RchiOBHm_Chr4g0446951

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002622
Physical Location & Seq
Forward (+)
66900707 .. 66904309
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41443

Sequence Viewer

Length: 1320 bp
ATGGGTTCCTTGTCTGGAATCCTTCAACGCCCTCTAATCGCCGCCGCCGCTGTTGGCTTTGCTTCTTTTTCTTCCGACTTCTCCCTCATACTGCCCTCTCACCAATCCCAATCCCAATCCCAATCCAACCACTCCTCCTCGGATTTATCTACTTCAACGACAACTTTGTCATCGGACATTTCGCTTTCTAAGCTCGCCGCAAGCCTCTCCTTTATCTCCAGAATTCAAGTACCTGTTCCCACCATCAACTTGCCAATTCCCAATTCCCACTGCAATTCCCTCTATTCTTCGGTTGCATCTTCCCCCGCTCTTCTTAAGCTCTATCACTCTGCACACTTGGCCAAAGCGCCCAACCCAGCTGCATGTAAACATGACATAATATCATCATCCACTTCCGTCCCTGACGTCTCCTACACATGGCATTTGCCTGAGCCAAATGCTCTTGATGTTTCCCGCACCTCTGATTGTTCATCTGCAAAGATGCGGACTGTGGTCGTTTTGCTGGGATGGTTAGGCGCGAAGCAAAAGCATCTCAAGAGGTATGCCGACTGGTATACTTCCCAGGGGTTTCACGTCATCACCTTTACTTTACCCATGGCTGACATTCTCAAATACCAGCCTGGTGGTAAAGTCGAAGACCACATTGACCTGCTTGTCACTCATTTGGCTGATTGGTTAGAAGAGGAGCAGGGAAAGAACCTGCTTTTTCATACTTTCAGCAACACCGGATGGTTAACGTATGGTGTAATGTTGGAGAAGTTCCAGAAGCATGATCCTTCTGTAATGGGCAGGATTCGGGGCTGCGTTGTGGATTCCGCACCTGTTGCAGCACCTGACCCGCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTACTGAAAAAGCATAGTGTAGCAACGAAAGGAACCACAGACACTAGTGAGTTAGGGATGGATGCTTTGGTTGACAGCAAGGAAGTAGTGGCACCCAAACCTGCAGTAACTGAGGCAGCTTTGCTTTTAGTTTTGGAGAAGTTCTTTGAGGTGGTTTTGAATCTTCCTACAGTGAACAGGAGGCTTTCTGATGTTTTGGGCTTACTGTCAGCACGACAACCAAGCTGTCCGCAATTATATATCTACAGCTCTGCAGACAGAGTCATTCCTGCAGGATCTGTGGAGTCGTTCATAAAACAACAGCGGAGGGCTGGACATGAGGTTAGGGCTTGCAACTTTGTCTCGACACCTCATGTTGATCATTTCAGAAATGACCCGAAGCTGTATACTTCTGAGCTAACTCACTTTCTGGAGGACTGTGTGCTTACTTGTTGCAAACAGTCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

439

Amino Acids

47.84

Weight (kDa)

