MD13G1003100.v1.1

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002622
Physical Location & Seq
Reverse (-)
180545 .. 184151
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1003100.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGCTTCCTTGTCTGGAATGGTTCAACGACCTCTCGTTGCGGCTGCTGCCGTTGCTGTTGCCTCGGTTTCTGCTGACATCTCTGATAAATTGCCGTCTTACCGATCCAATGACGCTTGTTCTACGTCGGGTTTATCACACATCACCTCCTTGCAAGATTGGAACTCATCCTGGGTTTCACATATTTCGGTTTCTAAGCTCGCAAACTTGTCCTTTGTCTCTAGAATTCCAGTACCTGTGCCCAACATTAACTTCCCAATTCCTAACACGGGCTGCAATTTTGTTCCCAAGCAGCTATATTCATCGGTTGCATCTTCACCTCTTCTTCTTAATCTGTATCAGTCCGCAGACCTGGTTAAAGCACGAAAGCCAGCTTCGTATAACCATGATATATCATCTACCTCCCCATCTGAGGTCTCGTACAGATGGCATTTACCTGAACCGAATGCCATAGATGTTTCAGGCGGTTCTGATTGCTCATCAGCAAAGTTCAGGACTGTGGTGGTTTTGCTAGGATGGTTGGGAGCAAAGCAAAAACATCTCAAGAAGTATGCCGAGTGGTATACTTCCAGGGGATATCATGCTATTACATTTACTTTGCCCATGTCTGACATTCTCAAATACCAACCTGGCGGGAAAGTTGAAGAGCACATCGACTCGCTTGTGAGCCATTTGGCTGATTGGTTAGAAGAGGAACATGGAAAGAACATGGTGTTCCATACTTTTAGCAATACTGGATGGTTAACGTATGGGGTAATTTTGGAGCAGTTCCAGAAGCACGATCCTTCTCTAATGGGCAGGATTCGGGGCTGCGTTGTGGATTCTGCACCTGTTGCAGCACCTGACCCCCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTTCTGAAAAAGCGTAGTGTAGCAGCAAAAGGAACAATGGACTCAAATGAGTCAGGGATGGATGCTTTGGTTGGCAGCAATAATGAAGTAGTGGCACCCAAACCTGCAGTAACCGAAGCAGCATTGCTAGTAGTCTTGGAGAAGTTCTTTGGGGTGGTTTTGAAATTTCCTACAGTGAACAGGAGGCTTTCTGATGTGTTAAGCCTATTGTCAGCGCGCCAACCAAGTTGTCCGCAATTGTATATCTACAGCTCTGCAGACAGGGTGATTCCTGCAGGGTCAGTGGAATCATTCATAAAGGAGCAAAAGAGGGCGGGACATGAGGTTAGGGCTTGCAACTTTGTGTCGACGCCACACGTTGATCATTTCAGAAACGACCCAAAACTGTACACTTGTGAGCTAACCGGGTTTCTGGAGGACTGCGTGCTTCCTTGTTGCAAACAGTCGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

444

Amino Acids

48.37

Weight (kDa)

