pycom13g00260

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002622
Physical Location & Seq
Reverse (-)
167588 .. 169700
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00260.1

Sequence Viewer

Length: 1362 bp
ATGAGTATTTTGGGCGCCCTTACCAACAATATGGCTTCCTTGTCTGGAATGGTTCAACGACCTCTCGTTGCGGCTGCTGCCGTTGCTGTTGCCTCGGTTTCTGCTGACATCTCTGATAAATTGCCGTCTTACCGATCCAATGACGCTTGTTCTACGTCGGGTTTATCACACAACACCTCCTTGCAAGATTGGAACTCATCCTGGGTTTCACATATTTCGGTTTCTAAGCTCGCAAACTTGTCCTTTATCTCTAGAATTCCAGTACCTGTGCCCAACATTAACTTCCCAATTCCTAACACGGGCTGCAATTTTGTTCCCAAGCAGCTATATTCATCAGTTGCATCTTCACCTCTTCTTCTTAATCTGTATCAGTCCGCAGACCTGGTTAAAGCACGAAAGTCAGCTTCGTATAACCATGATATATCATCTACTTCCCCATCTGAGGTCTCGTACAGATGGCATTTACCTGAACCGAATGCCATAGATGTTTCAGGCGGTTCTGATTGCTCATCGGCAAAGTTCAGGACTGTGGTGGTTTTGCTAGGATGGTTGGGAGCAAAGCAAAAACATCTCAAGAAGTATGCCGAGTGGTATACTTCCAGGGGATATCATGCTATTACATTTACTTTGCCCATGTCTGACATTCTCAAATACCAACCTGGCGGGAAAGTTGAAGAGCACATCGACTCGCTTGTGAGCCATTTGGCTGATTGGTTAGAAGAGGAACATGGAAAGAACCTGGTGTTCCATACTTTTAGCAATACTGGATGGTTAACGTATGGGGTAATTTTGGAGCAGTTCCAGAAGCACGATCCTTCTCTAATGGGCAGGATTCGGGGCTGCGTTGTGGATTCTGCACCTGTTGCAGCACCTGACCCCCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTTCTGAAAAAGCGTAGTGTGGCAGCAAAAGGAACAATGGACTCAAATGAGTCAGGGATGGATGCTTTGGTTGGCAGCAATAATGGAGTAGTGGCACCCAAACCTGCAGTAACCGAAGCAGCTTTGCTAGTAGTCTTGGAGAAGTTCTTTGGGGTGGTTTTGAAATTTCCTACAGTGAACAGGAGGCTTTCTGATGTGTTGAGCCTATTGTCAGCTCGCCAACCAAGTTGTCCGCAATTGTATATCTACAGCTCTGCAGACAGGGTGATTCCTGCAGGGTCAGTGGAATCATTCATAAAGGAGCAAAAGAGGGCGGGACATGAGGTTAGGGCTTGCAACTTTGTGTCGACGCCGCACGTTGATCATTTCAGAAACGACCCAAAACTGTACACTTGTGAGCTAACCGGGTTTCTGGAGGACTGCGTGCTTCCTTGTTGCAAACAGTCGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

454

Amino Acids

49.3

Weight (kDa)

