RLG00000027396

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002622
Physical Location & Seq
Forward (+)
11211852 .. 11213171
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027396

Sequence Viewer

Length: 1320 bp
ATGAGTTCCTTGTCTGGAATCCTTCAACGACCTCTCATTGCCGCCGCCGCTGTTGGCTTTGCTTCATTTTCTTCCGACTTCTCTGTCATACTGCCTTCTCACAAATCCAACCACTCTTCTACTTCTACCTCCTCCGACTTATCAACTTCTTCCTCATCATGCGTTTCAGCCATTTCGGTTTCTAAGCTTGCAAACTTGTCCTTTGTCTCCAGAATTCGAGTACCTGTTCCCAACATTAACTTCCCAATTCCCAATTCCGACTGCAATTCTGGTCCCAAATTGCTGTATTCTTTGGTTGCATCTTCCCCTGCACTTCTCAACTTGTATCACTCTGCAGACTTGGGCAAAGCGCCGAAGCCAACTGCATATAAACATGATATATCATCATCTACTTCCGTCCCTGAGGTCTCCTACAGATGGCATTTGCCTGAGCCAAATGCATTAGATGTTTCCGGTACCTCCGATTGTTCATCAGCAAAGATAAGGACTGTGGTGGTCTTGCTGGGATGGTTAGGAGCAAAGCAAAAGCATCTTAAGAGGTATGCTGACTGGTATACTTCGCAGGGGTTTCACGTTATTACTTTTACTTTACCCATGGCTGAAATTCTCAAATACCAGCCTGGTGGTAAAGTGGAAGAGCATATTGACTTGCTTGTTACTCATTTGGCTGATTGGTTAGAAGAAGAGCAGGGAAAGAACCTGCTTTTTCATACTTTTAGCAACACTGGATGGTTAACGTATGGTGTAATGTTGGAGAATTTCCAGAAGAAGCACGATCCTTCAATAATGGGCAGGATTCGGGGATGCGTTGTGGATTCCGCACCTGTTGCAGCGCCTGACCCGCAGGTCTGGGCCTCTGGTTTCTCTGCAGCCTTTCTGAAAAAGCATAGTGTAGCAGCACAAGGGACCAGAGTCGACGCTAGTGAGTCAGGCATGGATGTTTTGATTGACAGCAAGGGAGTAGTGGCACCCAAACCTGCAGTAACTGAGGCAGCTTTGCTTTTAGTTTTGGAGAAGTTCTTTGAGGTGGTTTTGAATCTTCCTACAGTGAACAGGAGGCTTTCTGATGTTTTGGGCTTACTGTCAGCACGACAACCAAGCTGTCCGCAATTATATATCTACAGCTCGGCAGATAGAGTCATTCCTGCAGGATCTGTGGAGTCGTTCATAAAAAAGCAGCGGAGGGCTGGATATGAGGTTAGGGCTTGCAACTTTGTCTCGACACCTCATGTTGATCATTTCAGAAATGACCAGAAGCTGTATACTTCTGAGCTAACTCAGTTTCTGGAGGACTGTGTGCTTGCTTGTTGCAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

440

Amino Acids

47.8

Weight (kDa)