7.99

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 166 - 430 2.1e-68 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 991
AccB1I GGYRCC 1 cut(s) 973
AccI GTMKAC 2 cut(s) 563, 1226
AccII CGCG 1 cut(s) 1096
AciI CCGC 6 cut(s) 41, 345, 465, 633, 1112, 1193
AclWI GGATC 2 cut(s) 99, 776
AcsI RAATTY 2 cut(s) 225, 1043
AcyI GRCGYC 1 cut(s) 1229
AfaI GTAC 3 cut(s) 234, 422, 1268
AfiI CCNNNNNNNGG 4 cut(s) 269, 412, 855, 856
AflIII ACRYGT 1 cut(s) 1234
AgsI TTSAA 3 cut(s) 26, 644, 1042
AjnI CCWGG 5 cut(s) 170, 351, 569, 628, 849
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 5 cut(s) 199, 295, 374, 1131, 1279
AluI AGCT 5 cut(s) 199, 295, 374, 1131, 1279
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 2 cut(s) 223, 421
AlwI GGATC 2 cut(s) 99, 776
AlwNI CAGNNNCTG 2 cut(s) 236, 842
ApoI RAATTY 2 cut(s) 225, 1043
AspLEI GCGC 2 cut(s) 1096, 1098
AsuC2I CCSGG 1 cut(s) 1285
AsuHPI GGTGA 3 cut(s) 136, 309, 1156
BaeGI GKGCMC 1 cut(s) 243
BanI GGYRCC 1 cut(s) 973
Bbv12I GWGCWC 1 cut(s) 651
BccI CCATC 5 cut(s) 415, 420, 510, 732, 931
BceAI ACGGC 2 cut(s) 35, 79
BciT130I CCWGG 5 cut(s) 172, 353, 571, 630, 851
BclI TGATCA 1 cut(s) 1240
BcnI CCSGG 1 cut(s) 1285
BcoDI GTCTC 2 cut(s) 223, 421
BfaI CTAG 3 cut(s) 222, 512, 1007
BfmI CTRYAG 6 cut(s) 873, 984, 1050, 1126, 1134, 1152
BfuAI ACCTGC 1 cut(s) 991
Bme1390I CCNGG 6 cut(s) 172, 353, 571, 630, 851, 1285
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 6 cut(s) 172, 353, 571, 630, 851, 1285
BmsI GCATC 2 cut(s) 320, 931
BpmI CTGGAG 1 cut(s) 1313
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 1285
BsaHI GRCGYC 1 cut(s) 1229
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 4 cut(s) 63, 171, 570, 849
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 4 cut(s) 269, 412, 855, 856
Bse1I ACTGG 2 cut(s) 230, 739
Bse3DI GCAATG 1 cut(s) 1001
BseBI CCWGG 5 cut(s) 172, 353, 571, 630, 851
BseDI CCNNGG 4 cut(s) 63, 171, 570, 849
BseGI GGATG 5 cut(s) 167, 521, 743, 942, 946
BseLI CCNNNNNNNGG 4 cut(s) 269, 412, 855, 856
BseMI GCAATG 1 cut(s) 1001
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 230, 739
BsePI GCGCGC 1 cut(s) 1094
BseSI GKGCMC 1 cut(s) 243
BsgI GTGCAG 1 cut(s) 810
Bsh1236I CGCG 1 cut(s) 1096
BshNI GGYRCC 1 cut(s) 973
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 1 cut(s) 1284
BslFI GGGAC 1 cut(s) 1209
BslI CCNNNNNNNGG 4 cut(s) 269, 412, 855, 856
BsmAI GTCTC 2 cut(s) 223, 421
BsmFI GGGAC 1 cut(s) 1209
BsmI GAATGC 1 cut(s) 451
Bso31I GGTCTC 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 243, 651
Bsp1407I TGTACA 1 cut(s) 1266
Bsp143I GATC 3 cut(s) 104, 781, 1240
BspACI CCGC 6 cut(s) 41, 345, 465, 633, 1112, 1193
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 1096
BspLI GGNNCC 1 cut(s) 975
BspMAI CTGCAG 4 cut(s) 877, 988, 1138, 1156
BspMI ACCTGC 1 cut(s) 991
BspPI GGATC 2 cut(s) 99, 776
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 973
BspTNI GGTCTC 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 1001
BsrGI TGTACA 1 cut(s) 1266
