Rh4AG437900

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002622
Physical Location & Seq
Forward (+)
73023568 .. 73026009
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG437900.1

Sequence Viewer

Length: 1314 bp
ATGGGTTCCTTGTCTGGAATCCTTCAACGCCCTCTAATCGCCGCCGCCGCTGTTGGCTTTGCTTCTTTTTCTTCCGACTTCTCCCTCATACTGCCCTCTCACCAATCCCAATCCCAATCCAACCACTCCTCCTCGGATTTATCTACTTCAACAACAACTTTGTCATCGGACATTTCGCTTTCTAAGCTCGCCGCAAGCCTCTCCTTTATCTCCAGAATTCAAGTACCTGTTCCCACCATCAACTTGCCAATTCCCAATTCCCACTGCAATTCCCTCTATTCTTCGGTTGCATCTTCCCCCGCTCTTCTTAAGCTCTATCACTCTGCACACTTGGCCAAGGCGCCCAACCCAGCTGCATGTAAACATGACATAATATCATCATCCACTTCCGTCCCTGACGTCTCCTACACATGGCATTTGCCTGAGCCAAATGCTCTTGATGTTTCCCGCTCCTCTGATTGTTCATTTGCAAAGATGAGGACTGTGGTCGTTTTGCTGGGATGGTTAGGAGCAAAGCAAAAGCATCTCAAGAGGTATGCCGACTGGTATACTTCCCAGGGGTTTCACGTCATTACCTTTACTTTACCCATGGCTGACATTCTCAAATACCAGCCTGGTGGTAAAGTCGAAGACCACATTGACCTGCTTGTCACTCATTTGGCTGATTGGTTAGAAGAGGAGCAGGGAAAGAACCTGCTTTTTCATACTTTCAGCAACACCGGATGGTTAACGTATGGTGTAATGTTGGAGAAGTTCCAGAAGCATGATCCTTCTGTAATGGGCAGGATTCGGGGCTGCGTTGTGGATTCCGCACCTGTTGCAGCACCTGACCCGCAGGTCTGGGCTTCTGGTTTCTCTGCAGCCTTACTGAAAAAGCATAGTGTAGCAACGAAAGGAACCACAGACACTAGTGAGTTAGGGATGGATGCTTTGGTTGACAGCAAGGAAGTAGTGGCACCCAAACCTGCAGTAACTGAGGCAGCTTTGCTTTTAGTTTTGGAGAAGTTCTTTGAGGTGGTTTTGAATCTTCCTACAGTGAACAGGAGGCTTTCTGATGTTTTGGGCTTACTGTCAGCACGACAACCAAGCTGTCCGCAATTATATATCTACAGCTCTGCAGACAGAGTCATTCCTGCAGGATCTGTGGAGTCGTTCATAAAACAACAGCGGAGGGCTGGACATGAGGTTAGGGCTTGCAACTTTGTCTCGACACCTCATGTTGATCATTTCAGAAATGACCCGAAGCTGTATACTTCTGAGCTAACTCACTTTCTGGAGGACTGTGTGCTTACTTGTTGCAAACAGTCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

437

Amino Acids

47.67

Weight (kDa)