8.25

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 176 - 440 9.9e-69 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 881
Acc36I ACCTGC 1 cut(s) 1021
AccB1I GGYRCC 2 cut(s) 14, 1003
AccI GTMKAC 2 cut(s) 593, 1256
AciI CCGC 7 cut(s) 71, 375, 495, 663, 1142, 1223, 1262
AclWI GGATC 2 cut(s) 129, 806
AcsI RAATTY 2 cut(s) 255, 1073
AcyI GRCGYC 2 cut(s) 15, 1259
AfaI GTAC 3 cut(s) 264, 452, 1298
AfiI CCNNNNNNNGG 4 cut(s) 299, 442, 885, 886
AgsI TTSAA 3 cut(s) 56, 674, 1072
AjnI CCWGG 6 cut(s) 200, 381, 599, 658, 738, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AluBI AGCT 7 cut(s) 229, 325, 404, 1031, 1124, 1161, 1309
AluI AGCT 7 cut(s) 229, 325, 404, 1031, 1124, 1161, 1309
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 2 cut(s) 129, 806
AlwNI CAGNNNCTG 2 cut(s) 266, 872
ApoI RAATTY 2 cut(s) 255, 1073
AspLEI GCGC 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 1315
AsuHPI GGTGA 2 cut(s) 339, 1186
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 2 cut(s) 14, 1003
Bbv12I GWGCWC 1 cut(s) 681
BccI CCATC 5 cut(s) 445, 450, 540, 762, 961
BceAI ACGGC 2 cut(s) 65, 109
BciT130I CCWGG 6 cut(s) 202, 383, 601, 660, 740, 881
BclI TGATCA 1 cut(s) 1270
BcnI CCSGG 1 cut(s) 1315
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 3 cut(s) 252, 542, 1037
BfmI CTRYAG 6 cut(s) 903, 1014, 1080, 1156, 1164, 1182
BfoI RGCGCY 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 1021
Bme1390I CCNGG 7 cut(s) 202, 383, 601, 660, 740, 881, 1315
BmiI GGNNCC 2 cut(s) 16, 1005
BmrFI CCNGG 7 cut(s) 202, 383, 601, 660, 740, 881, 1315
BmsI GCATC 2 cut(s) 350, 961
BpmI CTGGAG 1 cut(s) 1343
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 1315
BsaHI GRCGYC 2 cut(s) 15, 1259
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 4 cut(s) 93, 201, 600, 879
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 4 cut(s) 299, 442, 885, 886
Bse1I ACTGG 2 cut(s) 260, 769
BseBI CCWGG 6 cut(s) 202, 383, 601, 660, 740, 881
BseDI CCNNGG 4 cut(s) 93, 201, 600, 879
BseGI GGATG 5 cut(s) 197, 551, 773, 972, 976
BseLI CCNNNNNNNGG 4 cut(s) 299, 442, 885, 886
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 260, 769
BseSI GKGCMC 1 cut(s) 273
BsgI GTGCAG 1 cut(s) 840
BshNI GGYRCC 2 cut(s) 14, 1003
BsiHKAI GWGCWC 1 cut(s) 681
BsiSI CCGG 1 cut(s) 1314
BslFI GGGAC 1 cut(s) 1239
BslI CCNNNNNNNGG 4 cut(s) 299, 442, 885, 886
BsmAI GTCTC 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 1239
BsmI GAATGC 1 cut(s) 481
Bso31I GGTCTC 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 273, 681
Bsp1407I TGTACA 1 cut(s) 1296
Bsp143I GATC 3 cut(s) 134, 811, 1270
BspACI CCGC 7 cut(s) 71, 375, 495, 663, 1142, 1223, 1262
BspCNI CTCAG 1 cut(s) 433
BspLI GGNNCC 2 cut(s) 16, 1005
BspMAI CTGCAG 4 cut(s) 907, 1018, 1168, 1186
BspMI ACCTGC 1 cut(s) 1021
BspPI GGATC 2 cut(s) 129, 806
BspQI GCTCTTC 1 cut(s) 669
BspT107I GGYRCC 2 cut(s) 14, 1003
BspTNI GGTCTC 1 cut(s) 451
BsrGI TGTACA 1 cut(s) 1296
BsrI ACTGG 2 cut(s) 260, 769
BssECI CCNNGG 4 cut(s) 93, 201, 600, 879
BssMI GATC 3 cut(s) 134, 811, 1270
