Rroxscaffold_5G00387280

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002622
Physical Location & Seq
Forward (+)
65980170 .. 65986909
6740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387280.1

Sequence Viewer

Length: 1482 bp
ATGTTTAATTCTGATTCTCTTCCTTCCTTGTTCAACATTCATATTGAATTCACTTTTTCCCCCATTGCATTGCATTGCATTGCAGCTTTCTTGCTTTCCCAATCTCTCATTCTTCTTCCATTATTATGTTTATATTGGCATGAGAAGGAGAAGGAGTTTAAGATTTTCATCATGGGTTCCTTGTCTGGAATCCTTCAACGCCCTCTAATCGCCGCCGCCGCTGTTGGCTTTGCTTCTTTTTCTTCCGACTTCTCCCTCATACTGCCCTCTCACAAATCCAAATCCGACGACTCCTCCTCGGATTTATCAACTTCAACAACATCTTTGTCGTCACACATTTCGCTTTCTAAGCTTGCAAACCTCTCCTTCATCTCCAGAATTCAAGTACCTGTTCCCACCATTAACTTCCCAATTCCCAATTCCCACCGCAATTCCCTCTATTCTTCGGTTGCATCTTCCCCCGCTCTTCTTAAGCTCTATCGCTCTGCACACTTGGCCAGAGCGCCCAACCCAACTCCATATAAACATGATATATCATCACCATCGTCTGCTTCCGTCGTCCCTGACGTCTCCTACAGATGGCATTTGCCTGAGCCAAATGCTCTTGATGTTTCCGGCACCTCTGATTGTTCATCAGCAAAGATGAGGACTGTGGTCGTTTTGCTGGGATGGTTAGGAGCAAAGCAAAAGCATCTCAAGAGGTATGCCGACTGGTATACTTCCCAGGGGTTTCACGTCATTACCTTTACTTTACCCATGGCTGACATTCTCAAATACCAGCCTGGTGGTAAAGTCGAACACCACATTGACCTGCTTGTCACTCATTTGGCTGATTGGTTAGAAGAGGAGCAGGGAAAGAACCTGCTTTTTCATACTTTTAGCAACACCGGATGGTTAACGTATGGTGTAATGTTGGAGAAGTTCCAGAACCACGATCCTTCTGTAATGGGCAGGATTCGGGGCTGCGTTGTGGATTCCGCACCTGTTGCAGCACCTGACCCGCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTTCTGAAAAAGCATAGTGTAGCAACGAAAGGAACCACAGATGCTAGTGAGTCAGGGATGGATGCTTTGGTTGACAGCAAGGAAGTAGTGGCACCCAAACCTGCAGTAACTGAGGCAGCTTTGCTTTTAGTTTTGGAGAAGTTCTTTGAGGTGGTTTTGAATCTTCCTACAGTGAACAGGAGGCTTTCTGATGTTTTGGGCTTACTGTCAGCACGACAACCAAGCTGTCCTCAATTATATATCTACAGCTCTGCAGACAGAGTCATTCCTGCAGGATCTGTGGAGTCATTCATAAAACAACAGCGGAGGGCTGGACATGAGGTTAGGGCTTGCAACTTTGTCTCGACACCTCATGTTGATCATTTCAGAAATGACCCGAAGCTGTATACTTCTGAGCTAACTCACTTTCTGGAGGACTGCGTGCTTACTTGTTGCAAACAGTCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

493

Amino Acids

54.35

Weight (kDa)