BsrI ACTGG 2 cut(s) 230, 739
BssECI CCNNGG 4 cut(s) 63, 171, 570, 849
BssHII GCGCGC 1 cut(s) 1094
BssMI GATC 3 cut(s) 104, 781, 1240
BssNAI GTATAC 1 cut(s) 564
BssNI GRCGYC 1 cut(s) 1229
Bst1107I GTATAC 1 cut(s) 564
Bst2UI CCWGG 5 cut(s) 172, 353, 571, 630, 851
Bst4CI ACNGT 4 cut(s) 499, 1054, 1266, 1323
Bst6I CTCTTC 3 cut(s) 327, 639, 684
BstACI GRCGYC 1 cut(s) 1229
BstAPI GCANNNNNTGC 1 cut(s) 833
BstAUI TGTACA 1 cut(s) 1266
BstC8I GCNNGC 5 cut(s) 201, 372, 1096, 1213, 1304
BstDEI CTNAG 2 cut(s) 195, 411
BstF5I GGATG 5 cut(s) 167, 521, 743, 942, 946
BstFNI CGCG 1 cut(s) 1096
BstHHI GCGC 2 cut(s) 1096, 1098
BstKTI GATC 3 cut(s) 107, 784, 1243
BstMAI GTCTC 2 cut(s) 223, 421
BstMBI GATC 3 cut(s) 104, 781, 1240
BstMWI GCNNNNNNNGC 3 cut(s) 47, 53, 833
BstNI CCWGG 5 cut(s) 172, 353, 571, 630, 851
BstSCI CCNGG 6 cut(s) 170, 351, 569, 628, 849, 1283
BstSFI CTRYAG 6 cut(s) 873, 984, 1050, 1126, 1134, 1152
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 1 cut(s) 1096
BstZ17I GTATAC 1 cut(s) 564
BtsCI GGATG 5 cut(s) 167, 521, 743, 942, 946
BtsIMutI CAGTG 2 cut(s) 1059, 1167
BveI ACCTGC 1 cut(s) 991
Cac8I GCNNGC 5 cut(s) 201, 372, 1096, 1213, 1304
CaiI CAGNNNCTG 2 cut(s) 236, 842
CfoI GCGC 2 cut(s) 1096, 1098
CseI GACGC 2 cut(s) 122, 1237
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 3 cut(s) 233, 421, 1267
CviAII CATG 6 cut(s) 386, 581, 604, 698, 709, 1199
CviQI GTAC 3 cut(s) 233, 421, 1267
DdeI CTNAG 2 cut(s) 195, 411
DpnI GATC 3 cut(s) 106, 783, 1242
DpnII GATC 3 cut(s) 104, 781, 1240
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
Eam1104I CTCTTC 3 cut(s) 327, 639, 684
EarI CTCTTC 3 cut(s) 327, 639, 684
Eco31I GGTCTC 1 cut(s) 421
Eco32I GATATC 1 cut(s) 578
EcoRI GAATTC 1 cut(s) 225
EcoRII CCWGG 5 cut(s) 170, 351, 569, 628, 849
EcoRV GATATC 1 cut(s) 578
FaeI CATG 6 cut(s) 389, 584, 607, 701, 712, 1202
FaqI GGGAC 1 cut(s) 1209
FatI CATG 6 cut(s) 385, 580, 603, 697, 708, 1198
FauI CCCGC 2 cut(s) 626, 1186
FbaI TGATCA 1 cut(s) 1240
FblI GTMKAC 2 cut(s) 563, 1226
FokI GGATG 5 cut(s) 154, 528, 750, 949, 953
FspBI CTAG 3 cut(s) 222, 512, 1007
GlaI GCGC 2 cut(s) 1095, 1097
GsuI CTGGAG 1 cut(s) 1313
HapII CCGG 1 cut(s) 1284
HgaI GACGC 2 cut(s) 122, 1237
HhaI GCGC 2 cut(s) 1096, 1098
Hin1I GRCGYC 1 cut(s) 1229
Hin1II CATG 6 cut(s) 389, 584, 607, 701, 712, 1202
Hin6I GCGC 2 cut(s) 1094, 1096
HinP1I GCGC 2 cut(s) 1094, 1096
HincII GTYRAC 2 cut(s) 744, 1227
HindII GTYRAC 2 cut(s) 744, 1227
HinfI GANTC 7 cut(s) 656, 802, 821, 920, 929, 1147, 1166
HpaI GTTAAC 1 cut(s) 744
HpaII CCGG 1 cut(s) 1284
HphI GGTGA 3 cut(s) 136, 309, 1156
Hpy166II GTNNAC 5 cut(s) 564, 744, 1057, 1227, 1269
Hpy188I TCNGA 7 cut(s) 85, 412, 472, 610, 885, 1072, 1250
Hpy188III TCNNGA 6 cut(s) 15, 222, 493, 544, 772, 1292
Hpy8I GTNNAC 5 cut(s) 564, 744, 1057, 1227, 1269
Hpy99I CGWCG 2 cut(s) 130, 1231
HpyAV CCTTC 1 cut(s) 795
HpyCH4III ACNGT 4 cut(s) 499, 1054, 1266, 1323
HpyCH4IV ACGT 3 cut(s) 125, 746, 1236
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 53, 833
HpyF3I CTNAG 2 cut(s) 195, 411
