, 528
BmrFI	CCNGG	4 cut(s)	85, 590, 1118, 1198
BmsI	GCATC	8 cut(s)	34, 58, 84, 216, 424, 541, 1015, 1183
BpiI	GAAGAC	1 cut(s)	856
Bpu10I	CCTNAGC	2 cut(s)	1053, 1149
Bpu14I	TTCGAA	1 cut(s)	373
BpuEI	CTTGAG	2 cut(s)	843, 1229
BpuMI	CCSGG	1 cut(s)	85
BsaAI	YACGTR	1 cut(s)	1045
BsaHI	GRCGYC	2 cut(s)	12, 1108
BsaI	GGTCTC	1 cut(s)	1205
BsaJI	CCNNGG	7 cut(s)	15, 63, 117, 522, 827, 986, 1161
BsaWI	WCCGGW	2 cut(s)	968, 1399
BsaXI	ACNNNNNCTCC	2 cut(s)	1376, 1406
Bsc4I	CCNNNNNNNGG	3 cut(s)	418, 665, 1006
Bse118I	RCCGGY	2 cut(s)	529, 968
Bse1I	ACTGG	1 cut(s)	174
Bse21I	CCTNAGG	1 cut(s)	615
Bse3DI	GCAATG	2 cut(s)	78, 129
BseBI	CCWGG	3 cut(s)	590, 1118, 1198
BseDI	CCNNGG	7 cut(s)	15, 63, 117, 522, 827, 986, 1161
BseGI	GGATG	6 cut(s)	73, 840, 1285, 1336, 1360, 1459
BseLI	CCNNNNNNNGG	3 cut(s)	418, 665, 1006
BseMI	GCAATG	2 cut(s)	78, 129
BseMII	CTCAG	3 cut(s)	736, 1044, 1140
BseNI	ACTGG	1 cut(s)	174
BseRI	GAGGAG	4 cut(s)	285, 506, 1166, 1178
BseSI	GKGCMC	1 cut(s)	999
BseX3I	CGGCCG	1 cut(s)	120
BseXI	GCAGC	2 cut(s)	137, 688
Bsh1236I	CGCG	1 cut(s)	1067
Bsh1285I	CGRYCG	1 cut(s)	123
BshFI	GGCC	3 cut(s)	122, 529, 1064
BshTI	ACCGGT	1 cut(s)	968
BsiEI	CGRYCG	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	1238
BsiHKCI	CYCGRG	1 cut(s)	1319
BsiSI	CCGG	4 cut(s)	84, 530, 969, 1400
BslI	CCNNNNNNNGG	3 cut(s)	418, 665, 1006
BsmAI	GTCTC	4 cut(s)	249, 861, 1080, 1205
BsnI	GGCC	3 cut(s)	122, 529, 1064
Bso31I	GGTCTC	1 cut(s)	1205
BsoBI	CYCGRG	1 cut(s)	1319
Bsp119I	TTCGAA	1 cut(s)	373
Bsp1286I	GDGCHC	4 cut(s)	90, 117, 999, 1238
Bsp143I	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
Bsp19I	CCATGG	1 cut(s)	522
BspACI	CCGC	7 cut(s)	123, 189, 735, 977, 1065, 1262, 1428
BspANI	GGCC	3 cut(s)	122, 529, 1064
BspCNI	CTCAG	3 cut(s)	735, 1045, 1141
BspFNI	CGCG	1 cut(s)	1067
BspHI	TCATGA	1 cut(s)	810
BspLI	GGNNCC	2 cut(s)	468, 528
BspMAI	CTGCAG	1 cut(s)	706
BspPI	GGATC	1 cut(s)	677
BspQI	GCTCTTC	2 cut(s)	1226, 1418
BspT104I	TTCGAA	1 cut(s)	373
BspTNI	GGTCTC	1 cut(s)	1205
BsrBI	CCGCTC	1 cut(s)	1428
BsrDI	GCAATG	2 cut(s)	78, 129
BsrFI	RCCGGY	2 cut(s)	529, 968
BsrI	ACTGG	1 cut(s)	174
BssAI	RCCGGY	2 cut(s)	529, 968
BssECI	CCNNGG	7 cut(s)	15, 63, 117, 522, 827, 986, 1161
BssMI	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
BssNI	GRCGYC	2 cut(s)	12, 1108
BssSI	CACGAG	1 cut(s)	578
BssT1I	CCWWGG	4 cut(s)	63, 522, 827, 986
Bst2BI	CACGAG	1 cut(s)	578
Bst2UI	CCWGG	3 cut(s)	590, 1118, 1198