7.68

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 217 - 481 1.3e-70 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 815, 1004
AatII GACGTC 1 cut(s) 570
Acc36I ACCTGC 4 cut(s) 819, 870, 994, 1141
AccB1I GGYRCC 2 cut(s) 617, 1123
AccBSI CCGCTC 1 cut(s) 464
AccI GTMKAC 2 cut(s) 716, 1418
AciI CCGC 8 cut(s) 213, 216, 219, 427, 462, 978, 1001, 1336
AclWI GGATC 2 cut(s) 929, 1315
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 2 cut(s) 47, 378
AcyI GRCGYC 1 cut(s) 567
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 579
AflII CTTAAG 1 cut(s) 470
AgsI TTSAA 6 cut(s) 34, 47, 197, 315, 383, 1192
AjiI CACGTC 1 cut(s) 736
AjnI CCWGG 2 cut(s) 723, 781
AjuI GAANNNNNNNTTGG 4 cut(s) 93, 125, 403, 435
AluBI AGCT 8 cut(s) 86, 352, 475, 1151, 1257, 1281, 1414, 1429
AluI AGCT 8 cut(s) 86, 352, 475, 1151, 1257, 1281, 1414, 1429
Alw26I GTCTC 2 cut(s) 574, 1378
AlwI GGATC 2 cut(s) 929, 1315
AlwNI CAGNNNCTG 3 cut(s) 995, 1142, 1310
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 5 cut(s) 83, 963, 989, 1028, 1148
ApoI RAATTY 2 cut(s) 47, 378
AspLEI GCGC 1 cut(s) 505
AsuHPI GGTGA 1 cut(s) 531
BalI TGGCCA 1 cut(s) 497
BanI GGYRCC 2 cut(s) 617, 1123
BbvI GCAGC 5 cut(s) 95, 950, 1001, 1040, 1160
BccI CCATC 5 cut(s) 550, 573, 663, 885, 1084
BciT130I CCWGG 2 cut(s) 725, 783
BclI TGATCA 1 cut(s) 1390
BcoDI GTCTC 2 cut(s) 574, 1378
BfaI CTAG 1 cut(s) 1077
BfmI CTRYAG 7 cut(s) 574, 1026, 1134, 1200, 1276, 1284, 1302
BfoI RGCGCY 1 cut(s) 506
BfrI CTTAAG 1 cut(s) 470
BfuAI ACCTGC 4 cut(s) 819, 870, 994, 1141
BisI GCNGC 8 cut(s) 84, 213, 216, 219, 964, 990, 1029, 1149
BlsI GCNGC 8 cut(s) 85, 214, 217, 220, 965, 991, 1030, 1150
Bme1390I CCNGG 2 cut(s) 725, 783
BmgBI CACGTC 1 cut(s) 736
BmiI GGNNCC 4 cut(s) 178, 619, 1066, 1125
BmrFI CCNGG 2 cut(s) 725, 783
BmsI GCATC 4 cut(s) 461, 700, 1063, 1084
BoxI GACNNNNGTC 1 cut(s) 653
BpmI CTGGAG 2 cut(s) 358, 1463
Bpu10I CCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 567
BsaJI CCNNGG 4 cut(s) 297, 723, 724, 756
BsaWI WCCGGW 1 cut(s) 887
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 278, 308
Bsc4I CCNNNNNNNGG 1 cut(s) 579
Bse1I ACTGG 1 cut(s) 716
Bse3DI GCAATG 4 cut(s) 63, 68, 73, 78
Bse8I GATNNNNATC 1 cut(s) 167
BseBI CCWGG 2 cut(s) 725, 783
BseDI CCNNGG 4 cut(s) 297, 723, 724, 756
BseGI GGATG 4 cut(s) 674, 896, 1095, 1099
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 579
BseMI GCAATG 4 cut(s) 63, 68, 73, 78
BseMII CTCAG 3 cut(s) 582, 1134, 1416
BseNI ACTGG 1 cut(s) 716
BseRI GAGGAG 3 cut(s) 283, 286, 860
BseXI GCAGC 5 cut(s) 95, 950, 1001, 1040, 1160
BseYI CCCAGC 1 cut(s) 664
BsgI GTGCAG 1 cut(s) 471
BshFI GGCC 1 cut(s) 497