7.28

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 161 - 425 7.7e-71 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 647, 836
AatII GACGTC 1 cut(s) 402
Acc36I ACCTGC 4 cut(s) 651, 702, 826, 973
AccB1I GGYRCC 2 cut(s) 340, 955
AccBSI CCGCTC 2 cut(s) 302, 450
AccI GTMKAC 2 cut(s) 548, 1250
AclWI GGATC 2 cut(s) 761, 1147
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 1 cut(s) 216
AcyI GRCGYC 2 cut(s) 341, 399
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 411
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 4 cut(s) 26, 150, 221, 1024
AhlI ACTAGT 1 cut(s) 908
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 2 cut(s) 555, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 241, 273
AluBI AGCT 8 cut(s) 187, 313, 353, 983, 1089, 1113, 1246, 1261
AluI AGCT 8 cut(s) 187, 313, 353, 983, 1089, 1113, 1246, 1261
Alw26I GTCTC 2 cut(s) 406, 1210
AlwI GGATC 2 cut(s) 761, 1147
AlwNI CAGNNNCTG 3 cut(s) 827, 974, 1142
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 5 cut(s) 353, 795, 821, 860, 980
ApoI RAATTY 1 cut(s) 216
AspLEI GCGC 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 92
BalI TGGCCA 1 cut(s) 335
BanI GGYRCC 2 cut(s) 340, 955
BbsI GAAGAC 1 cut(s) 636
BbvI GCAGC 5 cut(s) 340, 782, 833, 872, 992
BccI CCATC 4 cut(s) 245, 495, 717, 916
BciT130I CCWGG 2 cut(s) 557, 615
BclI TGATCA 1 cut(s) 1222
BcoDI GTCTC 2 cut(s) 406, 1210
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 1 cut(s) 909
BfmI CTRYAG 6 cut(s) 858, 966, 1032, 1108, 1116, 1134
BfoI RGCGCY 1 cut(s) 344
BfrI CTTAAG 1 cut(s) 308
BfuAI ACCTGC 4 cut(s) 651, 702, 826, 973
BisI GCNGC 9 cut(s) 42, 45, 48, 192, 354, 796, 822, 861, 981
BlsI GCNGC 9 cut(s) 43, 46, 49, 193, 355, 797, 823, 862, 982
Bme1390I CCNGG 2 cut(s) 557, 615
BmgBI CACGTC 1 cut(s) 568
BmiI GGNNCC 4 cut(s) 7, 342, 898, 957
BmrFI CCNGG 2 cut(s) 557, 615
BmsI GCATC 3 cut(s) 299, 532, 916
BoxI GACNNNNGTC 1 cut(s) 485
BpiI GAAGAC 1 cut(s) 636
BpmI CTGGAG 2 cut(s) 196, 1295
Bpu10I CCTNAGC 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 512
BsaHI GRCGYC 2 cut(s) 341, 399
BsaJI CCNNGG 5 cut(s) 132, 336, 555, 556, 588
BsaWI WCCGGW 1 cut(s) 719
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse1I ACTGG 1 cut(s) 548
BseBI CCWGG 2 cut(s) 557, 615
BseDI CCNNGG 5 cut(s) 132, 336, 555, 556, 588
BseGI GGATG 5 cut(s) 380, 506, 728, 927, 931
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 414, 966, 1248
BseNI ACTGG 1 cut(s) 548
BseRI GAGGAG 4 cut(s) 118, 121, 442, 692
BseXI GCAGC 5 cut(s) 340, 782, 833, 872, 992
BseYI CCCAGC 2 cut(s) 349, 496
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 1 cut(s) 335
BshNI GGYRCC 2 cut(s) 340, 955
BsiSI CCGG 1 cut(s) 720
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 2 cut(s) 406, 1210
BsmBI CGTCTC 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 335
Bsp143I GATC 3 cut(s) 766, 1139, 1222
Bsp19I CCATGG 1 cut(s) 588
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 3 cut(s) 415, 967, 1249
BspLI GGNNCC 4 cut(s) 7, 342, 898, 957
BspMAI CTGCAG 4 cut(s) 862, 970, 1120, 1138
BspMI ACCTGC 4 cut(s) 651, 702, 826, 973
BspPI GGATC 2 cut(s) 761, 1147
BspQI GCTCTTC 1 cut(s) 309
BspT107I GGYRCC 2 cut(s) 340, 955
BspTI CTTAAG 1 cut(s) 308
BsrBI CCGCTC 2 cut(s) 302, 450
BsrI ACTGG 1 cut(s) 548
BssECI CCNNGG 5 cut(s) 132, 336, 555, 556, 588
BssMI GATC 3 cut(s) 766, 1139, 1222
BssNAI GTATAC 2 cut(s) 549, 1251
BssNI GRCGYC 2 cut(s) 341, 399
BssT1I CCWWGG 2 cut(s) 336, 588
Bst1107I GTATAC 2 cut(s) 549, 1251
