Rw1G006220

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002622
Physical Location & Seq
Forward (+)
12617975 .. 12626815
8841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006220.1

Sequence Viewer

Length: 1212 bp
ATGGGTTCCTTGTCTGGAATCCTTCAACGCCCTCTAATCGTCGCCGCCGCTGTTGGCTTTGCTTCTTTTTCTTCCGACTTCTCCCTCATACTGCCCTCTCACAAATCCAACTCCAACGACTCCTCCTCGGATTTATCTACTTCAACAACAACAACTTTGTCATCGGAAATTTTGCTTTCTAAACTCGCAAACCTCTCCTTTGTCTCCAGAATTCAAGTACCTATTCCCACCATTAACTTCCCAATTCCCAATTCCCACTGCAATTCCCTCTATTCATCGGTTGCATCTTCCCCCGCTCTTCTTAAGCTCTATCACTCTGCACACTTGGCCAGAGCGCCCAACCCAACTCCATATAAACATGATACATCATCATCTGCTTCTGTCCCTGACGTCTCCTACAGATGGCATTTGCCTGAGCCAAATTCTCTTGATGTTTCCGGCACCTCTGATTGTTCATCTGCAAAGATGAGGATTGTGCAAAAGCATCTCAAGAGGTATGCCGACTGGTATACTTCCCAGGGCTTTCACGTCATTACCTTTACTTTACCCATGGCTGACATTCTCAAATACCAGCCTGGTGGTAAAGTCGAACACCATATTGACCTACTTGTCAGTCATTTAGCTGATTGGTTAGAAGAGGAGCAGGGAAAGAACCTGCTTTTTCATACTTTTAGCAACACCGGATGGTTAACGTATGGTGTAATGTTGGAGAAGTTCCAGAAGCACGATCCTTCTGTAATGGGCAGGATTCGGGGTTACGTTGTGGATTCCGCACCTGTTGCAGCACCTGACCCGCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTTCTGAAAAAGCATAGTGTAGCAACGAAAGGAACCACAGACGCTAGTGAGTCAGGGATGGATGCTTTGGTTGACAACAAGGCCGTAGTGGCACCCAAACCTGCAGTAACTAAGGCATCTTTGCTTTTAGTTTTTGAGAAGTTCTTTGAGGTGGTTTTGAATCTTCCTACAACATCGGTCTGGTTTTTGTTTCTCATCCGTTTTTGGCCGATGTCTAGGGCCATAATTCTAGTAGTGGTATCAGCAATGGAAGAGGTCAAATCAATATTTTTGCCAATAATTGTGGCGAAGAAAAAAAAAAAGGGTTGCGACTTGTTTGCAACGAAGAGGCTTCAATATACATGTGGTCTCACAACTGAACGGTTCGCAAAGGAAGACTGCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

403

Amino Acids

44.34

Weight (kDa)