7.28

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 163 - 427 7.8e-71 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 166, 653, 842
AatII GACGTC 1 cut(s) 408
Acc36I ACCTGC 4 cut(s) 657, 708, 832, 979
AccB1I GGYRCC 1 cut(s) 961
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 2 cut(s) 554, 1256
AccII CGCG 1 cut(s) 518
AclWI GGATC 2 cut(s) 767, 1153
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 417
AflII CTTAAG 1 cut(s) 314
AgsI TTSAA 4 cut(s) 26, 156, 227, 1030
AhlI ACTAGT 1 cut(s) 914
AjiI CACGTC 1 cut(s) 574
AjnI CCWGG 2 cut(s) 561, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 247, 279
AluBI AGCT 8 cut(s) 193, 319, 359, 989, 1095, 1119, 1252, 1267
AluI AGCT 8 cut(s) 193, 319, 359, 989, 1095, 1119, 1252, 1267
Alw26I GTCTC 2 cut(s) 412, 1216
AlwI GGATC 2 cut(s) 767, 1153
AlwNI CAGNNNCTG 3 cut(s) 833, 980, 1148
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 5 cut(s) 359, 801, 827, 866, 986
ApoI RAATTY 1 cut(s) 222
AspLEI GCGC 2 cut(s) 349, 518
AsuHPI GGTGA 2 cut(s) 92, 571
BalI TGGCCA 1 cut(s) 341
BanI GGYRCC 1 cut(s) 961
BbsI GAAGAC 1 cut(s) 642
BbvI GCAGC 5 cut(s) 346, 788, 839, 878, 998
BccI CCATC 4 cut(s) 251, 501, 723, 922
BciT130I CCWGG 2 cut(s) 563, 621
BclI TGATCA 1 cut(s) 1228
BcoDI GTCTC 2 cut(s) 412, 1216
BcuI ACTAGT 1 cut(s) 914
BfaI CTAG 1 cut(s) 915
BfmI CTRYAG 6 cut(s) 864, 972, 1038, 1114, 1122, 1140
BfoI RGCGCY 1 cut(s) 350
BfrI CTTAAG 1 cut(s) 314
BfuAI ACCTGC 4 cut(s) 657, 708, 832, 979
BisI GCNGC 9 cut(s) 42, 45, 48, 198, 360, 802, 828, 867, 987
BlsI GCNGC 9 cut(s) 43, 46, 49, 199, 361, 803, 829, 868, 988
Bme1390I CCNGG 2 cut(s) 563, 621
BmgBI CACGTC 1 cut(s) 574
BmiI GGNNCC 3 cut(s) 7, 904, 963
BmrFI CCNGG 2 cut(s) 563, 621
BmsI GCATC 4 cut(s) 305, 471, 538, 922
BoxI GACNNNNGTC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 2 cut(s) 202, 1301
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 4 cut(s) 138, 561, 562, 594
BsaWI WCCGGW 1 cut(s) 725
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 563, 621
BseDI CCNNGG 4 cut(s) 138, 561, 562, 594
BseGI GGATG 5 cut(s) 386, 512, 734, 933, 937
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMII CTCAG 3 cut(s) 420, 972, 1254
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 3 cut(s) 124, 127, 698
BseXI GCAGC 5 cut(s) 346, 788, 839, 878, 998
BseYI CCCAGC 2 cut(s) 355, 502
BsgI GTGCAG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 518
BshFI GGCC 1 cut(s) 341
BshNI GGYRCC 1 cut(s) 961
BsiSI CCGG 1 cut(s) 726
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 2 cut(s) 412, 1216
BsmBI CGTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 1 cut(s) 341
Bsp143I GATC 3 cut(s) 772, 1145, 1228
Bsp19I CCATGG 1 cut(s) 594
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 3 cut(s) 421, 973, 1255
BspFNI CGCG 1 cut(s) 518
BspLI GGNNCC 3 cut(s) 7, 904, 963
BspMAI CTGCAG 4 cut(s) 868, 976, 1126, 1144
BspMI ACCTGC 4 cut(s) 657, 708, 832, 979
BspPI GGATC 2 cut(s) 767, 1153
BspQI GCTCTTC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 961
BspTI CTTAAG 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 308
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 4 cut(s) 138, 561, 562, 594
BssMI GATC 3 cut(s) 772, 1145, 1228
BssNAI GTATAC 2 cut(s) 555, 1257
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 594
Bst1107I GTATAC 2 cut(s) 555, 1257
Bst2UI CCWGG 2 cut(s) 563, 621
Bst4CI ACNGT 5 cut(s) 490, 1042, 1077, 1289, 1311