Bst4CI	ACNGT	5 cut(s)	25, 799, 838, 895, 1240
Bst6I	CTCTTC	2 cut(s)	1226, 1418
BstACI	GRCGYC	2 cut(s)	12, 1108
BstAPI	GCANNNNNTGC	2 cut(s)	131, 698
BstBAI	YACGTR	1 cut(s)	1045
BstBI	TTCGAA	1 cut(s)	373
BstC8I	GCNNGC	7 cut(s)	57, 73, 413, 556, 981, 1260, 1304
BstDEI	CTNAG	6 cut(s)	393, 615, 722, 1053, 1112, 1149
BstDSI	CCRYGG	3 cut(s)	15, 117, 522
BstEII	GGTNACC	1 cut(s)	19
BstF5I	GGATG	6 cut(s)	73, 840, 1285, 1336, 1360, 1459
BstFNI	CGCG	1 cut(s)	1067
BstH2I	RGCGCY	1 cut(s)	1319
BstHHI	GCGC	2 cut(s)	49, 1318
BstKTI	GATC	8 cut(s)	6, 181, 318, 672, 754, 964, 1092, 1296
BstMAI	GTCTC	4 cut(s)	249, 861, 1080, 1205
BstMBI	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
BstMCI	CGRYCG	1 cut(s)	123
BstMWI	GCNNNNNNNGC	5 cut(s)	131, 398, 698, 1233, 1324
BstNI	CCWGG	3 cut(s)	590, 1118, 1198
BstPI	GGTNACC	1 cut(s)	19
BstSCI	CCNGG	4 cut(s)	83, 588, 1116, 1196
BstSFI	CTRYAG	2 cut(s)	702, 1478
BstSLI	GKGCMC	1 cut(s)	999
BstUI	CGCG	1 cut(s)	1067
BstV1I	GCAGC	2 cut(s)	137, 688
BstV2I	GAAGAC	1 cut(s)	856
BstX2I	RGATCY	1 cut(s)	751
BstYI	RGATCY	1 cut(s)	751
BstZI	CGGCCG	1 cut(s)	120
Bsu36I	CCTNAGG	1 cut(s)	615
BsuRI	GGCC	3 cut(s)	122, 529, 1064
BtgI	CCRYGG	3 cut(s)	15, 117, 522
BtsCI	GGATG	6 cut(s)	73, 840, 1285, 1336, 1360, 1459
BtsI	GCAGTG	1 cut(s)	1395
BtsIMutI	CAGTG	4 cut(s)	730, 834, 1236, 1395
Cac8I	GCNNGC	7 cut(s)	57, 73, 413, 556, 981, 1260, 1304
CciI	TCATGA	1 cut(s)	810
CfoI	GCGC	2 cut(s)	49, 1318
Cfr10I	RCCGGY	2 cut(s)	529, 968
Cfr13I	GGNCC	2 cut(s)	527, 880
CseI	GACGC	2 cut(s)	20, 1097
Csp6I	GTAC	3 cut(s)	192, 603, 910
CspAI	ACCGGT	1 cut(s)	968
CviAII	CATG	3 cut(s)	523, 811, 1228
CviQI	GTAC	3 cut(s)	192, 603, 910
DdeI	CTNAG	6 cut(s)	393, 615, 722, 1053, 1112, 1149
DpnI	GATC	8 cut(s)	5, 180, 317, 671, 753, 963, 1091, 1295
DpnII	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
DrdI	GACNNNNNNGTC	1 cut(s)	853
DseDI	GACNNNNNNGTC	1 cut(s)	853
EaeI	YGGCCR	1 cut(s)	120
EagI	CGGCCG	1 cut(s)	120
Eam1104I	CTCTTC	2 cut(s)	1226, 1418
EarI	CTCTTC	2 cut(s)	1226, 1418
EciI	GGCGGA	3 cut(s)	750, 966, 1277
EclXI	CGGCCG	1 cut(s)	120
Eco130I	CCWWGG	4 cut(s)	63, 522, 827, 986
Eco24I	GRGCYC	2 cut(s)	90, 117
Eco31I	GGTCTC	1 cut(s)	1205
Eco47I	GGWCC	1 cut(s)	880
Eco52I	CGGCCG	1 cut(s)	120
Eco57I	CTGAAG	1 cut(s)	1394
Eco81I	CCTNAGG	1 cut(s)	615
Eco88I	CYCGRG	1 cut(s)	1319
Eco91I	GGTNACC	1 cut(s)	19
EcoO65I	GGTNACC	1 cut(s)	19
EcoRII	CCWGG	3 cut(s)	588, 1116, 1196