BshNI GGYRCC 2 cut(s) 617, 1123
BsiSI CCGG 2 cut(s) 615, 888
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 579
BsmAI GTCTC 2 cut(s) 574, 1378
BsmBI CGTCTC 1 cut(s) 574
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 1 cut(s) 497
Bsp143I GATC 3 cut(s) 934, 1307, 1390
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 8 cut(s) 213, 216, 219, 427, 462, 978, 1001, 1336
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 3 cut(s) 583, 1135, 1417
BspLI GGNNCC 4 cut(s) 178, 619, 1066, 1125
BspMAI CTGCAG 4 cut(s) 1030, 1138, 1288, 1306
BspMI ACCTGC 4 cut(s) 819, 870, 994, 1141
BspPI GGATC 2 cut(s) 929, 1315
BspQI GCTCTTC 1 cut(s) 471
BspT107I GGYRCC 2 cut(s) 617, 1123
BspTI CTTAAG 1 cut(s) 470
BsrBI CCGCTC 1 cut(s) 464
BsrDI GCAATG 4 cut(s) 63, 68, 73, 78
BsrI ACTGG 1 cut(s) 716
BssECI CCNNGG 4 cut(s) 297, 723, 724, 756
BssMI GATC 3 cut(s) 934, 1307, 1390
BssNAI GTATAC 2 cut(s) 717, 1419
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 756
Bst1107I GTATAC 2 cut(s) 717, 1419
Bst2UI CCWGG 2 cut(s) 725, 783
Bst4CI ACNGT 4 cut(s) 652, 1204, 1239, 1473
Bst6I CTCTTC 3 cut(s) 24, 471, 837
BstACI GRCGYC 1 cut(s) 567
BstAFI CTTAAG 1 cut(s) 470
BstAPI GCANNNNNTGC 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 354, 1363, 1454
BstDEI CTNAG 4 cut(s) 348, 591, 1143, 1425
BstDSI CCRYGG 1 cut(s) 756
BstF5I GGATG 4 cut(s) 674, 896, 1095, 1099
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 3 cut(s) 937, 1310, 1393
BstMAI GTCTC 2 cut(s) 574, 1378
BstMBI GATC 3 cut(s) 934, 1307, 1390
BstMWI GCNNNNNNNGC 4 cut(s) 218, 349, 494, 986
BstNI CCWGG 2 cut(s) 725, 783
BstPAI GACNNNNGTC 1 cut(s) 653
BstSCI CCNGG 2 cut(s) 723, 781
BstSFI CTRYAG 7 cut(s) 574, 1026, 1134, 1200, 1276, 1284, 1302
BstV1I GCAGC 5 cut(s) 95, 950, 1001, 1040, 1160
BstX2I RGATCY 1 cut(s) 1307
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 1 cut(s) 1307
BstZ17I GTATAC 2 cut(s) 717, 1419
BsuRI GGCC 1 cut(s) 497
BtgI CCRYGG 1 cut(s) 756
BtrI CACGTC 1 cut(s) 736
BtsCI GGATG 4 cut(s) 674, 896, 1095, 1099
BtsIMutI CAGTG 1 cut(s) 1209
BveI ACCTGC 4 cut(s) 819, 870, 994, 1141
Cac8I GCNNGC 3 cut(s) 354, 1363, 1454
CaiI CAGNNNCTG 3 cut(s) 995, 1142, 1310
CfoI GCGC 1 cut(s) 505
Csp6I GTAC 1 cut(s) 386
CviAII CATG 6 cut(s) 140, 172, 527, 757, 1349, 1385
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 4 cut(s) 348, 591, 1143, 1425
DpnI GATC 3 cut(s) 936, 1309, 1392
DpnII GATC 3 cut(s) 934, 1307, 1390
DrdI GACNNNNNNGTC 2 cut(s) 815, 1004
DseDI GACNNNNNNGTC 2 cut(s) 815, 1004
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 3 cut(s) 24, 471, 837
EarI CTCTTC 3 cut(s) 24, 471, 837
Eco130I CCWWGG 1 cut(s) 756
EcoRI GAATTC 2 cut(s) 47, 378