Bst6I CTCTTC 2 cut(s) 315, 675
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 824
BstC8I GCNNGC 3 cut(s) 195, 202, 1201
BstDEI CTNAG 4 cut(s) 189, 429, 981, 1263
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 5 cut(s) 386, 512, 734, 933, 937
BstFNI CGCG 1 cut(s) 518
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 2 cut(s) 349, 518
BstKTI GATC 3 cut(s) 775, 1148, 1231
BstMAI GTCTC 2 cut(s) 412, 1216
BstMBI GATC 3 cut(s) 772, 1145, 1228
BstMWI GCNNNNNNNGC 4 cut(s) 47, 190, 338, 824
BstNI CCWGG 2 cut(s) 563, 621
BstNSI RCATGY 1 cut(s) 366
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 2 cut(s) 561, 619
BstSFI CTRYAG 6 cut(s) 864, 972, 1038, 1114, 1122, 1140
BstUI CGCG 1 cut(s) 518
BstV1I GCAGC 5 cut(s) 346, 788, 839, 878, 998
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 1 cut(s) 1145
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 1 cut(s) 1145
BstZ17I GTATAC 2 cut(s) 555, 1257
BsuRI GGCC 1 cut(s) 341
BtgI CCRYGG 1 cut(s) 594
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 5 cut(s) 386, 512, 734, 933, 937
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 268, 1047
BveI ACCTGC 4 cut(s) 657, 708, 832, 979
Cac8I GCNNGC 3 cut(s) 195, 202, 1201
CaiI CAGNNNCTG 3 cut(s) 833, 980, 1148
CfoI GCGC 2 cut(s) 349, 518
Csp6I GTAC 1 cut(s) 230
CviAII CATG 7 cut(s) 363, 371, 417, 595, 770, 1187, 1223
CviQI GTAC 1 cut(s) 230
DdeI CTNAG 4 cut(s) 189, 429, 981, 1263
DpnI GATC 3 cut(s) 774, 1147, 1230
DpnII GATC 3 cut(s) 772, 1145, 1228
DrdI GACNNNNNNGTC 3 cut(s) 166, 653, 842
DseDI GACNNNNNNGTC 3 cut(s) 166, 653, 842
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 2 cut(s) 315, 675
EarI CTCTTC 2 cut(s) 315, 675
Eco130I CCWWGG 1 cut(s) 594
EcoRI GAATTC 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 561, 619
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
Esp3I CGTCTC 1 cut(s) 412
FaeI CATG 7 cut(s) 366, 374, 420, 598, 773, 1190, 1226
FaqI GGGAC 1 cut(s) 383
FatI CATG 7 cut(s) 362, 370, 416, 594, 769, 1186, 1222
FauI CCCGC 3 cut(s) 313, 461, 846
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 2 cut(s) 554, 1256
Fnu4HI GCNGC 9 cut(s) 42, 45, 48, 198, 360, 802, 828, 867, 987
FokI GGATG 5 cut(s) 373, 519, 741, 940, 944
Fsp4HI GCNGC 9 cut(s) 42, 45, 48, 198, 360, 802, 828, 867, 987
FspBI CTAG 1 cut(s) 915
GlaI GCGC 2 cut(s) 348, 517
GluI GCNGC 9 cut(s) 42, 45, 48, 198, 360, 802, 828, 867, 987
GsaI CCCAGC 2 cut(s) 359, 506
GsuI CTGGAG 2 cut(s) 202, 1301
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 1 cut(s) 341
HapII CCGG 1 cut(s) 726
HhaI GCGC 2 cut(s) 349, 518
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 7 cut(s) 366, 374, 420, 598, 773, 1190, 1226
Hin6I GCGC 2 cut(s) 347, 516
HinP1I GCGC 2 cut(s) 347, 516
HincII GTYRAC 2 cut(s) 735, 943
HindII GTYRAC 2 cut(s) 735, 943
HinfI GANTC 6 cut(s) 18, 793, 812, 1030, 1131, 1154
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 1 cut(s) 726
HphI GGTGA 2 cut(s) 92, 571
Hpy166II GTNNAC 6 cut(s) 368, 555, 735, 943, 1045, 1257
Hpy188I TCNGA 7 cut(s) 76, 142, 175, 463, 1060, 1238, 1264
Hpy188III TCNNGA 7 cut(s) 15, 219, 443, 535, 763, 1213, 1280
Hpy8I GTNNAC 6 cut(s) 368, 555, 735, 943, 1045, 1257
HpyAV CCTTC 2 cut(s) 32, 786
HpyCH4III ACNGT 5 cut(s) 490, 1042, 1077, 1289, 1311
HpyCH4IV ACGT 3 cut(s) 405, 573, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 190, 338, 824
HpyF3I CTNAG 4 cut(s) 189, 429, 981, 1263