9.01

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 160 - 331 1.6e-29 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1197
AasI GACNNNNNNGTC 2 cut(s) 608, 797
AatII GACGTC 1 cut(s) 393
Acc36I ACCTGC 4 cut(s) 663, 787, 934, 1197
AccB1I GGYRCC 2 cut(s) 440, 916
AccBSI CCGCTC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 509
AciI CCGC 5 cut(s) 45, 48, 294, 771, 794
AclWI GGATC 1 cut(s) 722
AcoI YGGCCR 2 cut(s) 327, 1031
AcsI RAATTY 3 cut(s) 168, 210, 421
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 402
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 1166
AgsI TTSAA 5 cut(s) 26, 144, 215, 985, 1160
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 2 cut(s) 516, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
AluBI AGCT 2 cut(s) 307, 623
AluI AGCT 2 cut(s) 307, 623
Alw26I GTCTC 3 cut(s) 208, 397, 1178
AlwI GGATC 1 cut(s) 722
AlwNI CAGNNNCTG 1 cut(s) 788
AoxI GGCC 4 cut(s) 327, 906, 1031, 1044
ApeKI GCWGC 2 cut(s) 782, 821
ApoI RAATTY 3 cut(s) 168, 210, 421
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 1044
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 2 cut(s) 440, 916
BbsI GAAGAC 1 cut(s) 1206
BbvI GCAGC 2 cut(s) 794, 833
BccI CCATC 3 cut(s) 396, 678, 877
BceAI ACGGC 1 cut(s) 893
BcgI CGANNNNNNTGC 2 cut(s) 1124, 1158
BciT130I CCWGG 2 cut(s) 518, 576
BcoDI GTCTC 3 cut(s) 208, 397, 1178
BfaI CTAG 3 cut(s) 870, 1041, 1055
BfmI CTRYAG 4 cut(s) 397, 819, 927, 1204
BfoI RGCGCY 1 cut(s) 338
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 4 cut(s) 663, 787, 934, 1197
BglI GCCNNNNNGGC 1 cut(s) 914
BisI GCNGC 4 cut(s) 45, 48, 783, 822
BlsI GCNGC 4 cut(s) 46, 49, 784, 823
Bme1390I CCNGG 2 cut(s) 518, 576
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 1044
BmiI GGNNCC 4 cut(s) 7, 442, 859, 918
BmrFI CCNGG 2 cut(s) 518, 576
BmsI GCATC 4 cut(s) 293, 493, 877, 950
BpiI GAAGAC 1 cut(s) 1206
BpmI CTGGAG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 473
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 1178
BsaJI CCNNGG 4 cut(s) 126, 516, 517, 549
BsaWI WCCGGW 1 cut(s) 680
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 518, 576
BseDI CCNNGG 4 cut(s) 126, 516, 517, 549
BseGI GGATG 4 cut(s) 689, 888, 892, 1020
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 509
BseRI GAGGAG 3 cut(s) 112, 115, 653
BseXI GCAGC 2 cut(s) 794, 833
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 4 cut(s) 329, 908, 1033, 1046
BshNI GGYRCC 2 cut(s) 440, 916
BsiSI CCGG 2 cut(s) 438, 681
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 3 cut(s) 208, 397, 1178
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 4 cut(s) 329, 908, 1033, 1046
Bso31I GGTCTC 1 cut(s) 1178
Bsp143I GATC 1 cut(s) 727
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 5 cut(s) 45, 48, 294, 771, 794
BspANI GGCC 4 cut(s) 329, 908, 1033, 1046
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 4 cut(s) 7, 442, 859, 918
BspMAI CTGCAG 3 cut(s) 823, 931, 1208
BspMI ACCTGC 4 cut(s) 663, 787, 934, 1197
BspPI GGATC 1 cut(s) 722
BspQI GCTCTTC 1 cut(s) 303
