pycom16g00170

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002622
Physical Location & Seq
Reverse (-)
91008 .. 92188
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00170.1

Sequence Viewer

Length: 540 bp
ATGGGCAGGATTTGGGGCTGTGTTGTAGATTCTGCACCTGTTGCAGCACCTGACCCACAGGTCTGGGCTTCTGGTTTTTCTGCAGCCTTTCTGAAAAAGCGTAGTGTAGCGGCAAAAGGAACCATAGACTCAAATGAGTCAGGGATGGATGCTTTGGTTGGCAGCAATATTGGAGTAGTGGCACCCAAACCTGCAGTAACTGAAGCAGCTTTGCTAGTAGTTTTGGAGAAGTTCTTTGGGGTGGTTTTGAAGCTTCCTACAGTGAACAGGAGGCTTTCTGATGTGTTGAGCCTATTGTCAGCGCGACAACCAAGTTGTCCGCAATTGTATATCTACAGCTCTGCAGACAGGGTGATTCCTGCCGGGTCGGTGGAATCATTTATAAAGGAGCAAAGGAGGGCTGGACATGAGGTAATGGTTTGCAACTTTGTGTCGACGCCTCATGTTGATCATTTCAGAAACGACCCGAAACTGTACAGTTGTGAGCTAACCCAGTTTCTGGAGGACTGCGTGCTTACTTGTTGCAAACAGTCGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

180

Amino Acids

19.38

Weight (kDa)