HpySE526I ACGT 3 cut(s) 405, 573, 737
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 7 cut(s) 366, 374, 420, 598, 773, 1190, 1226
HspAI GCGC 2 cut(s) 347, 516
Ksp22I TGATCA 1 cut(s) 1228
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 3 cut(s) 772, 1145, 1228
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 5 cut(s) 346, 788, 839, 878, 998
LweI GCATC 4 cut(s) 305, 471, 538, 922
MaeI CTAG 1 cut(s) 915
MaeII ACGT 3 cut(s) 405, 573, 737
MaeIII GTNAC 2 cut(s) 655, 976
MalI GATC 3 cut(s) 774, 1147, 1230
MbiI CCGCTC 1 cut(s) 308
MboI GATC 3 cut(s) 772, 1145, 1228
MboII GAAGA 7 cut(s) 63, 279, 291, 302, 647, 692, 1025
MflI RGATCY 1 cut(s) 1145
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 5 cut(s) 222, 255, 262, 274, 1103
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 2 cut(s) 1140, 1163
MmeI TCCRAC 3 cut(s) 99, 150, 732
Mox20I TGGCCA 1 cut(s) 341
MscI TGGCCA 1 cut(s) 341
MseI TTAA 3 cut(s) 315, 734, 1318
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 3 cut(s) 50, 359, 1174
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 2 cut(s) 563, 621
MvaI CCWGG 2 cut(s) 563, 621
MvnI CGCG 1 cut(s) 518
MwoI GCNNNNNNNGC 4 cut(s) 47, 190, 338, 824
NcoI CCATGG 1 cut(s) 594
NdeII GATC 3 cut(s) 772, 1145, 1228
NlaIII CATG 7 cut(s) 366, 374, 420, 598, 773, 1190, 1226
NlaIV GGNNCC 3 cut(s) 7, 904, 963
NmuCI GTSAC 1 cut(s) 655
NspI RCATGY 1 cut(s) 366
PasI CCCWGGG 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 315
PfeI GAWTC 4 cut(s) 18, 793, 812, 1030
PflFI GACNNNGTC 1 cut(s) 1130
PkrI GCNGC 9 cut(s) 43, 46, 49, 199, 361, 803, 829, 868, 988
PleI GAGTC 2 cut(s) 1139, 1162
PpsI GAGTC 2 cut(s) 1139, 1162
PshAI GACNNNNGTC 1 cut(s) 491
Psp6I CCWGG 2 cut(s) 561, 619
PspFI CCCAGC 2 cut(s) 355, 502
PspGI CCWGG 2 cut(s) 561, 619
PspN4I GGNNCC 3 cut(s) 7, 904, 963
PstI CTGCAG 4 cut(s) 868, 976, 1126, 1144
PstNI CAGNNNCTG 3 cut(s) 833, 980, 1148
PsuI RGATCY 1 cut(s) 1145
PsyI GACNNNGTC 1 cut(s) 1130
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 3 cut(s) 315, 734, 1318
SatI GCNGC 9 cut(s) 42, 45, 48, 198, 360, 802, 828, 867, 987
Sau3AI GATC 3 cut(s) 772, 1145, 1228
SbfI CCTGCAGG 1 cut(s) 1144
SchI GAGTC 2 cut(s) 1140, 1163
ScrFI CCNGG 2 cut(s) 563, 621
SdaI CCTGCAGG 1 cut(s) 1144
SfaNI GCATC 4 cut(s) 305, 471, 538, 922
SfcI CTRYAG 6 cut(s) 864, 972, 1038, 1114, 1122, 1140
SmlI CTYRAG 2 cut(s) 314, 533
SmoI CTYRAG 2 cut(s) 314, 533
SpeI ACTAGT 1 cut(s) 914
Sse8387I CCTGCAGG 1 cut(s) 1144
Sse9I AATT 5 cut(s) 222, 255, 262, 274, 1103
SspMI CTAG 1 cut(s) 915
StyD4I CCNGG 2 cut(s) 561, 619
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 5 cut(s) 490, 1042, 1077, 1289, 1311
TaiI ACGT 3 cut(s) 408, 576, 740
TaqI TCGA 2 cut(s) 633, 1214
TasI AATT 5 cut(s) 222, 255, 262, 274, 1103
TauI GCSGC 4 cut(s) 44, 47, 50, 200
TfiI GAWTC 4 cut(s) 18, 793, 812, 1030
Tru1I TTAA 3 cut(s) 315, 734, 1318
Tru9I TTAA 3 cut(s) 315, 734, 1318
TscAI CASTG 2 cut(s) 275, 1047
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 5 cut(s) 359, 801, 827, 866, 986
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 3 cut(s) 459, 698, 1150
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 2 cut(s) 275, 1047
Tth111I GACNNNGTC 1 cut(s) 1130
Vha464I CTTAAG 1 cut(s) 314
XapI RAATTY 1 cut(s) 222
XceI RCATGY 1 cut(s) 366
XmiI GTMKAC 2 cut(s) 554, 1256
XspI CTAG 1 cut(s) 915
ZraI GACGTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.