EcoT14I	CCWWGG	4 cut(s)	63, 522, 827, 986
EcoT38I	GRGCYC	2 cut(s)	90, 117
ErhI	CCWWGG	4 cut(s)	63, 522, 827, 986
FaeI	CATG	3 cut(s)	526, 814, 1231
FalI	AAGNNNNNCTT	4 cut(s)	626, 658, 741, 773
FatI	CATG	3 cut(s)	522, 810, 1227
FauNDI	CATATG	1 cut(s)	694
FbaI	TGATCA	2 cut(s)	3, 315
FblI	GTMKAC	1 cut(s)	245
Fnu4HI	GCNGC	4 cut(s)	123, 126, 702, 1065
FokI	GGATG	6 cut(s)	80, 827, 1292, 1343, 1367, 1466
FriOI	GRGCYC	2 cut(s)	90, 117
Fsp4HI	GCNGC	4 cut(s)	123, 126, 702, 1065
FspBI	CTAG	2 cut(s)	143, 483
FspI	TGCGCA	1 cut(s)	48
GlaI	GCGC	2 cut(s)	48, 1317
GluI	GCNGC	4 cut(s)	123, 126, 702, 1065
HaeII	RGCGCY	1 cut(s)	1319
HaeIII	GGCC	3 cut(s)	122, 529, 1064
HapII	CCGG	4 cut(s)	84, 530, 969, 1400
HgaI	GACGC	2 cut(s)	20, 1097
HhaI	GCGC	2 cut(s)	49, 1318
Hin1I	GRCGYC	2 cut(s)	12, 1108
Hin1II	CATG	3 cut(s)	526, 814, 1231
Hin6I	GCGC	2 cut(s)	47, 1316
HinP1I	GCGC	2 cut(s)	47, 1316
HindIII	AAGCTT	1 cut(s)	925
HinfI	GANTC	6 cut(s)	437, 486, 609, 781, 823, 1274
HpaII	CCGG	4 cut(s)	84, 530, 969, 1400
HphI	GGTGA	8 cut(s)	250, 544, 859, 929, 958, 1021, 1150, 1414
Hpy166II	GTNNAC	3 cut(s)	246, 910, 1448
Hpy188I	TCNGA	3 cut(s)	102, 466, 1279
Hpy8I	GTNNAC	3 cut(s)	246, 910, 1448
Hpy99I	CGWCG	1 cut(s)	1367
HpyAV	CCTTC	6 cut(s)	628, 725, 908, 928, 1135, 1369
HpyCH4III	ACNGT	5 cut(s)	25, 799, 838, 895, 1240
HpyCH4IV	ACGT	1 cut(s)	1044
HpyCH4V	TGCA	7 cut(s)	71, 554, 622, 692, 704, 1028, 1395
HpyF10VI	GCNNNNNNNGC	5 cut(s)	131, 398, 698, 1233, 1324
HpyF3I	CTNAG	6 cut(s)	393, 615, 722, 1053, 1112, 1149
HpySE526I	ACGT	1 cut(s)	1044
Hsp92I	GRCGYC	2 cut(s)	12, 1108
Hsp92II	CATG	3 cut(s)	526, 814, 1231
HspAI	GCGC	2 cut(s)	47, 1316
Ksp22I	TGATCA	2 cut(s)	3, 315
Kzo9I	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
LguI	GCTCTTC	2 cut(s)	1226, 1418
LmnI	GCTCC	3 cut(s)	466, 778, 988
Lsp1109I	GCAGC	2 cut(s)	137, 688
LweI	GCATC	8 cut(s)	34, 58, 84, 216, 424, 541, 1015, 1183
MaeI	CTAG	2 cut(s)	143, 483
MaeII	ACGT	1 cut(s)	1044
MaeIII	GTNAC	3 cut(s)	19, 895, 1009
MalI	GATC	8 cut(s)	5, 180, 317, 671, 753, 963, 1091, 1295
MbiI	CCGCTC	1 cut(s)	1428
MboI	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
MflI	RGATCY	1 cut(s)	751
MhlI	GDGCHC	4 cut(s)	90, 117, 999, 1238
MluCI	AATT	2 cut(s)	818, 1002
MlyI	GAGTC	1 cut(s)	790
MmeI	TCCRAC	1 cut(s)	574
MslI	CAYNNNNRTG	1 cut(s)	905
MspI	CCGG	4 cut(s)	84, 530, 969, 1400
MspR9I	CCNGG	4 cut(s)	85, 590, 1118, 1198
MvaI	CCWGG	3 cut(s)	590, 1118, 1198