7.59

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 3 - 167 3.2e-35 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AasI GACNNNNNNGTC 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 199
AccB1I GGYRCC 1 cut(s) 181
AccB7I CCANNNNNTGG 1 cut(s) 499
AccI GTMKAC 1 cut(s) 434
AccII CGCG 1 cut(s) 304
AciI CCGC 2 cut(s) 110, 320
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 437
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 4 cut(s) 209, 253, 339, 487
AluI AGCT 4 cut(s) 209, 253, 339, 487
AlwNI CAGNNNCTG 3 cut(s) 50, 200, 499
ApeKI GCWGC 4 cut(s) 44, 83, 162, 206
AspLEI GCGC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 364
BanI GGYRCC 1 cut(s) 181
BbvI GCAGC 4 cut(s) 56, 95, 174, 218
BccI CCATC 1 cut(s) 139
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 364
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 5 cut(s) 81, 192, 258, 334, 342
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 5 cut(s) 45, 84, 111, 163, 207
BlsI GCNGC 5 cut(s) 46, 85, 112, 164, 208
Bme1390I CCNGG 1 cut(s) 364
BmiI GGNNCC 2 cut(s) 121, 183
BmrFI CCNGG 1 cut(s) 364
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 487
BpmI CTGGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 437
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse1I ACTGG 1 cut(s) 493
BseGI GGATG 2 cut(s) 150, 154
BseLI CCNNNNNNNGG 1 cut(s) 499
BseNI ACTGG 1 cut(s) 493
BseXI GCAGC 4 cut(s) 56, 95, 174, 218
BsgI GTGCAG 1 cut(s) 18
Bsh1236I CGCG 1 cut(s) 304
BshNI GGYRCC 1 cut(s) 181
BsiSI CCGG 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 499
Bsp1407I TGTACA 1 cut(s) 474
Bsp143I GATC 1 cut(s) 448
BspACI CCGC 2 cut(s) 110, 320
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 2 cut(s) 121, 183
BspMAI CTGCAG 3 cut(s) 85, 196, 346
BspMI ACCTGC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 474
BsrI ACTGG 1 cut(s) 493
BssMI GATC 1 cut(s) 448
BssNI GRCGYC 1 cut(s) 437
Bst4CI ACNGT 4 cut(s) 262, 474, 479, 531
BstACI GRCGYC 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 41
BstAUI TGTACA 1 cut(s) 474
BstC8I GCNNGC 1 cut(s) 512
BstF5I GGATG 2 cut(s) 150, 154
BstFNI CGCG 1 cut(s) 304
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 1 cut(s) 451
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 362
BstSFI CTRYAG 5 cut(s) 81, 192, 258, 334, 342
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 4 cut(s) 56, 95, 174, 218
BstXI CCANNNNNNTGG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 150, 154
BtsIMutI CAGTG 1 cut(s) 267
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 512
CaiI CAGNNNCTG 3 cut(s) 50, 200, 499
CfoI GCGC 1 cut(s) 304
CseI GACGC 1 cut(s) 445
Csp6I GTAC 1 cut(s) 475
CviAII CATG 2 cut(s) 407, 443
CviQI GTAC 1 cut(s) 475
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 222
FaeI CATG 2 cut(s) 410, 446
FaiI YATR 5 cut(s) 125, 330, 383, 408, 444
FatI CATG 2 cut(s) 406, 442
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 5 cut(s) 45, 84, 111, 163, 207
FokI GGATG 2 cut(s) 157, 161
Fsp4HI GCNGC 5 cut(s) 45, 84, 111, 163, 207
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 303
GluI GCNGC 5 cut(s) 45, 84, 111, 163, 207
GsuI CTGGAG 1 cut(s) 521
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 445
HhaI GCGC 1 cut(s) 304
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 2 cut(s) 410, 446
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 5 cut(s) 29, 128, 137, 355, 374
HpaII CCGG 1 cut(s) 363
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 2 cut(s) 265, 435
Hpy188I TCNGA 3 cut(s) 93, 280, 458
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 2 cut(s) 265, 435
Hpy99I CGWCG 1 cut(s) 439
HpyCH4III ACNGT 4 cut(s) 262, 474, 479, 531
HpyCH4V TGCA 7 cut(s) 35, 44, 83, 194, 344, 423, 525
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 2 cut(s) 410, 446
HspAI GCGC 1 cut(s) 302
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 4 cut(s) 56, 95, 174, 218
LweI GCATC 1 cut(s) 139
MaeI CTAG 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 196
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MfeI CAATTG 1 cut(s) 323
MluCI AATT 1 cut(s) 323
MlyI GAGTC 2 cut(s) 122, 146
MnlI CCTC 5 cut(s) 264, 390, 403, 450, 496
MseI TTAA 1 cut(s) 538
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 364
MunI CAATTG 1 cut(s) 323
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 364
NdeII GATC 1 cut(s) 448
NlaIII CATG 2 cut(s) 410, 446
NlaIV GGNNCC 2 cut(s) 121, 183
PfeI GAWTC 3 cut(s) 29, 355, 374
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 5 cut(s) 46, 85, 112, 164, 208
PleI GAGTC 2 cut(s) 122, 145
PpsI GAGTC 2 cut(s) 122, 145
PsiI TTATAA 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 121, 183
PstI CTGCAG 3 cut(s) 85, 196, 346
PstNI CAGNNNCTG 3 cut(s) 50, 200, 499
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SalI GTCGAC 1 cut(s) 433
SaqAI TTAA 1 cut(s) 538
SatI GCNGC 5 cut(s) 45, 84, 111, 163, 207
Sau3AI GATC 1 cut(s) 448
SchI GAGTC 2 cut(s) 122, 146
ScrFI CCNGG 1 cut(s) 364
SetI ASST 9 cut(s) 40, 52, 63, 193, 211, 255, 341, 414, 489
SfaNI GCATC 1 cut(s) 139
SfcI CTRYAG 5 cut(s) 81, 192, 258, 334, 342
Sse9I AATT 1 cut(s) 323
SsiI CCGC 2 cut(s) 110, 320
SspI AATATT 1 cut(s) 169
SspMI CTAG 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 362
TaaI ACNGT 4 cut(s) 262, 474, 479, 531
TaqI TCGA 1 cut(s) 434
TasI AATT 1 cut(s) 323
TatI WGTACW 1 cut(s) 474
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 3 cut(s) 29, 355, 374
Tru1I TTAA 1 cut(s) 538
Tru9I TTAA 1 cut(s) 538
TscAI CASTG 1 cut(s) 267
TseI GCWGC 4 cut(s) 44, 83, 162, 206
TspRI CASTG 1 cut(s) 267
Van91I CCANNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 434
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.