8.46

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 163 - 429 1.9e-69 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 83, 845
Acc36I ACCTGC 3 cut(s) 708, 835, 985
Acc65I GGTACC 1 cut(s) 455
AccB1I GGYRCC 2 cut(s) 455, 967
AccI GTMKAC 3 cut(s) 554, 915, 1262
AciI CCGC 7 cut(s) 42, 45, 48, 819, 842, 1106, 1180
AclWI GGATC 2 cut(s) 770, 1159
AcsI RAATTY 3 cut(s) 213, 603, 757
AfaI GTAC 2 cut(s) 222, 457
AfiI CCNNNNNNNGG 1 cut(s) 417
AflII CTTAAG 1 cut(s) 533
AgsI TTSAA 3 cut(s) 26, 783, 1036
AjnI CCWGG 1 cut(s) 619
AjuI GAANNNNNNNTTGG 4 cut(s) 224, 238, 256, 270
AluBI AGCT 6 cut(s) 187, 995, 1101, 1125, 1258, 1273
AluI AGCT 6 cut(s) 187, 995, 1101, 1125, 1258, 1273
Alw26I GTCTC 3 cut(s) 211, 412, 1222
AlwI GGATC 2 cut(s) 770, 1159
AlwNI CAGNNNCTG 5 cut(s) 836, 986, 1154, 1258, 1285
AoxI GGCC 1 cut(s) 852
ApeKI GCWGC 5 cut(s) 830, 869, 896, 992, 1177
ApoI RAATTY 3 cut(s) 213, 603, 757
Asp718I GGTACC 1 cut(s) 455
AspLEI GCGC 2 cut(s) 352, 835
AspS9I GGNCC 3 cut(s) 272, 852, 906
AvaII GGWCC 2 cut(s) 272, 906
AxyI CCTNAGG 1 cut(s) 402
BanI GGYRCC 2 cut(s) 455, 967
BbvI GCAGC 5 cut(s) 842, 881, 908, 1004, 1189
BccI CCATC 3 cut(s) 411, 501, 723
BciT130I CCWGG 1 cut(s) 621
BclI TGATCA 1 cut(s) 1234
BcoDI GTCTC 3 cut(s) 211, 412, 1222
BfaI CTAG 1 cut(s) 921
BfmI CTRYAG 7 cut(s) 333, 412, 867, 978, 1044, 1120, 1146
BfoI RGCGCY 2 cut(s) 353, 836
BfrI CTTAAG 1 cut(s) 533
BfuAI ACCTGC 3 cut(s) 708, 835, 985
BisI GCNGC 8 cut(s) 42, 45, 48, 831, 870, 897, 993, 1178
BlsI GCNGC 8 cut(s) 43, 46, 49, 832, 871, 898, 994, 1179
Bme1390I CCNGG 1 cut(s) 621
Bme18I GGWCC 2 cut(s) 272, 906
BmgT120I GGNCC 3 cut(s) 272, 852, 906
BmiI GGNNCC 4 cut(s) 274, 457, 907, 969
BmrFI CCNGG 1 cut(s) 621
BmsI GCATC 3 cut(s) 308, 538, 794
BoxI GACNNNNGTC 1 cut(s) 911
BpmI CTGGAG 2 cut(s) 193, 1307
Bpu10I CCTNAGC 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 594
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse1I ACTGG 2 cut(s) 554, 730
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 621
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 4 cut(s) 512, 734, 809, 943
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 5 cut(s) 393, 420, 978, 1260, 1292
BseNI ACTGG 2 cut(s) 554, 730
BseRI GAGGAG 1 cut(s) 121
BseXI GCAGC 5 cut(s) 842, 881, 908, 1004, 1189
BseYI CCCAGC 1 cut(s) 502
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 1 cut(s) 854
BshNI GGYRCC 2 cut(s) 455, 967
BsiSI CCGG 1 cut(s) 453
BslFI GGGAC 3 cut(s) 258, 383, 919
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 3 cut(s) 211, 412, 1222
BsmFI GGGAC 3 cut(s) 258, 383, 919
BsnI GGCC 1 cut(s) 854
Bso31I GGTCTC 1 cut(s) 412
Bsp143I GATC 3 cut(s) 775, 1151, 1234
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 7 cut(s) 42, 45, 48, 819, 842, 1106, 1180
BspANI GGCC 1 cut(s) 854
BspCNI CTCAG 5 cut(s) 394, 421, 979, 1261, 1291
BspLI GGNNCC 4 cut(s) 274, 457, 907, 969
BspMAI CTGCAG 4 cut(s) 337, 871, 982, 1150
BspMI ACCTGC 3 cut(s) 708, 835, 985
BspPI GGATC 2 cut(s) 770, 1159
BspQI GCTCTTC 2 cut(s) 630, 678
BspT107I GGYRCC 2 cut(s) 455, 967
BspTI CTTAAG 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 2 cut(s) 554, 730
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 3 cut(s) 775, 1151, 1234
BssNAI GTATAC 2 cut(s) 555, 1263
BssT1I CCWWGG 1 cut(s) 594