BspT107I GGYRCC 2 cut(s) 440, 916
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 1178
BsrBI CCGCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 1077
BsrI ACTGG 1 cut(s) 509
BssECI CCNNGG 4 cut(s) 126, 516, 517, 549
BssMI GATC 1 cut(s) 727
BssNAI GTATAC 1 cut(s) 510
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 549
Bst1107I GTATAC 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 518, 576
Bst4CI ACNGT 1 cut(s) 1188
Bst6I CTCTTC 4 cut(s) 303, 630, 1071, 1145
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 779
BstDEI CTNAG 2 cut(s) 414, 936
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 689, 888, 892, 1020
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 730
BstMAI GTCTC 3 cut(s) 208, 397, 1178
BstMBI GATC 1 cut(s) 727
BstMWI GCNNNNNNNGC 3 cut(s) 326, 779, 914
BstNI CCWGG 2 cut(s) 518, 576
BstNSI RCATGY 1 cut(s) 1170
BstSCI CCNGG 2 cut(s) 516, 574
BstSFI CTRYAG 4 cut(s) 397, 819, 927, 1204
BstV1I GCAGC 2 cut(s) 794, 833
BstV2I GAAGAC 1 cut(s) 1206
BstXI CCANNNNNNTGG 1 cut(s) 578
BstZ17I GTATAC 1 cut(s) 510
BsuRI GGCC 4 cut(s) 329, 908, 1033, 1046
BtgI CCRYGG 1 cut(s) 549
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 4 cut(s) 689, 888, 892, 1020
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 256
BveI ACCTGC 4 cut(s) 663, 787, 934, 1197
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 1044
CseI GACGC 1 cut(s) 875
Csp6I GTAC 1 cut(s) 218
CspCI CAANNNNNGTGG 2 cut(s) 1089, 1124
CviAII CATG 3 cut(s) 359, 550, 1167
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 2 cut(s) 414, 936
DpnI GATC 1 cut(s) 729
DpnII GATC 1 cut(s) 727
DrdI GACNNNNNNGTC 2 cut(s) 608, 797
DseDI GACNNNNNNGTC 2 cut(s) 608, 797
EaeI YGGCCR 2 cut(s) 327, 1031
Eam1104I CTCTTC 4 cut(s) 303, 630, 1071, 1145
EarI CTCTTC 4 cut(s) 303, 630, 1071, 1145
Eco130I CCWWGG 1 cut(s) 549
Eco31I GGTCTC 1 cut(s) 1178
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 516, 574
EcoT14I CCWWGG 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 3 cut(s) 362, 553, 1170
FaqI GGGAC 1 cut(s) 368
FatI CATG 3 cut(s) 358, 549, 1166
FauI CCCGC 2 cut(s) 301, 801
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 4 cut(s) 45, 48, 783, 822
FokI GGATG 4 cut(s) 696, 895, 899, 1007
Fsp4HI GCNGC 4 cut(s) 45, 48, 783, 822
FspBI CTAG 3 cut(s) 870, 1041, 1055
GlaI GCGC 1 cut(s) 336
GluI GCNGC 4 cut(s) 45, 48, 783, 822
GsuI CTGGAG 1 cut(s) 190
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 4 cut(s) 329, 908, 1033, 1046
HapII CCGG 2 cut(s) 438, 681
HgaI GACGC 1 cut(s) 875
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 3 cut(s) 362, 553, 1170
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 2 cut(s) 690, 898
HindII GTYRAC 2 cut(s) 690, 898
HinfI GANTC 6 cut(s) 18, 119, 748, 767, 875, 985
HpaI GTTAAC 1 cut(s) 690
HpaII CCGG 2 cut(s) 438, 681
Hpy166II GTNNAC 3 cut(s) 510, 690, 898
Hpy188I TCNGA 5 cut(s) 76, 130, 166, 448, 831
Hpy188III TCNNGA 5 cut(s) 15, 207, 428, 490, 718
Hpy8I GTNNAC 3 cut(s) 510, 690, 898
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 2 cut(s) 32, 741
HpyCH4III ACNGT 1 cut(s) 1188