Bst2UI CCWGG 2 cut(s) 557, 615
Bst4CI ACNGT 5 cut(s) 484, 1036, 1071, 1283, 1305
Bst6I CTCTTC 2 cut(s) 309, 669
BstACI GRCGYC 2 cut(s) 341, 399
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 3 cut(s) 189, 196, 1195
BstDEI CTNAG 4 cut(s) 183, 423, 975, 1257
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 5 cut(s) 380, 506, 728, 927, 931
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 769, 1142, 1225
BstMAI GTCTC 2 cut(s) 406, 1210
BstMBI GATC 3 cut(s) 766, 1139, 1222
BstMWI GCNNNNNNNGC 4 cut(s) 47, 184, 332, 818
BstNI CCWGG 2 cut(s) 557, 615
BstNSI RCATGY 1 cut(s) 360
BstPAI GACNNNNGTC 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 555, 613
BstSFI CTRYAG 6 cut(s) 858, 966, 1032, 1108, 1116, 1134
BstV1I GCAGC 5 cut(s) 340, 782, 833, 872, 992
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 1139
BstXI CCANNNNNNTGG 1 cut(s) 617
BstYI RGATCY 1 cut(s) 1139
BstZ17I GTATAC 2 cut(s) 549, 1251
BsuRI GGCC 1 cut(s) 335
BtgI CCRYGG 1 cut(s) 588
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 5 cut(s) 380, 506, 728, 927, 931
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 2 cut(s) 262, 1041
BveI ACCTGC 4 cut(s) 651, 702, 826, 973
Cac8I GCNNGC 3 cut(s) 189, 196, 1195
CaiI CAGNNNCTG 3 cut(s) 827, 974, 1142
CfoI GCGC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 224
CviAII CATG 7 cut(s) 357, 365, 411, 589, 764, 1181, 1217
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 4 cut(s) 183, 423, 975, 1257
DinI GGCGCC 1 cut(s) 342
DpnI GATC 3 cut(s) 768, 1141, 1224
DpnII GATC 3 cut(s) 766, 1139, 1222
DrdI GACNNNNNNGTC 2 cut(s) 647, 836
DseDI GACNNNNNNGTC 2 cut(s) 647, 836
EaeI YGGCCR 1 cut(s) 333
Eam1104I CTCTTC 2 cut(s) 309, 669
EarI CTCTTC 2 cut(s) 309, 669
Eco130I CCWWGG 2 cut(s) 336, 588
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 2 cut(s) 555, 613
EcoT14I CCWWGG 2 cut(s) 336, 588
EgeI GGCGCC 1 cut(s) 342
EheI GGCGCC 1 cut(s) 342
ErhI CCWWGG 2 cut(s) 336, 588
Esp3I CGTCTC 1 cut(s) 406
FaeI CATG 7 cut(s) 360, 368, 414, 592, 767, 1184, 1220
FaqI GGGAC 1 cut(s) 377
FatI CATG 7 cut(s) 356, 364, 410, 588, 763, 1180, 1216
FauI CCCGC 3 cut(s) 307, 455, 840
FbaI TGATCA 1 cut(s) 1222
FblI GTMKAC 2 cut(s) 548, 1250
Fnu4HI GCNGC 9 cut(s) 42, 45, 48, 192, 354, 796, 822, 861, 981
FokI GGATG 5 cut(s) 367, 513, 735, 934, 938
Fsp4HI GCNGC 9 cut(s) 42, 45, 48, 192, 354, 796, 822, 861, 981
FspBI CTAG 1 cut(s) 909
GlaI GCGC 1 cut(s) 342
GluI GCNGC 9 cut(s) 42, 45, 48, 192, 354, 796, 822, 861, 981
GsaI CCCAGC 2 cut(s) 353, 500
GsuI CTGGAG 2 cut(s) 196, 1295
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 1 cut(s) 335
HapII CCGG 1 cut(s) 720
HhaI GCGC 1 cut(s) 343
Hin1I GRCGYC 2 cut(s) 341, 399
Hin1II CATG 7 cut(s) 360, 368, 414, 592, 767, 1184, 1220
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 2 cut(s) 729, 937
HindII GTYRAC 2 cut(s) 729, 937
HinfI GANTC 6 cut(s) 18, 787, 806, 1024, 1125, 1148
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 720
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 6 cut(s) 362, 549, 729, 937, 1039, 1251
Hpy188I TCNGA 7 cut(s) 76, 136, 169, 457, 1054, 1232, 1258
Hpy188III TCNNGA 7 cut(s) 15, 213, 437, 529, 757, 1207, 1274
Hpy8I GTNNAC 6 cut(s) 362, 549, 729, 937, 1039, 1251
HpyAV CCTTC 2 cut(s) 32, 780
HpyCH4III ACNGT 5 cut(s) 484, 1036, 1071, 1283, 1305
HpyCH4IV ACGT 3 cut(s) 399, 567, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 184, 332, 818
HpyF3I CTNAG 4 cut(s) 183, 423, 975, 1257
HpySE526I ACGT 3 cut(s) 399, 567, 731