Bst1107I GTATAC 2 cut(s) 555, 1263
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 4 cut(s) 490, 1048, 1083, 1295
Bst6I CTCTTC 3 cut(s) 121, 630, 678
BstAFI CTTAAG 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 189, 1207, 1302
BstDEI CTNAG 6 cut(s) 183, 402, 429, 987, 1269, 1278
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 4 cut(s) 512, 734, 809, 943
BstH2I RGCGCY 2 cut(s) 353, 836
BstHHI GCGC 2 cut(s) 352, 835
BstKTI GATC 3 cut(s) 778, 1154, 1237
BstMAI GTCTC 3 cut(s) 211, 412, 1222
BstMBI GATC 3 cut(s) 775, 1151, 1234
BstMWI GCNNNNNNNGC 3 cut(s) 47, 827, 841
BstNI CCWGG 1 cut(s) 621
BstPAI GACNNNNGTC 1 cut(s) 911
BstSCI CCNGG 1 cut(s) 619
BstSFI CTRYAG 7 cut(s) 333, 412, 867, 978, 1044, 1120, 1146
BstV1I GCAGC 5 cut(s) 842, 881, 908, 1004, 1189
BstX2I RGATCY 1 cut(s) 1151
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 1 cut(s) 1151
BstZ17I GTATAC 2 cut(s) 555, 1263
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 1 cut(s) 854
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 4 cut(s) 512, 734, 809, 943
BtsIMutI CAGTG 2 cut(s) 723, 1053
BveI ACCTGC 3 cut(s) 708, 835, 985
Cac8I GCNNGC 3 cut(s) 189, 1207, 1302
CaiI CAGNNNCTG 5 cut(s) 836, 986, 1154, 1258, 1285
CfoI GCGC 2 cut(s) 352, 835
Cfr13I GGNCC 3 cut(s) 272, 852, 906
CseI GACGC 1 cut(s) 926
Csp6I GTAC 2 cut(s) 221, 456
CviAII CATG 5 cut(s) 159, 374, 595, 934, 1229
CviQI GTAC 2 cut(s) 221, 456
DdeI CTNAG 6 cut(s) 183, 402, 429, 987, 1269, 1278
DpnI GATC 3 cut(s) 777, 1153, 1236
DpnII GATC 3 cut(s) 775, 1151, 1234
DrdI GACNNNNNNGTC 2 cut(s) 83, 845
DseDI GACNNNNNNGTC 2 cut(s) 83, 845
Eam1104I CTCTTC 3 cut(s) 121, 630, 678
EarI CTCTTC 3 cut(s) 121, 630, 678
Eco130I CCWWGG 1 cut(s) 594
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 272, 906
Eco81I CCTNAGG 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 594
EcoT22I ATGCAT 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 5 cut(s) 162, 377, 598, 937, 1232
FaqI GGGAC 3 cut(s) 258, 383, 919
FatI CATG 5 cut(s) 158, 373, 594, 933, 1228
FauI CCCGC 1 cut(s) 849
FbaI TGATCA 1 cut(s) 1234
FblI GTMKAC 3 cut(s) 554, 915, 1262
Fnu4HI GCNGC 8 cut(s) 42, 45, 48, 831, 870, 897, 993, 1178
FokI GGATG 4 cut(s) 519, 741, 816, 950
Fsp4HI GCNGC 8 cut(s) 42, 45, 48, 831, 870, 897, 993, 1178
FspBI CTAG 1 cut(s) 921
GlaI GCGC 2 cut(s) 351, 834
GluI GCNGC 8 cut(s) 42, 45, 48, 831, 870, 897, 993, 1178
GsaI CCCAGC 1 cut(s) 506
GsuI CTGGAG 2 cut(s) 193, 1307
HaeII RGCGCY 2 cut(s) 353, 836
HaeIII GGCC 1 cut(s) 854
HapII CCGG 1 cut(s) 453
HgaI GACGC 1 cut(s) 926
HhaI GCGC 2 cut(s) 352, 835
Hin1II CATG 5 cut(s) 162, 377, 598, 937, 1232
Hin6I GCGC 2 cut(s) 350, 833
HinP1I GCGC 2 cut(s) 350, 833
HincII GTYRAC 2 cut(s) 735, 916
HindII GTYRAC 2 cut(s) 735, 916
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 8 cut(s) 18, 796, 815, 912, 926, 1036, 1137, 1160
HpaI GTTAAC 1 cut(s) 735
HpaII CCGG 1 cut(s) 453
Hpy166II GTNNAC 5 cut(s) 555, 735, 916, 1051, 1263
Hpy188I TCNGA 8 cut(s) 76, 136, 259, 463, 879, 1066, 1244, 1270
Hpy188III TCNNGA 5 cut(s) 15, 210, 763, 1219, 1286
Hpy8I GTNNAC 5 cut(s) 555, 735, 916, 1051, 1263
Hpy99I CGWCG 1 cut(s) 920
HpyAV CCTTC 3 cut(s) 32, 105, 789
HpyCH4III ACNGT 4 cut(s) 490, 1048, 1083, 1295
HpyCH4IV ACGT 2 cut(s) 573, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 827, 841