BssNAI GTATAC 1 cut(s) 594
BssNI GRCGYC 2 cut(s) 15, 1259
Bst1107I GTATAC 1 cut(s) 594
Bst2UI CCWGG 6 cut(s) 202, 383, 601, 660, 740, 881
Bst4CI ACNGT 4 cut(s) 529, 1084, 1296, 1353
Bst6I CTCTTC 3 cut(s) 357, 669, 714
BstACI GRCGYC 2 cut(s) 15, 1259
BstAPI GCANNNNNTGC 1 cut(s) 863
BstAUI TGTACA 1 cut(s) 1296
BstC8I GCNNGC 4 cut(s) 231, 1126, 1243, 1334
BstDEI CTNAG 2 cut(s) 225, 441
BstF5I GGATG 5 cut(s) 197, 551, 773, 972, 976
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 3 cut(s) 137, 814, 1273
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 3 cut(s) 134, 811, 1270
BstMWI GCNNNNNNNGC 3 cut(s) 77, 83, 863
BstNI CCWGG 6 cut(s) 202, 383, 601, 660, 740, 881
BstSCI CCNGG 7 cut(s) 200, 381, 599, 658, 738, 879, 1313
BstSFI CTRYAG 6 cut(s) 903, 1014, 1080, 1156, 1164, 1182
BstSLI GKGCMC 1 cut(s) 273
BstXI CCANNNNNNTGG 1 cut(s) 31
BstZ17I GTATAC 1 cut(s) 594
BtsCI GGATG 5 cut(s) 197, 551, 773, 972, 976
BtsIMutI CAGTG 2 cut(s) 1089, 1197
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 4 cut(s) 231, 1126, 1243, 1334
CaiI CAGNNNCTG 2 cut(s) 266, 872
CfoI GCGC 1 cut(s) 17
CseI GACGC 2 cut(s) 152, 1267
CsiI ACCWGGT 2 cut(s) 381, 738
Csp6I GTAC 3 cut(s) 263, 451, 1297
CviAII CATG 5 cut(s) 416, 611, 634, 728, 1229
CviQI GTAC 3 cut(s) 263, 451, 1297
DdeI CTNAG 2 cut(s) 225, 441
DinI GGCGCC 1 cut(s) 16
DpnI GATC 3 cut(s) 136, 813, 1272
DpnII GATC 3 cut(s) 134, 811, 1270
DrdI GACNNNNNNGTC 1 cut(s) 881
DseDI GACNNNNNNGTC 1 cut(s) 881
Eam1104I CTCTTC 3 cut(s) 357, 669, 714
EarI CTCTTC 3 cut(s) 357, 669, 714
Eco31I GGTCTC 1 cut(s) 451
Eco32I GATATC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 6 cut(s) 200, 381, 599, 658, 738, 879
EcoRV GATATC 1 cut(s) 608
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
FaeI CATG 5 cut(s) 419, 614, 637, 731, 1232
FaqI GGGAC 1 cut(s) 1239
FatI CATG 5 cut(s) 415, 610, 633, 727, 1228
FauI CCCGC 2 cut(s) 656, 1216
FbaI TGATCA 1 cut(s) 1270
FblI GTMKAC 2 cut(s) 593, 1256
FokI GGATG 5 cut(s) 184, 558, 780, 979, 983
FspBI CTAG 3 cut(s) 252, 542, 1037
GlaI GCGC 1 cut(s) 16
GsuI CTGGAG 1 cut(s) 1343
HaeII RGCGCY 1 cut(s) 18
HapII CCGG 1 cut(s) 1314
HgaI GACGC 2 cut(s) 152, 1267
HhaI GCGC 1 cut(s) 17
Hin1I GRCGYC 2 cut(s) 15, 1259
Hin1II CATG 5 cut(s) 419, 614, 637, 731, 1232
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HincII GTYRAC 2 cut(s) 774, 1257
HindII GTYRAC 2 cut(s) 774, 1257
HinfI GANTC 7 cut(s) 686, 832, 851, 950, 959, 1177, 1196
HpaI GTTAAC 1 cut(s) 774
HpaII CCGG 1 cut(s) 1314
HphI GGTGA 2 cut(s) 339, 1186
Hpy166II GTNNAC 5 cut(s) 594, 774, 1087, 1257, 1299
Hpy188I TCNGA 7 cut(s) 115, 442, 502, 640, 915, 1102, 1280
Hpy188III TCNNGA 6 cut(s) 45, 252, 523, 574, 802, 1322
Hpy8I GTNNAC 5 cut(s) 594, 774, 1087, 1257, 1299
Hpy99I CGWCG 2 cut(s) 160, 1261
HpyAV CCTTC 1 cut(s) 825
HpyCH4III ACNGT 4 cut(s) 529, 1084, 1296, 1353
HpyCH4IV ACGT 3 cut(s) 155, 776, 1266
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 83, 863
HpyF3I CTNAG 2 cut(s) 225, 441
HpySE526I ACGT 3 cut(s) 155, 776, 1266
Hsp92I GRCGYC 2 cut(s) 15, 1259