Hsp92I GRCGYC 2 cut(s) 341, 399
Hsp92II CATG 7 cut(s) 360, 368, 414, 592, 767, 1184, 1220
HspAI GCGC 1 cut(s) 341
KasI GGCGCC 1 cut(s) 340
Ksp22I TGATCA 1 cut(s) 1222
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 3 cut(s) 766, 1139, 1222
LguI GCTCTTC 1 cut(s) 309
LmnI GCTCC 3 cut(s) 455, 509, 679
Lsp1109I GCAGC 5 cut(s) 340, 782, 833, 872, 992
LweI GCATC 3 cut(s) 299, 532, 916
MaeI CTAG 1 cut(s) 909
MaeII ACGT 3 cut(s) 399, 567, 731
MaeIII GTNAC 2 cut(s) 649, 970
MalI GATC 3 cut(s) 768, 1141, 1224
MbiI CCGCTC 2 cut(s) 302, 450
MboI GATC 3 cut(s) 766, 1139, 1222
MboII GAAGA 7 cut(s) 63, 273, 285, 296, 641, 686, 1019
MflI RGATCY 1 cut(s) 1139
MlsI TGGCCA 1 cut(s) 335
MluCI AATT 5 cut(s) 216, 249, 256, 268, 1097
MluNI TGGCCA 1 cut(s) 335
Mly113I GGCGCC 1 cut(s) 341
MlyI GAGTC 2 cut(s) 1134, 1157
MmeI TCCRAC 3 cut(s) 99, 144, 726
Mox20I TGGCCA 1 cut(s) 335
MscI TGGCCA 1 cut(s) 335
MseI TTAA 3 cut(s) 309, 728, 1312
Msp20I TGGCCA 1 cut(s) 335
MspA1I CMGCKG 3 cut(s) 50, 353, 1168
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 1 cut(s) 720
MspR9I CCNGG 2 cut(s) 557, 615
MvaI CCWGG 2 cut(s) 557, 615
MwoI GCNNNNNNNGC 4 cut(s) 47, 184, 332, 818
NarI GGCGCC 1 cut(s) 341
NcoI CCATGG 1 cut(s) 588
NdeII GATC 3 cut(s) 766, 1139, 1222
NlaIII CATG 7 cut(s) 360, 368, 414, 592, 767, 1184, 1220
NlaIV GGNNCC 4 cut(s) 7, 342, 898, 957
NmuCI GTSAC 1 cut(s) 649
NspI RCATGY 1 cut(s) 360
PasI CCCWGGG 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 309
PfeI GAWTC 4 cut(s) 18, 787, 806, 1024
PflFI GACNNNGTC 1 cut(s) 1124
PkrI GCNGC 9 cut(s) 43, 46, 49, 193, 355, 797, 823, 862, 982
PleI GAGTC 2 cut(s) 1133, 1156
PluTI GGCGCC 1 cut(s) 344
PpsI GAGTC 2 cut(s) 1133, 1156
PshAI GACNNNNGTC 1 cut(s) 485
Psp6I CCWGG 2 cut(s) 555, 613
PspFI CCCAGC 2 cut(s) 349, 496
PspGI CCWGG 2 cut(s) 555, 613
PspN4I GGNNCC 4 cut(s) 7, 342, 898, 957
PstI CTGCAG 4 cut(s) 862, 970, 1120, 1138
PstNI CAGNNNCTG 3 cut(s) 827, 974, 1142
PsuI RGATCY 1 cut(s) 1139
PsyI GACNNNGTC 1 cut(s) 1124
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 3 cut(s) 309, 728, 1312
SatI GCNGC 9 cut(s) 42, 45, 48, 192, 354, 796, 822, 861, 981
Sau3AI GATC 3 cut(s) 766, 1139, 1222
SbfI CCTGCAGG 1 cut(s) 1138
SchI GAGTC 2 cut(s) 1134, 1157
ScrFI CCNGG 2 cut(s) 557, 615
SdaI CCTGCAGG 1 cut(s) 1138
SfaNI GCATC 3 cut(s) 299, 532, 916
SfcI CTRYAG 6 cut(s) 858, 966, 1032, 1108, 1116, 1134
SfoI GGCGCC 1 cut(s) 342
SmlI CTYRAG 2 cut(s) 308, 527
SmoI CTYRAG 2 cut(s) 308, 527
SpeI ACTAGT 1 cut(s) 908
Sse8387I CCTGCAGG 1 cut(s) 1138
Sse9I AATT 5 cut(s) 216, 249, 256, 268, 1097
SspDI GGCGCC 1 cut(s) 340
SspMI CTAG 1 cut(s) 909
StyD4I CCNGG 2 cut(s) 555, 613
StyI CCWWGG 2 cut(s) 336, 588
TaaI ACNGT 5 cut(s) 484, 1036, 1071, 1283, 1305
TaiI ACGT 3 cut(s) 402, 570, 734
TaqI TCGA 2 cut(s) 627, 1208
TasI AATT 5 cut(s) 216, 249, 256, 268, 1097
TauI GCSGC 4 cut(s) 44, 47, 50, 194
TfiI GAWTC 4 cut(s) 18, 787, 806, 1024
Tru1I TTAA 3 cut(s) 309, 728, 1312
Tru9I TTAA 3 cut(s) 309, 728, 1312
TscAI CASTG 2 cut(s) 269, 1041
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 5 cut(s) 353, 795, 821, 860, 980
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 3 cut(s) 453, 692, 1144
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 2 cut(s) 269, 1041
Tth111I GACNNNGTC 1 cut(s) 1124
Vha464I CTTAAG 1 cut(s) 308
XapI RAATTY 1 cut(s) 216
XceI RCATGY 1 cut(s) 360
XmiI GTMKAC 2 cut(s) 548, 1250
XspI CTAG 1 cut(s) 909
ZraI GACGTC 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.