HpyF3I CTNAG 6 cut(s) 183, 402, 429, 987, 1269, 1278
HpySE526I ACGT 2 cut(s) 573, 737
Hsp92II CATG 5 cut(s) 162, 377, 598, 937, 1232
HspAI GCGC 2 cut(s) 350, 833
KpnI GGTACC 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 1234
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 3 cut(s) 775, 1151, 1234
LguI GCTCTTC 2 cut(s) 630, 678
LmnI GCTCC 1 cut(s) 515
Lsp1109I GCAGC 5 cut(s) 842, 881, 908, 1004, 1189
LweI GCATC 3 cut(s) 308, 538, 794
MaeI CTAG 1 cut(s) 921
MaeII ACGT 2 cut(s) 573, 737
MaeIII GTNAC 2 cut(s) 655, 982
MalI GATC 3 cut(s) 777, 1153, 1236
MboI GATC 3 cut(s) 775, 1151, 1234
MboII GAAGA 9 cut(s) 63, 108, 141, 294, 647, 692, 695, 778, 1031
MflI RGATCY 1 cut(s) 1151
MluCI AATT 8 cut(s) 213, 246, 253, 265, 278, 603, 757, 1109
MlyI GAGTC 4 cut(s) 921, 935, 1146, 1169
MmeI TCCRAC 5 cut(s) 99, 132, 159, 282, 732
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 3 cut(s) 237, 534, 734
MspA1I CMGCKG 2 cut(s) 50, 1180
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 621
MwoI GCNNNNNNNGC 3 cut(s) 47, 827, 841
NcoI CCATGG 1 cut(s) 594
NdeII GATC 3 cut(s) 775, 1151, 1234
NlaIII CATG 5 cut(s) 162, 377, 598, 937, 1232
NlaIV GGNNCC 4 cut(s) 274, 457, 907, 969
NmeAIII GCCGAG 1 cut(s) 1106
NsiI ATGCAT 1 cut(s) 442
PciSI GCTCTTC 2 cut(s) 630, 678
PfeI GAWTC 4 cut(s) 18, 796, 815, 1036
PkrI GCNGC 8 cut(s) 43, 46, 49, 832, 871, 898, 994, 1179
PleI GAGTC 4 cut(s) 920, 934, 1145, 1168
PpsI GAGTC 4 cut(s) 920, 934, 1145, 1168
PshAI GACNNNNGTC 1 cut(s) 911
Psp6I CCWGG 1 cut(s) 619
PspFI CCCAGC 1 cut(s) 502
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 4 cut(s) 274, 457, 907, 969
PspPI GGNCC 3 cut(s) 272, 852, 906
PstI CTGCAG 4 cut(s) 337, 871, 982, 1150
PstNI CAGNNNCTG 5 cut(s) 836, 986, 1154, 1258, 1285
PsuI RGATCY 1 cut(s) 1151
RsaI GTAC 2 cut(s) 222, 457
RsaNI GTAC 2 cut(s) 221, 456
SalI GTCGAC 1 cut(s) 914
SapI GCTCTTC 2 cut(s) 630, 678
SaqAI TTAA 3 cut(s) 237, 534, 734
SatI GCNGC 8 cut(s) 42, 45, 48, 831, 870, 897, 993, 1178
Sau3AI GATC 3 cut(s) 775, 1151, 1234
Sau96I GGNCC 3 cut(s) 272, 852, 906
SbfI CCTGCAGG 1 cut(s) 1150
SchI GAGTC 4 cut(s) 921, 935, 1146, 1169
ScrFI CCNGG 1 cut(s) 621
SdaI CCTGCAGG 1 cut(s) 1150
SfaNI GCATC 3 cut(s) 308, 538, 794
SfcI CTRYAG 7 cut(s) 333, 412, 867, 978, 1044, 1120, 1146
SinI GGWCC 2 cut(s) 272, 906
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse8387I CCTGCAGG 1 cut(s) 1150
Sse9I AATT 8 cut(s) 213, 246, 253, 265, 278, 603, 757, 1109
SsiI CCGC 7 cut(s) 42, 45, 48, 819, 842, 1106, 1180
SspMI CTAG 1 cut(s) 921
StyD4I CCNGG 1 cut(s) 619
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 4 cut(s) 490, 1048, 1083, 1295
TaiI ACGT 2 cut(s) 576, 740
TaqI TCGA 3 cut(s) 217, 915, 1220
TasI AATT 8 cut(s) 213, 246, 253, 265, 278, 603, 757, 1109
TauI GCSGC 3 cut(s) 44, 47, 50
TfiI GAWTC 4 cut(s) 18, 796, 815, 1036
Tru1I TTAA 3 cut(s) 237, 534, 734
Tru9I TTAA 3 cut(s) 237, 534, 734
TscAI CASTG 2 cut(s) 730, 1053
TseI GCWGC 5 cut(s) 830, 869, 896, 992, 1177
TspDTI ATGAA 4 cut(s) 54, 459, 698, 1156
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 2 cut(s) 730, 1053
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 2 cut(s) 272, 906
XapI RAATTY 3 cut(s) 213, 603, 757
XmiI GTMKAC 3 cut(s) 554, 915, 1262
XspI CTAG 1 cut(s) 921
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.