Hsp92II CATG 5 cut(s) 419, 614, 637, 731, 1232
HspAI GCGC 1 cut(s) 15
KasI GGCGCC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 1270
KspAI GTTAAC 1 cut(s) 774
Kzo9I GATC 3 cut(s) 134, 811, 1270
LguI GCTCTTC 1 cut(s) 669
LmnI GCTCC 3 cut(s) 554, 793, 1210
LweI GCATC 2 cut(s) 350, 961
MabI ACCWGGT 2 cut(s) 381, 738
MaeI CTAG 3 cut(s) 252, 542, 1037
MaeII ACGT 3 cut(s) 155, 776, 1266
MaeIII GTNAC 1 cut(s) 1018
MalI GATC 3 cut(s) 136, 813, 1272
MboI GATC 3 cut(s) 134, 811, 1270
MboII GAAGA 5 cut(s) 336, 344, 347, 686, 731
MfeI CAATTG 1 cut(s) 1145
MhlI GDGCHC 2 cut(s) 273, 681
MluCI AATT 7 cut(s) 119, 255, 288, 307, 786, 1073, 1145
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 3 cut(s) 680, 944, 968
MseI TTAA 4 cut(s) 279, 360, 387, 773
MspI CCGG 1 cut(s) 1314
MspR9I CCNGG 7 cut(s) 202, 383, 601, 660, 740, 881, 1315
MunI CAATTG 1 cut(s) 1145
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 6 cut(s) 202, 383, 601, 660, 740, 881
MwoI GCNNNNNNNGC 3 cut(s) 77, 83, 863
NarI GGCGCC 1 cut(s) 15
NciI CCSGG 1 cut(s) 1315
NdeII GATC 3 cut(s) 134, 811, 1270
NlaIII CATG 5 cut(s) 419, 614, 637, 731, 1232
NlaIV GGNNCC 2 cut(s) 16, 1005
NmeAIII GCCGAG 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 669
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 4 cut(s) 832, 851, 1177, 1196
PleI GAGTC 3 cut(s) 680, 944, 967
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 3 cut(s) 680, 944, 967
Psp6I CCWGG 6 cut(s) 200, 381, 599, 658, 738, 879
PspGI CCWGG 6 cut(s) 200, 381, 599, 658, 738, 879
PspN4I GGNNCC 2 cut(s) 16, 1005
PstI CTGCAG 4 cut(s) 907, 1018, 1168, 1186
PstNI CAGNNNCTG 2 cut(s) 266, 872
RsaI GTAC 3 cut(s) 264, 452, 1298
RsaNI GTAC 3 cut(s) 263, 451, 1297
SalI GTCGAC 1 cut(s) 1255
SapI GCTCTTC 1 cut(s) 669
SaqAI TTAA 4 cut(s) 279, 360, 387, 773
Sau3AI GATC 3 cut(s) 134, 811, 1270
SbfI CCTGCAGG 1 cut(s) 1186
SchI GAGTC 3 cut(s) 680, 944, 968
ScrFI CCNGG 7 cut(s) 202, 383, 601, 660, 740, 881, 1315
SdaI CCTGCAGG 1 cut(s) 1186
SduI GDGCHC 2 cut(s) 273, 681
SexAI ACCWGGT 2 cut(s) 381, 738
SfaNI GCATC 2 cut(s) 350, 961
SfcI CTRYAG 6 cut(s) 903, 1014, 1080, 1156, 1164, 1182
SfoI GGCGCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse8387I CCTGCAGG 1 cut(s) 1186
Sse9I AATT 7 cut(s) 119, 255, 288, 307, 786, 1073, 1145
SsiI CCGC 7 cut(s) 71, 375, 495, 663, 1142, 1223, 1262
SspDI GGCGCC 1 cut(s) 14
SspMI CTAG 3 cut(s) 252, 542, 1037
StyD4I CCNGG 7 cut(s) 200, 381, 599, 658, 738, 879, 1313
TaaI ACNGT 4 cut(s) 529, 1084, 1296, 1353
TaiI ACGT 3 cut(s) 158, 779, 1269
TaqI TCGA 2 cut(s) 684, 1256
TasI AATT 7 cut(s) 119, 255, 288, 307, 786, 1073, 1145
TatI WGTACW 1 cut(s) 1296
TauI GCSGC 2 cut(s) 74, 1264
TfiI GAWTC 4 cut(s) 832, 851, 1177, 1196
Tru1I TTAA 4 cut(s) 279, 360, 387, 773
Tru9I TTAA 4 cut(s) 279, 360, 387, 773
TscAI CASTG 2 cut(s) 1089, 1197
TspDTI ATGAA 2 cut(s) 321, 1192
TspRI CASTG 2 cut(s) 1089, 1197
XapI RAATTY 2 cut(s) 255, 1073
XbaI TCTAGA 1 cut(s) 251
XmiI GTMKAC 2 cut(s) 593, 1256
XspI CTAG 3 cut(s) 252, 542, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.