MvnI	CGCG	1 cut(s)	1067
MwoI	GCNNNNNNNGC	5 cut(s)	131, 398, 698, 1233, 1324
NciI	CCSGG	1 cut(s)	85
NcoI	CCATGG	1 cut(s)	522
NdeI	CATATG	1 cut(s)	694
NdeII	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
NlaIII	CATG	3 cut(s)	526, 814, 1231
NlaIV	GGNNCC	2 cut(s)	468, 528
NmeAIII	GCCGAG	1 cut(s)	1186
NmuCI	GTSAC	1 cut(s)	1009
NsbI	TGCGCA	1 cut(s)	48
NspV	TTCGAA	1 cut(s)	373
PaeR7I	CTCGAG	1 cut(s)	1319
PagI	TCATGA	1 cut(s)	810
PciSI	GCTCTTC	2 cut(s)	1226, 1418
PfeI	GAWTC	5 cut(s)	437, 486, 609, 823, 1274
PinAI	ACCGGT	1 cut(s)	968
PkrI	GCNGC	4 cut(s)	124, 127, 703, 1066
PleI	GAGTC	1 cut(s)	789
PpsI	GAGTC	1 cut(s)	789
Ppu21I	YACGTR	1 cut(s)	1045
Psp6I	CCWGG	3 cut(s)	588, 1116, 1196
PspEI	GGTNACC	1 cut(s)	19
PspGI	CCWGG	3 cut(s)	588, 1116, 1196
PspN4I	GGNNCC	2 cut(s)	468, 528
PspPI	GGNCC	2 cut(s)	527, 880
PspXI	VCTCGAGB	1 cut(s)	1319
PstI	CTGCAG	1 cut(s)	706
PsuI	RGATCY	1 cut(s)	751
RsaI	GTAC	3 cut(s)	193, 604, 911
RsaNI	GTAC	3 cut(s)	192, 603, 910
RseI	CAYNNNNRTG	1 cut(s)	905
SapI	GCTCTTC	2 cut(s)	1226, 1418
SatI	GCNGC	4 cut(s)	123, 126, 702, 1065
Sau3AI	GATC	8 cut(s)	3, 178, 315, 669, 751, 961, 1089, 1293
Sau96I	GGNCC	2 cut(s)	527, 880
SchI	GAGTC	1 cut(s)	790
ScrFI	CCNGG	4 cut(s)	85, 590, 1118, 1198
SduI	GDGCHC	4 cut(s)	90, 117, 999, 1238
SfaNI	GCATC	8 cut(s)	34, 58, 84, 216, 424, 541, 1015, 1183
SfcI	CTRYAG	2 cut(s)	702, 1478
Sfr274I	CTCGAG	1 cut(s)	1319
SfuI	TTCGAA	1 cut(s)	373
SinI	GGWCC	1 cut(s)	880
SlaI	CTCGAG	1 cut(s)	1319
SmiMI	CAYNNNNRTG	1 cut(s)	905
SmlI	CTYRAG	3 cut(s)	858, 1208, 1319
SmoI	CTYRAG	3 cut(s)	858, 1208, 1319
Sse9I	AATT	2 cut(s)	818, 1002
SsiI	CCGC	7 cut(s)	123, 189, 735, 977, 1065, 1262, 1428
SspMI	CTAG	2 cut(s)	143, 483
StyD4I	CCNGG	4 cut(s)	83, 588, 1116, 1196
StyI	CCWWGG	4 cut(s)	63, 522, 827, 986
TaaI	ACNGT	5 cut(s)	25, 799, 838, 895, 1240
TaiI	ACGT	1 cut(s)	1047
TaqI	TCGA	9 cut(s)	373, 424, 489, 645, 792, 1023, 1296, 1320, 1362
TasI	AATT	2 cut(s)	818, 1002
TauI	GCSGC	2 cut(s)	125, 1067
TfiI	GAWTC	5 cut(s)	437, 486, 609, 823, 1274
TscAI	CASTG	4 cut(s)	730, 841, 1243, 1395
TseFI	GTSAC	1 cut(s)	1009
TseI	GCWGC	2 cut(s)	125, 701
Tsp45I	GTSAC	1 cut(s)	1009
TspDTI	ATGAA	2 cut(s)	295, 1244
TspRI	CASTG	4 cut(s)	730, 841, 1243, 1395
VpaK11BI	GGWCC	1 cut(s)	880
XbaI	TCTAGA	1 cut(s)	142
XhoI	CTCGAG	1 cut(s)	1319
XmiI	GTMKAC	1 cut(s)	245
XspI	CTAG	2 cut(s)	143, 483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.