HpySE526I ACGT 3 cut(s) 125, 746, 1236
Hsp92I GRCGYC 1 cut(s) 1229
Hsp92II CATG 6 cut(s) 389, 584, 607, 701, 712, 1202
HspAI GCGC 2 cut(s) 1094, 1096
Ksp22I TGATCA 1 cut(s) 1240
KspAI GTTAAC 1 cut(s) 744
Kzo9I GATC 3 cut(s) 104, 781, 1240
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 3 cut(s) 524, 763, 1180
LweI GCATC 2 cut(s) 320, 931
MabI ACCWGGT 1 cut(s) 351
MaeI CTAG 3 cut(s) 222, 512, 1007
MaeII ACGT 3 cut(s) 125, 746, 1236
MaeIII GTNAC 1 cut(s) 988
MalI GATC 3 cut(s) 106, 783, 1242
MboI GATC 3 cut(s) 104, 781, 1240
MboII GAAGA 5 cut(s) 306, 314, 317, 656, 701
MfeI CAATTG 1 cut(s) 1115
MhlI GDGCHC 2 cut(s) 243, 651
MluCI AATT 7 cut(s) 89, 225, 258, 277, 756, 1043, 1115
MlyI GAGTC 3 cut(s) 650, 914, 938
MseI TTAA 5 cut(s) 249, 330, 357, 743, 1079
MspI CCGG 1 cut(s) 1284
MspR9I CCNGG 6 cut(s) 172, 353, 571, 630, 851, 1285
MunI CAATTG 1 cut(s) 1115
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 5 cut(s) 172, 353, 571, 630, 851
MvnI CGCG 1 cut(s) 1096
MwoI GCNNNNNNNGC 3 cut(s) 47, 53, 833
NciI CCSGG 1 cut(s) 1285
NdeII GATC 3 cut(s) 104, 781, 1240
NlaIII CATG 6 cut(s) 389, 584, 607, 701, 712, 1202
NlaIV GGNNCC 1 cut(s) 975
NmeAIII GCCGAG 1 cut(s) 580
PauI GCGCGC 1 cut(s) 1094
PciSI GCTCTTC 1 cut(s) 639
PctI GAATGC 1 cut(s) 451
PfeI GAWTC 4 cut(s) 802, 821, 1147, 1166
PleI GAGTC 3 cut(s) 650, 914, 937
PpsI GAGTC 3 cut(s) 650, 914, 937
Psp6I CCWGG 5 cut(s) 170, 351, 569, 628, 849
PspGI CCWGG 5 cut(s) 170, 351, 569, 628, 849
PspN4I GGNNCC 1 cut(s) 975
PstI CTGCAG 4 cut(s) 877, 988, 1138, 1156
PstNI CAGNNNCTG 2 cut(s) 236, 842
PteI GCGCGC 1 cut(s) 1094
RsaI GTAC 3 cut(s) 234, 422, 1268
RsaNI GTAC 3 cut(s) 233, 421, 1267
SalI GTCGAC 1 cut(s) 1225
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 5 cut(s) 249, 330, 357, 743, 1079
Sau3AI GATC 3 cut(s) 104, 781, 1240
SbfI CCTGCAGG 1 cut(s) 1156
SchI GAGTC 3 cut(s) 650, 914, 938
ScrFI CCNGG 6 cut(s) 172, 353, 571, 630, 851, 1285
SdaI CCTGCAGG 1 cut(s) 1156
SduI GDGCHC 2 cut(s) 243, 651
SexAI ACCWGGT 1 cut(s) 351
SfaNI GCATC 2 cut(s) 320, 931
SfcI CTRYAG 6 cut(s) 873, 984, 1050, 1126, 1134, 1152
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse8387I CCTGCAGG 1 cut(s) 1156
Sse9I AATT 7 cut(s) 89, 225, 258, 277, 756, 1043, 1115
SsiI CCGC 6 cut(s) 41, 345, 465, 633, 1112, 1193
SspMI CTAG 3 cut(s) 222, 512, 1007
StyD4I CCNGG 6 cut(s) 170, 351, 569, 628, 849, 1283
TaaI ACNGT 4 cut(s) 499, 1054, 1266, 1323
TaiI ACGT 3 cut(s) 128, 749, 1239
TaqI TCGA 2 cut(s) 654, 1226
TasI AATT 7 cut(s) 89, 225, 258, 277, 756, 1043, 1115
TatI WGTACW 1 cut(s) 1266
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 4 cut(s) 802, 821, 1147, 1166
Tru1I TTAA 5 cut(s) 249, 330, 357, 743, 1079
Tru9I TTAA 5 cut(s) 249, 330, 357, 743, 1079
TscAI CASTG 2 cut(s) 1059, 1167
TspDTI ATGAA 3 cut(s) 291, 978, 1162
TspRI CASTG 2 cut(s) 1059, 1167
XapI RAATTY 2 cut(s) 225, 1043
XbaI TCTAGA 1 cut(s) 221
XmiI GTMKAC 2 cut(s) 563, 1226
XspI CTAG 3 cut(s) 222, 512, 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.