EcoRII CCWGG 2 cut(s) 723, 781
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
Esp3I CGTCTC 1 cut(s) 574
FaeI CATG 6 cut(s) 143, 175, 530, 760, 1352, 1388
FaqI GGGAC 1 cut(s) 545
FatI CATG 6 cut(s) 139, 171, 526, 756, 1348, 1384
FauI CCCGC 2 cut(s) 469, 1008
FbaI TGATCA 1 cut(s) 1390
FblI GTMKAC 2 cut(s) 716, 1418
Fnu4HI GCNGC 8 cut(s) 84, 213, 216, 219, 964, 990, 1029, 1149
FokI GGATG 4 cut(s) 681, 903, 1102, 1106
Fsp4HI GCNGC 8 cut(s) 84, 213, 216, 219, 964, 990, 1029, 1149
FspBI CTAG 1 cut(s) 1077
GlaI GCGC 1 cut(s) 504
GluI GCNGC 8 cut(s) 84, 213, 216, 219, 964, 990, 1029, 1149
GsaI CCCAGC 1 cut(s) 668
GsuI CTGGAG 2 cut(s) 358, 1463
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 1 cut(s) 497
HapII CCGG 2 cut(s) 615, 888
HhaI GCGC 1 cut(s) 505
Hin1I GRCGYC 1 cut(s) 567
Hin1II CATG 6 cut(s) 143, 175, 530, 760, 1352, 1388
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HincII GTYRAC 2 cut(s) 897, 1105
HindII GTYRAC 2 cut(s) 897, 1105
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 9 cut(s) 14, 189, 290, 955, 974, 1082, 1192, 1293, 1316
HpaI GTTAAC 1 cut(s) 897
HpaII CCGG 2 cut(s) 615, 888
HphI GGTGA 1 cut(s) 531
Hpy166II GTNNAC 5 cut(s) 717, 897, 1105, 1207, 1419
Hpy188I TCNGA 9 cut(s) 13, 247, 286, 301, 625, 1038, 1222, 1400, 1426
Hpy188III TCNNGA 7 cut(s) 186, 375, 605, 697, 925, 1375, 1442
Hpy8I GTNNAC 5 cut(s) 717, 897, 1105, 1207, 1419
Hpy99I CGWCG 2 cut(s) 290, 560
HpyAV CCTTC 6 cut(s) 33, 139, 145, 203, 376, 948
HpyCH4III ACNGT 4 cut(s) 652, 1204, 1239, 1473
HpyCH4IV ACGT 3 cut(s) 567, 735, 899
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 349, 494, 986
HpyF3I CTNAG 4 cut(s) 348, 591, 1143, 1425
HpySE526I ACGT 3 cut(s) 567, 735, 899
Hsp92I GRCGYC 1 cut(s) 567
Hsp92II CATG 6 cut(s) 143, 175, 530, 760, 1352, 1388
HspAI GCGC 1 cut(s) 503
Ksp22I TGATCA 1 cut(s) 1390
KspAI GTTAAC 1 cut(s) 897
Kzo9I GATC 3 cut(s) 934, 1307, 1390
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 2 cut(s) 677, 847
Lsp1109I GCAGC 5 cut(s) 95, 950, 1001, 1040, 1160
LweI GCATC 4 cut(s) 461, 700, 1063, 1084
MaeI CTAG 1 cut(s) 1077
MaeII ACGT 3 cut(s) 567, 735, 899
MaeIII GTNAC 3 cut(s) 330, 817, 1138
MalI GATC 3 cut(s) 936, 1309, 1392
MbiI CCGCTC 1 cut(s) 464
MboI GATC 3 cut(s) 934, 1307, 1390
MboII GAAGA 9 cut(s) 11, 104, 107, 234, 435, 447, 458, 854, 1187
MflI RGATCY 1 cut(s) 1307
MlsI TGGCCA 1 cut(s) 497
MluCI AATT 7 cut(s) 7, 47, 378, 411, 418, 430, 1265
MluNI TGGCCA 1 cut(s) 497
MlyI GAGTC 4 cut(s) 284, 1091, 1302, 1325
MmeI TCCRAC 3 cut(s) 270, 309, 894
Mox20I TGGCCA 1 cut(s) 497
MscI TGGCCA 1 cut(s) 497
MseI TTAA 6 cut(s) 6, 159, 402, 471, 896, 1480
MslI CAYNNNNRTG 1 cut(s) 124