HpyCH4IV ACGT 4 cut(s) 390, 528, 692, 759
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 779, 914
HpyF3I CTNAG 2 cut(s) 414, 936
HpySE526I ACGT 4 cut(s) 390, 528, 692, 759
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 3 cut(s) 362, 553, 1170
HspAI GCGC 1 cut(s) 335
KspAI GTTAAC 1 cut(s) 690
Kzo9I GATC 1 cut(s) 727
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 640
Lsp1109I GCAGC 2 cut(s) 794, 833
LweI GCATC 4 cut(s) 293, 493, 877, 950
MaeI CTAG 3 cut(s) 870, 1041, 1055
MaeII ACGT 4 cut(s) 390, 528, 692, 759
MaeIII GTNAC 2 cut(s) 755, 931
MalI GATC 1 cut(s) 729
MbiI CCGCTC 1 cut(s) 296
MboI GATC 1 cut(s) 727
MboII GAAGA 9 cut(s) 63, 279, 290, 647, 980, 1088, 1126, 1162, 1211
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 8 cut(s) 168, 210, 243, 250, 262, 421, 1050, 1104
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 2 cut(s) 113, 884
MmeI TCCRAC 4 cut(s) 99, 132, 138, 687
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
MseI TTAA 3 cut(s) 234, 303, 689
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 2 cut(s) 438, 681
MspR9I CCNGG 2 cut(s) 518, 576
MvaI CCWGG 2 cut(s) 518, 576
MwoI GCNNNNNNNGC 3 cut(s) 326, 779, 914
NcoI CCATGG 1 cut(s) 549
NdeII GATC 1 cut(s) 727
NlaIII CATG 3 cut(s) 362, 553, 1170
NlaIV GGNNCC 4 cut(s) 7, 442, 859, 918
NspI RCATGY 1 cut(s) 1170
PaqCI CACCTGC 1 cut(s) 1197
PasI CCCWGGG 1 cut(s) 517
PciI ACATGT 1 cut(s) 1166
PciSI GCTCTTC 1 cut(s) 303
PfeI GAWTC 4 cut(s) 18, 748, 767, 985
PkrI GCNGC 4 cut(s) 46, 49, 784, 823
PleI GAGTC 2 cut(s) 113, 883
PpsI GAGTC 2 cut(s) 113, 883
PscI ACATGT 1 cut(s) 1166
Psp6I CCWGG 2 cut(s) 516, 574
PspGI CCWGG 2 cut(s) 516, 574
PspN4I GGNNCC 4 cut(s) 7, 442, 859, 918
PspPI GGNCC 1 cut(s) 1044
PstI CTGCAG 3 cut(s) 823, 931, 1208
PstNI CAGNNNCTG 1 cut(s) 788
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 3 cut(s) 234, 303, 689
SatI GCNGC 4 cut(s) 45, 48, 783, 822
Sau3AI GATC 1 cut(s) 727
Sau96I GGNCC 1 cut(s) 1044
SchI GAGTC 2 cut(s) 113, 884
ScrFI CCNGG 2 cut(s) 518, 576
SfaNI GCATC 4 cut(s) 293, 493, 877, 950
SfcI CTRYAG 4 cut(s) 397, 819, 927, 1204
SmlI CTYRAG 2 cut(s) 302, 488
SmoI CTYRAG 2 cut(s) 302, 488
Sse9I AATT 8 cut(s) 168, 210, 243, 250, 262, 421, 1050, 1104
SsiI CCGC 5 cut(s) 45, 48, 294, 771, 794
SspI AATATT 1 cut(s) 1092
SspMI CTAG 3 cut(s) 870, 1041, 1055
StyD4I CCNGG 2 cut(s) 516, 574
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 1 cut(s) 1188
TaiI ACGT 4 cut(s) 393, 531, 695, 762
TaqI TCGA 1 cut(s) 588
TaqII GACCGA 1 cut(s) 991
TasI AATT 8 cut(s) 168, 210, 243, 250, 262, 421, 1050, 1104
TauI GCSGC 2 cut(s) 47, 50
TfiI GAWTC 4 cut(s) 18, 748, 767, 985
Tru1I TTAA 3 cut(s) 234, 303, 689
Tru9I TTAA 3 cut(s) 234, 303, 689
TscAI CASTG 1 cut(s) 263
TseI GCWGC 2 cut(s) 782, 821
TspDTI ATGAA 3 cut(s) 264, 444, 653
TspGWI ACGGA 1 cut(s) 1013
TspRI CASTG 1 cut(s) 263
Vha464I CTTAAG 1 cut(s) 302
XapI RAATTY 3 cut(s) 168, 210, 421
XceI RCATGY 1 cut(s) 1170
XmiI GTMKAC 1 cut(s) 509
XspI CTAG 3 cut(s) 870, 1041, 1055
ZraI GACGTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.