Msp20I TGGCCA 1 cut(s) 497
MspA1I CMGCKG 2 cut(s) 221, 1336
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 2 cut(s) 615, 888
MspR9I CCNGG 2 cut(s) 725, 783
MvaI CCWGG 2 cut(s) 725, 783
MwoI GCNNNNNNNGC 4 cut(s) 218, 349, 494, 986
NcoI CCATGG 1 cut(s) 756
NdeII GATC 3 cut(s) 934, 1307, 1390
NlaIII CATG 6 cut(s) 143, 175, 530, 760, 1352, 1388
NlaIV GGNNCC 4 cut(s) 178, 619, 1066, 1125
NmuCI GTSAC 2 cut(s) 330, 817
PasI CCCWGGG 1 cut(s) 724
PciSI GCTCTTC 1 cut(s) 471
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PfeI GAWTC 5 cut(s) 14, 189, 955, 974, 1192
PflFI GACNNNGTC 1 cut(s) 1292
PkrI GCNGC 8 cut(s) 85, 214, 217, 220, 965, 991, 1030, 1150
PleI GAGTC 4 cut(s) 284, 1090, 1301, 1324
PpsI GAGTC 4 cut(s) 284, 1090, 1301, 1324
PshAI GACNNNNGTC 1 cut(s) 653
Psp6I CCWGG 2 cut(s) 723, 781
PspFI CCCAGC 1 cut(s) 664
PspGI CCWGG 2 cut(s) 723, 781
PspN4I GGNNCC 4 cut(s) 178, 619, 1066, 1125
PstI CTGCAG 4 cut(s) 1030, 1138, 1288, 1306
PstNI CAGNNNCTG 3 cut(s) 995, 1142, 1310
PsuI RGATCY 1 cut(s) 1307
PsyI GACNNNGTC 1 cut(s) 1292
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
RseI CAYNNNNRTG 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 6 cut(s) 6, 159, 402, 471, 896, 1480
SatI GCNGC 8 cut(s) 84, 213, 216, 219, 964, 990, 1029, 1149
Sau3AI GATC 3 cut(s) 934, 1307, 1390
SbfI CCTGCAGG 1 cut(s) 1306
SchI GAGTC 4 cut(s) 284, 1091, 1302, 1325
ScrFI CCNGG 2 cut(s) 725, 783
SdaI CCTGCAGG 1 cut(s) 1306
SfaNI GCATC 4 cut(s) 461, 700, 1063, 1084
SfcI CTRYAG 7 cut(s) 574, 1026, 1134, 1200, 1276, 1284, 1302
SmiMI CAYNNNNRTG 1 cut(s) 124
SmlI CTYRAG 2 cut(s) 470, 695
SmoI CTYRAG 2 cut(s) 470, 695
Sse8387I CCTGCAGG 1 cut(s) 1306
Sse9I AATT 7 cut(s) 7, 47, 378, 411, 418, 430, 1265
SsiI CCGC 8 cut(s) 213, 216, 219, 427, 462, 978, 1001, 1336
SspMI CTAG 1 cut(s) 1077
StyD4I CCNGG 2 cut(s) 723, 781
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 652, 1204, 1239, 1473
TaiI ACGT 3 cut(s) 570, 738, 902
TaqI TCGA 2 cut(s) 795, 1376
TasI AATT 7 cut(s) 7, 47, 378, 411, 418, 430, 1265
TauI GCSGC 3 cut(s) 215, 218, 221
TfiI GAWTC 5 cut(s) 14, 189, 955, 974, 1192
Tru1I TTAA 6 cut(s) 6, 159, 402, 471, 896, 1480
Tru9I TTAA 6 cut(s) 6, 159, 402, 471, 896, 1480
TscAI CASTG 1 cut(s) 1209
TseFI GTSAC 2 cut(s) 330, 817
TseI GCWGC 5 cut(s) 83, 963, 989, 1028, 1148
Tsp45I GTSAC 2 cut(s) 330, 817
TspDTI ATGAA 6 cut(s) 29, 157, 358, 621, 860, 1312
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 1 cut(s) 1209
Tth111I GACNNNGTC 1 cut(s) 1292
Vha464I CTTAAG 1 cut(s) 470
XapI RAATTY 2 cut(s) 47, 378
XmiI GTMKAC 2 cut(s) 716, 1418
XspI CTAG 1 cut(s) 1077
ZraI GACGTC 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.